减水减肥处理对露天菜田土壤磷分布的影响

以黑龙江省拉林露天菜田黑土为对象,对比分析常规水肥(WF)、减肥处理(W80%F)、减水处理(80%WF)、减水减肥配施秸秆处理(80%W80%F+S)下的土壤磷转化的变化,以期探究减水减肥处理下露天菜田土壤磷转化特征。结果表明:所有处理土壤全磷、有效磷垂直变化特征明显,呈现随着土壤深度增加而减少的趋势,且0~20 cm土层中的全磷、有效磷含量均高于其它土层,减水减肥配施秸秆处理显著降低了深层土中的有效磷含量(P<0.05),降低幅度为57.0%,抑制了磷向深层土移动。随着白菜生育的延长,减肥、常规水肥处理的土壤有效磷含量不断升高;而减水、减水减肥配施秸秆处理的土壤有效磷含量不断下降;减水、减肥、常规水肥处理的土壤有效磷含量在白菜生长的中后期达到最高后又缓慢下降。认为减水减肥配秸秆处理可减少土壤磷素淋溶的风险,降低淋溶液中全磷的含量,是对环境影响风险最小的可行水肥方案。     

机械制造企业B/S生产信息管理系统的设计

通过实例探讨了机械制造企业中B/S结构生产信息管理系统的基本功能要求,分析了生产信息管理系统中各子模块的相互关系和企业管理信息系统的信息流程关系;总结模块设计的要点,给出本系统中各子模块的详细供功能设计;提出了一种解决B/S结构生产信息管理系统中问题的方案。     

Effects of high fat/high cholesterol diet on differentially expressed gene of the liver in treble genes mutant mice

AIM: To study the effects of dietary factor on liver gene expression profile in apoE-/-/LDLR-/-/Leprdb/db mice and atherogenesis. METHODS: The gene expression profile was determined by using cDNA microarray. Total plasma TC and TG level were measured with COD-PAP and GPO-PAP methods respectively. The morphological characters of the aorta were also observed. RESULTS: Among the 4000 target genes, 78 and 114 genes were up-and down-regulated, respectively, in treble genes mutants fed with high fat high cholesterol diet compared with that of regular chow littermates, including lipid metabolism, carbohydrate, cell skeleton and immune related genes. Atherogenic diet induced severe plasma hyperlipidemia even in the early stage and also accelerated atherogenesis in the treble genes mutant mice. CONCLUSION: The data suggest that atherogenic diet aggravates abnormal plasma lipid metabolism and induces severe hyperlipidemia, and atherogenesis in the treble genes mutant mice as well.     

孤雌生殖诱导马铃薯双单倍体研究进展

诱导孤雌生殖是获得马铃薯双单倍体的有效方法,本文系统介绍了孤雌生殖诱导马铃薯双单倍体的研究技术,双单倍体的识别方法,二倍体授粉者在孤雌生殖诱导中的作用以及环境条件对孤雌生殖的影响等。并对该技术的发展进行了讨论。     

茎瘤固氮根瘤菌ORS571受体TlpA1对琥珀酸的感应机理

趋化作用在根瘤菌与宿主建立共生关系的起始阶段起着至关重要的作用。TlpA1是茎瘤固氮根瘤菌ORS571重要的跨膜趋化受体，但其感应机理尚未清楚。考察茎瘤固氮根瘤菌ORS571跨膜趋化受体TlpA1对趋化物的感应机理及生物学功能。通过平末端连接的方法构建N端表达菌株进行原核表达蛋白及纯化，利用等温滴定量热法（isothermal titration calorimetry）检测蛋白与趋化物的相互作用，测定野生型与突变株的生物膜及絮凝等表型。结果显示，跨膜趋化受体TlpA1通过胞外部分第29～183位氨基酸与琥珀酸结合。固氮条件下，突变株ΔTlpA1生物膜形成量相较于野生型下降约38%。絮凝量与野生型无显著性差异。跨膜趋化受体TlpA1通过感应琥珀酸调控茎瘤固氮根瘤菌ORS571生物膜的形成，对细胞絮凝无调控作用。     

基于可持续发展的农村水资源分配模式研究

农村水资源是影响我国社会主义新农村建设的主要因素之一。从可持续发展的角度出发,通过对我国的农村水环境和水资源现状的分析,提出基于可持续发展四大原则的“以供定需”农村水资源分配模式,并提出执行分配模式的农村水资源管理具体对策。     

Differential proteomic analysis of liver in apoE/LDLR double-gene mutant mice

AIM: To comparatively study the differential expression of protein profiles of liver between double lipid metabolism genes mutant (apoE-/-/LDLR-/-) and wild type（WT）mice.The key proteins related to atherosclerosis and dysfunction of lipid metabolism were also characterized.METHODS: Two-dimensional gel electrophoresis and mass spectrometry were used to analyze the differential displayed proteomics of 5-week-old double-gene mutants and wild type mice fed a regular chow for 2 weeks.RESULTS: Approximately (928±15) spots and (1 017±50) spots were detected in apoE-/-/LDLR-/- mice (n=3) and WT mice livers (n=3),respectively.The average matched ratio was 78.7% and 83.2%.The differential expression analysis showed that the matched spots between apoE-/-/LDLR-/- mice and WT mice existed.Compared with the wild type,108 spots were not matched in apoE-/-/LDLR-/- mice.10 over expression spots (>5 fold) and 45 lower expression spots (>5 fold) were noted.Six significant differential proteins in gel were identified by LTQ-ESI,e.g.endoplasmin precursor,acidic leucin-rich nuclear phosphoprotein 32 family member A,serotransferrin precursor,stress-70 protein precursor,fibronectin precursor,complement C3 precursor,fibrinogen gamma polypeptide.CONCLUSION: The protein profile of apoE-/-/LDLR-/- mouse liver exhibits significant difference compared to that of WT mice.The results imply that lipid metabolism relative polygenetic mutation contributes to the alteration of mouse liver protein expression profile,especially that lipid metabolism related perhaps participates in dysfunction in lipid metabolism during atherogenesis.     

焊接材料强度对高钢级管道环焊缝应变能力的影响

为了评价母材、根焊材料、填充焊材料的强度对X80管道环焊缝应变能力的影响,采用非线性有限元方法,以中俄东线直径1 422 mm、壁厚21.4 mm的高纲级管道为研究对象,考虑焊接接头完整几何形貌与性质有明显差异的4种材料分区,采用钥匙孔(Key Hole)模型准确模拟裂纹张开过程中裂纹尖端的钝化行为,研究了拉伸载荷作用下根焊内表面周向裂纹的起裂行为。计算结果表明:对于双V形坡口焊接接头,根焊材料强度对焊接接头整体应变能力影响很小,可以忽略不计;填充焊材料与母材强度的高强匹配对保证焊缝区应变能力最为重要,在难以提高填充焊材料强度的情况下,限定母材的强度指标是提高高钢级管道焊接接头应变能力的有效方法。(图7,表1,参25)     

日粮中添加高硒钴苜蓿草粉对家兔产品营养水平的影响

在新西兰大白兔日粮中添加10%的高硒、高钴、高硒钴等3种高微苜蓿草粉,研究其对新西兰大白兔产品品质和营养水平的影响。结果表明：在新西兰大白兔日粮中添加10%高硒和高硒钴苜蓿草粉,能显著提高家兔产品中肝脏、肾脏和心脏和中硒的含量,其中肝脏硒含量最高,其次为肾脏。随着硒量的增加,更多的硒首先富积在肝脏和肾脏中。而高硒钴苜蓿草粉比高硒苜蓿草粉更有利于硒在大白兔肝脏中富集。另外,添加高硒钴苜蓿草粉对肉中蛋白质、脂肪和碳水化合物含量影响不显著,但能显著提高其中Fe、Cu和Zn含量。     

激素调控促进动物生长发育的研究

激素对机体的代谢、生长、发育、繁殖、性别、性欲和性活动等起重要的调节作用。动物机体通过各种内分泌腺分泌的激素,间接调节动物机体的活动。内分泌腺分泌的激素直接进入血液、随着血液循环到达身体各个部分,在一定的器官或组织中发生作用,从而协调动物机体新陈代谢、生长、发育、生殖及其它生理机能,使这些机能得到兴奋或抑制,使它们的活动加快或减慢。生长激素可以促进蛋白质的合成与骨的生长,甲状腺激素促进新陈代谢和生长发育,可以提高神经系统的兴奋。胰岛分泌的激素可以控制糖类代谢,调节血糖含量。性激素促进生殖器官的生长发育,维持第二性征。