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31.
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(1) Flocculation of cephalin-cholesterol emulsions by blood serum is markedly influenced by the amount of light to which the serum-saline-antigen suspensions are exposed. Protection from bright light, natural and artificial, has eliminated many falsely positive reactions. (2) Other factors that appear to influence the cephalin flocculation procedure have been briefly mentioned. Misses Dorothy Feinberg, Arvilla Howley and Mary Lanning contributed helpful technical assistance. 相似文献
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Kleiner R 《Science (New York, N.Y.)》2007,318(5854):1254-1255
36.
Neurotransmitter release is triggered by calcium ions and depends critically on the correct function of three types of SNARE [soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor] proteins. With use of the large calyx of Held presynaptic terminal from rats, we found that cleavage of different SNARE proteins by clostridial neurotoxins caused distinct kinetic changes in neurotransmitter release. When elevating calcium ion concentration directly at the presynaptic terminal with the use of caged calcium, cleavage of SNAP-25 by botulinum toxin A (BoNT/A) produced a strong reduction in the calcium sensitivity for release, whereas cleavage of syntaxin using BoNT/C1 and synaptobrevin using tetanus toxin (TeNT) produced an all-or-nothing block without changing the kinetics of remaining vesicles. When stimulating release by calcium influx through channels, a difference between BoNT/C1 and TeNT emerged, which suggests that cleavage of synaptobrevin modifies the coupling between channels and release-competent vesicles. 相似文献
37.
Anthony RM Nimmerjahn F Ashline DJ Reinhold VN Paulson JC Ravetch JV 《Science (New York, N.Y.)》2008,320(5874):373-376
It is well established that high doses of monomeric immunoglobulin G (IgG) purified from pooled human plasma [intravenous immunoglobulin (IVIG)] confer anti-inflammatory activity in a variety of autoimmune settings. However, exactly how those effects are mediated is not clear because of the heterogeneity of IVIG. Recent studies have demonstrated that the anti-inflammatory activity of IgG is completely dependent on sialylation of the N-linked glycan of the IgG Fc fragment. Here we determine the precise glycan requirements for this anti-inflammatory activity, allowing us to engineer an appropriate IgG1 Fc fragment, and thus generate a fully recombinant, sialylated IgG1 Fc with greatly enhanced potency. This therapeutic molecule precisely defines the biologically active component of IVIG and helps guide development of an IVIG replacement with improved activity and availability. 相似文献
38.
Reichel M Carle R Sruamsiri P Neidhart S 《Journal of agricultural and food chemistry》2011,59(8):3924-3939
Polyphenols, chlorophylls, and carotenoids were characterized by HPLC-DAD-MS(n) in the pericarp of unripe to over-ripe 'Hong Huey' and 'Chacapat' litchi fruit at harvest and during subsequent storage (5 °C, 90% RH, 21 days). (-)-Epicatechin and A-type procyanidins always predominated quantitatively. Besides these ortho-diphenolic compounds, minor novel litchi flavonoids included monohydroxylated structures. Chlorophyll degradation by 73-92% and 7-38-fold anthocyanin accumulation affected pericarp color throughout the last 15-20 days of on-tree maturation. Postharvest, anthocyanins and (-)-epicatechin largely degraded within the first 3 days, accompanied by severe pericarp browning. Without packaging of the fruit, desiccation initially accelerated polyphenol oxidase-induced oxidation of (-)-epicatechin, but then hindered its further progress. Constant levels of the monohydroxylated (epi)afzelechin indicated no involvement of peroxidase. Acting as antioxidants, anthocyanins retarded (-)-epicatechin degradation. Hence, pinkish-red fruit with a molar ratio of cyanidin 3-O-rutinoside to (-)-epicatechin of >3:100 retained flavonoids best. However, brown polymers masked remaining red pigments. 相似文献
39.
Respiratory function and pulmonary lesions in pigs infected with porcine reproductive and respiratory syndrome virus 总被引:1,自引:0,他引:1
Judith Wagner Annette Kneucker Elisabeth Liebler-Tenorio Vicky Fachinger Melanie Glaser Stefan Pesch Michael P. Murtaugh Petra Reinhold 《Veterinary journal (London, England : 1997)》2011,187(3):310-319
Pulmonary dysfunction was evaluated in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV, isolate VR-2332) and compared to clinical and pathological findings. Infected pigs developed fever, reduced appetite, respiratory distress and dullness at 9 days post-inoculation (dpi). Non-invasive pulmonary function tests using impulse oscillometry and rebreathing of test gases (He, CO) revealed peripheral airway obstruction, reduced lung compliance and reduced lung CO-transfer factor. PRRSV-induced pulmonary dysfunction was most marked at 9–18 dpi and was accompanied by a significantly increased respiratory rate and decreased tidal volume. Expiration was affected more than inspiration. On histopathological examination, multifocal areas of interstitial pneumonia (more severe and extensive at 10 dpi than 21 dpi) were identified as a possible structural basis for reduced lung compliance and gas exchange disturbances. 相似文献
40.
Soil systematics and classification systems Part I: Fundamentals Soil‐ordering systems are primarily based and developed on one of two underlying principles: They are either categorized according to soil‐forming processes, or the formation of categories develops by chosen parameters. This perspective has already been established in the literature, though it is often confusing as many terms are defined and applied differently. In this contribution, the various definitions of systematics, classification, taxonomy, and identification will be clearly differentiated and summarized. The core of our work is to clearly define and contrast three terms: systematics, classification, and identification. Systematics is the fundamental scientific and deductive ordering of objects into systematic units. The purpose of this approach is to organize the entire spectrum of knowledge within a discipline into a transparent and manageable form. Classification, in direct contrast to systematics, is goal‐oriented and an inductive ordering of objects. Thus, the ordering scheme consists of classes which are clearly parameterized. Identification is the ordering of new objects into an already existing systematics or classification system. Close attention is paid to both the differences and the similarities between a systematics and a classification system, especially pertaining to their practical applications. The identification requires that the category‐forming characteristics can be measured (e.g., for soil systematics, these are the soil‐forming processes and factors). Currently, it is unfortunately not feasible to objectively quantify most soil‐forming processes. Thus, most attempts at categorizing soils by systematics are hypothetical and highly subjective in nature. The resulting identification derived from the soil systematics approach is open to questions and contestable, since a graded measuring system does not yet exist to verify these determinations. In contrast, a soil‐classification system does allow an objective soil‐profile identification, although such systems are conceived pragmatically and designed for a practical purpose (e.g., not scientifically based on process intensities). Unfortunately, such a classification system cannot be applied as a universal scientific categorization system due to this method of conception. Both categorization approaches are required in soil science in order to satisfy both the practical and the scientific aspects of the field. However, substantial research must be done to complete and verify systematics. The only viable short‐term solution is through the development of a graded classification system where the categories of the system are directly derived from the current systematics approach. In the long run both the exact investigation and the detailed modeling of the soil‐forming processes are inevitable. 相似文献