Detection of calves persistently infected with bovine pestivirus in a sample of dairy calves in south-eastern Queensland

Objective To determine the proportion and incidence of calves persistently infected with bovine pestivirus in calves (n = 1521) supplied to the Tick Fever Research Centre and to assess the test regime to detect calves persistently infected with bovine pestivirus.
Design Calves, 1 to 6 weeks old, selected for use in the production of the tick fever vaccine were collected from 21 properties in 56 separate groups between October 1990 and December 1996. Each group was examined for the presence of calves persistently infected with bovine pestivirus.
Procedure All calves were routinely tested for antibody to bovine pestivirus and bovine pestivirus antigen using a serum neutralisation test and an antigen-capture ELISA, respectively. Pooled lymphocyte samples from calves were also monitored for bovine pestivirus by inoculation of sheep. Whole herd testing was carried out in eight herds, using a serum neutralisation test as a screen test followed by an antigen-capture ELISA of cattle with a serum neutralisation test titre of less than 32.
Results Fourteen of the 1521 calves tested (0.9%), were detected as persistently infected and the incidence ranged from 0.0 to 3.0 % per year over 6 years. Persistently infected calves were found in 13 of the 59 groups and originated from 7 of the 21 herds used. In whole herd testing on the properties of origin, cattle persistently infected with bovine pestivirus were detected in four of the eight herds tested
Conclusions The proportion of calves persistently infected with bovine pestivirus is similar to that in other countries and indicates that bovine pestivirus could be a significant cause of economic loss in Australian cattle herds. In detecting calves persistently infected with bovine pestivirus, the combination of sheep inoculation, paired antigen-capture ELISA and serum neutralisation tests appeared to be highly sensitive and specific.     

Equine thyroid dysfunction.

Hypothyroidism is the most common type of thyroid gland dysfunction reported in horses. Primary, secondary, and tertiary causes of hypothyroidism are discussed. Equine hypothyroidism remains a controversial endocrine disorder because extrathyroidal factors, including the administration of drugs and systemic diseases, affect serum triiodothyronine (T3) and thyroxine (T3) concentrations in horses. Accurate diagnosis of hypothyroidism therefore requires assessment of the hypothalamic-pituitary-thyroid axis. Diagnostic procedures for evaluating thyroid gland function are outlined and results of studies utilizing experimental models are discussed.     

Conservation tillage for soybean in the U.S. Southeastern Coastal Plain

Double cropping of soybean has progressed less rapidly in the U.S. Southeastern Coastal Plains than expected by the ample rainfall and long frost-free season. Post-emergence herbicides, the management of plant residues to reduce water use by cover crops, and a no-till planter with a combination subsoiler are the innovations that have facilitated this new production. Full-season soybean (Glycine max L.) was planted following a grazed cover crop of winter rye (Secale cereale L.) or late-season soybean was planted following winter wheat harvest. In both cases, a special planter was used with an integral subsoil shank ahead of the opener. Full-season soybean under conservation tillage produced yields equal to or better than yields in conventional clean tillage. In a dry summer, soybean yields under conservation tillage exceeded conventional tillage because of suppressed early biomass production which conserved stored soil water and favored growth during the reproduction phase of the crop-cycle. Late-season soybean yields behind wheat favored the conservation tillage practice of in-row subsoil-planting into stubble. However, planting in burned-off wheat stubble produced the highest yields in this study. In a dry spring, the cover crop accelerated soil water use which resulted in lower soybean yields under conservation tillage. Comparisons of 76 vs. 97 cm row spacing were inconclusive, but the trend suggests that wider rows conserve water under periods of drought and that the narrower-row configuration favors adequate water regimes.     

Bragg soybeans grown on a southern coastal plain soil IV. Seasonal changes in nodal N and P concentrations

Determinate soybean [Glycine max (L.) Merr.] has been characterized by few detailed nitrogen and phosphorous partitioning studies. Knowledge of the variation in N and P concentrations with plant part, nodal position, and plant age is needed for a better understanding of plant functions. In this field study, ‘Bragg’ soybean was grown on an Aquic Paleudult soil (series Goldsboro loamy sand). Plants were sampled at 10 to 14 day intervals beginning 44 days after planting (July 7) until harvest. Maximum observed N concentrations were 3.1, 2.8, 5.8, and 5.4% for stem internodes, petioles (+branches), leaf blades, and pods, respectively. Maximum observed P concentrations were 0.34, 0.48, 0.78, and 0.52 for the same respective plant parts. Nodal and temporal mean N and P concentrations varied considerably with plant age and nodal position in all plant parts. These data show that mean N and P concentrations in all four plant parts can vary several fold, depending upon plant age and nodal position for the sample. This suggests caution should be exercised in tissue sampling and interpretation of plant analysis. Concentrations of N and P generally decreased with time for stem internode, petioles (+branches), and leaf blades, but increased with time for pods. Except for N concentration in stem internodes, which increases with internode number, the N and P concentrations remain nearly constant throughout the growing season. The relationships provide insight for developing accurate plant models depicting N and P concentrations and translocations over time and among plant parts in determinate soybean.     

Serotypes of Erysipelothrix rhusiopathiae in Australian pigs, small ruminants, poultry, and captive wild birds and animals

Serotypes of 93 Australian isolates of Erysipelothrix rhusiopathiae from diseased domestic animals and poultry and a variety of captive wild birds and animals were determined by double diffusion gel precipitation. Two isolates, from the faeces of a swallow were also examined. Serotypes 1a, 1b and 2 were isolated from pigs and serotypes 1a, 1b, 2, 5, 15 and 21 from sheep or goats. Erysipelas in poultry was attributed to serotypes 1b, 5, 15 and 16. In captive wild birds serotypes 1b, 5, 6, 8, 14, 21 and an isolate reactive with antiserum to strain Seehecht were associated with septicaemic deaths. Single isolates from tissues of a bilby (Macrotis lagotis), black rat (Rattus rattus), brown snake (Pseudechis australis) and a bandicoot (Isoodon macrouris) were classified as serotypes 4, 4, 7, and 10 respectively. Six isolates were not able to be typed. Serotype 1b was the most widely distributed and most common (28%), being associated with disease in pigs, sheep, poultry and wild birds. Serotypes 1a or 2 were found in a more restricted range of animals, being commonly associated with erysipelas in pigs, less commonly in sheep and infrequently in other species. From diseased pigs, 26 of 33 isolates (79%) were serotypes 1a and 1b.