全文获取类型
收费全文 | 2915篇 |
免费 | 239篇 |
国内免费 | 5篇 |
专业分类
林业 | 277篇 |
农学 | 109篇 |
基础科学 | 7篇 |
664篇 | |
综合类 | 119篇 |
农作物 | 140篇 |
水产渔业 | 358篇 |
畜牧兽医 | 1153篇 |
园艺 | 59篇 |
植物保护 | 273篇 |
出版年
2024年 | 4篇 |
2023年 | 35篇 |
2022年 | 43篇 |
2021年 | 80篇 |
2020年 | 87篇 |
2019年 | 79篇 |
2018年 | 162篇 |
2017年 | 145篇 |
2016年 | 167篇 |
2015年 | 102篇 |
2014年 | 145篇 |
2013年 | 211篇 |
2012年 | 250篇 |
2011年 | 222篇 |
2010年 | 116篇 |
2009年 | 109篇 |
2008年 | 153篇 |
2007年 | 163篇 |
2006年 | 143篇 |
2005年 | 115篇 |
2004年 | 105篇 |
2003年 | 92篇 |
2002年 | 95篇 |
2001年 | 55篇 |
2000年 | 27篇 |
1999年 | 36篇 |
1998年 | 13篇 |
1997年 | 16篇 |
1996年 | 6篇 |
1995年 | 17篇 |
1994年 | 9篇 |
1993年 | 5篇 |
1992年 | 11篇 |
1991年 | 12篇 |
1990年 | 9篇 |
1989年 | 6篇 |
1988年 | 16篇 |
1987年 | 5篇 |
1986年 | 9篇 |
1985年 | 5篇 |
1984年 | 6篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1980年 | 8篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1973年 | 8篇 |
1969年 | 3篇 |
排序方式: 共有3159条查询结果,搜索用时 359 毫秒
111.
Pere M. Parés-Casanova Raúl Pérezgrovas Garza 《Tropical animal health and production》2014,46(5):777-781
Forty-nine sheep belonged to the Andean Altiplano region (“Altiplano”) and 30 in the lowland regions of Bolivia (“Valle”), aged 1 to 4 years, were wool sampled to determine the extent of difference between these local breeds. Fibre length and the percentage of each type of fibre (long-thick, short-thin and kemp), yield and fibre diameter were measured. There was a highly significant difference between the two sheep populations that were not clearly separated in the first two principal component of a principal components analysis (PC); the first PC explained 67.1 % and the second PC explained 26.6 % of the total variation. The variables that contributed most to the separation of the sheep populations were the percentage of long-thick and short-thin fibres in the first PC and yield in the second PC. A discriminant analysis, which was used to classify individuals with respect to their breeding, achieved an accurate classification rate of 84.2 %. Thus, the Altiplano and Valle sheep must be viewed as two closely peripatric breeds rather than different “ecotypes”, as more than 80 % could be correctly assigned to one of the breeds; however, the differences are based on composition of long-thick and short-thin fibres and yield after alcohol scouring. 相似文献
112.
Steffan Edward Octávio Oliveira Cíntia Carol de Melo Costa João Batista Freire de Souza Jr João Paulo Araújo Fernandes de Queiroz Alex Sandro Campos Maia Leonardo Lelis de Macedo Costa 《Tropical animal health and production》2014,46(8):1413-1417
The amount of short-wave solar radiation willingly tolerated by lactating Holstein cows on pasture was determined in an equatorial semi-arid environment. The study was carried out on a dairy farm located in Limoeiro do Norte, CE, northeastern Brazil. The observed behaviours were as follows: grazing, under the sun, under the shade, standing, lying, ruminating, idling and wallowing in the water. The behaviours were recorded using instantaneous scan sampling at regular intervals of 15 min from 0600 to 1800 hours over 5 days. On all sampling days, the meteorological variables, including local short-wave solar radiation (R S-W, W m?2), were recorded. The R S-W data were divided into five levels. The sun exposure was more frequent under low (100 %) and moderately low (97 %) levels, when R S-W remained below 500 W m?2. The grazing was more intense under low (100 %) and moderately low (93 %) levels. Above 500 W m?2, the grazing time significantly decreased (11 %). The cows avoided grazing under high (0 %) and very high (0 %) levels, when R S-W exceeded 700 W m?2. The ruminating behaviour was more frequent under high (33 %) and very high (37 %) levels, in which the highest averages of R S-W were recorded (815 and 958 W m?2, respectively). The standing posture was more frequent under low (100 %) and moderately low (97 %) levels. Therefore, the critical R S-W level that motivates cows to stop grazing and seek shade was in the interval between 500 and 700 W m?2. 相似文献
113.
Marconni Victor da Costa Lana Noemi Rovaris Gardinali Raquel Aparecida Sales da Cruz Letícya Lerner Lopes Gustavo Sousa Silva João Garcia Caramori Júnior Anderson Castro Soares de Oliveira Marcos de Almeida Souza Edson Moleta Colodel Amauri Alcindo Alfieri Caroline Argenta Pescador 《Tropical animal health and production》2014,46(2):399-404
The objectives of this study were to (1) investigate the occurrence of hepatitis E virus (HEV) in pigs from large-scale and family-scale farms, (2) genetically characterize the strains isolated, and (3) study the pathogenesis of swine HEV infection via immunohistochemistry. A total of 50 pigs from 10 farms in Mato Grosso State, Brazil were divided according to type of production system into either large-scale farms (n?=?5) or family-scale farms (n?=?5). Samples of liver, gallbladder, small and large intestines, bile, and feces from the pigs were analyzed by nested PCR with primers targeting the ORF2 region of HEV and by immunohistochemistry. Of the eight HEV-positive samples from pigs of family-scale farms, phylogenetic analysis revealed that seven of the swine HEV isolates clustered with subtype 3b of genotype 3 and one isolate was categorized with subtype 3 f. The HEV antigen was detected mainly in the small intestine samples from family-scale farms, suggesting an early stage HEV infection. HEV was not detected in the samples of pigs from large-scale farms, reinforcing the need for additional studies to evaluate the risk of transmission of HEV to humans from pigs from family-scale farms in Mato Grosso State. 相似文献
114.
Manoel Eduardo da Silva Fabio Ribeiro Braga Luana Alcântara Borges Jair Mendes de Oliveira Walter dos Santos Lima Marcos Pezzi Guimarães Jackson Victor de Araújo 《Veterinary research communications》2014,38(2):101-106
Brazil has a herd of 212 million cattle and 171 million hectares of pastures that produce approximately 96 % of Brazilian beef. The Brazilian production system enables animal infection by endoparasites, which are considered one of the main obstacles for the development of this industry and are responsible for considerable economic losses. The control of parasitic diseases is performed via the administration of antiparasitic drugs, but they leave residues of the products in the treated animal, affect non-target organisms and select resistant strains of the parasites. The species D. flagrans and M. thaumasium are promising and sustainable alternatives for controlling gastrointestinal helminths of ruminants and other herbivores. In this study, we evaluated the efficacy of isolates of these species, formulated in a sodium alginate matrix and administered twice a week, to reduce the number of environmental infective larvae of gastrointestinal nematodes that affect prepubescent zebu females. The treated animals presented fewer eggs and a lower number of infective larvae per gram of faeces (p?<?0.05). The pastures occupied by treated animals showed a statistically significant reduction (p?<?0.05) of the number of L3 and, furthermore, the genera Cooperia sp., Haemonchus sp., and Oesophagostomum sp. were the most prevalent. The average weight of the animals did not differ statistically (p?>?0.05) among the treated and control groups. The use of sodium alginate pellets as vehicle for delivery of the fungus mycelia D. flagrans (isolate AC001) and M. thaumasium (isolate NF34A) proved effective in controlling trichostrongylids in prepubescent cows bred in the semi-arid region, with an effective reduction in the number of infective larvae in the pastures. 相似文献
115.
Higher feeding diets effects on age and liveweight gain at puberty in crossbred Nelore × Hereford heifers 总被引:1,自引:1,他引:0
Júlio Otávio Jardim Barcellos Gabriel Ribas Pereira Eduardo Antunes Dias Concepta McManus Leonardo Canellas Mari Lourdes Bernardi Adriana Tarouco Ênio Rosa Prates 《Tropical animal health and production》2014,46(6):953-960
This study was conducted to determine the age and liveweight at puberty of 120 crossbred beef heifers submitted to four diets to achieve predetermined weight gains (kg/day): 0.5 (G500; n?=?32), 0.75 (G750; n?=?32), 1.0 (G1000; n?=?29), and 1.25 (G1250; n?=?27). Animals were classified depending on their level of crossing between Nelore (N) and Hereford (H): 25%N-75%H, 37.5%N-63.5%H, 43.7%N-56.7%H, 50%N-50%H, and 75%N-25%H. Reproductive evaluation was performed at the beginning and at the end of the experimental period by ultrasonography and hormone analysis. The average age and liveweight at puberty were 388.0?±?1.9 days and 331.4?±?1.3 kg, respectively. Animals from the 25%N-75%H group reached puberty earlier than heifers from other genetic groups supplemented with G1250 diet (P?<?0.05). Heifers with higher degree of Nelore (75%N-25%H) fed with G1000 diet showed estrus 42 days prior to mating, but only 57 % reached puberty at mating (P?<?0.05). Heifers with follicles of higher diameter reached puberty (P?<?0.05) earlier. Higher average daily weight gain showed a positive effect on follicular diameter and IGF-I level at puberty (P?<?0.01). Concentrations of GH were lower in heifers fed G1250 compared to G1000 diet (P?<?0.05). There was a significant interaction between nutritional level and insulin levels at puberty (P?<?0.01). We demonstrated the relationship between IGF-I and average daily gain on the onset of puberty in heifers. In conclusion, heifers submitted to the higher feeding level showed a higher follicular diameter and were younger at puberty. 相似文献
116.
Orla M Keane Jason D Keegan Barbara Good Theo de Waal June Fanning Michael Gottstein Mícheál Casey Christine Hurley Maresa Sheehan 《Irish veterinary journal》2014,67(1):16
Background
In 2013 a Technology Adoption Program for sheep farmers was established to encourage the implementation of best management practices on sheep farms in Ireland. There were 4,500 participants in this programme in 2013. As part of this programme, farmers had the option to carry out a drench test to establish the efficacy of their anthelmintic treatment.Results
Flock faecal samples were collected before and after treatment administration and gastrointestinal nematode eggs enumerated. In total there were 1,893 participants in the task, however only 1,585 included both a pre- and post-treatment faecal sample. Of those, 1,308 provided information on the anthelmintic product that they used with 46%, 23% and 28% using a benzimidazole (BZ), levamisole (LEV) and macrocyclic lactone (ML) product respectively. The remaining farmers used a product inapplicable for inclusion in the task such as a flukicide or BZ/LEV combination product. Samples were included for analysis of drench efficacy if the pre-treatment flock egg count was ≥200 eggs per gram and the interval post-sampling was 10–14 days for BZ products, 4–7 days for LEV products and 14–18 days for ML products. These criteria reduced the number of valid tests to 369, 19.5% of all tests conducted. If the reduction post-treatment was ≥95% the treatment was considered effective. Only 51% of treatments were considered effective using this criterion. There was a significant difference in efficacy between the anthelmintic drug classes with BZ effective in only 30% of treatments, LEV effective in 52% of cases and ML effective in 76% of cases.Conclusions
Gastrointestinal nematode anthelmintic treatments, as practiced on Irish farms, have a high failure rate. There was a significant difference between the efficacies of the anthelmintic classes with BZ the least effective and ML the most effective. 相似文献117.
Pedro Soler-Lloréns Yolanda Gil-Ramírez Ana Zabalza-Baranguá Maite Iriarte Raquel Conde-álvarez Amaia Zú?iga-Ripa Beatriz San Román Michel S Zygmunt Nieves Vizcaíno Axel Cloeckaert María-Jesús Grilló Ignacio Moriyón Ignacio López-Go?i 《Veterinary research》2014,45(1):72
Brucella spp. are Gram-negative bacteria that behave as facultative intracellular parasites of a variety of mammals. This genus includes smooth (S) and rough (R) species that carry S and R lipopolysaccharides (LPS), respectively. S-LPS is a virulence factor, and mutants affected in the S-LPS O-polysaccharide (R mutants), core oligosaccharide or both show attenuation. However, B. ovis is naturally R and is virulent in sheep. We studied the role of B. ovis LPS in virulence by mutating the orthologues of wadA, wadB and wadC, three genes known to encode LPS core glycosyltransferases in S brucellae. When mapped with antibodies to outer membrane proteins (Omps) and R-LPS, wadB and wadC mutants displayed defects in LPS structure and outer membrane topology but inactivation of wadA had little or no effect. Consistent with these observations, the wadB and wadC but not the wadA mutants were attenuated in mice. When tested as vaccines, the wadB and wadC mutants protected mice against B. ovis challenge. The results demonstrate that the LPS core is a structure essential for survival in vivo not only of S brucellae but also of a naturally R Brucella pathogenic species, and they confirm our previous hypothesis that the Brucella LPS core is a target for vaccine development. Since vaccine B. melitensis Rev 1 is S and thus interferes in serological testing for S brucellae, wadB mutant represents a candidate vaccine to be evaluated against B. ovis infection of sheep suitable for areas free of B. melitensis. 相似文献
118.
A. C. O. Dearo G. de Moraes Marcondes J. C. O. Araújo C. E. S. Grumadas P. C. Marino W. T. Teixeira G. de La Penha Chiacchio Fernandes 《Equine Veterinary Education》2014,26(12):640-644
Strangulating obstruction of the small intestine by anomalous mesenteric bands is an uncommon cause of colic in horses. The most commonly reported bands are mesodiverticular bands that are embryological remnants of the vitelline arteries. Despite the congenital nature of the anomaly, a wide age range of horses can be affected. This report describes a case of small intestinal strangulating obstruction caused by an unusual, anomalous congenital band attached to the ileocaecal fold in a yearling colt. Information including case background, history, clinical, laboratory, surgical and pathological findings is described. 相似文献
119.
Rotifers were fed on a laboratory-cultured alga, Platymonas (= Tetraselmis) suecica, and/or a diet composed of commercial single-cell proteins. They were given to sea-bass larvae up to day 15–20 after hatching, while Artemia nauplii were supplied from day 9–13. At day 21, the survival rate of fish obtained with rotifers fed on compound diet ranged between 32 and 82%, and their mean weight between 5.0 and 7.8 mg. When rotifers were fed either only on algae or on a mixture of 33% algae and 67% compound diet, the growth and survival rates of sea-bass were not clearly different to those obtained with rotifers fed on compound diet. No significant difference was observed when these rotifers were enriched with nutrients just before distributing them to fish. However, these rates appeared to be quite high in comparison to those obtained by several other authors. We can therefore recommend rotifers fed on compound diet as being, even without enrichment, convenient and low-cost food for sea-bass larvae. 相似文献
120.
The effects of temperature and salinity on the reproductive success of Arctic charr, Salvelinus alpinus (L.), were examined by holding broodstock under the following conditions from mid‐May until the end of September: fresh water at ambient temperature (NFW; 8–16 °C); salt water (25–30‰) at ambient temperature (NSW; 4–10 °C); fresh water cooled to saltwater temperature (CFW; 4–10 °C); or salt water heated to freshwater temperature (HSW; 8–16 °C). The relative fecundity of females was similar among groups (P > 0.05; 2685 ± 706 eggs), but females reared in NSW produced significantly larger eggs than those raised in NFW. The highest spermatozoa concentrations were found in milt from males reared in SW and the highest milt glucose concentration was from males kept under coldwater conditions (CFW, NSW). Eggs from NSW and HSW females contained more proteins than eggs produced by NFW females. Eggs from NSW females also contained 40% more lipids than was observed in the other groups, and total energy content was 27% higher in eggs from NSW females than in eggs from NFW females. When FW was cooled (CFW), females produced eggs with protein contents similar to those in NSW, but the lipid contents remained 30% lower. Finally, the best survival at the eyed stage and at hatch was observed in families produced by NSW broodstock. Intermediate values were observed in families from NFW or CFW while the highest mortality occurred in families from the HSW group. All these results suggest that, under the experimental conditions used in the present study, coastal seawater conditions offered the most favourable summer rearing conditions with respect to the reproductive success of Arctic charr. 相似文献