Effect of mitochondrial ATP sensitive potassium channels opening on translocation of protein kinase C epsilon in cultured adult rat ventricular myocytes

AIM: To investigate the effects of mitochondrial ATP sensitive potassium (MitoKATP) channel opening on translocation of protein kinase C epsilon (PKCε) and the relationship between the translocation of PKCε and the production of reactive oxygen species. METHODS: The expression of PKCε in cultured adult rat ventricular myocytes was investigated with immunofluorescence and Western blotting techniques. RESULTS: （1） Diazoxide, a selective MitoKATP channel opener, caused a significant translocation to myofibrillar-like structures in cultured adult rat ventricular myocytes. (2) The N-2-mercaptopropionylglycine (MPG), a free radical scavenger, partly inhibited the translocation of PKCε caused by diazoxide. (3) Chelerythrine, a selective protein kinase C (PKC) inhibitor, completely blocked the translocation of PKCε caused by diazoxide. CONCLUSION: Opening of MitoKATP channel might activate PKCε and make it translocation to myofibrillar-like structures. PKCε activation occurs downstream of MitoKATP channel, and might be caused by production of reactive oxygen species after opening of MitoKATP channel.     

大豆锈病研究现状与进展

大豆锈病近年在东南亚、南美洲发展迅速,对大豆生产威胁越来越大,尤其2004年11月登陆美国大陆,更引起大豆研究者、大豆生产国和进口国的极大关注。本文介绍了近年国际上大豆锈病的研究进展,主要包括大豆锈病的发现、地理分布、病原、症状、寄主范围、病原菌生理分化和防治技术研究等。     

黑皮中熟西瓜新品种开杂18号的选育

开杂18号是开封市农林科学研究所选育的黑皮、中熟西瓜新品种。全生育期103 d,果实发育期33 d,具有果个大、果皮黑且韧、品质优、易坐果、抗性强、适应范围较广等突出优点。     

藿香植物组织培养技术研究

选用藿香健壮枝条和叶片作为试验材料,进行组培技术的研究.结果表明:藿香取材季节以夏季较好,适宜的外植体种类为茎段.诱导愈伤组织形成的激素组合为6-BA 1.0mg/L NAA 0.3 mg/L 2,4-D 0.3 mg/L,具有愈伤组织生长速度快并易于分化;不定芽分化激素组合为6-BA 0.5 mg/L NAA 0.1 mg/L,继代培养组合6-BA 0.1 mg/L NAA 0.5 mg/L B90.3mg/L,增殖在8倍以上;生根培养组合1/2MS NAA0.6 mg/L,一般20 d左右生根,平均根量4～5条,生根率达92%.     

南阳牛IGF2基因第2外显子多态性及其与生长发育相关性研究

以126头纯种南阳黄牛为研究材料，利用PCR-RFLPs技术对胰岛素样生长因子2（IGF2）第2外显子的多态性及其与生长发育的相关性进行了分析。结果表明：南阳牛在该位点的遗传多态性丰富。检测到2种等位基因A和B，频率分别为0．6865和0．3135，A等位基因在群体中是优势等位基因；BB型个体的6、12、18、24月龄体斜长和胸围，12、18和24月龄的坐骨端宽，初生、6月龄和24月龄体重等体征性状显著地优于AA型；6月龄体斜长、12月龄的胸围、18月龄和24月龄坐骨端宽也显著优于杂合型。初步推断JGF2基因是非常有价值的辅助选择标记，可为南阳牛肉用型方向选择提供理论依据。     

美洲型猪繁殖与呼吸综合征病毒荧光RT-PCR检测方法的建立

参照美洲型猪繁殖与呼吸综合征病毒（PRRSV）M基因序列，设计引物和探针，并建立其荧光RT-PCR检测方法，以检测严重危害我国养猪业的此型PRRSV。该法能检测到1 TCID50的PRRSV。用此法检测猪瘟病毒、乙脑病毒、伪狂犬病毒、马动脉炎病毒、圆环病毒Ⅱ型和MARC145细胞的RNA，结果均为阴性。对126份临床样品进行检测应用，检测结果（7份阳性，119份阴性）与病毒分离完全符合。此法中的阳性对照为体外转录的dsRNA，具有高度的稳定性，解决了此类检测方法所用的RNA阳性对照所普遍存在的易于降解的难题。     

Comparative phosphoproteome analysis of cardiomyocytes preconditioned by diazoxide

AIM: To analyze and identify the phosphoproteins associated with diazoxide preconditioning. METHODS: Proteomics technique was used to investigate the changes of phosphoprotein after diazoxide preconditioning. Adult rat ventricular myocytes were pretreated in the presence and absence of 200 μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched respectively from control and diazoxide pretreated groups were then separated by two-dimensional (2D) gel electrophoresis and stained with sliver staining kit. Phosphoproteins of interest were further identified by mass spectrometry. RESULTS: Associated with diazoxide preconditioning, the proteins of chaperonin containing TCP-1 and hypothetical protein XP_346548 were phosphorylated significantly. The proteins of 94 kD glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphorylated markedly (P<0.05). CONCLUSION: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in response to diazoxide preconditioned signaling, which may mediate myocardioprotection signaling downstream mitochondrial staining dish K_ATP channel induced by ischemic preconditioning.