用表达人溶菌酶基因的重组质粒防治干乳期奶牛乳腺炎

将人溶菌酶（human lysozyme，hLYZ）cDNA插入由pcDNA3改造而成的pcDNAK表达载体。用获得的重组载体pcDNAKLYZ转染COS-1细胞，经免疫荧光试验证明能进行正确表达。将重组载体注射于哺乳母鼠，取其乳汁进行溶菌酶活性测定，结果显示，分泌在乳汁中的重组hLYZ高达139mg／L。根据乳汁体细胞检测结果，选择健康奶牛和乳腺炎阳性奶牛，分别在干奶时和产犊前2周2次注射重组质粒pcDNAKLYZ，注射途径为乳腺基部穿刺，注射剂量为300μg／乳区,于产犊后1个月采集奶样进行体细胞检测，结果显示，对前一泌乳期发生的奶牛乳腺炎的治愈率为91．5％，对下一泌乳期奶牛孔腺炎的预防有效率王少为96．97％。由此认为。构建的表达hLYZ基因的重组表达质粒，可以替代抗菌素类油乳剂用于干乳期乳腺炎的防治。     

鸡传染性喉气管炎及其防制研究

作者论述了鸡传染性喉气管炎的病原及培养特性、发病机制、诊断方法及其防制，并对传染性喉气管炎的控制和根除进行了展望。     

VEGF induces HUVECs to produce extracellular H2O2 and its proliferation role

AIM:To study the effect of VEGF on extracellular H₂O₂ production in HUVECs and the role of H₂O₂ in the VEGF-induced proliferation. METHODS:HUVECs was stimulated with 500 μg/L VEGF. Products of extracellular H₂O₂ was detected by H₂DCFDA staining. MTT method was used to value the influences of 3×10⁶ U/L catalase and 5-20 mmol/L H₂O₂ to VEGF function. RESULTS:After treatment for 15 min with VEGF, HUVECs appeared fluorescence, and continued to become stronger, peaked at 45 min then decreased. HUVECs, which was treated simultaneity with VEGF and 3×10⁶ U/L catalase, only appeared very faint fluorescence. The proliferation of HUVECs by VEGF was restrained when treated with 3×10⁶ U/L catalase. The extrinsic H₂O₂ at concentration of 5-10 mmol/L promoted the proliferation of HUVECs but inhibited the proliferation effect of VEGF on HUVECs (P<0.01). CONCLUSION:These findings indicate that VEGF induces HUVECs to produce extracellular H₂O₂ and plays role in proliferation, but extrinsic H₂O₂ restrains VEGF function.     

uclear factor κB in LPS stimulated rat alveolar macrophages promotes TNF-α secretion

AIM: To investigate the activation of nuclear factor κB (NF-κB) induced by lipopolysaccharides (LPS) in rat alveolar macrophages (AMs) and its regulatory role in tumor necrosis factor (TNF-α) secretion. METHODS: The dynamic activity changes of NF-κB induced by LPS were determined with electrophoretic mobility shift assay (EMSA). Antisense oligonucleotides of NF-κB subunit (p65) were transfected into AMs prior to LPS stimulation. The effect of antisense oligonucleotide transfection on expressions of p65 and TNF-α in supernatant were measured with Western blotting and enzyme linked immunosorbent assay (ELISA), respectively.RESULTS: NF-κB activity increased markedly and reached its peak level at 4 h after LPS stimulation. After transfected with antisense oligonucleotides of NF-κB subunit (p65), expression of p65 and TNF-α in supernatant decreased markedly.CONCLUSION: NF-κB activity has a positive effect on regulating secretion of TNF-α in AMs induced by LPS.     

家蚕微孢子虫(浙江株)α-微管蛋白基因部分片段的克隆及系统发育分析

基于微孢子虫分类学地位的争议,通过克隆家蚕微孢子虫(Nosema bombycis)的α-Tubulin基因,利用BioEditor将核苷酸序列翻译成氨基酸序列,并从NCBI中收集不同物种的α-Tubulin基因,用CLUSTALX(1.81)、Mega2以及在线生物信息学软件CLUSTALW(1.83)分析序列并构建系统发育树。结果显示:家蚕微孢子虫及其它微孢子虫与真菌聚为一类,且微孢子虫以一个独立群与接合菌(zygomycetes)的噬虫霉属(Entomophaga)、耳霉属(Conidiobolus)关系最近,与子囊菌(ascomycetes)、担子菌(basidiomycetes)、壶菌(chytrids)及其它接合菌互为姐妹群。     

新疆野生油菜芥酸含量的遗传分析

通过新疆野生油菜高、低芥校组合正反交Ｆ１、Ｆ２，回交一代及相应的杂交亲本种子中芥权的测定，分析了新疆野生油菜芥酸的遗传。结果表明，新疆野生油菜芥权酸含量受两对加性基因控制，每个芥权基因控制的芥量为５％左右。     

基于2D DWT与MobileNetV3融合的轻量级茶叶病害识别

针对现有茶叶病害识别方法病害信息挖掘不足导致识别准确率低的问题，该研究提出了一种基于二维离散小波变换(discrete wavelet transform, DWT)和MobileNetV3融合的茶叶病害识别模型CBAM-TealeafNet。为增强网络对病害频域特征的检测能力，将2D DWT获取的频域特征与bneck结构提取的深度特征融合，形成频域与深度特征融合的识别网络。为提高特征提取能力，在bneck结构中，嵌入卷积块注意模块 (convolutional block attention module, CBAM)，为特征通道分配相应权重。为解决样本类别不平衡对识别模型性能的影响，利用焦点损失函数取代交叉熵损失函数以提高识别精度。经验证，CBAM-TealeafNet在5种不同茶叶病害上整体识别准确率达到98.70%，参数量为3.16×10⁶，相对MobileNetV3，准确率提升2.15个百分点，参数量降低25.12%。该方法可为茶树叶部等作物病害轻量级识别研究提供模型参考。     

杉木树干材积分析

就杉木人工林立木树干材积的估测，材积按树高分布，材积的组成等有关问题进行了探讨。     

90年代江苏淮南小麦产量性状演变及品质分析

分析了江苏省淮南片 1 989～ 1 999年连续 1 0年 (5轮 ) 4 5个小麦品种 (系 )的区试结果 ,其平均产量为 5 .0 661 t.hm- 2 ;年度 (轮次 )间的产量呈现低 -高 -低的演变趋势 ;在产量构成因素中 ,穗数较为稳定 ,且与产量关系不密切 ;而每穗粒数随着产量的起伏表现较大增减 ,与产量呈显著正相关 ;千粒重与产量呈极显著正相关 ,粒重大小基本决定产量高低。本地区目前应用推广小麦品种的出粉率均较低 ;蛋白质和面筋含量以苏麦 6号较高 ,但由于其面团稳定时间短 ,仍不适于制作面包 ;扬麦 1 5 8和宁麦 8号适合制作面条和馒头 ;宁麦 9号为较优质的饼干、糕点小麦。此外 ,还探讨了小麦产量育种的策略和品质改良的重点及要求。