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31.
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The effect of the type and concentration of plant growth regulators and sub-culturing on somaclonal variation were studied in Cavendish banana cv. ‘Zelig’ obtained from African Biotechnologies Ltd., South Africa. In vitro grown plants at the fourth multiplication cycle were used for the investigation. Auxins (IAA, IBA and NAA) and cytokinins (BA and TDZ) were used to multiply shoots for 10 generations. Bands generated through RAPD-PCR were scored according to whether they were present (1) or absent (0) to determine the extent of somaclonal variation. Results were then analyzed using cluster analysis. The relationship between multiplication rate and somaclonal variation was assessed using correlation analysis. Results indicated that treatments with higher multiplication rates produced more variants; sometimes as high as 72%. Dwarf off-types accounted for 88% of the variation. A dwarf-specific band, about 1500 kb in size, was amplified by the primer OPC-15. The band appeared consistently in normal plants but was absent in all dwarf plants. 相似文献
33.
In a naturally regenerated beech (Fagus sylvatica L.) standin northeastern France, five regeneration patches were selectedwith different degrees of canopy closure; the percentage ofabove-canopy light (PACL) ranged between 5 and 45 per cent.The canopy was removed by a storm 2 years later. In order toanalyse the immediate and delayed effects of PACL on seedlingdiameter and height growth, the effects of the light conditionsprevailing before canopy opening on seedling growth measuredbefore and after canopy opening were tested. The effects oflocal density were examined using neighbourhood analysis. Fourdifferent competition indices (CIs) and neighbourhood radiiranging from 10 to 200 cm were tested. Models including PACLand CI accounted for between 56 and 64 per cent of the variationin individual seedling annual diameter or height growth. Localdensity had a strong negative influence on diameter growth anda much smaller influence on height growth. PACL was positivelycorrelated with diameter and height growth before canopy opening.Delayed effects of PACL on height growth were observed justafter canopy opening, but disappeared after 2 years. No delayedeffects of PACL on diameter growth after canopy opening wereobserved. 相似文献
34.
We examined morphological and physiological responses of beech (Fagus sylvatica L.) seedlings to grass-induced below ground competition in full-light conditions. Two-year-old beech seedlings were grown during two growing seasons in 160-l containers in bare soil or with a mixture of five grass species widely represented in semi-natural meadows of central France. At the end of the second growing season, beech seedlings in the presence of grass showed significant reductions in diameter and height growth, annual shoot elongation, and stem, root and leaf biomass, but an increase in root to shoot biomass ratio. Grasses greatly reduced soil water availability, which was positively correlated with daily seedling diameter increment. Beech seedlings seemed to respond to water deficit by anticipating stomatal closure. There was evidence of competition for nitrogen (N) by grasses, but its effect on seedling development could not be separated from that of competition for water. By labeling the plants with 15N, we showed that beech seedlings absorbed little N when grasses were present, whereas grasses took up more than 97% of the total N absorbed in the container. We conclude that, even if beech seedlings display morphological and physiological adaptation to below ground competition, their development in full-light conditions may be strongly restricted by competition from grass species. 相似文献
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Pascal C Poncet-Legrand C Imberty A Gautier C Sarni-Manchado P Cheynier V Vernhet A 《Journal of agricultural and food chemistry》2007,55(12):4895-4901
Interactions between salivary proline-rich proteins and tannins are involved in astringency, which is one of the most important organoleptic sensations perceived when drinking wine or tea. This work aimed to study interactions between a recombinant human salivary proline-rich protein, IB-5, and a flavan-3-ol monomer, epigallocatechin gallate (EGCG). IB-5 presented the characteristics of natively unfolded proteins. Interactions were studied by dynamic light scattering, isothermal titration microcalorimetry, and circular dichroism. The interaction mechanism was dependent on protein concentration. At low concentrations, a three-stage mechanism was evidenced. Saturation of the interaction sites (first stage) was followed by protein aggregation into metastable colloids at higher EGCG/protein ratios (second stage). Further increasing this ratio led to haze formation (third stage). At low ratios, a disorder-to-order transition of IB-5 structure upon binding was evidenced. At high protein concentrations, direct bridging between proteins and EGCG was observed, resulting in significantly lower aggregation and turbidity thresholds. 相似文献
37.
Derero Abayneh Coe Richard Muthuri Catherine Hadgu Kiros M. Sinclair Fergus 《Agroforestry Systems》2021,95(7):1309-1326
Agroforestry Systems - Increasing tree cover and managing trees better on farms in Ethiopia supports livelihoods and the environment but most tree-planting schemes promote only a few species. This... 相似文献
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Two non-invasive blood pressure (NIBP) devices (oscillometry and Doppler) were compared to invasive blood pressure using a Bland–Altman analysis, in anesthetized and conscious dogs. When considering the systolic arterial pressure only during general anesthesia, both NIBP devices slightly underestimated the systolic arterial blood pressure however the precision and the limits of agreement for the Doppler were of a greater magnitude. This indicates a worse clinical performance by the Doppler. The performance of both NIBP devices deteriorated as measured in conscious animals. In general, for the oscillometric device, determination of invasive diastolic and mean arterial pressures was better than the invasive systolic arterial pressure. Overall, the oscillometric device satisfied more of the criteria set by the American College of Veterinary Internal Medicine consensus statement. Based upon these results, the oscillometric device is more reliable than the Doppler in the determination of blood pressure in healthy medium to large breed dogs. 相似文献
40.
Heli K Hyyti?inen Anna K Mykk?nen Anna K Hielm-Bj?rkman Narelle C Stubbs Catherine M McGowan 《Acta veterinaria Scandinavica》2014,56(1):8