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101.
Ranjan K. Mohanta Kamal K. Singhal Amrish K. Tyagi Y. S. Rajput Shiv Prasad 《Tropical animal health and production》2010,42(3):431-438
The effects of feeding transgenic (Bt) whole cottonseed (WCS) were studied in lactating cows. Twenty multiparous crossbred
cows (Karan Swiss × Karan Fries) in early lactation were given a concentrate mixture containing 40% crushed delinted non-transgenic
(non-Bt) WCS, 2 kg wheat straw and green fodder ad lib for a 15-day adaptation period. Thereafter, the cows were divided in two similar groups of 10 each on the basis of milk yield,
body weight (BW) and date of calving. The non-Bt control group continued on same ration, while for the Bt group the non-Bt
WCS was replaced by transgenic WCS, in a feeding trial of four weeks. The diets provided a minimum of 2 kg cottonseed/cow/d.
Mean DMI/100 kg BW and milk yield of non-Bt and Bt groups was 3.48 and 3.45 kg and 11.4 and 12.0 kg/d, respectively. Intake
of nutrients, digestibility, milk production and body condition score (BCS) did not differ between the groups (P > 0.05),
but BW gain was higher (P < 0.05) in the Bt group than the non-Bt group, probably as a result of hoof problem in two cows
of non-Bt group, which when compared excluding two animals from each group did not differ significantly (P > 0.05). Transgenic
protein (Cry1C) was not detected in the weekly milk samples or in blood plasma at the end of the experiment, showing that
delinted WCS containing Cry1C protein can safely be fed to lactating cows. 相似文献
102.
D. Venkatachalam V. K. Dumka B. Ranjan 《Journal of veterinary pharmacology and therapeutics》2018,41(1):155-158
The objective of this study was to investigate the pharmacokinetics of cefquinome following single intramuscular (IM) administration in six healthy male buffalo calves. Cefquinome was administered intramuscularly (2 mg/kg bodyweight) and blood samples were collected prior to drug administration and up to 24 hr after injection. No adverse effects or changes were observed after the IM injection of cefquinome. Plasma concentrations of cefquinome were determined by high‐performance liquid chromatography. The disposition of plasma cefquinome is characterized by a mono‐compartmental open model. The pharmacokinetic parameters after IM administration (mean ± SE) were Cmax 6.93 ± 0.58 μg/ml, Tmax 0.5 hr, t½kα 0.16 ± 0.05 hr, t½β 3.73 ± 0.10 hr, and AUC 28.40 ± 1.30 μg hr/ml after IM administration. A dosage regimen of 2 mg/kg bodyweight at 24‐hr interval following IM injection of cefquinome would maintain the plasma levels required to be effective against the bacterial pathogens with MIC values ≤0.39 μg/ml. The suggested dosage regimen of cefquinome has to be validated in the disease models before recommending for clinical use in buffalo calves. 相似文献
103.
Ferguson AD Chakraborty R Smith BS Esser L van der Helm D Deisenhofer J 《Science (New York, N.Y.)》2002,295(5560):1715-1719
Siderophore-mediated acquisition systems facilitate iron uptake. We present the crystallographic structure of the integral outer membrane receptor FecA from Escherichia coli with and without ferric citrate at 2.5 and 2.0 angstrom resolution. FecA is composed of three distinct domains: the barrel, plug, and NH2-terminal extension. Binding of ferric citrate triggers a conformational change of the extracellular loops that close the external pocket of FecA. Ligand-induced allosteric transitions are propagated through the outer membrane by the plug domain, signaling the occupancy of the receptor in the periplasm. These data establish the structural basis of gating for receptors dependent on the cytoplasmic membrane protein TonB. By compiling available data for this family of receptors, we propose a mechanism for the energy-dependent transport of siderophores. 相似文献
104.
Avijit Ghosh Brahma Swaroop Dwivedi Dipak Ranjan Biswas Mahesh Chandra Meena Abhijit Sarkar 《Archives of Agronomy and Soil Science》2018,64(9):1290-1301
The aim of this study was to understand impacts of long-term (43 years) fertilization on soil aggregation, N accumulation rates and δ15N in surface and deep layers in an Alfisol. Soil samples from seven treatments were analysed for N stocks, aggregate-associated N in 0–30 cm and the changes in δ15N in 0–90 cm depths. The treatments were: unfertilized control (control); recommended N dose (N); recommended N and phosphorus doses (NP); recommended N, P and potassium doses (NPK); 150% of recommended N, P and K doses (150% NPK); NPK + 10 Mg FYM ha?1 (NPK + FYM) and NPK + 0.4 Mg lime ha?1 (NPK + L). Results revealed that plots under NPK + FYM had ~39% higher total N concentrations than NPK + L in 0–30 cm soil layers. In NPK + L, macro-aggregates had 35 and 11% and microaggregates had 20 and 9% lower δ15N values than NPK + FYM in 0–15 and 15–30 cm soil layers, respectively. However, plots receiving NPK + FYM had ~39% greater deep soil (30–90 cm) N accumulation than NPK + L. These results would help understanding N supplying capacity by long-term fertilization and assist devising N management strategies in sub-tropical acidic Alfisols. 相似文献