发酵脱氢产氢过程对微生物铁还原的影响

发酵型微生物是铁还原菌中的主要类群,但其发酵产氢过程对铁还原的作用尚不清楚,为此采用接种水稻土浸提液混合培养的方法对微生物分别利用葡萄糖、丙酮酸盐和乳酸盐为碳源时,Fe（Ⅲ）还原过程中脱氢酶活性变化、培养体系pH、氢气分压及铁还原特征进行分析,探讨了发酵微生物脱氢产氢过程与微生物Fe（Ⅲ）还原的内在关系。结果表明：2种水稻土浸提液中的微生物均能够以葡萄糖为优势碳源进行脱氢、产氢及还原氧化铁,Fe（OH）,可以诱导脱氢酶的产生,利用葡萄糖时脱氢酶活性在厌氧培养的4-6d出现最大峰值,利用丙酮酸盐和乳酸盐时脱氢酶活性出现峰值的时间分别为培养的15d和21-22d,脱氢酶活性出现峰值的时间与最大铁还原速率Vmax显著负相关、与最大反应速率对应的时间zk存在显著正相关关系。脱氢产氢过程中产生的H＋导致培养体系pH的变化是影响铁还原过程的主要原因,培养体系pH与体系氢气分压及Fe（Ⅱ）累积量呈极显著负相关。微生物利用不同碳源产氢时,利用葡萄糖的产氢能力最高,丙酮酸盐次之,乳酸盐最低。Fe（OH）3的加入增加了氢气的消耗量,培养体系氢气分压与Fe（Ⅱ）累积量存在极显著正相关关系。     

蓝藻生物节律性分子调控机制的研究进展

生物钟现象是一种普遍存在于生物界细胞的内源节律性保持机制。生物钟机制的存在可以使生物体的代谢行为产生并维持以24h为周期的昼夜节律,从而更好地适应于地球自转所产生的环境条件昼夜间节律性变化。蓝藻是目前生物钟分子机制研究中的模式生物,其依赖于kai基因家族成员的核心生物钟调控模式已经被众多研究者详细阐明。蓝藻生物钟的核心振荡器是由蓝藻kaiA／B／C的编码产物来调控的,Kai蛋白的表达模式具有节律性。KaiC蛋白磷酸化状态的节律性循环及输入、输出途径相关组成蛋白的翻译后修饰状态节律性循环共同组成其反馈回路,负责维持生物钟节律性振荡的持续进行并与环境周期保持同步。传统的蓝藻生物钟分子机制模型认为,节律性表达基因翻译产物的转录／翻译负反馈抑制环是生物节律性维持和输出的关键。遗憾的是,在其它物种生物钟分子机制研究中未发现由ka／基因家族成员同源基因组成的节律性标签,这表明以kaiA／B／C为核心振荡器的生物钟系统并不是一种跨物种保守的生物钟系统。近期,人们发现非转录／翻译依赖的振荡器（NTO）也具有成为生物节律性产生和维持的“源动力”的可能。过氧化物氧化还原酶（Pax）氧化还原状态节律性是第一种被报道的跨物种保守的NTO节律性标签,这也日渐成为蓝藻生物钟分子机制研究新的热点。     

基于无线传感网络的耕种机具空间力监测系统开发与试验

针对实时监测耕种机具作业中所受空间力大小,该文提出了一种能够在现场和远程实时监测耕种机具受力情况并带有提示报警的监测系统。该系统采用基于Zigbee的WSN（wireless sensor network）完成数据的采集和近程传输,采用嵌入式处理器ARM11作为现场监测终端的中央处理单元CPU,并搭建WinCE6.0操作系统,完成数据交换、处理和监测报警等功能。通过安装在耕种机具上的测力传感器,实现对耕种机具受力情况的实时检测。经土槽试验测试,系统实现了耕种机具空间力的实时监测以及监测数据的存储,人机界面交互性良好,可为耕种机具的研究设计提供技术支持。     

激光平地改善土壤水盐分布并提高春小麦产量

为了促进激光平地技术的应用与推广,在河套灌区以普通畦田为对照,分期、分段测定了激光平地畦田0～80 cm土层土壤水分、土壤盐分及春小麦生长状况。结果表明,从渠口到畦尾,激光平地畦田不同地段的土壤水分在春小麦生长的不同时期无显著差异,但从拔节期开始,上层土壤盐分逐渐升高;普通畦田从三叶期开始,上层土壤水分、盐分逐渐提高,且显著或极显著高于激光平地。普通畦田从三叶期开始,激光平地从拔节期开始,从渠口到畦尾,春小麦的生长状况逐渐变差,千粒质量、产量均逐渐降低,但激光平地的春小麦千粒质量、产量高于普通畦田。激光平地改善了畦田的土壤水分、盐分分布,促进了畦田中后段的春小麦生长,提高了畦田中后段的水分利用率及产出率。     

石灰岩山地不同整地组合对侧柏林蓄水保土功能的影响

以新泰市汶南镇洞山小流域石灰岩山地山坡上部采取6种整地组合营造的六年生侧柏林为研究对象,系统研究不同整地组合侧柏林的蓄水保土功能。结果表明:(1)6种整地组合侧柏林的林木保存率、林木生长量和枯落物层蓄积量以整地深度和面积最大的侧柏林的林木保存率和林木生长量最大,枯落物层蓄积量最多;林下灌草植被生物量随着林分郁闭度的减小而增加。枯落物分解后改善了土壤物理性状,增加了土壤孔隙度,减小了土壤容重,提高了土壤渗透速率,增加了土壤蓄水量。(2)6种整地组合侧柏林与对照相比减少的土壤侵蚀量以水平阶整地2最多,为39.161t/hm2,穴状整地1的较小,为34.153t/hm2。(3)对不同整地组合侧柏林的林分郁闭度、枯落物蓄积量、土壤总孔隙度、非毛管孔隙度、土壤渗透速率、增加的土壤饱和贮水量、减少的土壤侵蚀量、灌草生物量、灌草盖度和有机质含量等进行方差分析,可知均存在显著差异,按各项指标得分相加得出蓄水保土效益,不同整地组合侧柏林的蓄水保土功能排序为水平阶整地2>鱼鳞坑整地2>水平阶整地1>鱼鳞坑整地1>穴状整地2>穴状整地1。     

基于改进湍流模型和偏振成像技术的水下退化图像复原方法

为了满足工厂化水产养殖中过程控制的信息化要求,该文提出一种基于改进湍流模型结合偏振成像技术,具有较强鲁棒性的水下退化图像复原方法。考虑内外尺度对波结构函数影响,结合折射率谱,改进水下湍流退化模型以提高复原算法先验知识的完备性;基于改进的退化模型和水下前向散射光的偏振特性,利用偏振成像技术提取退化图像中的噪声特征;基于退化图像噪声特征,采用约束最小二乘滤波法进行退化图像复原;最后,对复原效果进行相应比较,结果表明在强湍流条件下本算法具有更为理想的复原效果。该研究可为复杂水流条件下水下退化图像复原方法研究提供参考。     

Soil Redistribution investigations by combined use of soil 137Cs and selected chemical properties

Abstract

To improve the methods of application of phosphorus or supply of soil P to Azolla (A. microphylld), basal application, split application, inoculation of P-enriched Azolla, and soil disturbance were compared. Soil disturbance did not increase the floodwater P content. Phosphorus was applied to inoculum production plots to enrich Azolla with P. Thus, phosphorus-enriched Azolla could multiply 5–7 times after inoculation until it became P deficient. Trials on the methods of enrichment of Azolla with P showed that the best method was to broadcast twice 4.33 kg P (10 kg P₂O₅/ha) at 2-day intervals and to harvest Azolla 3 days after P application. Addition of P once or twice 2 weeks after the inoculation of P-enriched Azolla further increased the biomass production. Efficiency of P application was analyzed in terms of N gain in relation to the amount of P applied. This ratio in the P-enriched Azolla treatment was higher than the economically sound ratio -5-, and higher than or equal to that in the standard split application.     

Biochar derived from dairy cattle carcasses as an alternative source of phosphorus and amendment for soil acidity

Two pot experiments were conducted to evaluate biochar derived from dead dairy cattle as a mineral fertilizer, especially phosphorus (P) fertilizer, and to clarify the effect of particle size of biochar on plant growth (Zea mays L.) and P uptake. To produce the biochar, body parts of dead cattle were placed in a charring chamber and allowed to char at 450°C for 4 h. The biochar was of high pH and rich in major plant nutrients, especially P. Application of fine biochar (< 1 mm) increased P uptake by the corn plants grown in soil of low available P status. As a result, plant growth was improved following biochar application and dry matter production was also increased. The effect of the biochar application on the P uptake and plant growth was promoted by the application of mineral nitrogen (N) fertilizer. Soil analysis after harvest indicated that the biochar application increased soil pH, available P and exchangeable calcium (Ca) and magnesium (Mg) compared with the soil before seeding, while soil available N and exchangeable potassium (K) were considerably decreased. The decrease in the soil available N was incomprehensible, because the result of the mass balance given by the difference between input as the applied N from the biochar and fertilizer N and output as the N uptake by the plants was positive. We observed a similar result in the mass balance of K to the case of N. The medium (2–4 mm) and coarse (> 4 mm) grade biochar did not significantly affect plant growth, because P uptake was not, or was only slightly, increased by the application of these biochars. Dissolution of P from the coarser biochars was probably slower than that of the fine biochar. The lower dissolution of P from the medium and coarse biochars was supported by the lower P absorption efficiency of these biochars compared with that of the fine biochar and superphosphate. The effect of fine biochar on plant growth and P uptake was similar to that of superphosphate. We can therefore conclude that fine biochar derived from cattle carcasses is an effective source of P fertilizer and amendment for soil acidity. The N and K contents in the biochar, although relatively high, cannot be relied upon as a mineral fertilizer. Further studies are needed to assess whether the N and K contents of the biochar indicate it can be regarded as a useful fertilizer.     

盛夏西太平洋副热带高压对陕西降水的影响

利用1961-2008年陕西78个气象站7-8月降水资料、西太平洋副高逐月指数资料及500hPa位势高度场和700hPa风场月平均再分析资料,通过相关、回归分析、小波凝聚谱分析等方法探讨盛夏西太平洋副热带高压对陕西降水的影响及机制,为盛夏陕西降水的气候预测提供依据.结果表明,盛夏副高强度、脊线、西伸脊点指数与陕西降水关系密切,副高偏强偏西时陕西降水偏多,副高偏北时陕西北部降水偏多、南部偏少.8月副高3个指数与陕西降水的相关关系更为密切,通过显著性检验的站点明显增多.副高的强度和位置变化引起副热带和中高纬度大气环流的异常,进而影响陕西降水.8月副高偏强偏西时,巴尔喀什湖阻高增强,700hPa西南季风的水汽输送增强,陕西位于冷暖空气交汇区,降水偏多;副高偏南时,水汽辐合区位于陕南,陕西南部多雨、北部少雨;副高偏北时,水汽辐合区位于陕北,陕西北部多雨、南部少雨.8月副高强度指数与同月陕西降水的显著共振周期为7 ～8a,且副高强度变化超前于陕西降水变化1.26a,说明8月副高强度的年际变化是引起同月陕西降水年际变化的重要原因.     

Promising approaches to the purification of soils and groundwater from hydrocarbons (A Review)

Soils and waters are affected by oil spills in the course of oil production and hydrocarbon leakages because of the corrosion of underground reservoirs, as well as the filtration of hydrocarbons from the tailing ponds formed during the extraction of oil from oil sands. The conventional technology for the withdrawal of contaminated water and its purification on the surface is low-efficient and expensive. New approaches are proposed for the in situ purification of soils and groundwater. To accelerate the oxidation, active substances atypical for the supergenesis zone are used: peroxides of metals and hydrogen. The efficiency of hydrogen peroxide significantly increases when the oxidation is catalyzed by Fe²⁺ or Fe³⁺ (Fenton reaction). The effects of Fe(III), sulfates, and carbon dioxide as electron acceptors are studied under anaerobic conditions (with oxygen deficit).