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71.
Bergamasco Rde C Zanin GM de Moraes FF 《Journal of agricultural and food chemistry》2005,53(4):1139-1143
Sulfluramid is an expensive active principle of insecticidal baits that is lost by volatilization during the pelletization of baits. To increase the thermal stability of sulfluramid, we tested its molecular encapsulation in beta-cyclodextrin (beta-CD), using molar ratios of 1:1 and 1:2 (sulfluramid:beta-CD), using the complex preparation techniques of coprecipitation and kneading. The physical mixture of sulfluramid and beta-CD was also tested for comparison. The products of complexation were characterized by differential scanning calorimetry, thermogravimetry, and derivative thermogravimetry, indicating the formation of a sulfluramid/beta-CD complex and showing that the release of the complexed sulfluramid occurs in the range of 270-300 degrees C, a temperature range that is well above the temperature at which sulfluramid sublimates (40 degrees C). This result warrants a reduced sulfluramid loss in the preparation of insecticidal baits. The preparation of the complex by kneading with molar ratio of 1:2 gave the highest yield of complex, about 64%, in relation to the theoretical maximum. 相似文献
72.
For the present and the foreseeable future the following options appear to be either usable or worth further exploration: mausolea; disposal in mines of various sorts, and perhaps in ice; in situ melt; and further chemical separations. The options are interdependent. It is too early to assess disposal in space, and disposal in the oceans remains unsafe for lack of adequate knowledge. Table 3 is a summary of the main ideas for which we have worked out (sometimes uncertain) costs. For the short term, ultimate disposal in deep mines is the best-developed plan. However, the related concept of in situ melt has significant advantages and should be realistically appraised. Further chemical separation with subsequent recycling of the actinides in a LMFBR should be investigated and implemented, for it would be universally beneficial; on the other hand, additional removal of strontium and cesium does not seem attractive. Thus, for the near future we make the following recommendations: 1) Provide temporary storage facilities to ensure that the projected commercial high-level wastes do not become a public hazard. The AEC adopts this view, and has stated an intention to construct such facilities. But because of the capriciousness of man and nature, a workable ultimate disposal scheme must be developed soon. 2) Fund other ultimate disposal schemes at the same rate as the salt mine project-say $1 million a year or more-to sharpen the technological issues, so that a decision can be reached in the next few years. The schemes should include (i) in situ melt, and the variation with a central repository; (ii) burial in mines other than salt mines (including Antarctic rocks and permanent ice); (iii) further chemical separation of actinides and recycling actinides in a LMFBR. 3) Maintain liaison with the developing space shuttle technology to insure that no opportunity is lost. The AEC has a commitment to hold safety foremost in its waste management program, but budget considerations and management priorities have downgraded the program. Past funding levels and management emphasis have yet to produce, after a decade and a half, one operational long-term storage facility-a sign of both commendable caution and inadequate work. If nuclear power is to resolve our energy needs in the coming decades, its benefits should not be delayed for lack of a viable management program for high-level wastes. 相似文献
73.
A map of the interactome network of the metazoan C. elegans 总被引:1,自引:0,他引:1
Li S Armstrong CM Bertin N Ge H Milstein S Boxem M Vidalain PO Han JD Chesneau A Hao T Goldberg DS Li N Martinez M Rual JF Lamesch P Xu L Tewari M Wong SL Zhang LV Berriz GF Jacotot L Vaglio P Reboul J Hirozane-Kishikawa T Li Q Gabel HW Elewa A Baumgartner B Rose DJ Yu H Bosak S Sequerra R Fraser A Mango SE Saxton WM Strome S Van Den Heuvel S Piano F Vandenhaute J Sardet C Gerstein M Doucette-Stamm L Gunsalus KC Harper JW Cusick ME Roth FP Hill DE Vidal M 《Science (New York, N.Y.)》2004,303(5657):540-543
74.
Rose DJ 《Science (New York, N.Y.)》1971,172(3985):797-808
75.
A stable cell line expressing a complementary DNA clone encoding the vesicular stomatitis virus glycoprotein fused and formed polykaryons at pH 5.5. The formation of polykaryons was dependent on the presence of glycoprotein anchored at the cell surface and could be prevented by incubation of cells with a monoclonal antibody to the glycoprotein. Fusion occurred at a pH 0.5 unit lower than that observed for cells infected with vesicular stomatitis virus. 相似文献
76.
77.
Redesigning metabolic routes: manipulation of TOL plasmid pathway for catabolism of alkylbenzoates 总被引:22,自引:0,他引:22
Increasing quantities of man-made organic chemicals are released each year into the biosphere. Some of these compounds are both toxic and relatively resistant to physical, chemical, or biological degradation, and they thus constitute an environmental burden of considerable magnitude. Genetic manipulation of microbial catabolic pathways offers a powerful means by which to accelerate evolution of biodegradative routes through which such compounds might be eliminated from the environment. In the experiments described here, a catabolic pathway for alkylbenzoates specified by the TOL plasmid of Pseudomonas was restructured to produce a pathway capable of processing a new substrate, 4-ethylbenzoate. Analysis of critical steps in the TOL pathway that prevent metabolism of 4-ethylbenzoate revealed that this compound fails to induce synthesis of the catabolic enzymes and that one of its metabolic intermediates inactivates catechol 2,3-dioxygenase (C23O), the enzyme that cleaves the aromatic ring. Consequently, the pathway was sequentially modified by recruitment of genes from mutant bacteria selected for their production of either an altered pathway operon regulator that is activated by 4-ethylbenzoate or an altered C23O that is less sensitive to metabolite inactivation. The redesigned pathway was stably expressed and enabled host bacteria to degrade 4-ethylbenzoate in addition to the normal substrates of the TOL pathway. 相似文献
78.
On the first day after hatching, domestic chicks were trained for 20, 60, 120, or 240 minutes with an imprinting stimulus. On the second day, they were all retrained for 60 minutes. The greater the chicks' experience on the first day, the lower the rate of incorporation of tritiated uracil into macromolecules in the anterior part of the forebrain roof on the second day. Such effects were not found in other brain regions, nor in any brain region of chicks that received similar treatment on the first day but were not retrained on the second. 相似文献
79.
Protein-metal ion binding site: determination with proton magnetic resonance spectroscopy 总被引:1,自引:0,他引:1
P I Rose 《Science (New York, N.Y.)》1971,171(971):573-574
Proton magnetic resonance spectra of aqueous gelatin were analyzed with respect to composition and molecular interactions. Aqueous gelatin complexes cobaltous ions in the pD range of approximately 3.5 to 7.5. Glutamyl and aspartyl side chains are shown to be the sites of binding. 相似文献
80.
Gas emissions and the eruptions of mount st. Helens through 1982 总被引:3,自引:0,他引:3
Casadevall T Rose W Gerlach T Greenland LP Ewert J Wunderman R Symonds R 《Science (New York, N.Y.)》1983,221(4618):1383-1385
The monitoring of gas emissions from Mount St. Helens includes daily airborne measurements of sulfur dioxide in the volcanic plume and monthly sampling of gases from crater fumaroles. The composition of the fumarolic gases has changed slightly since 1980: the water content increased from 90 to 98 percent, and the carbon dioxide concentrations decreased from about 10 to 1 percent. The emission rates of sulfur dioxide and carbon dioxide were at their peak during July and August 1980, decreased rapidly in late 1980, and have remained low and decreased slightly through 1981 and 1982. These patterns suggest steady outgassing of a single batch of magma (with a volume of not less than 0.3 cubic kilometer) to which no significant new magma has been added since mid-1980. The gas data were useful in predicting eruptions in August 1980 and June 1981. 相似文献