UV/H2O2降解水体中磷酸三（1,3-二氯-2-丙基）酯的研究

以磷酸三(1,3-二氯-2-丙基)酯(TDCP)作为研究对象,探索了TDCP的紫外光氧化高效降解方法。结果表明,紫外光光强对TDCP的降解有很大的影响。当TDCP初始浓度为10 mg/kg,紫外光光强为94.5×100μW/cm2,反应时间为60 min,加入H2O2浓度为5.00 mmol/L,溶液起始pH为7时,TDCP的TOC去除率为93.69%,有机氯、有机磷完全转化为Cl-和PO43-;UV/H2O2体系能有效的降解TDCP。     

Cloning and Sequence Analysis of Buffalo Keap1 Gene and Investigation of Its Expression Pattern in Different Tissues

In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture.     

动物医学临床教学数据库的构建与初步应用

动物医学专业不仅要求学生掌握扎实的理论知识,还需要具有丰富的临床实践经验。为了实现该目标,除了要求授课教师对专业知识融会贯通,更需要拥有大量典型的临床病例资料。以安徽农业大学动物医院和畜禽疾病诊断中心为平台,收集大量宝贵的临床病例资料和最新的临床诊疗技术,构建了动物医学临床教学数据库,使得诊疗动物种类多样化,案例信息全面化、系统化。该数据库的建成和应用,为临床教师提供了丰富的教学素材和资料,亦提升了学生的学习兴趣,促进了动物医学专业的教育教学发展。     

半夏种茎规格对产量、品质及植株的影响

在喀斯特温和气候区,采取小区试验方法,按半夏[Pinellia ternate(Thunb.)Breit.]种茎直径≥2.0cm、1.5 cm≤种茎直径<2.0 cm、1.0 cm≤种茎直径<1.5 cm、种茎直径<1.0 cm 4种规格种植,以块茎鲜重产量、总生物碱含量与种茎规格大、中、小的关系为考察依据,研究半夏不同规格种茎种植对块茎产量和总生物碱含量的影响。结果显示,随着种茎规格的增大,半夏块茎鲜重产量呈直线上升,以种茎直径≥2.0cm的产量最高,为2.44 kg/m2。净产量以1.5≤种茎直径<2.0 cm的最高,为0.98 kg/m2;总生物碱含量以1.0 cm≤种茎直径<1.5 cm的最高,为0.302 mg/g,二者均呈开口向下的抛物线变化。考虑到兼顾块茎产量和总生物碱含量,半夏种植以种茎直径为1.0～1.5 cm的较好。     

新农村建设中农村体育发展的困境与对策

以建设社会主义新农村为背景,用文献资料法、观察法、访谈法等多种方法,全面分析了体育运动在新农村建设中所能起到的作用,总结了目前农村体育发展所面临的困境,阐明了在新农村建设中农村体育发展能够获得的机遇,提出了发展新农村体育运动可以运用的策略。     

十种含浙贝母中成药中的二氧化硫残留量分析

采用《中国药典》2015年版四部通则2331二氧化硫残留量测定法第一法对10种含浙贝母(Fritillaria thunbergii)中成药中的二氧化硫残留量进行测定和分析。结果表明,有2个厂家的2种中成药的二氧化硫超标,合格率为86.7%。     

Cloning and Sequence Analysis of HA and NA Genes of One Strain of H6N6 Subtype Avian Influenza Virus Isolated from Guizhou Province

To investigate the epidemic situation of H6N6 subtype avian influenza virus (AIV) in Guizhou province,A/duck/Guizhou/013/2014 was isolated from Sansui duck in live poultry market of Guizhou in 2014,the hemagglutinin (HA) and neuraminidase (NA) genes of DK/GZ/14 were subjected to clone and sequence analysis.The results showed that HA gene had the highest nucleotide homologies (97.5%) with the duck-origin H6N6 subtype AIV isolated from Eastern China in 2009,and the strains of HA gene proteolytic cleavage sites was P-Q-I-E-T-R-G,which accordeol with the molecular characteristic of low pathogenic AIV (LPAIV).However,NA gene of A/duck/Guizhou/013/2014 had the highest nucleotide homologies (98.2%) with the duck-origin H6N6 subtype AIV isolated from Fujian in 2007.The phylogenetic tree showed that A/duck/Guizhou/013/2014 and Hunan strains located in the same branch,while three duck-origin H6N6 subtype AIV isolated from Guizhou in 2007 and A/duck/Guizhou/013/2014 located in the different branch for HA and NA genes in genetic evolution,which suggested that A/duck/Guizhou/013/2014 was far with the local H6N6 subtype.The results also clearly indicated that duck-origin H6N6 subtype AIV had genetic diversity in duck population in Guizhou.     

22~42日龄黄羽肉鸡饲粮苏氨酸需要量研究

【目的】通过研究饲粮苏氨酸水平对22~42日龄黄羽肉鸡生长性能、胴体品质和免疫功能的影响,确定其苏氨酸需要量。【方法】试验选用22日龄快大型岭南黄羽肉鸡1 600只,根据体重相同的原则分为5个组,每组8个重复,每个重复40只肉鸡（公母各半）。采用玉米-花生粕型基础饲粮,5个处理组试验鸡分别饲喂含0.60%（基础饲粮）、0.67%、0.74%、0.81%和0.88%苏氨酸饲粮,试验期为21 d。试验结束后,每个重复选取2只鸡屠宰采样,测定屠宰性能、免疫器官指数和血清生化指标。【结果】饲粮苏氨酸水平显著影响黄羽肉公鸡、母鸡的体重、平均日增重和料重比（P<0.05）。对于母鸡,0.88%苏氨酸水平组平均日增重最高;0.81%苏氨酸水平组平均日采食量最高。对于公鸡,0.81%苏氨酸水平组平均日增重最高;0.81%苏氨酸水平组平均日采食量最高。饲粮苏氨酸水平与黄羽肉公鸡平均日增重、平均日采食量以及公、母鸡料重比呈显著的二次线性相关（P<0.05）;根据平均日增重和平均日采食量回归方程得出黄羽肉公鸡饲粮苏氨酸最适水平分别为0.81%和0.83%;根据料重比回归方程得出黄羽肉母鸡和公鸡饲粮苏氨酸最适水平分别为0.83%和0.86%。饲粮苏氨酸水平对黄羽肉公鸡、母鸡的全净膛率、半净膛率、腿肌率、胸肌率均无显著影响（P>0.05）;对黄羽肉公鸡全净膛率和腹脂率均有显著影响,其中0.81%苏氨酸组全净膛率显著高于0.60%和0.74%苏氨酸组,0.88%苏氨酸组腹脂率显著低于0.60%和0.67%苏氨酸组（P<0.05）。饲粮苏氨酸水平显著影响黄羽肉母鸡法氏囊指数和胸腺指数,其中0.60%苏氨酸组法氏囊指数显著低于0.81%苏氨酸组,0.81%和0.88%苏氨酸组胸腺指数显著高于0.60%苏氨酸组（P<0.05）;对肝脏指数、脾脏指数无显著影响（P>0.05）。饲粮苏氨酸水平对黄羽肉母鸡血清中甘油三酯、胰岛素含量有显著影响,其中0.60%苏氨酸水平组血清中胰岛素含量最低,0.88%苏氨酸水平组血清中甘油三酯含量最低,显著低于0.60%苏氨酸组（P<0.05）;苏氨酸水平对黄羽肉公鸡甘油三酯含量有显著影响,其中0.88%苏氨酸水平组血清中甘油三酯含量最低,显著低于0.60%和0.67%苏氨酸组（P<0.05）。【结论】饲粮中添加适量苏氨酸可以提高黄羽肉鸡生长性能、胴体品质,促进免疫器官发育。以生长性能为主要判定指标,确定22~42日龄黄羽肉母鸡饲粮最适苏氨酸水平为0.83%,黄羽肉公鸡最适苏氨酸水平为0.81%。     

Analysis on the Claims Caused by the Defect of Contract

The engineering contract is composed of many documents between client and contractor, such as agreement, notification of award, tender and appendix to bid, conditions for particular application, instruction to bidders, technical specifications, design drawings and bill of quantities etc. Because of the limited time and money to prepare these documents, the defect is unavoidable. The defects include omittance, equivocalness, conflict and mistake. These defects often lead to claims.     

柴油机机体有限元结构分析

以柴油机机体为研究对象,利用ANSYS软件构建了精确的机体有限元接触模型,根据试算和经验确定了位移和载荷边界条件,对机体在各缸最大爆发压力下进行了结构强度和刚度分析,得到了机体在最大爆发压力下的最大变形点和最大应力点,分析了机体、气缸套、挺柱孔、轴瓦各处的变形和应力分布,考查了机体顶面和底面的密封性,并对原机体结构提出了改进建议。