反应温度和时间对分光光度法测定乳粉中左旋肉碱的影响

采用分光光度法测定乳粉中左旋肉碱的含量,从温度和时间对反应的影响进行分析研究,确立了最佳反应温度为24℃,最佳反应时间为加入5,5＇-二硫双（2-硝基苯甲酸）和乙酰辅酶A反应6 min后归零,再加入乙酰肉碱转移酶反应6 min。此条件下该方法的回收率为98.3%～99.7%。     

柔性垫网接触式间歇干燥单板的中间试验

利用柔性垫网接触式间歇干燥单板的方法对毛白杨单板进行干燥。中间试验结果表明，此方法提高了单板干燥质量，生产工艺可行。     

SLHY0.125型混合机简化、优化设计

通过对SLHY0.125型混合机配套设备的简化、优化设计,使之能够适应中、小型饲料加工厂的现实生产条件。     

断裂力学在木材学中的应用

本文介绍断裂力学基本概念及其在木材科学中的应用，应力强度因子的测试方法和计算方法以及国内外木材断裂力学的研究现状。     

Analysis of the influence of land finance on haze pollution: An empirical study based on 269 prefecture-level cities in China

Under the system of political centralization and economic decentralization, the expanding scale of land finance and the increasingly severe environmental pressure have jointly become crucial features of China's urban development. Therefore, it is of great practical significance to study the intrinsic mechanism of land finance on haze pollution for China's economy to achieve kinetic energy transformation and green development. This paper empirically analyzes the impact of land finance on haze pollution using a dynamic spatial Durbin model based on panel data of 269 prefecture-level cities in China from 2004 to 2017. The statistical results show that haze pollution has a significant “snowball effect” and space spillover effect. Land finance has a significant positive effect on haze pollution. Land transfer both by agreement and by bid invitation, auction, and listing have significant positive effect on haze pollution. However, the promoting effect of land transfer by agreement on haze pollution is significantly higher than that of land sale by bid invitation, auction, and listing. Furthermore, regional heterogeneity implies that for cities in the eastern region, land finance is conducive to alleviating haze pollution. In contrast, for cities in the central and western regions, land finance significantly promotes haze pollution.     

Resveratrol Downregulates Cyp2e1 and Attenuates Chemically Induced Hepatocarcinogenesis in SD Rats

Cyp2e1 plays an important role in chemically induced hepatocarcinogenesis. Resveratrol (REV) is known to prevent diethylnitrosamine (DEN)-induced hepatocarcinogenesis, but its effects on this process induced by DEN and 2-acetylaminofluorene (2-AAF) and the role of Cyp2e1 remain unclear. In this study, glutathione S-transferase placental form (GST-P)-positive foci were used as a marker of hepatocarcinogenesis. REV or diallyl disulfide (DADS, an inhibitor of Cyp2e1) significantly reduced both the area and number of GST-P-positive foci induced by DEN and 2-AAF. Treatment with REV or DADS also markedly decreased the expression of Cyp2e1 in the rat liver. By immunohistochemical staining of serial liver sections, we found that the expression of Cyp2e1 in GST-P-positive foci showed three distinct patterns: decreased in GST-P foci, increased in GST-P foci when compared with surrounding liver tissue and mixed type. The number of GST-P foci with increased Cyp2e1 expression was greater than the number of GST-P foci with decreased Cyp2e1. Protein levels of GST-P and Cyp2e1 were also higher in foci compared with surrounding liver tissue. REV or DADS significantly reduced the expression of GST-P and Cyp2e1 in both foci and surrounding liver tissue. Taken together, these results suggested that REV has a significant inhibitory effect on chemically induced hepatocarcinogenesis, which may be attributed to downregulation of Cyp2e1.     

耐高温植酸酶用于颗粒饲料对肉鸡生产性能及钙、磷利用率的影响

试验旨在研究肉仔鸡低磷日粮颗粒饲料中添加耐高温植酸酶对肉鸡生产性能及钙、磷利用率的影响。选取1日龄AA+肉仔鸡,随机分为6个处理,分别饲喂处理1(正对照,正常磷水平)、处理2(负对照,低于正常磷水平)、处理3(负对照+500U植酸酶)、处理4(高磷替代+500U植酸酶)、处理5(负对照+1000U植酸酶)和处理6(高磷替代+1000U植酸酶)试验日粮。试验结果表明:在制粒饲料中添加耐高温植酸酶是可行的。在相同有效磷水平下,随着植酸酶添加水平的提高,出栏体重有增加的趋势;在相同植酸酶添加量的条件下,随着有效磷替代水平的提高,出栏体重有降低的趋势。添加植酸酶有增加胫骨灰分和磷含量的趋势。在正常替代水平下,植酸酶的添加量以500～1000U/kg为宜。在本试验条件下,日粮添加500～1000U/kg植酸酶的情况下,进行磷酸氢钙的高水平替代也是可行的。     

Cloning and bioinformatics analysis of cDNA encoding cattle Smad4 gene

The cDNA of cattle Smad4 gene was cloned by RT-PCR, 3′ RACE and 5’t RACE and got a 3503-bp full-long cDNA sequence. The cloned cattle Smad4 cDNA sequence had been send to GenBank and got an accession number: DQ494856. Cattle Smad4 gene consists of 12 exons and codes 553 amino acids. Cattle Smad4 cDNA shares 99%, 96%, 95%, 91% and 91% similarity in nucleic acid sequences, and 99%, 98%, 98%, 99% and 98% similarity in amino acid sequences with sheep, pig, human, rat and mouse, respectively. Smad4 cDNA was found in the testes, pancreas, liver, small intestine, ovary, lymph, cardiac muscle, skeleton muscle and thymus gland, which indicated that Smad4 was broadly expressed in cattle. __________ Translated from Acta Veterinaria et Zootechnica Sinica, 2007, 38(4): 321–325 [译自: 畜牧兽医学报]     

依靠科技创新推进农业产业化的战略与策略Ⅱ. 重点任务之构建产业技术链

推进农业产业化的首要任务是攻克制约产业发展的关键共性技术,构建起现代农业产业技术链。文章在认真梳理现代农业产业的基础上,针对产业链重要环节需求,提出了七大共性关键技术,即良种繁育技术—标准化种养殖技术—精深加工技术—副产物综合利用技术—废弃物循环利用技术—机械化生产技术—节水农业技术。只有构建起技术链,才能支撑延长产业链,这是现代农业产业化发展的必然规律。