Unilateral arytenoid lateralisation: An assessment of technique and long term results in 62 dogs with laryngeal paralysis

Sixty-two dogs with laryngeal paralysis were presented over a three year period. Presenting clinical signs included inspiratory stridor (100 per cent), exercise intolerance or syncopal episodes (81 per cent), loss or alteration in phonation (48 per cent) and coughing or gagging when eating (29 per cent). Causes of the paralysis were determined as traumatic (two dogs), neoplastic (two dogs), iatrogenic (two dogs), congenital (one dog) and idiopathic (55 dogs). Unilateral arytenoid lateralisation was performed in all dogs. The perioperative complication rate was approximately 10 per cent, while the success rate as judged by owners one year postoperatively was greater than 90 per cent. The technique avoided many of the recorded complications of intralaryngeal surgery. Operative times were short and the requirement for postoperative monitoring was minimal. Increasing familiarity with the technique favourably influenced the incidence of complications and the success rate.     

Restriction fragment length polymorphism for the Yc subunit gene of rat liver glutathione S-transferase

An altered expression of the Yc subunit gene of rat glutathione S-transferase (GST) in the liver of the LEC rat, which is a mutant strain with spontaneous hereditary hepatitis associated with severe jaundice, has been reported. To provide further information concerning the structure of the Yc subunit gene, we carried out the Southern blot hybridization analysis of DNA samples from rats of eight different inbred strains including LEC with cDNA complementary to mRNA specific for the Yc subunit of rat liver GST as a probe. The hybridization patterns of the DNA samples from rats belonging to the different inbred strains showed interstrain variation in the length of restriction fragments with four restriction endonucleases. Since the DNA samples prepared from several rats of one inbred strain gave an identical hybridization pattern, the restriction fragment patterns for the Yc gene could be used as markers for genetic monitoring of inbred rat strains. Although the altered expression of Yc-Yc activity of GST has been observed in the liver of the LEC rat, the characteristic changes in the gene structure of the Yc subunit of LEC rat were not detected in the present hybridization analysis.     

Effect of immunization against somatostatin in the pregnant ewe on growth and endocrine status of the neonatal lamb

Absorption of somatostatin (SRIF) specific antibodies from colostrum of ewes actively immunized against SRIF may improve growth rate of the neonatal lamb by neutralizing the inhibitory effects of SRIF on pituitary and thyroid function. Growth and endocrine parameters in the offspring of SRIF immunized (SI) and control (C) crossbred ewes were examined. Lamb weight was recorded at birth and twice each week to 24 days of age. Blood samples were collected prior to first suckle and twice each week. At 21 to 24 days of age, in separate experiments, lambs were infused with glucose (0.29 g/kg), arginine (0.25 g/kg) or thyrotropin-releasing hormone (TRH; 0.33 microgram/kg). A strong correlation (R = 0.88; P less than .01) was observed between anti-SRIF titre in the ewe at parturition and in the lamb at 3 days of age. No effect on lamb birth weight (SI 4.28 +/- 0.27 kg; C 4.35 +/- 0.23 kg) was observed. At 24 days of age cumulative gain in SI lambs (5.4 +/- 0.32 kg) was greater (P less than .05) than in C lambs (4.5 +/- 0.32 kg). The growth hormone secretory responses to glucose or arginine were not affected by treatment. Plasma IGF-I, plasma thyroxine (T4) and the plasma thyrotropin and T4 responses to TRH were not different between treatments. Plasma triiodothyronine (T3) was higher (P less than .05) in SI (2.46 +/- .10 ng/ml) than in C (2.01 +/- .05 ng/ml) lambs, however, the plasma T3 response to TRH was lower in SI lambs. Plasma glucose (mg/dl) was higher (P less than .05) in SI (118.4 +/- 1.7) than in C (106.0 +/- 4.0) lambs. Plasma insulin was not affected by treatment. Increased plasma T3 and glucose concentrations during SRIF immunoneutralization in the neonate lamb may be important factors contributing to the growth response observed.     

Mechanical Properties of Four Suture Patterns for Transected Equine Tendon Repair

Thirty-six superficial digital flexor tendons from nine fresh equine cadavers were transected and sutured with size 2 monofilament nylon. Nine tendons were repaired with each of four suture patterns: single-locking loop, double-locking loop, triple-locking loop, or three-loop pulley. The times required for application, tensile strengths, resistance to distraction (gap), and modes of failure were analyzed. The mean times required were: single-locking loop, 3 mins, 15 secs; double-locking loop, 4 mins, 15 secs; triple-locking loop, 10 mins, 50 secs; and three-loop pulley, 4 mins. The double-locking loop, triple-locking loop, and three-loop pulley suture patterns were stronger than the single-locking loop. The triple-locking loop and three-loop pulley patterns were close in strength and only the triple-locking loop was stronger than the double-locking loop. The three-loop pulley had the greatest resistance to gap formation and its mode of failure was different from the others. The three-locking loop suture patterns failed by suture breakage but the three-loop pulley failed first by suture pull-out and then by suture breakage.     

A grading system for lymphocytic plasmacytic colitis in dogs

Colonic mucosal samples were obtained every 4 weeks for 13 months from 6 clinically normal dogs and from 47 dogs with a clinical diagnosis of chronic inflammatory bowel disease. All samples were graded on a scale of 0-5, based upon the quantity of lymphocytes and plasma cells in the lamina propria, epithelial changes, and the presence of ulcers and erosions. A grade of less than or equal to 2.0 was considered normal and was assigned to 77 of 78 samples from clinically normal dogs and 28 of 48 samples from dogs with diarrhea. A transient increase in cellularity was noted in 1 sample from 1 control dog. Nineteen dogs with clinical disease had obvious histologic abnormalities. The grading scheme described provides the pathologist with an objective criterion for the microscopic evaluation of colonic mucosal samples obtained by endoscopic techniques and offers clinicians a method of assessing the dog's progress and response to therapy.     

Prevalence of domestic animal trypanosomiasis in the Fontem sleeping sickness focus, Cameroon

Three hundred-fourteen domestic animals: 114 goats, 93 sheep, 67 dogs and 30 pigs in the Fontem sleeping sickness focus (Cameroon) were screened for infection with trypanosomes using parasitological and serological tests. Nannomonas was the only sub-genus detected in the animals with a prevalence of 28.3%. The card agglutination test (Testryp CATT) for trypanosomiasis showed a positivity rate of 38.2%, indicating that it allowed a better estimation of animal trypanosomiasis. The absence of trypanosomes of the sub-genus Trypanozoon seems to indicate that human African trypanosomiasis is not a zoonosis in this sleeping sickness focus.     

Immunologic factors related to survival and performance in neonatal swine

Logistic regression was used to develop models predicting preweaning survival in 334 neonatal swine. Measured risk factors included birth weight, litter size (live born), dam parity, serum IgG concentration, serum ELISA titers recognizing common gram-negative core antigens, and serum concentrations of the third component of complement. Larger birth weights were associated with increased probability of preweaning survival. The highest mortality was observed in litters with more than 12 pigs. Pigs with serum concentration of the third component of complement (C3) in the lowest stratum, less than 20% adult pooled C3 standard (APC3), had reduced mortality, compared with high (greater than 38% APC3) and middle (20 to 38% APC3) groups. Associations between all other variables, including total serum IgG concentration and preweaning survival were not significant. Few pigs had hypogammaglobulinemia, less than 3% of the study population had serum IgG concentrations less than 1 g/dl. Of all measured variables, only birth weight and dam parity were significant predictors of preweaning gain. Larger pigs and pigs born to third or greater parity dams had more preweaning gain than other pigs.     

A case of "watery mouth" in a New Zealand Romney lamb

Watery mouth was diagnosed as the cause of illness and subsequent death in a 3-day-old Romney lamb which had not received early colostrum. The history, clinical signs and post-mortem findings were typical of the disease as seen in other countries, including Great Britain. The diagnosis was based on the clinical signs and was supported by an absence of post-mortem findings of other specific neonatal diseases. The potential importance of watery mouth under conditions of intensive husbandry in New Zealand is discussed.