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991.
992.
Joel R. Coats Robert L. Metcalf Inder P. Kapoor 《Pesticide biochemistry and physiology》1974,4(2):201-211
Dianisylneopentane or 1,1-bis(p-methoxyphenyl)-2,2-dimethylpropane was metabolized largely by O-demethylation to form mono- and diphenol derivatives. Only a small percentage was degraded by α-hydroxylation and rearrangement. In the model ecosystem, dianisylneopentane reacted very similarly to methoxychlor, accumulating in fish to about the same extent and yielding a slightly higher ratio of polar to nonpolar metabolites. The neopentyl group proved to be approximately as stable in biological systems as the isosterically equivalent trichloromethyl group. 相似文献
993.
James R. Sanborn An-horng Lee Robert L. Metcalf 《Pesticide biochemistry and physiology》1974,4(1):67-76
In an effort to improve the generally unfavorable mouse/housefly toxicity ratio of most carbamate insecticides, potential selectophores (nitrile, carbamoyl oxime, carboxylic ester, or amide) were incorporated into a series of phenyl N-methylcarbamates. In addition to the insect and mouse toxicity determinations, the anticholinesterase activity of these compounds was determined for purified housefly head acetylcholinesterase and bovine erythrocyte acetylcholinesterase. The presence of these functional groups, in general, did not give enhanced selectivity ratios and, in one case, (o-N-methylcarbamoyloxyiminomethylphenyl N-methylcarbamate), a very unfavorable selectivity ratio of <0.03 was obtained. A mechanism implicating a Beckmann rearrangement is advanced to rationalize the high rodenticidal activity of this molecule. In general, the carbamates showed poor insecticidal activity when applied alone to the housefly, but, when the flies were pretreated with piperonyl butoxide, the compounds were quite toxic. Finally, an explanation is derived which seeks to justify the inability of these potential selectophores to improve the mouse/housefly toxidity ratio. 相似文献
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997.
Anna Catharina B Berge Elizabeth Thornburg John M Adaska Robert B Moeller Patricia C Blanchard 《Journal of veterinary diagnostic investigation》2008,20(4):497-500
The present study describes antimicrobial resistance patterns of Salmonella enterica subspecies enterica serovar Dublin (S. Dublin) in clinical submissions from calves and temporal and farm-type trends in antimicrobial resistance patterns of the isolates. A total of 300 isolates of S. Dublin were obtained from fecal or internal organs of calves fewer than 120 days of age originating from 84 dairies and 18 calf ranches from July 1998 to December 2002. The isolates were susceptibility tested to a panel of 10 antimicrobials using the disk diffusion assay. Temporal and farm-type trends in individual antimicrobial inhibition zone sizes were assessed and antimicrobial resistance patterns were described using cluster analysis. Isolates obtained from calf ranches compared with dairies exhibited decreased susceptibility to florfenicol, gentamicin, neomycin, sulfisoxazole, sulfamethoxazole/trimethoprim, and tetracycline. During the years 1998-2002, decreasing susceptibility was seen for ceftiofur, enrofloxacin, and sulfamethoxazole/trimethoprim. There were 20 different antimicrobial resistance patterns in the isolate set, indicating that S. Dublin has the ability to transfer and pick up resistance genes with relative ease. The trends seen in antimicrobial resistance in S. Dublin may likely be linked to antimicrobial drug use in young calves. 相似文献
998.
Western blot detection of PrP Sc in archived paraffin-embedded brainstem from scrapie-affected sheep
Robert A Kunkle Eric M Nicholson Semakaleng Lebepe-Mazur Dennis L Orcutt Megan L Srinivas Justin J Greenlee David P Alt Amir N Hamir 《Journal of veterinary diagnostic investigation》2008,20(4):522-526
Scrapie is a naturally occurring fatal neurodegenerative disease of adult sheep and goats, one of a group of mammalian diseases known as transmissible spongiform encephalopathies (TSE) or prion diseases. Immunoassays that identify disease-associated prion protein (PrP Sc) are integral to the diagnosis of scrapie and other prion diseases. Results obtained by either immunohistochemistry (IHC) or Western blot (WB) assay are generally adequate for the definitive diagnosis. Approved or accepted methods for WB diagnosis of TSEs requires the use of fresh or frozen nonfixed tissue samples, whereas formalin-fixed, paraffin-embedded tissue is required for the localization of PrP Sc by IHC. Because disparate processing methods are used for these accepted diagnostic techniques, separate tissue samples are collected from the same animal. Occasions arise in which there is either insufficient quantity of tissue available to complete analysis by both techniques or initial tissue processing is incompatible with one of the assays. Also, results between the assays may differ because of the vagaries of sampling, especially in case material that contains moderate-to-low levels of PrP Sc. The present article describes a method to conduct a WB assay from the same paraffin-embedded brainstem sample used for the IHC diagnosis of experimentally induced sheep scrapie. 相似文献
999.
Dorothea Besselmann Daniela Schaub Christian Wenker Jürg V?llm Nadia Robert Claude Schelling Hanspeter Steinmetz Marcus Clauss 《Journal of zoo and wildlife medicine》2008,39(1):86-91
Since 1956, when the Basle Zoo (Switzerland) initiated the breeding of lesser kudu (Tragelaphus imberbis), 43% of the lesser kudu juveniles died before reaching an age of 6 mo. In this study, the objective was to obtain the pathological findings, nutritional history, and family tree information in order to evaluate the influence of husbandry on juvenile mortality in these animals. The main cause of death was white muscle disease (WMD), diagnosed in 14 cases (26%) of the deceased juveniles. Although enclosure size had remained constant and animal accessibility to the public was constantly high, both herd size and juvenile mortality had increased from 1956-2004. The diet consumed by the whole group in 2004 had deficient levels of vitamin E and selenium. The increasing linear trend of the mortality rate since the 1960s was significant, and there was a significant correlation between herd size and overall juvenile mortality. In contrast, there was no correlation between herd size and the occurrence of juvenile mortality associated specifically with WMD. Other investigated factors (sex, inbreeding, and season) had no significant effect on overall mortality up to 6 mo of age or on mortality associated with WMD. These results characterize both a dietary and a husbandry problem, and are supported by a lack of similar juvenile mortality in another facility where the diet was supplemented with vitamin E, animal numbers were kept low, and the enclosure structure offered more retreat options for the animals. 相似文献
1000.
Conservationists, managers, and land planners are faced with the difficult task of balancing many issues regarding humans
impacts on natural systems. Many of these potential impacts arise from local-scale and landscape-scale changes, but such changes
often covary, which makes it difficult to isolate and compare independent effects arising from humans. We partition multi-scale
impacts on riparian forest bird distribution in 105 patches along approximately 500 km of the Madison and Missouri Rivers,
Montana, USA. To do so, we coupled environmental information from local (within-patch), patch, and landscape scales reflecting
potential human impacts from grazing, invasive plant species, habitat loss and fragmentation, and human development with the
distribution of 28 terrestrial breeding bird species in 2004 and 2005. Variation partitioning of the influence of different
spatial scales suggested that local-scale vegetation gradients explained more unique variation in bird distribution than did
information from patch and landscape scales. Partitioning potential human impacts revealed, however, that riparian habitat
loss and fragmentation at the patch and landscape scales explained more unique variation than did local disturbances or landscape-scale
development (i.e., building density in the surrounding landscape). When distribution was correlated with human disturbance,
local-scale disturbance had more consistent impacts than other scales, with species showing consistent negative correlations
with grazing but positive correlations with invasives. We conclude that while local vegetation structure best explains bird
distribution, managers concerned with ongoing human influences in this system need to focus more on mitigating the effects
of large-scale disturbances than on more local land use issues.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献