基于植物能量功能群的草原植物光合作用研究

植物能量功能群是依植物能量属性进行的植物功能类群划分,植物通过光合作用将光能转变成化学能,蓄积在植物体内,为了探究不同的能量功能群是否会表现出光合作用特征的差异,用LI-6400型便携式全自动光合测定仪,在内蒙古锡林郭勒典型草原区,于7月底至8月初的植物生长旺盛期,测量了羊草(Leymus chinensis)、糙隐子...     

匍匐翦股颖HSP26.7基因启动子的表达分析

为研究匍匐翦股颖HSP26.7基因启动子在植物抗逆方面的功能,克隆了匍匐翦股颖的一个小热激蛋白HSP26.7基因的启动子并进行了序列分析;构建了同时具有该启动子序列和GUS报告基因的植物表达载体,并通过农杆菌侵染法转化拟南芥植株.结果显示:该启动子存在HSE,ABRE等响应高温和其他胁迫的调控元件,受高温胁迫强烈诱导,...     

祁连山黑河上游不同退化草地有机碳和酶活性分布特征

为揭示草地退化过程中土壤有机碳和酶活性的分布特征及相关性,在祁连山黑河上游俄博岭区域的高山灌丛草甸土上,根据草地退化程度,划分了未退化草地、轻度退化草地、中度退化草地、重度退化草地,采用野外采样室内分析方法,研究了不同退化草地土壤有机碳和酶活性的分布特征。结果表明：4种退化草地0～60 cm土层有机碳的含量、密度、储量,以及土壤酶活性变化顺序为：未退化草地>轻度退化草地>中度退化草地>重度退化草地;4种退化草地（从轻到重）0～10 cm土层有机碳密度是50～60 cm土层有机碳密度的1.78倍、1.98倍、1.87倍和2.00倍,说明土壤有机碳密度在表层具有很强的表聚性;4种退化草地不同土层有机碳的含量、密度和储量,以及土壤酶活性均随着土壤剖面垂直深度的增加而递减;4种退化草地土壤有机碳含量与有机碳密度、土壤酶活性成显著正相关关系。     

1株奶牛源松鼠葡萄球菌的分离鉴定及耐药性分析

为确定奶牛垫料中是否含有病原菌并深入了解垫料中优势生长菌的类型、耐药性及致病性等情况,本试验进行了细菌分离培养、革兰染色镜检、生化试验鉴定、16S rDNA序列分析及同源性比对、药敏试验、小鼠致病性试验。结果显示,分离菌在普通营养琼脂平板上形成圆形、表面光滑的白色菌落,血琼脂平板上形成黏稠、较大的白色菌落。革兰染色镜检结果显示,分离菌为革兰阳性,球型,呈葡萄串状排列,或单个散落。生化试验结果显示,分离菌对木糖、葡萄糖、麦芽糖、果糖、乳糖、硝酸盐还原等呈阳性反应,而蜜二糖、木糖醇、山梨醇、尿素、V-P反应等呈阴性反应。16S rDNA序列分析结果显示,扩增的16S rDNA序列长度为1 298 bp,与松鼠葡萄球菌的核苷酸同源性达99.85%~100%;系统进化树结果显示,分离菌与松鼠葡萄球菌处于同一分支。动物试验结果表明,分离菌株对试验小鼠有较强的致病性,以0.2 mL/只（7.9×10⁸ CFU/mL）菌液的剂量接种小鼠,在48 h内死亡率为60%（3/5）。药敏特性分析结果显示,分离菌株对复方新诺明、氨苄西林等7种药物敏感,对克林霉素、头孢噻肟和头孢呋辛中度敏感,对青霉素、红霉素和林可霉素耐药。本研究为奶牛源松鼠葡萄球菌的分离鉴定及防控提供了参考依据。     

拷贝数变异在畜禽中的研究进展

拷贝数变异（copy number variation,CNV）具有多种形式的变异结构,在品种多样性、生物进化和疾病相关性等研究中起着重要作用,并具有片段长度大、覆盖范围广等特点。随着分子生物学的发展及DNA测序技术的日渐成熟,人们对遗传变异的研究不断向DNA分子水平深入,多态性标记在畜禽育种中已逐渐成为动物育种研究的趋势和主流。由于CNV对基因的调控和表达所造成的影响更为显著,因此,CNV在重要畜禽中的研究越来越多。目前,已检测出大量有关畜禽重要经济性状的基因序列变异,并有许多研究均表明CNV与动物的重要经济特征及疾病的发生有关。笔者主要通过参考国内外相关的研究报道,简述了CNV的相关研究背景、概念、突变机制,归纳总结了CNV对牛、羊、猪、鸡的经济性状、繁殖性状和疾病调控的影响,以期通过对这些重要畜禽的基因组学研究揭示其适应性遗传机理和表型性状差异的遗传基础,开发相应的分子遗传标记,为畜禽的标记辅助选育提供理论基础。     

Control Strategy and Simulation Study on NiMH Battery Quick Charging for Regenerative Braking of HEV

Based on the result of NiMH battery performance experiments, changing trend of maximal temperature and difference in temperature of HEV NiMH battery in different SOC and different charging current is analyzed. Considering the working situation of HEV NiMH battery and the theory of battery quick charging, a control strategy for NiMH battery quick charging is described which adapts to HEV regenerative braking system based on Mascc law. By Modeling and simulating the process of NiMH battery quick charging with this strategy, constant voltage charge method and constant current charge method, validates this control strategy.     

Design of Data Acquisition System for Vehicle Based on CAN Bus

To the question that a meter is correspond with a set of lead and a sensor,this paper presents a design of data acquisition system for vehicle based on CAN bus.In this way,the combinatorial instrument is only connected by a bus unit.Author introduces the data acquisition structure chart and the CAN interface,analyzes the software by program flow chart,and shows it's application for vehicle virtual instrument.A method of calculating vehicle speed,engine rotate speed and the course of development mileage are also given.     

Dynamics of Actin Polymerization

The process of actin polymerization and depolymerization have some relation with its function,the activation of such process is compatible to its functions. The polymerization progress is divided into four steps: The first step is the activation of the monomer actin. The nucleation of actin nucleus forms in the second step which limits the polymerization speed. The elongation of the F-actin filaments happens in the following step. In the last step, a steady-state is attained, the length of the filament does not change because the polymerizing rate in the barbed end is the same with the depolymerizing rate in the pointed end. Some factors which affect the polymerizing process, such as nucleotide, actin related proteins are discussed. The aim is to know more about how to assemble the complicated system with biomacromolecule and what to function.     

Portable ECG Monitor Front- ended Design with C8051 F020

The electrocardiogram (ECG) signal is a recording of the electrical activity on the body surface generated by the heart frequency of which is weak and low. Many researchers devote themselves to get more perfect ECG in many ways. This article introduces the characterize of an ECG signal , then discusses the requirements for the sampling circuit of an ECG. Since there are some shortcoming in common design means, the authors give an effective circuit. The system is composed of a low-cost , low-power and high-performance MCU C8051F020 , amplifier AD620 , right-leg drive circuit ,communication circuit and power management circuit. Then software design is given which is used to sampling and filtering ECG signal. Levkov filter arithmetic and smooth filter arithmetics are used in this design. In the end , the authors advance a distributing and networking ECG monitor system.