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131.
Nolen-Walston R Bedenice D Rodriguez C Rushton S Bright A Fecteau ME Short D Majdalany R Tewari D Pedersen D Kiupel M Maes R Del Piero F 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(4):846-852
BACKGROUND: Eastern equine encephalitis (EEE) virus is a mosquito-borne togavirus (alphavirus) that causes severe (often fatal) encephalitis in many mammalian species, but it has not been reported previously in South American camelids. Hypothesis: South American camelids can become naturally infected with EEE virus and show encephalitic signs similar to those observed in other affected species. ANIMALS: Nine cases (8 alpacas and 1 llama, aged 3.5 weeks to 12 years) were identified; 4 of 9 were 510 weeks old. All cases were from the East Coast of the United States and presented in late summer and fall. METHODS: A retrospective study was performed to include confirmed cases of EEE in camelids in North America before 2006. RESULTS: Eight of nine (89%) camelids died or were euthanized in extremis, with the mean time to death of 2 days. Clinical signs were consistent with encephalitis and included fever, lethargy, ataxia, seizures, recumbency, torticollis, opisthotonus, and vestibular signs. No consistent hematologic abnormalities were identified, and cerebrospinal fluid contained an increased protein concentration in the single camelid analyzed. No successful therapy was identified. EEE was confirmed by alphavirus detection by using immunohistochemistry (IHC) and polymerase chain reaction (PCR) in the central nervous system (CNS) and by serology. Findings included polioencephalitis with lymphocytic perivascular cuffing; neutrophil infiltration; gliosis; neuron satellitosis; necrosis; and edema, with intracytoplasmic alphavirus within neurons and glial cells. No virus was detected in extraneural tissues. CONCLUSIONS AND CLINICAL IMPORTANCE: In endemic areas, EEE should be considered a differential diagnosis for young and adult camelids with CNS disease. Brain histopathology with indirect IHC or PCR is diagnostic. 相似文献
132.
The following three articles have been published online as part of the EVE Oncology Virtual Issue which is available online at wileyonlinelibrary.com/journal/eve 相似文献
133.
Saba A Mazzini F Raffaelli A Mattei A Salvadori P 《Journal of agricultural and food chemistry》2005,53(12):4867-4872
The olive oil market is suffering from sophisticated illegal treatments. One common adulteration process consists of the addition to virgin olive oil of lower quality oils, such as "lampante" oil, an inexpensive oil and with some organoleptic defects, which is then submitted to thermal deodorization under vacuum processes for removal of the undesired flavor components. Such a blending may not have a huge influence on the chemical composition and may not significantly affect the parameters usually checked as quality indicators, although the organoleptic properties may change. As a consequence, a major effort is being devoted to find reliable markers able to unmask such adulterations. We report here the complete characterization of a compound, detected at trace levels exclusively in thermal stressed oils, which could be a candidate marker for adulteration. The investigation, carried out by GC-MS and GC-MS/MS, provided its complete structure, including the stereochemistry, shown to be a 9(E),11(E)-18:2 fatty acid methyl ester. Experimental data also confirmed the influence of both temperature and heating time on formation and concentration of this compound. 相似文献
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135.
Luigi Milella Danilo Saluzzi Mauro Lapelosa Giuseppe Bertino Piero Spada Ivana Greco Giuseppe Martelli 《Genetic Resources and Crop Evolution》2006,53(8):1715-1720
Random-amplified polymorphic DNA (RAPD) markers were used to evaluate genetic variability among populations of an Italian
strawberry ecotype, and to determinate genetic relationships between genotypes and their putative ancestor. A total of 65
selections and one cultivar ‘Madame Moutot’ (MM), were analysed to evaluate genetic variability present in Etna mountain area
and to confirm as MM was one of the cultivars that originated the ecotype. A total of 222 RAPD markers was obtained using
16 decamer primers and 6 longer primers, 90.8% of the markers obtained by selected primers resulted polymorphic at least within
analysed genotypes. RAPDs were used to calculate genetic similarity coefficients and to generate dendrograms representing
genetic relationships among genotypes analysed. Cluster analysis displays as RAPD polymorphisms were able to characterize
the genotype variability among closely related groups. The data show as MM could be considered the ancestral genotypes introduced
in that area. The results obtained confirm that RAPD markers could be used as reliable markers to perform phylogenetic studies
in Fragaria×ananassa Duch. ex Rozier.
Giuseppe Bertino and Piero Spada - Coauthor involved in genotype selection and field management 相似文献
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138.
da Silva Reginaldo Barboza Iori Piero Tavares Rose Luiza Moraes de Souza Zigomar Menezes de Lima Camila Cassante de Melo Silva Francisca Alcivânia de Souza Bento Marília 《Precision Agriculture》2022,23(3):984-1007
Precision Agriculture - Changes in land use in riverside ecosystems added to the flood and ebb regimes of rivers modify the spatial variability of the physical–mechanical attributes of soil,... 相似文献