谷物冷却机处理偏高水分粮安全度夏试验

利用谷物冷却机向偏高水分粮试验仓房的空间输入经过调控的冷气,使储粮平均粮温全年处于准低温状态,有效抑制储粮害虫的发生和延缓储粮品质陈化速度,充分保证中央储备粮安全度夏。     

甜菜分子标记InDel-PCR引物的筛选

为了筛选出适合甜菜分子标记的InDel引物以及利用InDel引物构建能够扩增多重InDelPCR反应的引物,以提高甜菜InDel-PCR扩增的效率,利用8个甜菜品种的DNA对300对甜菜InDel引物进行筛选,结果筛选出多态性较好、条带清晰、易于识别的甜菜InDel引物44对,这44对引物共扩增出总条带116条,其中多态性条带109条,多态性比率为94%;其中有37对引物扩增的总条带为2或3条,可以作为将来多重InDel引物的首选;又从中筛选出7对多重InDel引物:ND228、ND31、ND267、ND229、ND66、ND231、ND22。     

缺硼条件下苎麻器官解剖学研究

应用光镜和电镜观察比较了缺硼和适量供硼条件下苎麻器官的解剖学。结果:缺硼苎麻花粉粒空瘪,小孢子母细胞减数分裂不正常;植株的分生组织和输导组织的发育受抑制;叶片不正常加厚,栅栏薄壁细胞内含物呈丝状解体,叶柄皮层薄壁细胞中有较多的淀积物。     

水稻产量形成与叶片含氮量及叶色的关系

以 6个中晚熟水稻品种为材料 ,研究了不同时期叶片含氮量与分蘖发生、穗粒形成及产量的关系。结果表明 ,分蘖发生率随出生时叶片含氮量提高而提高 ,分蘖发生与终止的临界叶片含氮量随分蘖出生期推迟而逐步提高 ;穗颖花数主要受倒 2叶期、倒 1叶期叶片氮状况影响 ,结实率与抽穗期叶片氮状况有关 ,穗颖花数和结实率最高时叶片含氮量粳稻为 2 7～ 2 8g·kg-1,籼稻为 2 5g·kg-1左右。试验发现 ,N -n叶龄期、倒 2叶期和抽穗期主茎顶 4叶叶色接近或略深于顶 3叶为不同品种高产的共同特征 ,并提出了高产水稻适宜的叶片含氮量指标     

Changes in the riparian zone of the lower Eygues River,France, since 1830

The riparian forests along braided rivers are dynamic, frequently rejuvenated by floods and channel changes, and thus dominated by pioneer to middle stages of succession; they are sites of high biodiversity in some regions. The Lower Eygues River (drainage area 1150 km² in southeastern France) is such a braided river system with large alluvial forests dominated by Salix alba, Populus alba, and P. nigra. It was identified as a site of ecological interest by the EU under the Natura 2000 program. Such forests elsewhere in Europe have been identified as reference ecosystems. We documented the historical evolution of this alluvial forest from detailed (1:2500 scale) early 19th C parcel maps, early 20th C topographic maps, aerial photography from 1947 to 1996, and field surveys of topography and riparian vegetation in 1997–1998. Our results show that in 1830, the channel was wide, aggraded, and agricultural pressure extended literally to the channel edge. With decline in the rural population and reduced agricultural and grazing pressure in the catchment, erosion rates declined. Reduced sediment supply led to channel narrowing and incision. This channel narrowing, coupled with reduced agricultural pressure along the banks, has allowed riparian forest to colonize former active channel areas, especially within late 19th-century 20th century flood dykes. In recent decades, aggregate mining, and clearing for recreation and agriculture have fragmented the forest. Thus, the alluvial forest of the Lower Eygues is largely an artifact of changing human land-use over the past century, a context that should frame efforts for preservation and restoration.     

流通领域动物防疫监督管理工作的探讨

随着畜牧业飞速发展,流通领域的动物防疫监督管理工作在近年来也得到了迅猛发展,同时陆续暴露了一些问题,这些问题严重影响了动物防疫工作的进一步开展。为此,笔者结合工作实践,分析流通领域动物防疫监督管理工作中存在的突出问题,并探讨解决问题的对策。1存在的问题1.1束缚监督管理工作开展因素多。一是执法人员对法律精神领会不够,对法律条文学习不透。一些基层动物防疫监督执法人员一直认为:依据动物防疫法第四十一条,对市场和运输环节未经检疫的动物和动物产品的监督结果就是补检、加倍收取检疫费,只有当未经检疫的动物、动物产品检疫不…     

新疆野生花卉资源植物调查及引种栽培的初步研究

本文在较全面调查的基础上介绍了新疆境内主要野生观赏资源植物的分布概况。通过前期所做的有关野生花卉种子生活力、发芽率和解除体眠等方面的实验，筛选出8种较宜引科的野生花卉植物进行引种栽培试验。最后探讨了野生花卉植物引种栽培的应用前景。     

Progression of Coxiella burnetii infection after implementing a two-year vaccination program in a naturally infected dairy cattle herd

Background

The high prevalence of Coxiella burnetii infection in dairy cattle herds recently reported and the long survival time of the bacterium in the environment pose a risk to human and animal health that calls for the implementation of control measures at herd level. This study presents the results of a 2-year vaccination program with an inactivated phase I vaccine in a Spanish dairy herd naturally infected with C. burnetii. Calves older than 3 months and non-pregnant heifers and cows were vaccinated in April 2011 and the farm was subsequently visited at a monthly basis for vaccination of recently calved cows and calves that reached the age of 3 months. Annual booster doses were given to previous vaccinated animals as well. The effectiveness of the vaccine was assessed in terms of level of C. burnetii shedding through milk and uterine fluids and environmental contamination as determined by polymerase chain reaction (PCR).

Results

The percentage of shedder animals through uterine fluids and milk progressively decreased, and C. burnetii DNA load in bulk-tank milk samples was low at the end of the study. The average seroconversion rate in not yet vaccinated animals, which acted as control group, was 8.6% during the first year and 0% in the second year. DNA of C. burnetii was found in aerosols and dust samples taken in the calving area only at the beginning of the study, whereas slurry samples remained C. burnetii PCR positive for at least 18 months. Multiple Locus Variable number tandem-repeat Analysis identified the same genotype in all C. burnetii DNA positive samples.

Conclusions

In the absence of any changes in biosecurity, the overall reduction of C. burnetii infection in animals to 1.2% milk shedders and the reduced environment contamination found at the end of the study was ascribed to the effects of vaccination together with the culling of milk shedders. Vaccination has to be planned as a medium-long term strategy to suppress risks of re-infection.