近红外光谱变量筛选提高西瓜糖度预测模型精度

水果的内部品质是水果分级、保鲜及存储的一项重要指标,利用近红外光谱技术对西瓜内部品质进行快速无损检测研究有着非常重要的意义。为了研究变量筛选方法对西瓜糖度预测模型精度的影响,该文以麒麟瓜为研究对象,利用近红外漫透射光谱技术对麒麟瓜可溶性固形物含量（SSC）进行检测,采用偏最小二乘回归（PLSR）,多元线性回归（MLR）和主成分回归（PCR）建立麒麟瓜可溶性固形物数学模型,并探讨等间隔平均光谱和等间隔抽取光谱变量筛选结合连续投影算法（SPA）对预测模型精度的影响。研究结果表明：光谱经等间隔抽取（间隔5,115个变量）经归一化预处理,结合SPA优选出6个波长建立的PLSR预测模型的相关系数（rpre）为0.828、校正均方根误差（RMSEC）为0.589、预测均方根误差（RMSEP）为0.611。该模型预测效果相对较优,建模时间短,提高了模型的预测能力和预测精度。该研究为西瓜内部品质的在线无损检测提供研究基础。     

Agronomic traits of soybean cultivars released in different decades after grafting record‐yield cultivar as rootstock

Breeders have seldom considered the selection for root traits during the genetic improvement in soybean. It is hypothesized that grain yield would be increased by the root function improvement, especially for the current cultivars. The objective of this grafting experiment was to determine the effect of record‐yield cultivars L14 or Z35 as rootstocks on agronomic traits of cultivars released in different decades. A total of 11 cultivars, released in different decades, were used to graft onto L14 or Z35 rootstocks. The agronomic traits were measured in the pot‐culture experiments. Grafting cultivars released in different decades onto L14 or Z35 rootstocks resulted in higher yield, 100‐seed mass and apparent harvest index as compared with those of non‐grafted or self‐grafted plants. Grain yield gain of cultivars grafted onto record‐yield cultivar rootstocks was 0.40 g/plant/year from 1966 to 2006, which was larger than that of non‐grafts and self‐grafts (0.27 g/plant/year). The yield of current cultivars should increase more if their root functions were improved.     

Uneven ripening in ‘Bangalore blue’ grape (Vitis vinifera × Vitis labrusca) berries in India is linked to seed viability

Uneven ripening (UR) is a physiological disorder of unknown origin in ‘Bangalore blue’ grape (Vitis vinifera × Vitis labrusca) leading to wine of inferior quality. A preliminary study found wide variations in total dehydrogenase activity (TDH) of seeds from unevenly ripe berries. In our experiments, gibberellin (GA₃) applied to young grapes increased seed TDH activity and reduced the incidence of uneven ripening to 2% compared with 35% in the control. In contrast, paclobutrazol (PBZ) decreased TDH activity and increased the incidence of the disorder to 58%. GA₃-treated berries had higher concentrations of sucrose and TDH activity in seed representing mature seeds with high viability. In contrast, PBZ-treated and control berries had higher concentrations of glucose and lower TDH activity, indicating immature seeds with low viability. These results suggested that competition among developing berries can lead to differences in seed gibberellin content, seed viability and the rate of berry growth resulting in green, purple, and black berries at harvest. The study established the role of seed viability in uneven ripening and demonstrated that the incidence of the disorder is reduced by the application of GA₃ to immature berries.     

基于ADAMS的油茶果采摘机械臂试验设计及优化

针对目前我国油茶采摘主要以人工为主,劳动强度大、采摘效率低等问题,设计了一种适合园艺化栽培的履带式油茶果采摘机,并在Adams软件中对机械臂进行试验设计和优化仿真。试验数据表明:油茶果采摘机工作区间与各机械臂的长度和液压缸安装距离呈线性相关,最佳组合处的机械臂长度总和减小25.67%,可为油茶果采摘机的机械臂设计提供参考。     

切-双纵流脱分装置试验研究与分析

阐述了切-双纵轴流脱粒分离装置的结构组成及工作原理,基于切-双纵流联合收获机,以喂入量、切流滚筒间隙及滚筒转速为影响因子,脱粒破碎率及脱粒损失率为指标,进行田间性能试验,并利用极差分析分别对破碎率、脱粒损失率单影响因子进行分析。结果表明:对脱粒破碎率影响的主次因素为BCA,即切流滚筒转速纵轴流滚筒转速喂入量,最佳组合为切流滚筒间隙25/30mm、滚筒转速907/1 043r/min、喂入量10.2 kg/s;对脱粒损失率影响的主次因素为BAC,即切流滚筒间隙喂入量滚筒转速,最佳组合为切流滚筒间隙25/30mm、喂入量10.2kg/s、滚筒转速953/1 096r/min。     

苹果图像的背景分割与目标提取

水果的缺陷、大小和颜色差异以及光照等因素影响图像背景分割与目标提取精度.以苹果为研究对象,针对4种不同光照强度条件下采集的280幅不同姿态的苹果图像,将彩色图像的R、G、B分量进行算术运算,然后采用形态学开运算进行消噪处理,采用线性空间滤波消除锯齿状边界,采用自动阈值分割方法进行背景分割与目标提取.结果显示,203幅图像的分割偏差小于1％,占总量的72.5％;70幅图像的分割偏差大于1％而小于2％,占总量的25％;偏差大于2％的有7幅,占总量的2.5％.最大分割偏差为2.83％.     

Interaction of curcumin with phosphatidylcholine: A spectrofluorometric study

Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3, 5-dione], the main constituent of the rhizomes of the plant Curcume longa L. (turmeric), is a powerful antioxidant in both enzymatic and nonenzymatic systems. The interactions of curcumin with egg and soy phosphatidylcholine were followed by fluorescence spectroscopy. Curcumin had very weak fluorescence in aqueous system, which was enhanced in apolar environments. Curcumin emitted at 490 nm after being excited at 451 nm in phosphatidylcholine micelles. The equilibrium constants for the interaction of curcumin with egg and soy phosphatidylcholine were (3.26 +/- 0.2) x 10(5) and (2.64 +/- 0.2) x 10(5) M(-1), respectively. From the Scatchard plot of the fluorometric data, it was inferred that one molecule of curcumin could bind six molecules of phosphatidylcholine. The equilibrium constant for the phosphatidylcholine-curcumin interaction decreased with temperature, indicating the amphiphilic nature of curcumin. The DeltaG, DeltaH, and DeltaS values obtained for the interaction of egg phosphatidylcholine-curcumin were -7.8 +/- 0.3 kcal/mol, -9.6 +/- 0.4 kcal/mol, and -6.8 +/- 0.2 cal/mol/K, respectively. The fluorescence anisotropy measurements of curcumin with phosphatidylcholine suggested that the anisotropy of the curcumin molecule did not change in phosphatidylcholine. The interaction of divalent metal ions with phosphatidylcholine-curcumin in comparison with phosphatidylcholine-1-anilino-8-naphathalenesulfonic acid complex suggested the strong binding of curcumin to metal ions.     

Response of the pigeonpea-Rhizobium symbiosis to salinity stress: variation among Rhizobium strains in symbiotic ability

Summary There were significant differences among pigeonpea [Cajanus cajan (L.) Millsp] Rhizobium sp. strains (IC 3506, IC 3484, IC 3195, and IC 3087) in their ability to nodulate and fix N₂ under saline conditions. Pigeonpea plants inoculated with IC 3087 and IC 3506 were less affected in growth by salinity levels of 6 and 8 dS m^-1 than plants inoculated with the other strains. For IC 3506, IC 3484, and IC 3195, there was a decrease in the number of nodules with increasing salinity, while the average nodule dry weight and the specific nitrogenase activity remained unaffected. However, in IC 3087, the number of nodules increased slightly with increasing salinity. Leaf-P concentrations increased with salinity in the inoculated plants irrespective of the Rhizobium sp. strain, and leaf-N concentrations decreased with increasing salinity in IC 3484 and IC 3195 only. Shoot-Na and-Cl levels were further increased in these salt-sensitive strains only at 8 dS m^-1. Therefore there may be scope for selecting pigeonpea Rhizobium sp. symbioses better adapted to saline conditions. The Rhizobium sp. strains best able to form effective symbioses at high salinity levels are not necessarily derived from saline soils.Submitted as JA No. 919 by the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)     

干旱地区喷洒水利用系数的田间试验研究

为了科学地评价喷灌在干旱地区的适宜性,在内蒙古包头春小麦生育期内对喷洒水利用系数进行了监测,结果指出,通过选择适宜的灌溉时间,喷洒水利用系数可以达到0.85以上。对影响喷洒水利用系数的环境因素进行分析后得出,风速的影响最大,相对湿度次之,气温的影响很小;在此基础上建立了喷洒水利用系数与上述3因子之间的回归模型。为了估算整个灌溉季节的喷洒水利用系数,对所研究地区1991～2001年灌溉季节(4～9月份)内的日平均风速进行了统计分析,发现不大于3m/s的日数占灌溉季节总日数的90%以上,因此,选择日平均风速不大于3m/s的时间灌溉可以满足作物的需水要求,这种情况下,整个灌溉季节的喷洒水利用系数可以达到0.83。由此可见,在条件与包头类似的干旱地区,从提高水的利用率的角度出发,发展喷灌也是适宜的     

Proton toxicity interferes with the screening of common bean (Phaseolus vulgaris L.) genotypes for aluminium resistance in nutrient solution

Common bean (Phaseolus vulgaris L.) proved to be very sensitive of low pH (4.3), with large genotypic differences in proton sensitivity. Therefore, proton toxicity did not allow the screening of common bean genotypes for aluminium (Al) resistance using the established protocol for maize (0.5 mM CaCl₂, 8 μM H₃BO₃, pH 4.3). Increasing the pH to 4.5, the Ca²⁺ concentration to 5 mM, and addition of 0.5 mM KCl fully prevented proton toxicity in 28 tested genotypes and allowed to identify differences in Al resistance using the inhibition of root elongation by 20 μM Al supply for 36 h as parameter of Al injury. As in maize, Al treatment induced callose formation in root apices of common bean. Aluminium‐induced callose formation well reflected the effect of Ca supply on Al sensitivity as revealed by root‐growth inhibition. Aluminum‐induced callose formation in root apices of 28 bean genotypes differing in Al resistance after 36 h Al treatment was positively correlated to Al‐induced inhibition of root elongation and Al contents in the root apices. However, the relationship was less close than previously reported for maize. Also, after 12 h Al treatment, callose formation and Al contents in root apices did not reflect differences in Al resistance between two contrasting genotypes, indicating a different mode of the expression of Al toxicity and regulation of Al resistance in common bean than in maize.