Influence of murine Toxocara canis infection on plasma and bronchoalveolar lavage fluid eosinophil numbers and its correlation with cytokine levels

Toxocara canis is a nematode of the Ascaridae family that normally parasites the small intestine of canid species. Humans are accidentally infected upon ingestion of embryonated eggs, and can manifest several clinical alterations such as fever, hepatomegaly, splenomegaly, respiratory symptoms, muscle pain and anorexia. In the present work, we investigated the kinetics of tissue distribution of L2 larva in lungs, liver, kidney, brain, skeletal muscle and myocardium. Also, we analyzed the blood and bronchoalveolar lavage fluid (BAL) for levels of IL-6, IFN-gamma, eotaxin and Regulated on Activation Normal T Cell Expressed and Secreted (RANTES) in experimental murine T. canis infection. We observed liver, lung and kidney lesions correlated to larva migration as early as the first day of infection. After the seventh post-infection day, larva could also be detected in brain, skeletal muscle and heart, as an indicator of biphasic migration pattern. Increased inflammatory activity was detected in BAL and plasma of infected animals, as was an intense eosinophil migration associated with an increase in the levels of all the cytokines studied. In conclusion, our results establish a tight correlation between tissue lesions caused by larva migration and increased plasma levels of pro-inflammatory and eosinophil chemotactic cytokines. Thus, murine T. canis infection may prove to be useful in understanding the role of cytokines in infection.     

猪流行性腹泻病毒实时荧光定量RT-PCR检测方法的建立

根据Gen Bank报道的N基因高度保守核苷酸序列,设计并合成一对引物。上下游引物与Gen Bank中登录的153株猪流行性腹泻病毒(PEDV)N基因全长序列匹配度分别是100%和97%。以本实验室分离流行毒株为模板,利用SYBR Green I荧光染料法进行RT-PCR扩增,获得扩增产物构建重组质粒作为阳性对照,建立检测猪流行性腹泻病毒核酸的方法。同一样品进行3次重复试验,变异系数0.9%。通过对临床样品进行检测和测序验证,核酸检测结果中的阳性样品准确率为100%。本研究所建立的荧光定量PCR检测方法具有快速、灵敏、准确等优点,可用于临床PEDV的检测及分子流行病学调查。     

Pharmacokinetics of oxytetracycline in hemolymph from the Chinese mitten crab, Eriocheir sinensis

Feng, Q., Wu, G. H., Liang, T. M., Ji, H. Y., Jiang, X. J., Gu, W., Wang, W. Pharmacokinetics of oxytetracycline in hemolymph from the Chinese mitten crab, Eriocheir sinensis. J. vet. Pharmacol. Therap. 34 , 51–57. The purpose of this study was to investigate the hemolymph kinetics and depuration time of oxytetracycline following intramuscular administration at doses of 2, 8 and 40 mg/kg body weight, respectively. The concentration of OTC in hemolymph was assayed using solid phase extraction and high performance liquid chromatography. The elimination half‐life of the terminal part of the elimination phase (t_1/2β) ranged from 87.9 to 114.3 h. The total body clearance (CL_b) was 0.0430 L/kg/h at the lower dose, 0.0123 L/kg/h at the medium dose and 0.0013 L/kg/h at the higher dose. The apparent volume of the central compartment (V_c) was found to be 1.383, 0.699 and 0.143 L/kg respectively. The depuration time for each dose was 13.6, 29.6 and 57.6 days, respectively. Results from the present study suggest that the 40 mg/kg dose might have the best therapeutic efficacy following intramuscular administration.     

遮荫胁迫下外源一氧化氮对高羊茅的生理调控作用

用外源一氧化氮(NO)供体硝普钠(SNP)处理遮荫胁迫下的2个高羊茅(Festuca arundinacea)品种,探讨外源NO对遮荫胁迫下其叶绿素含量、膜透性、丙二醛(MDA)含量、抗氧化酶活性及脯氨酸(Pro)含量的影响.结果表明:外源NO可以显著提高遮荫胁迫下高羊茅叶片的叶绿素含量(P＜0.05),减弱质膜相对透性的增加,减缓膜质过氧化产物MDA含量的升高,促进Pro的积累,提高超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性(P＜0.05).遮荫胁迫下高羊茅品种踏火二号的电解质渗透率、MDA含量、CAT活性和Pro含量的变化显著(P＜0.05),NO的作用效果     

动物产品中硒富集的研究

<正>硒具有抗癌、抗氧化、抗心血管疾病等功效,因此富集硒的动物产品受到了越来越多的关注。饲料中添加的硒源主要有无机硒(亚硒酸钠、纳米硒)和有机硒(酵母硒、蛋氨酸硒),不同硒源在动物组织中的沉积量差异很大。Behne等(1983)用含0.3mg/kg硒的日粮饲喂兔子,各组织硒含量从高到低依次为肾脏、肝脏、     

欧盟皮内变态反应比较试验法结合ELISA检测牛结核病的研究

现在应用较多的牛结核病检疫方法有变态反应、ELISA等方法。检疫牛结核病目前OIE唯一指定方法是皮内变态反应,该方法也是最古老的方法之一,经过多次改进,仍然为各国所用。该方法的不足是非特异性反应过高,且只能检测细胞免疫状态。牛结核     

Computer image analysis of intramuscular adipocytes and marbling in the longissimus muscle of cattle

The deposition of fat in muscle, recognized by the consumer as marbling, is an important meat quality trait. The objective of the study was to provide additional insights into the quantitative extent of marbling by means of computer image analysis. Fifty-one F(2) generation German Holstein and Charolais crossbreed cattle, 18 mo of age, were used to determine relationships among marbling traits, adipocyte size, and the amount of adipose tissue in different depots. Differences were recorded among the size of i.m. adipocytes in different groups of marbling flecks, divided according to the location in the muscle cross-section and to the size of the marbling flecks. The results showed positive correlation between i.m. adipocyte size and the weight of s.c. fat, intestinal fat, omental fat, and perirenal fat (r = 0.50, 0.61, 0.70, and 0.63, respectively, P < 0.001). The i.m. adipocyte size was correlated with i.m. fat content, number of marbling flecks, proportion of marbling fleck area, and total length of marbling flecks (r = 0.71, 0.44, 0.62, and 0.55, respectively, P < 0.01). The number of marbling flecks was also correlated with i.m. fat content, proportion of marbling fleck area, and total length of marbling flecks (r = 0.58, 0.62, and 0.91, P < 0.01, respectively). The ventral marbling flecks had a 5-fold larger fleck area, 4-fold more adipocytes, and larger adipocytes (P < 0.001). Larger marbling flecks contained larger adipocytes (P < 0.001). Moreover, compared with the small marbling flecks, there was a 48-fold larger fleck area and 26-fold more adipocytes in the large marbling flecks. The results indicate that i.m. fat deposition increases concurrently with the other fat depots but is still independent. Furthermore, the i.m. fat is preferentially deposited in the ventral area of LM. Although the i.m. adipocyte size has an important effect on the traits of marbling flecks, cell number plays a greater role in i.m. fat deposition than cell size.     

草原蝗虫密度的抽样技术研究

在蝗虫密度为０．０５～８．５０头／ｍ２的不同类型草地，以投环方式统计不同大小（０．０２、０．０５、０．１０、０．２０、０．３５ｍ２）的铁环击中或套入蝗虫数，计算调查地蝗虫平均数，以笼罩法作为“真值”对照，对不同大小铁环调查数进行统计检验。结果表明，在调查密度范围内，以０．０５、０．１０、０．２０ｍ２铁环的调查数较接近“真值”，其精确性随蝗虫密度的高低略有变动。根据草地蝗虫为Ｐｏｉｓｓｏｎ分布的特点，采用Ｐｏｉｓｓｏｎ置信域和二项式抽样法，建立以０．１０ｍ２环中蝗虫的出现率估测平均密度和反估计的二项式抽样模型，并进行统计检验。最后，以Ｐｏｉｓｓｏｎ置信值为概率保证，提出了理论抽样数计算模型。     

鸽新城疫病

鸽新城疫(ND)是由新城疫病毒(NDV)引起鸽等家禽发生的一种急性、败血性、高度接触性传染性疾病,主要表现为呼吸系统、消化系统和神经系统的综合症状,如呼吸困难、下痢、神经机能紊乱等。由于该病与鸡新城疫病属同由一种RNA病毒引起,相互之间可以发生交叉感染,加上鸽的流动性大,所以鸽新城疫的发病率和死亡率都极高,以幼鸽为主的鸽群发病率可达100%,死亡率可达90～95%,常给养鸽者和其它家禽养殖者造成重大经济损失。是近几年来多发的一种家禽传染病。鸽新城疫(ND)以幼鸽最易感,但成年鸽的感受性同样比较高,死亡率也较大。病鸽、带毒的健…