Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region,China

Ammonia oxidizing （AOB） and denitrifying bacteria （DNB） play an important role in soil nitrogen transformation in natural and agricultural ecosystems. Effects of long-term fertilization on abundance and community composition of AOB and DNB were studied with targeting ammonia monooxygenase （amoA） and nitrite reductase （nirK） genes using polymerase chain reaction- denaturing gradient gel electrophoresis （PCR-DGGE） and real-time PCR, respectively. A field trial with different fertilization treatments in a rice paddy from Tai Lake region, centre East China was used in this study, including no fertilizer application （NF）, balanced chemical fertilizers （CF）, combined organic/inorganic fertilizer of balanced chemical fertilizers plus pig manure （CFM）, and plus rice straw return （CFS）. The abundances and riehnesses of amoA and nirK were increased in CF, CFM and CFS compared to NF. Principle component analysis of DGGE profiles showed significant difference in nirK and amoA genes composition between organic amended （CFS and CFM） and the non-organic amended （CF and NF） plots. Number of amoA copies was significantly positively correlated with normalized soil nutrient richness （NSNR） of soil organic carbon （SOC） and total nitrogen （T-N）, and that of nirK copies was with NSNR of SOC, T-N plus total phosphorus. Moreover, nitrification potential showed a positive correlation with SOC content, while a significantly lower denitrification potential was found under CFM compared to under CFS. Therefore, SOC accumulation accompanied with soil nutrient richness under long-term balanced and organic/inorganic combined fertilization promoted abundance and diversity of AOB and DNB in the rice paddy.     

Optimization of irrigation and nutrient concentration based on economic returns,substrate salt accumulation and water use efficiency for tomato in greenhouse

To evaluate the effects of different irrigation and nutrient concentration strategy on growth, yield, water use efficiency (WUE), fruit quality and substrate salt accumulation, tomatoes were grown with five different levels of water (W: 50%, 75%, 100%, 125% and 150%) and nutrient concentrations (N: 0.5, 0.75, 1.0, 1.5 and 2.0 times of Hoagland strength(X)). Fruit quality index was determined by normalization of fruit quality parameters. Deficit irrigation at standard concentration of nutrients reduced yields by 17.43% and 15.52% for T₇ (W_75%-N_1.0x) and 49.54%–51.99% for T₈ (W_50%-N_1.0x) during spring-summer (SS) and fall-winter (FW) seasons, respectively. Contents of total soluble solids (TSS), titrable acidity (TA) and sugar acid ratio (SAR) were all increased in water-deficit treatments. T₈ was found to be highest in TSS, TA and SAR except SAR in FW. Over-irrigation with excessive and standard Hoagland nutrient concentration caused non-significant reduction in yield except T₆ (W_125%-N_1.0x) in SS. T₂ (W_100%-N_2.0x) and T₄ (W_100%-N_1.5x) caused more substrate salt accumulation which resulted in significant decrease in yield and WUE. Through economic analysis, over-watering along with excessive nutrients caused profit reductions. Considering water saving, yield and fruit quality through economic analysis, T₇ found to be optimal strategy.     

Effects of dietary energy level on growth performance,blood parameters and meat quality in fattening male Hu lambs

This experiment was conducted to investigate the effects of different dietary energy levels on growth performance, slaughter traits, meat quality and blood biochemical parameters in fattening male Hu lambs. Sixty lambs were fed five iso-protein diets which contained different levels of metabolizable energy in a completely randomized design for 70 days. At the end of study, fifteen lambs were harvested for further study. With the increase in dietary energy level, the daily weight gain and dry matter intake extremely increased (p < .001), and feed conversion ratio decreased significantly (p < .01). The live weight before slaughter (LWBS) and carcass weight had a significant increase (p < .05), non-carcass fat ratio and routine indexes of meat quality had no significant difference. Almost no effect was observed for the amino acid profile except for glycine concentration decreased (p < .05) in longissimus dorsi (LD) muscle. The concentrations of C17:0, C18:3n-3 and n-3 PUFA (p < .01) significantly decreased with the increasing dietary energy levels, and the ratio of ∑n-6/∑n-3 (p < .01) increased, whereas the concentrations of C18:1n-9t (p < .05) decreased in LD muscle and C18:1 (p < .01) increased in biceps femoris (BF) muscle. There were no obviously differences for diameter, area and density of muscle fibres. The relative expression of MyHC-IIa and MyHC-IIx decreased significantly (p < .05) in BF muscle, and the relative expression of MyHC-IIa displayed an obviously decreasing trend (p < .10) in LD muscle. These results suggest that increasing the dietary energy level can improve the growth performance and slaughter traits, and influence meat quality and fatty acid profiles in different muscle tissues of fattening male Hu lambs. These results provide a theoretical basis for developing Hu sheep nutritional standards and designing feed formulations.     

Depth Distribution of Soil Organic Carbon Fractions in Relation to Tillage and Cropping Sequences in Some Dry Lands of Punjab,Pakistan

Depth distribution of soil organic carbon (SOC) fractions depends on the efficiency of agro‐technical managements. Information on depth distribution of SOC fractions mostly confined to the plow layer and scant in dry lands of Punjab, Pakistan. Therefore, a field experiment was laid out with moldboard plow (MP) (control), tine cultivator (TC), and minimum tillage (MT) as main plots, and cropping sequences fallow wheat (Triticum aestivum L.), (FW, control), mungbean (Vigna radiata L.) wheat (MW), sorghum (Sorghum bicolor L.) wheat (SW), green manure wheat (GW), and mungbean‐chickpea (MC) (Cicer arietinum L.) as sub‐plots. Treatment effects were assessed for microbial biomass carbon (MBC), potentially mineralizeable carbon (PMC), particulate organic carbon (POC), dissolved organic carbon (DOC), HCl insoluble carbon (HIC), and stratification ratio (SR) in Rawal series: Udic Haplustalf. Alfisols. The MBC concentration was the highest in MT system, at 15 to 30‐cm depth under MW and PMC concentration was highest under SW with MT at 45–60 cm. MP had higher POC in FW sequence. The highest DOC was at 0 to 15‐cm depth under MC with TC and stock of HIC was more under TC with FW sequence. The highest SR of PMC was under MT with FW at 0–15:15–30 and POC was under TC and MP with FW at depths of 0–15:45–60 cm. The highest SR for DOC was under MP with GW at 0–15:45–60 cm and HCl insoluble C was under MT with SW at 0–15:45–60. In broad‐spectrum, labile organic fractions revealed differential sensitivity, and POC stocks are also a sensitive indicator to detect the short‐management effects. Copyright © 2015 John Wiley & Sons, Ltd.     

Efficiency of KOH-modified rice straw-derived biochar for reducing cadmium mobility, bioaccessibility and bioavailability risk index in red soil

Cadmium (Cd) pollution in agricultural soils has exerted a serious threat due to continuous application of pesticides, fertilizers, and wastewater irrigation. The present study aimed to test the efficiency of KOH-modified and non-modified rice straw-derived biochar (KBC and BC, respectively) for reducing Cd solubility and bioavailability in Cd-contaminated soil. Cadmium-contaminated soil was incubated for 60 d with 15 and 30 g kg^-1 BC and KBC. At the end of incubation, Cd mobility was estimated by the European Community Bureau of Reference sequential extraction and toxicity characteristic leaching procedure (TCLP), while bioavailability was determined using 1 mol L^-1 NH₄NO₃ extraction. The bioavailability risk index and bioaccessibility, assessed by a simple bioaccessibility extraction test, of Cd were used to examine the potential effects of Cd on living organisms. The results indicated that application of both KBC and BC significantly increased soil pH, cation exchange capacity, nutrients, and organic carbon. The soluble fraction of Cd was significantly decreased by 30.3% and 27.4%, respectively, with the addition of KBC and BC at 30 g kg^-1 compared to the control (without biochar addition). Similarly, the bioaccessible Cd was significantly decreased by 32.4% and 25.2%, respectively, with the addition of KBC and BC at 30 g kg^-1 compared to the control. In addition, both KBC and BC significantly reduced Cd leaching in the TCLP and NH₄NO₃-extractable Cd in the amended soil compared to the control. The reduction in Cd solubility and bioaccessibility by KBC and BC may be due to significant increases in soil pH and surface complexation. Overall, KBC at an application rate of 30 g kg^-1 demonstrated positive results as soil amendment for Cd immobilization, and reduced bioaccessible Cd in contaminated soil.     

Relationship between gut microbiota and host-metabolism: Emphasis on hormones related to reproductive function

It has been well recognized that interactions between the gut microbiota and host-metabolism have a proven effect on health. The gut lumen is known for harboring different bacterial communities. Microbial by-products and structural components, which are derived through the gut microbiota, generate a signaling response to maintain homeostasis. Gut microbiota is not only involved in metabolic disorders, but also participates in the regulation of reproductive hormonal function. Bacterial phyla, which are localized in the gut, allow for the metabolization of steroid hormones through the stimulation of different enzymes. Reproductive hormones such as progesterone, estrogen and testosterone play a pivotal role in the successful completion of reproductive events. Disruption in this mechanism may lead to reproductive disorders. Environmental bacteria can affect the metabolism, and degrade steroid hormones and their relevant compounds. This behavior of the bacteria can safely be implemented to eliminate steroidal compounds from a polluted environment. In this review, we summarize the metabolism of steroid hormones on the regulation of gut microbiota and vice-versa, and also examined the significant influence this process has on various events of reproductive function. Altogether, the evidence suggests that steroid hormones and gut microbiota exert a central role in the modification of host bacterial action and impact the reproductive efficiency of animals and humans.