Induction of B cell unresponsiveness to noninherited maternal HLA antigens during fetal life

Patients who have received many transfusions become highly sensitized and develop antibodies against almost all HLA alloantigens, so that finding a cross-match negative kidney donor is difficult. A survey of those patients showed that 50 percent did not form antibodies against the noninherited maternal HLA antigens. Apart from the obvious clinical implications, the data indicate that a human equivalent of murine neonatal or actively acquired tolerance has now been identified.     

Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin

A novel toxin, NetB, has recently been identified in virulent avian Clostridium perfringens isolates and shown to be an essential virulence factor in a clinical necrotic enteritis isolate. To assess whether NetB is more generally associated with avian necrotic enteritis isolates we have screened a range of C. perfringens strains from geographically diverse locations for both the presence and expression of the netB gene. Forty-four isolates were derived from necrotic enteritis disease cases from Australia, Belgium, Denmark and Canada and 55 isolates from healthy chickens from Australia and Belgium. The majority of strains isolated from necrotic enteritis-affected birds were netB positive (70%) and there was an absolute correlation between the presence of netB and in vitro expression of the NetB protein. Only two of the C. perfringens isolates from healthy chickens carried netB. Sequencing of the netB gene from 23 positive isolates showed that NetB is highly conserved, with only one predicted amino acid (A168T) difference, in six isolates, compared to the published sequence. This change did not alter the in vitro activity of the NetB toxin. The gene encoding the recently discovered TpeL toxin was also screened using PCR and only found in a small proportion of NetB-positive isolates from diseased birds. A selection of NetB-negative isolates, originating from diseased birds, was unable to cause disease in a necrotic enteritis induction model. This study provides further evidence that NetB is important in pathogenesis and advances our current understanding of C. perfringens virulence factors in avian necrotic enteritis.     

Characterisation of virulent and benign strains of Bacteroides nodosus

The extracellular proteases of 395 isolates of B. nodosus from ovine, bovine and caprine foot lesions were classified as either thermostable or thermolabile. Stable protease was associated with one and unstable protease with four distinctive isoenzyme patterns, each pattern differentiated by the relative mobility of paired isoenzymes. Pathogenicity tests on 64 isolates showed a correlation between the production of stable protease and the production of virulent ovine footrot lesions. The mean values for total protease activity, twitching motility and colony diameter were significantly higher for virulent compared to benign isolates, but the range of values overlapped. SDS-PAGE whole-cell electrophoretic profiles of virulent isolates were similar to the profiles of some benign isolates.     

The thermostability of proteases from virulent and benign strains of Bacteroides nodosus

Protease enzymes, produced by Bacteroides nodosus strains isolated from animals with virulent and benign forms of ovine footrot, were partially purified by ultra-filtration, ion exchange chromatography and gel permeation chromatography. Each enzyme had a similar pH optimum, was inhibited by phenylmethylsulfonyl fluoride (PMSF), ethylene diamine tetraacetic acid (EDTA) and ethyleneglycot-bis-aminoethylether-N,N-tetraacetic acid (EGTA), but was not inhibited by 1,10-phenanthroline. The results suggest that these enzymes are serine proteases that require divalent cations for activity. The enzymes could be distinguished by their differential temperature stability and differing susceptibility to irreversible inactivation by EDTA. Both enzymes were stabilised by incubation in the presence of Ca2+, but the enzyme purified from the virulent isolate required less Ca2+ for maximum stability. These results suggest that the differential thermostability of the protease activity detected in virulence tests is an intrinsic property of the protease enzymes.     

《木材水运建筑安装工程预算定额》修订和《概、预算编制办法及费用定额》编制

1994年9月～1995年9月,由主编单位四周林业勘察设计研究院承担《木材水运建筑安装工程预算定额》的修订和《木材水运建筑安装工程概、预算编制办法及费用定额》的编制。定额的修定一方面要与相关定额水平协调,同时将工程实体消耗量和施工措施性消耗分开;贯彻政府有关文件精神并反映当前平均先进水平;保留(64)定额的框架只对章,节目和消耗量,基价作调整。 新编《木材水运建筑安装工程概、预算编制办法及费用定额》,以办法与费用定额合一,简化文字实用为原则,项目设置、费用定额符合政府部门最新规定。结合实际控制水平,按工程性质划分为7项生产性主体工程;5项附属性工程;2项非生产性工程和其它工程及费用两大部分。     

Equine perioperative fatalities associated with general anaesthesia at a private practice--a retrospective case series

OBJECTIVE: To determine the fatality rate of horses undergoing general anaesthesia at a private equine referral practice using a limited number of anaesthetic protocols. METHODS: A retrospective analysis of records (n = 17 961) from all horses undergoing general anaesthesia for surgical procedures from 1997 to 2001 at Rood and Riddle Equine Hospital, Lexington, Kentucky, USA. Results were reported as percentage of the population, and as crude mortality rates for each procedure (deaths per 1000). RESULTS: The prevalence of equine fatalities directly related to anaesthesia was 0.12% (n = 21) and this rose to 0.24% (n = 42) with the inclusion of horses killed or dying within 7 days post general anaesthesia. Causes of death directly related to anaesthesia were cardiac arrest (n = 10), fracture in recovery stall (n = 8), neuropathy and myopathy necessitating euthanasia (n = 3). Crude mortality rates per procedure were < or =7 deaths per 1000 cases, except arthrodesis/osteotomy cases were 66.7 deaths per 1000 cases. CONCLUSIONS: The anaesthetic fatality rate at this practice is lower than has been reported previously. CLINICAL RELEVANCE: Familiarity with an anaesthetic protocol in combination with reduced anaesthetic time, emergencies of shorter duration between diagnosis and surgery, and adequate preoperative examination appear to minimize the risks associated with general anaesthesia in horses.     

四川森林航测和林业遥感回顾与展望（上）

1953～1959年林业部航测队在四川省袭江下游、金沙江下游、青衣江上游以及川、陕、鄂交界的大巴山林区进行航测航调工作,1956～1958年林业部三、八森林经理调查大队使用航片在四川小金、会东等县及甘、青交界的祁连山林区完成323hm~2的森林经理调查;1959～1983年间森勘一大队使用航片调查总面积达1432万hm~2、森勘二大队调查1211万hm~2;营林调查队1959～1962年使用航片调查设计达128万hm~2。使用航片节省劳力提高工效,便于野外调查作业和小班区划调绘,不受地形限制以及有利于室内检核调绘质量等优点。放大航片象幅,蒙片调绘,内业航片加密,辐射隔网和透视格网转绘技术可提高航片实用性和精度     

Influence of water table decline on growth allocation and endogenous gibberellins in black cottonwood

Cottonwoods occur in riparian areas where water table depth generally varies with the elevation of the adjacent river. Plant adaptation to the riparian zone requires the coordination of root elongation to maintain contact with the water table during the summer decline. We investigated the effects of rate of water decline on growth allocation and concentrations of endogenous gibberellins (GAs) in black cottonwood (Populus trichocarpa Torr. & A. Gray ex Hook.) saplings. Rhizopods were used to achieve water decline rates of 0, 2 and 4 cm day(-1). Root elongation approximately doubled in response to the 2 cm day(-1) treatment, whereas leaf area was reduced. A water decline rate of 4 cm day(-1) led to water stress, as evidenced by reduced growth, increased leaf diffusive resistance, decreased water potential, and leaf senescence and abscission. Endogenous GAs were extracted, purified and analyzed by gas chromatography-selected ion monitoring with internal [(2)H(2)]GA standards. Across the sampled plant organs, GAs were generally highest in shoot tips and sequentially lower in basal stems, root tips, leaves and upper roots; GAs were thus abundant in rapidly growing tissues. Of the GAs measured, GA(1) tended to predominate, followed sequentially by GA(3), GA(8), GA(19), GA(20), GA(29) and GA(4). There was little relationship between GA concentration and growth allocation across the water table decline treatments, although GA(8) was consistently reduced in plants experiencing water table decline. Because GA(8) is the final gibberellin in the metabolic sequence, it might be useful for assessing historic patterns of GAs and growth rate. This study demonstrated changes in growth allocation in response to water table decline, but provided little evidence that endogenous GAs play a primary role in the regulation of root elongation in response to water table decline.     

Gas chromatographic screening method for T-2 toxin, diacetoxyscirpenol, deoxynivalenol, and related trichothecenes in feeds

A gas chromatographic method for screening trichothecene mycotoxins in feeds is described. Feed is extracted with acetonitrile-water, and the toxins are purified with charcoal-alumina-Celite, Florisil, and silica mini-columns. Deoxynivalenol (DON), nivalenol (NIV), diacetoxyscirpenol (DAS), T-2 toxin, and their fungal metabolites are hydrolyzed to their corresponding parent alcohols (DON, NIV, scirpentriol, or T-2 tetraol) by alkaline hydrolysis. After derivatization to their pentafluoropropionyl analogs, they are quantitated by capillary gas chromatography with electron capture detection. Identity can be confirmed and sensitivity can be increased by using negative chemical ionization mass spectrometry with no additional sample workup. Recoveries of DAS, DON, and T-2 toxin averaged, respectively, 80, 65, and 85% in corn; 84, 65, and 88% in soybeans; and 70, 57, and 96% in mixed feeds at concentrations ranging from 0.1 to 2.0 ppm. Recoveries of 15-monoacetoxyscirpenol (MAS), HT-2, NIV, and T-2 tetraol were 97, 97, 86, and 56%, respectively, in corn at a concentration of 0.25 ppm: A detection limit of 0.02 ppm in corn, soybeans, and mixed feeds, and 0.05 ppm in silages is estimated.     

安定桥滑坡的稳定性研究

安定桥滑坡位于四川省木里县博查公路约72公里处。滑坡地层为第四系松散堆积物,松散堆积物中有地下水存在。坡体内有软弱夹层。属于古滑坡体上的部分复活。坡体的滑动是由于古滑坡体内软弱夹层在上部岩体的自重应力及外部因素的综合作用下,产生的蠕动变形。两座拱桥的变形破裂是安定桥滑坡滑动变形所致。对两座拱桥实行加固和修复,有利于坡体的稳定和地方交通安全。