围封、浅耕翻处理对退化天然羊草草甸草地土壤物理性状的影响

在河北沽源张家口农牧交错带研究了围封、浅耕翻改良措施对退化天然羊草草句草地土壤物理性状的影响.结果表明,与自由放牧相比,围封、浅耕翻处理均可以明显改善草地土壤物理性状,提高了土壤含水量、土壤总空隙度、>0.25 mm粒径的水稳性团聚体的含量及团聚体结构稳定系数,显著降低了土壤容重;与浅耕翻处理相比,围封处理对退化羊草草甸草地土壤物理性状的改善最为有利.     

中药新制剂菌痢消颗粒对猪鸡细菌性下痢的疗效观察

以人工复制细菌性下痢的猪、鸡为模型,采用不同剂量的菌痢消颗粒进行了疗效观察.从疗效来看,高、中剂量组的有效率显著高于阳性对照组(P≤0.05).从治愈率来看,菌痢消颗粒高、中剂量组与低剂量组和阳性对照组差异显著(P≤0.05),高、中剂量组治愈率差异不显著(P＞0.05).结果表明,菌痢消颗粒能安全、有效地治疗猪、鸡细菌性下痢.     

塔落岩黄芪、细枝岩黄芪及其变异型的生理特性研究

本文研究了毛乌素沙漠地区塔落岩黄芪和细枝岩黄芪及其变异型的生理学特性。结果表明,塔落岩黄芪和细枝岩黄芪各与其变异型之间在生理特性方面存在着极其显著的差异。     

Efficacy of the immunoblot assay for cysticercosis in pigs and modulated expression of distinct IgM/IgG activities to Taenia solium antigens in experimental infections

A recently invented immunoblot assay for human cysticercosis was evaluated for efficacy in pigs. The test population consists of 45 pigs with parasitologically confirmed cysticercosis, 47 with heterologous infections, 45 SPF or concrete raised control animals. With this group of 137 animals the test performance was 100% sensitive and 100% specific. The antigen-specific responses of immunoglobulin A (IgA), IgG and IgM in four pigs infected with Taenia solium eggs derived from a human were quantified by immunoblot. Antigen-specific activities were observed as early as 1 week postinfection. The first antigen-specific isotypic response was IgM antibodies directed against a glycoprotein at 97 KD (GP97). This activity generally disappeared between the sixth and ninth week postinfection. Between Weeks 5 and 8, IgG activity rose as IgM activity fell. The IgG activity, however, was directed mostly towards GP50 and GP42 antigens. If the same response occurs in people with cysticercosis, identifying specific isotype activity may help to distinguish new infection from old.     

种毒接种剂量与鸡胚尿囊液量的相关性研究

对分成3组而同机孵化的180个9日龄罗曼蛋鸡鸡胚分别尿囊腔接种NDVLasota种毒液0.1mL／个,0.15mL／个和0.20mL／个。结果：尿囊液的收集量与种毒接种量呈显著负相关,而血凝价受接种剂量的影响不显著。     

Vero E6细胞酵母双杂交cDNA文库的构建及鉴定

To seek out proteins which interact with structural proteins of porcine epidemic diarrhea virus （PEDV） in Vero E6 cells and study the mechanisms of viral infection.We created a Vero E6 cDNA yeast hybrid library. The total RNA of Vero E6 was extracted using Trizol method, and double strands cDNA was synthesized by SMART technique. We created a Vero E6 cDNA library by using homologous recombination in yeast. The results showed that the titer of cDNA library was 9.7×10⁸ CFU with the average of inserted fragments about 1.6 kb, and the recombination rate was 87%. These data indicated that the yeast two-hybrid cDNA library of Vero E6 was successfully constructed and might be useful for screening the host proteins interacting with structure protein of PEDV.     

蒙古族草原游牧文化(Ⅵ)——蒙古族的游牧生活与文体文化

通过查阅资料和调查总结了蒙古族的游牧生活与文体文化。蒙古族牧民乌琦大皮极为适应高原日温差大的自然环境,耐贮藏富营养的肉、奶、面食品和蒙古包最适合游牧生活方式。总之,蒙古族牧民的衣、食、住、行都与环境相适应。蒙古族牧民能歌善舞,创造了称为"草原文化三宝"的长调、马头琴、呼麦。其中,蒙古族长调民歌被联合国教科文组织列为"人类口头和非物质遗产代表作"。中国第1个文化遗产日颁布的第一批非物质文化遗产名录中,就有蒙古族长调民歌、呼麦、格斯尔、乌力格尔、安代舞、达斡尔族鲁日格勒舞、蒙古族马头琴音乐、成吉思汗祭典、鄂尔多斯婚礼、祭敖包、那达慕等17项。蒙古族的竞技体育活动包括骑马、摔跤、射箭、赛跑、游泳、抛套索"等,这些活动也与狩猎、游牧活动密切相关。蒙古族牧民创造的鹿棋、蒙古象棋、嘎拉哈、古尔等游戏丰富了中华牧业文化遗产宝库。     

NaCl胁迫下黑麦草种子萌发过程中DNA甲基化与基因表达分析

为了解黑麦草（Lolium perenne）种子在正常条件与NaCl胁迫下萌发过程中DNA甲基化动态变化以及重要盐胁迫相关基因的表达。运用甲基化敏感扩增多态性技术分析对照和150 mmol·L^-1 NaCl处理下黑麦草种子萌发过程（0,1,2,4,7 d）的DNA甲基化水平及动态变化,并通过实时荧光定量 PCR（QRT-PCR）检测8个重要的盐胁迫相关基因的表达情况。结果表明：黑麦草种子萌发过程中甲基化水平呈下降趋势,以双链甲基化方式为主,甲基化变化同时发生在编码序列和非编码序列中。NaCl处理下DNA甲基化程度高于CK,而去甲基化程度低于CK,最终导致NaCl处理下基因组净的甲基化位点数目增加。黑麦草种子耐盐萌发过程中代谢增强,8个盐胁迫相关基因表达量呈上升趋势,而NaCl胁迫延缓了种子萌发进程,在大多数时间点的基因表达低于对照。     

畜牧企业要什么样的企业精神

要想使企业精神真正成为一种支撑企业长远发展的精神力量,它需要设计,也需要持续保养,两者缺一不可。