A study on the effect of thiotepa on mice spermatogenesis using light and electronic microscope

The present study was designed to explore the spermatogenic effect of intraperitoneal administration of thiotepa in mice. It is known the chemotherapy could affect testicular function and result in infertility. There are few reports on the side effect of thiotepa on spermatogenesis when are used as anticancer drug. Therefore, in the present study the effect of thiotepa on spermatogenesis was evaluated by electron microscopy. A total of 20 balb/c mice were used in this study. They were divided into control and thiotepa treated groups. Thiotepa was administrated as 2.5 mg kg(-1) for 5 days. The mice were sacrificed after 5 weeks and testicular specimens were removed, fixed in boueins fixative and 2.5% Glutaraldehide then prepared for light and electron microscopic study. Light microscopy showed that the thickness of germinal epithelium was decreased in thiotepa treated group and many seminiferous tubules have lost germinal cells. Electron microscopy revealed that in experimental group several intercellular spaces appeared between spermatogonial and sertoli cells. The basement membrane became irregular and intercellular vacuoles were present in sertoli and spermatogonial cells. Nuclear chromatin was condensed and there were several vacuolated mitochondria. It was indicated that thiotepa affected testicular germinal epithelium by both cytotoxic effect and induction of apoptosis.     

CHARACTERIZATION OF PHYSIOLOGIC 18F‐FDG UPTAKE WITH PET‐CT IN DOGS

We evaluated the whole body distribution of 2‐deoxy‐2‐[18F]fluoro‐d ‐glucose (¹⁸F‐FDG) in seven beagle dogs using positron emission tomography/computed tomography. The mean and maximum standard uptake values (SUV) for various tissues were computed. The SUV of the aortic blood pool was 0.65±0.19. Moderate uptake was present in brain (3.40±1.01). Mild uptake was present in orbital muscles, soft palate, laryngeal and pharyngeal region, mandibular salivary gland, myocardium, liver, pancreas, kidney, and intestine. ¹⁸F‐FDG uptake would be normally higher in these tissues because of normal physiologic activity. Mean and maximum SUV values of the eye, skeletal muscle, bone tissue, spleen, adrenal gland, stomach, tongue, gall bladder, and lung were similar to or lower than that of the aortic blood pool. These data provide a normal baseline for comparing pathologic ¹⁸F‐FDG uptake.     

Antibacterial,UV protective and ammonia sensing functionalized polyester fabric through <Emphasis Type="Italic">in situ</Emphasis> synthesis of cuprous oxide nanoparticles

In this research, a facile and cost effective method is presented for in-situ synthesis of cuprous oxide nanoparticles on polyester fabric along with surface modification by using one pot wet chemical method at boil. Copper sulfate (CuSO₄), sodium hypophosphite (SHP) and polyvinylpyrrolidone (PVP) were used as precursor, reducing agent and stabilizer, respectively. Further, monoethanolamine (MEA) was used as pH adjustment and also modifier of polyester surface introduced amide and hydroxyl functional groups on the fabric. The images of FESEM, mapping, patterns of EDX, UV-visible absorbance spectrum, FTIR analysis and X-ray diffraction pattern confirmed the successful processing. The treated samples showed excellent antibacterial properties (100 %) toward both Staphylococcus aureus and Escherichia coli pathogen bacteria. Also, the results of cytotoxicity test proved no harmful effects on the human dermal fibroblasts for the treated sample with the lower concentration of the materials having white color with good antibacterial activities. The treated samples also indicated very good UV protection properties as well as improved wettability and mechanical properties. They are also sensitive to ammonia through immediate color change when contacted with ammonia solution. The above mentioned processing method can be used for production of polyester fabric with multifunctional properties for using in the various textile industries.     

Molecular identification of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus in nasal swabs samples from horses suffering respiratory infections in Iran

The objective of this study was to evaluate the existence of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus as probable agents associated with naturally occurring infection of the equine upper respiratory disease in Mashhad area. Nasal swabs samples from thirty horses with upper respiratory tract infections were collected. The bacteria isolated and identified were Streptococcus equi subsp. equi (1 isolate), Streptococcus equi subsp. zooepidemicus (25 isolates), Pasteurella sp. (11 isolates), Staphylococcus sp. (17 isolates), Bacillus sp. (4 isolates), Pseudomonas sp. (4 isolates), Proteus sp. (1 isolate), Neisseria sp. (1 isolate) and E. coli (1 isolate). All 25 isolates of Streptococcus equi subsp. zooepidemicus and the isolate of Streptococcus equi subsp. equi were characterized by biochemical tests and molecular techniques. For molecular identification of the subspecies S. equi and S. zooepidemicus two genomic region SeM and sodA were amplified. This study is the first report of molecular identification of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus in Iran.     

Irrigation effects on diameter growth of 2-year-old Paulownia tomentosa saplings

Species of Paulownia are fast-growing trees that are planted in many countries in green spaces and agroforestry systems. We studied the influence of irrigation on the diameter growth of 2-year-old Paulownia tomentosa saplings planted in 3 m 9 3 m grids at Nazlou Campus of Urmia University, Iran. Completely randomized experimental design was used with four irrigation amount, viz.10, 15, 20 and 25 L, and three irrigation intervals, viz. 3-and 6-days, in 8 replications. During the growth season, we measured collar diameters of all saplings at the end of each month. Diameter growth declined with reduced irrigation amount and increased irrigation interval. The lowest average diameter growth(9 mm) was recorded for 10 L water at 6-day intervals and maximum average diameter growth(28.7 mm) was recorded for 25 L volume at 3-day intervals. The latter treatment resulted in significantly greater diameter growth than any other treatment(except20 L at 3-day intervals). More than 80 % of diameter growth was achieved in the first 3 months. Therefore, it is essential to irrigate with 25 L of water at 3-day intervals during the first three growth months to achieve maximum diameter growth for 2-year-old P. tomentosa saplings.     

Evaluation of the effects induced by dietary diphenyl diselenide on common carp Cyprinus carpio

Several diets employed in aquaculture are enriched with selenium (Se), as it is a fundamental element to aquatic vertebrates. Diphenyl diselenide [(PhSe)₂], which is a synthetic organoselenium compound, has been considered a potential antioxidant agent in different experimental models. Thus, the aim of this study was to evaluate the effects of dietary diphenyl diselenide at concentrations of 1.5, 3.0, and 5.0 mg/kg for 60 days and to determine its optimal supplemental level for carp, Cyprinus carpio. Neither growth retardation nor hepatoxicity was induced by the inclusion of diphenyl diselenide at concentrations ranging from 1.5 to 5.0 mg/kg. In addition, the inclusion of 3.0 mg/kg of diphenyl diselenide stimulated the weight and length of the carp. The supplementation with 1.5 and 3.0 mg/kg of diphenyl diselenide did not produce oxidative damage in the tissues, verified by peroxidation lipid and protein carbonyl assays. However, at 5.0 mg/kg, it caused an increase of the lipid peroxidation in the liver, brain, and muscle, and inhibited the cerebral acetylcholinesterase activity. An increase of the hepatic superoxide dismutase activity and non-protein thiols content in all tissues and ascorbic acid in the liver, gills, and brain was verified in carp fed with the diet containing 3.0 mg/kg of diphenyl diselenide. This diet had advantageous effects for the fish used in experiments. Therefore, this compound could be considered a beneficial dietary supplement for carp nutrition.     

Effect of Winter Planting Date on Oil Yield and Fatty Acids of New Spring Canola (Brassica napus L.) Cultivars Under Foliar Zinc Spray

Canola (Brassica napus L.) oil yield and fatty acid composition are the function of genotype, climate conditions, morphology, and physiology as well as crop management. In this study, the reaction of different canola cultivars—in terms of seed oil content and fatty acids—to different planting dates and foliar zinc application during two experimental years (2016–2017) was investigated in the field in arid and semi-arid regions of Karaj, Iran. The experiment was performed as a factorial split plot in a randomized complete-block design with three replications. Three sowing dates (February 9, 19, 29) and two foliar zinc applications (non-application as control and zinc application; spraying in the stem elongation stage) were factorially randomized to main plots, and canola cultivars (Sarigol, Dalgan, Salsa, and Solar) were allocated to sub-plots. The results indicated that the effect of year, planting date, zinc application, cultivar, and the interaction effect of planting date and cultivar on quality traits (glucosinolate and fatty acids) and seed yield were significant (p?<?0.01). The delay in winter planting of spring canola cultivars caused a significant reduction in seed yield, oil yield, and the content of oleic, linoleic, and palmitic fatty acids and increased the content of linolenic, erucic, stearic, arachidic, and behenic fatty acids and glucosinolate. The highest seed yield (4732.2?kg/ha), oil yield (2066?kg/ha), and oleic acid content (66.52%) were observed in the Dalgan cultivar in the zinc application treatment with normal planting date (February 9). The Salsa cultivar had the highest content of erucic acid (0.46%) and glucosinolate (17.3?μmol/g meal) in the control treatment with the last planting date. Because the quality of edible canola oil depends on the increase in unsaturated fatty acids, particularly oleic and linoleic acids, and the reduction of erucic acid and glucosinolate, planting Dalgan cultivar with zinc application at the normal planting date is recommended for the study area and similar areas.

     

西南岩溶区粉垄耕作和免耕方式下甘蔗地土壤优先流特征

以西南岩溶区的典型农地甘蔗地为研究对象,采用野外染色示踪技术,利用形态学解析手段,并结合土壤水分分层评价方法,对粉垄耕作和免耕方式下甘蔗地土壤优先流特征进行研究。结果表明:在相同外部供水条件下,粉垄耕作甘蔗地水分下渗湿润锋迹线较免耕甘蔗地平缓,呈均匀整体形式下渗,平均下渗深度是免耕甘蔗地的0.95倍,其优先流染色形态相对于免耕甘蔗地的枝状分化状态,多以零散团块状分化形式为主;在相同外部条件下,免耕甘蔗地最先发生优先流现象,优先流的发生速度是粉垄耕作甘蔗地的1.45倍,平均优先流比达88.61%,大于粉垄耕作甘蔗地(82.89%),二者之间差异显著(P0.05);沿土壤深度的增加,优先流在粉垄耕作甘蔗地土壤空间中的发展变化程度表现为次活跃—活跃—次活跃—相对稳定—次活跃,而免耕甘蔗地表现为次活跃—活跃—次活跃—相对稳定,免耕甘蔗地的优先流空间发展变化程度较高,主要发生在5—35cm土层范围内,相比粉垄耕作甘蔗地10—30cm土层深。粉垄耕作方式与免耕方式相比,降低了岩溶区甘蔗地土壤优先流的发生与发展,在一定程度上提高了土壤的保肥蓄水能力。