Effect of hPTTG1 siRNA on proliferation and apoptosis of ovarian cancer cells

AIM: To observe the proliferation and apoptosis of ovarian cancer cells by silencing the expression of human pituitary tumor-transforming gene 1 ( hPTTG1 ) using RNA interference technique.METHODS: The chemically synthesized siRNA targeting hPTTG1 was transfected into ovarian cancer cell line A2780 in vitro. The expression levels of hPTTG1 and c-myc were examined by RT-PCR and Western blotting. Cell proliferation was measured by MTT colorimetric assay and -TdR incorporation test. Cell apoptosis was detected by flow cytometry with annexin V/PI and TUNEL labeling.RESULTS: The expression of hPTTG1 at mRNA and protein levels was inhibited after transfection of hPTTG1 siRNA. The inhibitory efficiency was 70.5%±3.9% and 63.8%±4.5%, respectively. The absorbance began to decrease 24 h after transfection of hPTTG1 siRNA,and the highest inhibitory rate was 42.9%±5.2% at 48 h post-transfection. Radioactive incorporation of -TdR in hPTTG1 siRNA group was lower than that in normal and negative groups. The survival rate declined while the apoptotic rate and necrotic rate increased in hPTTG1 siRNA group. Apoptotic index in hPTTG1 siRNA group was higher than that in normal and negative groups. The expression of c-myc at mRNA and protein levels was down-regulated.CONCLUSION: Cell proliferation is inhibited and cell apoptosis is induced by hPTTG1 siRNA through down-regulating the expression of c-myc. hPTTG1 can be regarded as a candidate gene for ovarian cancer gene therapy.     

黑穗醋栗品种亲缘关系的ISSR分析

 采用ISSR标记技术对39个黑穗醋栗品种的亲缘关系进行分析。结果表明：13条ISSR引物扩增的总带数（A）从3到11条不等,平均扩增出6.77条带,平均多态性带百分比（P）为64.37%。聚类分析显示,在相似性系数为0.8496处将39个黑穗醋栗品种分为5组。第Ⅰ组包含8个起源国家的品种,说明世界各国之间基因交流较为频繁。‘Ben Lomond’和‘Kantata’同其他品种的遗传距离较远,两者又有诸多优良农艺性状,可作为黑穗醋栗的优良育种材料。     

Association between ERK5 -322G/T polymorphism and genetic susceptibility to sporadic colorectal cancer

AIM: To investigate the correlation between extracellular signal-regulated kinase 5 ( ERK5 ) -322G/T polymorphism (rs3866958) and the susceptibility to sporadic colorectal cancer (CRC) in southern Chinese population. METHODS: ERK5 -322G/T genotypes were determined by Taqman-MGB probes in 835 CRC cases and 908 healthy controls. RESULTS: No significance of ERK5 -322G/T genotype distribution between CRC patients and controls was observed, but -322G/T decreased the susceptibility to CRC in fat people whose BMI was ≥ 24 kg/m². Compared to GG genotypes, the carriers with GT and TT genotypes had a significant decrease in the risk of CRC(OR=0.576,95%CI 0.413-0.804, P<0.01). CONCLUSION: ERK5 -322G/T polymorphism (rs3866958) has no significant relevance with sporadic CRC susceptibility, but decrease, the risk of CRC in people with fatness. The T variant genotype is an independent protective factor against sporadic CRC of overweight patients in southern Chinese population.     

Inhibitory effect of 1,25-(OH)2D3 on proliferation of airway smooth muscle cells from passively-sensitized patients

AIM: To investigate the effects of 1,25-dihydroxyvitamin D₃ on the proliferation of passively-sensitized human airway smooth muscle cells (HASMCs), and to explore its potential role in asthmatic airway remodeling.METHODS: HASMCs were passively sensitized with 10% serum from asthmatic patients.1,25-(OH)₂D₃ was used as the interventor.The effect of 1,25-(OH)₂D₃ on the cell proliferation and its optimal concentration were determined by MTT colorimetric assay.The cell cycle analysis was performed by flow cytometry.The expression of proliferating cell nuclear antigen (PCNA) was measured by the method of immunocytochemical staining.RESULTS: 1,25-(OH)₂D₃ at the concentrations of 10^-9-10^-7 mol/L markedly inhibited the cell proliferation and the maximum effect was observed at the concentration of 10^-7 mol/L.This concentration of 1,25-(OH)₂D₃ markedly suppressed the PCNA-positive rate and hampered the G₁/S transition in HASMCs passively-sensitized by asthmatic serum.CONCLUSION: 1,25-(OH)₂D₃ has direct inhibitory effects on the proliferation of passively-sensitized HASMCs in vitro, which may be concerned with the beneficial role of 1,25-(OH)₂D₃ on the prevention and therapy of asthmatic airway remodeling.     

甘蓝黑腐病生理小种划分及其抗病性鉴定研究进展

甘蓝黑腐病是十字花科作物的主要病害之一,其病原菌生理小种的划分和抗病性鉴定的方法直接关系到甘蓝抗病育种工作的开展和进程。本文就这两个方面对国内外的研究进展进行了全面综述,并对今后的研究方向进行了展望。     

不同番茄品种氮素吸收利用的差异

以吉林省市场上常见的25个番茄品种为材料,研究了同一氮素水平不同番茄品种氮素吸收利用差异。结果表明：番茄总吸氮量和氮素利用效率的变幅分别为262.8～460.1 kg?hm-2和281～526 kg?kg-1。聚类分析表明,吸氮能力较弱、氮素利用效率较高、产量高的品种有4个：朝研219、瑞琦1号、四季粉红、佳粉19,产量平均值为145 754 kg?hm-2。相关分析及通径分析表明,总吸氮量与番茄产量呈显著正相关,并对提高番茄产量起主导作用。     

复硝酚钠和DA-6对生姜生长及产量品质的影响

在大田栽培条件下,研究了新型植物生长调节剂复硝酚钠、DA-6对生姜生长、产量及品质的影响。结果表明,复硝酚钠、DA-6均可显著促进生姜的生长,但生长前期以促进根系生长的效果最为显著|随着生长的进行,生姜植株各器官鲜质量亦显著高于对照。生姜收获时,复硝酚钠、DA-6处理的生姜株高分别比对照增加11.59%和14.02%,分枝数分别增加24.59%和28.69%,产量分别提高18.53%和22.30%,但复硝酚钠、DA-6处理间差异不显著。此外,复硝酚钠、DA-6还不同程度地提高了生姜根茎淀粉、可溶性蛋白及氨基酸含量。     

小样本情况下需水量预测模型研究

针对传统的GM（1,1）模型,分析了其预测结果与实际过程存在的偏差,通过GM（1,1）模型与自回归滑动平均模型相结合的方法以弥补偏差。以钦州市1999-2009年及2005-2009年城市供水总量这两组基础数据对本文所建模型进行验证,并利用此模型预测了钦州市2010-2018年需水量。通过与其他预测模型的对比,进一步证...     

基于GIS的可视化配电网线损研究

论述了国内外的线损研究工作和电力GIS的发展、应用现状及技术,对目前多种理论线损计算方法进行了分析,针对农村电网传统线损管理系统的不足和农业电力部门的迫切需要,基于GIS技术实现线损管理功能的思路、技术方法及其总体结构、数据模型和线损计算等展开了深入研究和探讨,并进行了具体实践研究。     

不同配比施肥对华北花岗片麻岩山地土壤促成的作用

华北花岗片麻岩山区土壤严重退化,恶劣的土壤环境是该地区植被恢复的限制性因子.利用不同配比的化肥对试验区内土壤进行促成技术研究,结果表明:各种配比的肥料间,对土壤有机质和营养元素的影响(以全P释放量为例),以NPK1和NPK2处理效果最好,其分别提高风化速率29.68%和31.21%;对土壤粒径的影响(以<1 mm粒径土壤重量百分比为例),同样以NPK1和NPK2处理效果最好,其分别提高风化速率30.50%和24.81%.综上分析可以说明,各种配比的化肥对土壤促成起到了一定的作用,试验区内土壤正朝成熟土壤方向演化.