Histopathological and immunohistochemical findings of primary and metastatic medullary thyroid carcinoma in a young dog

This report describes the gross, histological, and immunohistochemical features of medullary thyroid carcinoma (MTC) with pulmonary metastases in a young dog. Sheets of pleomorphic cells supported by fibrous stroma characterized the primary mass, while metastatic nodules had a neuroendocrine pattern. Despite differing histologic features, all masses showed marked immunoreactivity against calcitonin and multiple neuroendocrine markers consistent with MTC. Although MTC is a well-recognized entity, it may be difficult to distinguish this mass from other thyroid neoplasms, necessitating immunohistochemical characterization.     

银离子对鱼类及其他水生动物的毒性

以白鲢(鱼种),罗非鱼(鱼种)、鲤(鱼种)、鲫(成鱼)、中华绒螯蟹(幼体)、克氏螯虾(成体)、蝌蚪等水生动物为材料,做银的毒性试验,同时分析含银水体中,鱼体内的银富集状况。试验结果表明:(1)银离子对这几种水生动物幼体的半致死浓度(LC_(50))仅在2—40ppb之间;但对成鱼的 LC_(50)要比幼体大得多。(2)在含银水体中,底层鱼类(如鲫)的银富集量最大的部位在内脏;上层鱼类(如?条)富集量最大的部位是鳃。鱼类中肌肉的富集量最小,其次是骨。     

两系籼型杂交水稻源库特征的研究

对籼型两系与三系杂交水稻源库特征的比较研究结果表明：齐穗后水稻群体的高效叶面积指数（LAI）,矫正光合势（APP）等源特征指标值及总库容量,籽粒充实度,势容比,群体干物质生产量,产量在组合间差异达显著或极显著水平,两系和三系中各有指标值高低的组合;其主要差别在组合间,而不在两系和三系两种类型之间,两系杂交末有明显的优势。两系籼型杂交稻的发展应充分两系法的优势,不仅要选配总库容量大,千粒重高的组合,更要选配齐穗后高效LAI,APP,CGR等源特征值高的组合,齐穗后有较强的干物质生产能力。在栽培措施上可针对籼型两系杂交稻不同组合的源库特点进行调控。     

丹江口湖北库区马尾松人工林水文生态效应

采用野外实地观测与室内浸水法,对丹江口湖北库区龙口林场5种类型马尾松林分枯落物蓄水、土壤理化性质和蓄水能力、地表径流和土壤侵蚀等水文效应进行了研究。结果表明：①不同类型马尾松林枯落物层最大持水量间存在显著差异,变化范围在45．83-93．32t·hm^-2,为松柏混交林〉针阔混交林〉低密度马尾松林〉中密度马尾松林〉高密度马尾松林。有效拦蓄量变化范围在29．83-41．79t·hm^-2,为中密度马尾松林〉松柏混交林〉针阔混交林〉低密度马尾松林〉高密度马尾松林。②随着土壤深度的增加,土壤容重呈增加的趋势,而总孔隙度呈逐渐递减的趋势。不同类型马尾松土壤贮水能力差异明显,表现为针阔混交林〉低密度马尾松林〉中密度马尾松林〉松柏混交林〉高密度马尾松林,针阔混交林约是高密度马尾松林的1．25倍。③不同森林类型地表径流量在14．68-29．56m^3·hm^-2之间,土壤侵蚀量在2．01～4．15m3·hm^-2之间,且均以栎类次生林最优,松柏混交林次之,马尾松纯林最低。总体而言,综合利用抚育择伐和人工诱导自然更新的方法,人工诱导马尾松纯林逐步向针阔混交林发育,可更有效提高水土保持功能。     

Early life administration of milk fat globule membrane promoted SCFA-producing bacteria colonization,intestinal barriers and growth performance of neonatal piglets

Milk fat globule membrane (MFGM) possesses various nutritional and biological benefits for mammals, whereas its effects on neonatal gut microbiota and barrier integrity remained unclear. This study investigated the effects of MFGM administration on microbial compositions and intestinal barrier functions of neonatal piglets. Sixteen newborn piglets were randomly allocated into a CON group or MFGM group, orally administered with saline or MFGM solution (1 g/kg body weight) respectively during the first postnatal week, and all piglets were breastfed during the whole neonatal period. The present study found that the MFGM oral administration during the first postnatal week increased the plasma immunoglobulin (Ig) G level, body weight and average daily gain of piglets (P < 0.05) on 21 d. Additionally, MFGM administration enriched fecal SCFA-producing bacteria (Ruminococaceae_UCG-002, Ruminococaceae_UCG-010, Ruminococaceae_UCG-004, Ruminococaceae_UCG-014 and [Ruminococcus]_gauvrearuii_group), SCFA concentrations (acetate, propionate and butyrate; P < 0.05) and their receptor (G-protein coupled receptor 41, GPR41). Furthermore, MFGM administration promoted intestinal villus morphology (P < 0.05) and barrier functions by upregulating genes of tight junctions (E-cadherin, claudin-1, occludin and zonula occludin 1 [ZO-1]), mucins (mucin-13 and mucin-20) and interleukin (IL)-22 (P < 0.05). Positive correlation was found between the beneficial microbes and SCFA levels pairwise with the intestinal barrier genes (P < 0.05). In conclusion, orally administrating MFGM during the first postnatal week stimulated SCFA-producing bacteria colonization and SCFA generation, enhanced intestinal barrier functions and consequently improved growth performance of neonatal piglets on 21 d. Our findings will provide new insights about MFGM intervention for microbial colonization and intestinal development of neonates during their early life.     

硼对油菜不同品种酶活性和根系活力的影响

在土培和水培条件下,研究硼对不同芥菜型油菜品种某些酶活性和根系参数的差异。结果表明,缺硼使叶片中SOD、CAT活性降低,而使POD和G6PDHase增加;敏感品种的缺硼与否差异显著,而不敏感品种则差异不明显。缺硼对敏感品种根系长度、重量、体积、表面积、活跃吸收面积以及比活跃表面积的抑制作用也明显大于不敏感品种,因而影响养分的吸收和代谢。     

Diversity and phylogenetic analysis in Cocos nucifera L. in Mexico

The diversity and phylogenetic analysis in 22 populations of Mexican coconut and six imported populations, were estimated using 15 enzymatic systems and the allele frequencies in: peroxidase (PER), endopeptidase (ENP), and glucose 6-Phosphate dehydrogenase (G6PD). There was very low polymorphism, not more than two alleles per locus. The Wright fixation indexes F(it) = 0.62, F(is) = 0.40, and F(st) = 0.36 indicated low total heterosygosity and low heterosygosity within populations suggesting endogamy and genetic drift, and a high diversity among populations due to differentiation between Pacific and Gulf of Mexico coastal populations. The phylogenetic tree with values for genetic distance, indicated three groups on the Pacific coast related to Rennell Tall and Polynesian Tall, and two groups on the coast of the Gulf of Mexico, one related to West African Tall and another related to Mexican Pacific coast populations. This corroborates historical antecedents and morphological and physiological patterns. The Dwarf coconuts related to the Pacific Tall populations, Rennell Tall and Polynesian Tall. There was no difference between local and imported Dwarf populations.     

土壤硼不同化学库特性研究──Ⅰ．热水溶性硼的植物有效性

用湖北省５种旱地土壤进行了土壤热水溶性硼（ＨＷＳＢ）植物有效性试验。盆栽试验结果表明，除ＨＷＳＢ较高的潮土外，其余４种土壤施硼均有不同程度的增产效果。盆栽试验和培养实验都证明热水不能完全提取当季施入土壤中的硼，而这些硼在后季作物生长中继续发挥效果。尽管５种土壤的ＨＷＳＢ与盆栽作物的增产幅度有一定的相关性，但以质地较轻、缺硼严重的供试砂质灰紫色土效果最明显。从而说明土壤硼对植物的有效性与影响土壤硼库活性的因素有关。     

农业高新技术的发展领域

农业高新技术是以生态经济学的理论,综合运用现代农业和现代生物农业工程等科技成果,构成一个多学科的信息与技术相结合,使技术、经济、生态协调统一,具有高产、高效、优质、低耗和生态良性循环的现代集约化农业技术体系.本文主要介绍了农业高新技术的主要发展领域.     

蛋鸡H5、H9亚型禽流感和新城疫免疫血清抗体与卵黄抗体检测比较分析

为分析蛋鸡免疫后卵黄抗体与血清抗体的相关性,试验采集经H5、H9亚型禽流感和新城疫疫苗免疫的黔东南小香鸡、乌蒙乌骨鸡、威宁鸡血清样本和卵黄样本,采用血凝抑制试验进行相关免疫抗体检测。结果:3个地方品种鸡的血清样本和卵黄样本中均可检测出H5、H9亚型禽流感和新城疫免疫抗体,且几何平均效价均大于24以上,达到免疫合格标准,不同地方品种鸡群间差异不显著(P>0.05);免疫后30d,卵黄样本免疫抗体几何平均效价低于血清样本,但差异不显著(P>0.05);免疫后60d,卵黄样本免疫抗体几何平均效价低于血清样本,差异显著(P<0.05)。结果表明:卵黄抗体与血清抗体呈正相关性,卵黄抗体几何平均效价均低于血清抗体。在临床上可用卵黄抗体替代血清抗体进行H5、H9亚型禽流感和新城疫疫苗免疫效果的评价,但应选择在免疫后30d以内。