Effects of Phosphorus Fertilizer Source and Plant Density on Growth and Yield of Maize in Northwestern Pakistan

ABSTRACT

Phosphorus (P) fertilizer source and plant density are considered some of the most important factors affecting crop growth and yield. A field experiment was conducted to determine the impact of P source [zero-P control, DAP (diammonium phosphate), SSP (single super phosphate), and NP (nitrphos)] and plant density (D₁ = 40,000, D₂ = 60,000, D₃ = 80,000, and D₄ = 100,000 plants ha^?1) on growth and yield of maize (Zea mays L cv. Azam) on a P-deficient soil (6.6 mg P kg^?1) at New Developmental Agricultural Research Farm, North-West Frontier Province (NWFP) Agricultural University, Peshawar, Pakistan, during summer 2006 in wheat-maize cropping system. Physiological maturity was delayed, plant height was increased and leaf area was decreased significantly when maize was planted at highest (D₄) than at lowest plant density (D₁). Application of SSP resulted in earlier physiological maturity of maize than other P fertilizers. Grain and stover yield, harvest index, shelling percentage, thousand grain weight and grains ear^?1 were maximized at D₃ (80,000 plants ha^?1) and with application of P fertilizer. Highest benefit in growth and grain yield was obtained with application of DAP to maize planted at D₃. Application of DAP at D₃ gave 15, 29, and 19% higher grain yield than its application at D₁, D₂, and D₄, respectively. In conclusion, the findings suggest that growing maize at 80,000 plants ha^?1 applied with DAP can maximize productivity of maize in the wheat-maize cropping system on P-deficient soils.     

Expression and Localization of Vascular Endothelial Growth Factor and its Receptors in the Corpus Luteum During Oestrous Cycle in Water Buffaloes (Bubalus bubalis)

The aim of this study was to document the expression and localization of VEGF system comprising of VEGF isoforms (VEGF 120, VEGF 164 and VEGF 188) and their receptors (VEGFR1 and VEGFR2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle. Real‐time RT‐PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors. In general, all the components of VEGF system (the VEGF isoforms and their receptors) were found in the water buffalo CL during the oestrous cycle. The mRNA as well as protein expression of VEGF system was highest during the early and mid‐luteal phase, which later steadily decreased (p < 0.05) after day 10 to reach the lowest level in regressed CL. As demonstrated by immunohistochemistry, VEGF protein was localized predominantly in luteal cells; however, VEGFR1 and VEGFR2 were localized in luteal cells as well as in endothelial cells. In conclusion, the dynamics of expression and localization of VEGF system in buffalo corpora lutea during the luteal phase were demonstrated in this study, indicating the possible role of VEGF system in the regulation of luteal angiogenesis and proliferation of luteal as well as endothelial cells through their non‐angiogenic function.     

Plant and soil responses to nitrogen and phosphorus fertilization of bromegrass‐dominated haylands in Saskatchewan,Canada

Field experiments were conducted at three different sites in Saskatchewan, Canada (Colonsay, Vanscoy and Rosthern) over two years (2005 and 2006) to determine the effects of dribble‐banded and coulter‐injected liquid fertilizer applied in the spring of 2005 at 56, 112 and 224 kg N ha^?1 with and without P at 28 kg P₂O₅ ha^?1. The three sites were unfertilized, 7‐ to 8‐year old stands of mainly meadow bromegrass (Bromus riparius)‐dominated haylands. All fertilization treatments produced significantly (P ≤ 0·05) higher dry matter yield than the control in the year of application at the three Saskatchewan sites. There was no significant difference between the two application methods (surface dribble band vs. coulter injected) for any fertilizer treatments. The addition of 28 kg P₂O₅ ha^?1 P fertilizer along with the N fertilizer did not have a significant effect on yield in most cases. In the year of application, increasing N rates above 56 kg N ha^?1 did not significantly increase yield over the 56 kg N ha^?1 rate in most cases, but did increase N concentration, N uptake and protein concentration. A significant residual effect was found in the high N‐rate treatments in 2006, with significantly higher yield and N uptake. In 2005, the forage N and P uptake in the fertilized treatments were significantly higher than the control in all cases. The N uptake at the three Saskatchewan sites increased with increasing N rate up to the high rate of 224 kg N ha^?1, although the percent recovery of applied N decreased with increasing rate. The P fertilization with 28 kg P₂O₅ ha^?1 also increased P uptake. Overall, rates of fertilizer of approximately 56 kg N ha^?1 appear to be sufficient to produce nearly maximum forage yield and protein concentration of the grass in the year of application.     

Yield and protein content of barley as affected by release rate of coated urea and rate of nitrogen application

Coated urea consists of a urea core and a polymer coating. It meters out urea over a period of time. In the market place, price is favorable for high protein content feed barley. The objectives of this study were to determine release rate of urea from coated urea products and relative effectiveness of urea, coated urea or a mixture of coated urea products with different release rates in increasing yield and protein content of barley. Release rate of coated urea Mini I (quick release) and Mini II (slow release) in water was determined at 23°C by recovering ten pre‐weighed granules from 500 mL water at 6 h, 2, 4, 6, 8, 10, and 12 days. The recovered granules were dried and then weighed. Barley (Hordium vulgare L. cv. Duke) was grown in potted soil (2 L) at 15°C for 90 days in a growth chamber with treatments of Nil, non‐coated urea, Mini I, Mini II, Mixture I (1/3 urea+1/3 Mini I+1/3 Mini II) and Mixture II (1/5 urea+2/ 5 Mini I+2/5 Mini II). The nitrogen (N) application rates were 100, 200 and 300 kg N ha^‐1. Above‐ground plant samples were taken at 22, 44, 66, and 90 (maturity) days after seeding, and dry matter mass per pot and N content of the plant samples were determined. The release of urea from Mini I and Mini II followed a lognomial pattern. Increasing N application rate increased dry matter yield of barley. Dry matter yield from urea tended to be higher than other treatments at each rate of N application, but that did not couple with high grain protein content. At 100 kg N ha^‐1, there was no post anthesis N assimilation (PANA) for urea and Mini I, but there were 4, 14, and 13% PANA for Mini II, Mixture I, and Mixture II, respectively. However, when N application rate was increased to 200 and 300 kg N ha^‐1, there was PANA even for urea treatment. Protein content of barley grain was higher with coated urea or mixture treatments than with urea at each rate of N application. The potential N loss (i.e., difference between percentN released from fertilizers and percent fertilizer N recovered by barley) was Mini II<Mini I<Mixture II<Mixture I for the same N application rate, and was 100<200<300 kg N ha^‐1 for the same fertilizer treatment. In conclusion, at a limited N application rate, coated urea with a slow release rate or a combination of two coated urea products (quick and slow release) with urea increased grain protein content of barley. The potential N loss was less with coated urea applied alone than with a mixture of coated urea and urea.     

NITRATE IN LEAF PETIOLE AND BLADE OF SPINACH CULTIVARS AND ITS RELATION TO BIOMASS AND WATER IN PLANTS

A pot experiment was carried out, with 30 spinach cultivars to determine nitrate accumulation in leaf blade and petiole, and its relationship to biomass and water in plants. Results showed that the fresh weight proportion of blade to shoot was higher than that of petiole. Furthermore, a higher positive correlation was found between fresh weights of blades and shoots than that of petioles and shoots. Unlike biomass, nitrate-nitrogen (N) concentration and total amount of nitrate-N accumulated in petiole were significantly higher than those in blade, and petiole was obviously the main organ for nitrate accumulation. Differences of nitrate-N concentration in petiole and the observed positive correlation between nitrate-N concentrations in petioles and shoots were more significant than that in blades and shoots. Nitrate-N concentration in petiole was also significantly correlated with fresh and dry shoot weight and total amount of water in shoots. However, this relationship was not found for blade.     

Ultrastable mesostructured silica vesicles

A family of mesoporous molecular sieves (denoted MSU-G) with vesiclelike hierarchical structures and unprecedented thermal (1000 degreesC) and hydrothermal stabilities (more than 150 hours at 100 degreesC) associated with high SiO4 cross-linking was prepared through a supramolecular assembly pathway that relies on hydrogen bonding between electrically neutral gemini surfactants of the type CnH2n+1NH(CH2)2NH2 and silica precursors derived from tetraethylorthosilicate. The vesicle shells are constructed of one or more undulated silica sheets that are about 3 nanometers thick with mesopores (average diameters from 2.7 to 4.0 nanometers) running both parallel and orthogonal to the silica sheets, which makes the framework structure bicontinuous and highly accessible. Catalytic metal ion centers [for example, Ti(IV) and Al(III)] have been incorporated into the framework with the retention of hierarchical structure.     

Selection for brown stripe downy mildew resistance in maize

Summary The maize (Zea mays L.) cultivar Makki Safed 1 (MS1) with susceptibility to brown stripe downy mildew (BSDM) caused by Sclerophthora rayssiae var. zeae Payak & Renfro, was subjected to two cycles of mass selection and one cycle of full-sib family selection. Selection was carried out primarily for BSDM resistance.The mass selection was practised under artificial epiphytotic conditions in a disease nursery. Full-sib progenies and performance trials on MS1 and its improved versions were grown in diseased and disease free environments.Mass selection resulted in a significant improvement for resistance to BSDM. A cycle of full-sib selection resulted in an additional improvement for resistance to the disease. The disease rating of the improved version was 1.5 against 4.5 for the original population (scale: 1, highly resistant to 5, highly susceptible). The yield of the improved populations of MS1 was significantly greater than that of MS1 in the disease nursery. In disease free experiments, the improved populations showed almost no yield advantage over MS1. There were also no significant differences between the original population and the improved population after three selection cycles for ear length, ear girth, number of kernel rows per ear, number of kernels per row, 1000-kernel weight, plant height, ear height and days to silk.     

Tillage, nitrogen and crop residue effects on crop yield, nutrient uptake, soil quality, and greenhouse gas emissions

Management practices that simultaneously improve soil properties and yield are crucial to sustain high crop production and minimize detrimental impact on the environment. The objective of this study was to determine the influence of tillage and crop residue management on crop yield, N uptake and C removal in crop, soil organic C and N, inorganic N and aggregation, and nitrous oxide (N₂O) emissions on a Gray Luvisol (Boralf) soil near Star City, Saskatchewan, Canada. The 4-year (1998–2001) field experiment was conducted with two tillage systems: no tillage (NT), and conventional tillage (CT); two levels of straw: straw retained (S), and straw removed (NS); and four rates of fertilizer N: 0, 40, 80, and 120 kg N ha⁻¹, except no N to pea phase of the rotation. The plots were seeded to barley (Hordeum vulgare L.) in 1998, pea (Pisum sativum L.) in 1999, wheat (Triticum aestivum L.) in 2000 and canola (Brassica napus L.) in 2001. Tillage and straw treatments generally had no effect on crop yield during the first three years. But in 2001, NT produced 55, 32, and 20% greater canola seed, straw and chaff than CT, respectively, whereas straw retention increased seed and straw yield by 33 and 19% compared to straw removal. Seed, straw and chaff yield of canola increased with N rate up to 40 kg N ha⁻¹, and root mass (0–15 cm depth) with N rate to 80 kg N ha⁻¹. Amount of N uptake and C removed in wheat and canola generally increased with N rate, but tillage and straw management had no consistent effect. After four crop seasons, total organic C (TOC) and N (TN), light fraction organic matter (LFOM), C (LFC), and N (LFN) were generally greater with S than NS treatments. Tillage did not affect TOC and TN in soil, but LFOM, LFC, and LFN were greater or tended to be greater under NT than CT. There was no effect of tillage, straw and N fertilization on NH₄-N in soil, but CT and S tended to have higher NO₃-N concentration in 0–15 cm soil than NT and NS, respectively. Concentration of NO₃-N increased substantially with N rate ≥80 kg ha⁻¹. The NT + S treatment had the lowest proportion (34%) of wind-erodible (<0.83 mm diameter) aggregates and greatest proportion (37%) of larger (>12.7 mm) dry aggregates, compared to highest (50%) and lowest (18%) proportion of corresponding aggregates in CT + NS, indicating less potential for soil erosion when tillage was omitted and crop residues were retained. Amount of N lost as N₂O was higher from N-fertilized than from zero-N plots, and it was substantially higher from N-applied CT plots than from N-applied NT plots. Retaining crop residues along with no-tillage improved soil properties and may also be better for the environment.     

Small grains stubble burning and tillage effects on soil organic C and N, and aggregation in northeastern Saskatchewan

Field experiments were conducted over 5 years (2000–2004) at two sites (Star City and Birch Hills) in the Saskatchewan Parkland region to determine the effects of tillage and crop residue burning on soil total organic C (TOC), total organic N (TON), light fraction organic matter (LFOM), light fraction organic C (LFOC), light fraction organic N (LFON) and dry aggregation. Two tillage (ZT, zero tillage; CT, conventional tillage, with one tillage in autumn and another in spring) and two burning (B, residue burnt in autumn; NB, residue not burnt and returned to the soil) treatments were combined in a barley (Hordeum vulgare L.)–canola (Brassica napus L.) rotation. After five crop seasons, the mass of TOC and TON in the 0–15 cm soil tended to be greater, whereas mass of LFOM, LFOC and LFON was significantly greater in NB than B treatments at both sites. Zero tillage resulted in greater TOC, TON, LFOM, LFOC and LFON in soil than CT, in both B and NB treatments. The mass of TOC, TON, LFOM, LFOC and LFON in soil was the highest in the ZT–NB treatment, and lowest in the CT–B treatment. Zero tillage had a lower proportion of fine aggregates (<0.83 mm diameter) and a greater proportion of large aggregates (>6.4 mm diameter) at both sites, but the mean weight diameter (MWD) was greater under ZT than CT only at Birch Hills. Although the tillage × burning interaction was not significant in most cases, the ZT–NB treatment usually had the lowest proportion (22.6%) of fine aggregates and the greatest proportion (47.1%) of large aggregates, compared to the highest (34.9%) and the lowest proportion (35.6%) of these aggregates, respectively, in CT–B treatment. This indicated reduced potential for wind erosion when tillage was omitted (ZT) and crop residues were returned to the soil (NB). Returning crop residue to soil rather than burning usually increased soil organic C and N, and aggregation, but the differences between treatments were of greater magnitude between tillage treatments (ZT versus CT) than between burning treatments (B versus NB). Overall, returning crop residues along with ZT improved soil organic C and N, and aggregation, while burning in combination with CT resulted in the deterioration of these soil properties.     

Long‐term effects of manure and fertilization on soil organic matter and quality parameters of a calcareous soil in NW China

Long‐term applications of inorganic fertilizers and farmyard manure influence organic matter as well as other soil‐quality parameters, but the magnitude of change depends on soil‐climatic conditions. Effects of 22 annual applications (1982–2003) of N, P, and K inorganic fertilizers and farmyard manure (M) on total organic carbon (TOC) and nitrogen (TON), light‐fraction organic C (LFOC) and N (LFON), microbial‐biomass C (MB‐C) and N (MB‐N), total and extractable P, total and exchangeable K, and pH in 0–20 cm soil, nitrate‐N (NO ‐N) in 0–210 cm soil, and N, P, and K balance sheets were determined using a field experiment established in 1982 on a calcareous desert soil (Orthic Anthrosol) at Zhangye, Gansu, China. A rotation of irrigated wheat (Triticum aestivum L.)‐wheat‐corn (Zea mays L.) was used to compare the control, N, NP, NPK, M, MN, MNP, and MNPK treatments. Annual additions of inorganic fertilizers for 22 y increased mass of LFON, MB‐N, total P, extractable P, and exchangeable K in topsoil. This effect was generally enhanced with manure application. Application of manure also increased mass of TOC and MB‐C in soil, and tended to increase LFOC, TON, and MB‐N. There was no noticeable effect of fertilizer and manure application on soil pH. There was a close relationship between some soil‐quality parameters and the amount of C or N in straw that was returned to the soil. The N fertilizer alone resulted in accumulation of large amounts of NO ‐N at the 0–210 cm soil depth, accounting for 6% of the total applied N, but had the lowest recovery of applied N in the crop (34%). Manure alone resulted in higher NO ‐N in the soil profile compared with the control, and the MN treatment had the highest amount of NO ‐N in the soil profile. Application of N in combination with P and/or K fertilizers in both manured and unmanured treatments usually reduced NO ‐N accumulation in the soil profile compared with N alone and increased the N recovery in the crop as much as 66%. The N that was unaccounted for, as a percentage of applied N, was highest in the N‐alone treatment (60%) and lowest in the NPK treatment (30%). In the manure + chemical fertilizer treatments, the unaccounted N ranged from 35% to 43%. Long‐term P fertilization resulted in accumulation of extractable P in the surface soil. Compared to the control, the amount of P in soil‐plant system was surplus in plots that received P as fertilizer and/or manure, and the unaccounted P as percentage of applied P ranged from 64% to 80%. In the no‐manure plots, the unaccounted P decreased from 72% in NP to 64% in NPK treatment from increased P uptake due to balanced fertilization. Compared to the control, the amount of K in soil‐plant system was deficit in NPK treatment, i.e., the recovery of K in soil + plant was more than the amount of applied K. In manure treatments, the recovery of applied K in crop increased from 26% in M to 61% in MNPK treatment, but the unaccounted K decreased from 72% in M to 37% in MNPK treatment. The findings indicated that integrated application of N, P, and K fertilizers and manure is an important strategy to maintain or increase soil organic C and N, improve soil fertility, maintain nutrients balance, and minimize damage to the environment, while also improving crop yield.