A high-resolution radiation hybrid map of the human genome draft sequence

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.     

蛋白质与淀粉对挂面和方便面品质及微观结构的影响

以小麦淀粉、小麦活性面筋粉和标准面粉为原料 ,通过控制各原料比例的方法 ,结合扫描电子显微镜技术 ,观察与分析了不同淀粉与蛋白质含量的混合粉在制作面条和方便面过程中微观结构、加工特性及食用品质的变化情况。结果表明 ,蛋白质和淀粉含量对面条和方便面的品质都有重要影响 ,相对而言 ,蛋白质的作用大于淀粉。只有蛋白质和淀粉间比例及其在面粉中含量处于比较合适的水平时 ,才能制成高品质的挂面和方便面。蛋白质经吸水或变性后形成的网络结构是使面团具有可塑性、熟面条具有弹性和部分韧性、方便面具有良好复水性的主要原因 ;也是束缚淀粉颗粒 ,防止其从面条中失落的主要因素。淀粉通过缓解面筋强度、添补蛋白质网络空隙等途径 ,有增大面团和面带延伸性、改善面带表面光滑性、增加面条白度等作用。     

历史文学的真实性和吸烟习尚

历史文学作为一种特殊的文学样式 ,应在体现历史真实的前提下进行合理地想象、艺术加工和创造 ,不违背历史常理和历史常识。从吸烟习尚在中国的形成看 ,烟草自 17世纪的万历年间才陆续传入我国 ,而长篇历史小说《红瘦》和历史话剧《关汉卿》等有关历史文学作品 ,却将其写进了所着力表现的某历史社会生活中 ,这似乎有悖于历史文学的特质和创作原理     

我国农村纠纷多元化解决机制探析

多元化纠纷解决机制是我国重要的社会治理方式,在解决农村矛盾纠纷的过程中发挥着不可替代的作用。多元化纠纷解决机制能够在我国广大的农村地区存在并发展,因其有着重要的现实基础。目前,多元化纠纷解决机制在司法的权威和公信力、各纠纷解决机制关系和功能发挥等方面存在诸多问题,严重制约了多元化纠纷解决机制的作用在农村的发挥。因此,有必要在坚持多元化与利益协调理念相统一、人本与保护农民权益理念相统一、法治和自治理念相统一的前提下,从不同的方面对我国现行多元化纠纷解决机制予以完善。     

硅酸盐细菌代谢产物对植物生长的促进作用

从根际微生物的角度研究了硅酸盐细菌对植物生长的促进作用。代谢产物对植物生长促进作用最好的是１３８＃菌,处理５０天,水稻株高比对照增加２７．７％,生物量比对照增加４０．２％,硅酸盐细菌的代谢产物对植物生长的刺激作用与浓度有关,低浓度的代谢产物对植物生长有明显的促进作用,高浓度的代谢产物则有一定的抑制作用,代谢产物的作用对根系生长的影响尤为明显。     

荷叶离褶伞菌丝最佳生长条件研究

本文研究了菌丝生长所需要的碳源、氮源、无机盐、pH值以及离褶伞子实体浸出液对荷叶离褶伞菌丝生长的影响。结果表明,荷叶离褶伞菌丝生长最适碳源是葡萄糖,最适氮源是蛋白胨,每1000mL培养基中加入1g磷酸二氢钾或1g氯化钙能促进菌丝生长;菌丝在pH值5—9都能生长,但在酸性环境下生长较好;加入离褶伞子实体漫出液对荷叶离褶伞菌丝生长促进作用明显。     

辽宁瓦房店北部5乡镇土壤质量变异研究

运用地统计学,结合GIS分析了辽宁瓦房店北部5乡镇土壤表层（0=20 cm）有机质、碱解氮、速效磷、及速效钾的空间变异规律及其驱动机制。通过对瓦房店北部5乡镇209个采样点养分含量及其半方差分析和Kriging插值的研究结果表明：该地区有机质均值为8.9358 g/kg,碱解氮均值为65.6744mg/kg,速效磷均值为52.3676 mg/kg,速效钾均值为58.4863 mg/kg。与第二次土壤普查资料相比,有机质含量略有下降,速效磷含量上升非常多,速效钾含量也略有上升。变异系数大小依次为速效磷、速效钾、碱解氮、有机质,分别为53.04%、42.52%、42.32%、29.24%,都属于中等变异。半方差分析的结果表明,瓦房店北部地区土壤养分各采样点的相关系数都在0.25-0.75之间,空间相关性中等。人类的施肥习惯、耕作措施的改变及土地利用变化是引起土壤养分变化的主要原因。     

A comparison of crop data measured by two commercial sensors for variable-rate nitrogen application

Nitrogen (N) fertilizer rates applied spatially according to crop requirements can improve the efficiency of N use. The study compares the performance of two commercial sensors, the Yara N-Sensor/FieldScan (Yara International ASA, Germany) and the GreenSeeker (NTech Industries Inc., Ukiah, California, USA), for assessing the status of N in spring wheat (Triticum aestivum L.) and corn (Zea mays L.). Four experiments were conducted at different locations in Quebec and Ontario, Canada. The normalized difference vegetation index (NDVI) was determined with the two sensors at specific growth stages. The NDVI values derived from Yara N-Sensor/FieldScan correlated with those from GreenSeeker, but only at the early growth stages, where the NDVI values varied from 0.2 to 0.6. Both sensors were capable of describing the N condition of the crop or variation in the stand, but each sensor had its own sensitivity characteristics. It follows that the algorithms developed with one sensor for variable-rate N application cannot be transferred directly to another sensor. The Yara N-Sensor/FieldScan views the crop at an oblique angle over the rows and detects more biomass per unit of soil surface compared to the GreenSeeker with its nadir (top-down) view of the crop. The Yara N-Sensor/FieldScan should be used before growth stage V5 for corn during the season if NDVI is used to derive crop N requirements. GreenSeeker performed well where NDVI values were >0.5. However, unlike GreenSeeker, the Yara N-Sensor/FieldScan can also record spectral information from wavebands other than red and near infrared, and more vegetation indices can be derived that might relate better to N status than NDVI.     

吉燕3号的选育及栽培技术

介绍燕麦新品种吉增3号的选育经过、主要的特征特性及其高产栽培技术,以期为该品种推广提供参考。     

应用盐溶蛋白电泳图谱鉴定玉米种子纯度

应用盐溶蛋白电泳方法对38份玉米杂交种和37份亲本自交系进行了蛋白电泳生化指纹图谱品种真实性及其纯度检测。结果表明：有34份单交种能够很好地鉴定出来，4份单交种未能鉴定出来，鉴定有效率达89％；本方法还能鉴定亲本自交系的纯度及同一母本的不同单交种，能有效监控假冒种子。对凝胶谱带胶板进行干燥处理，从根本上解决了该项技术结果保存难的关键问题，仅用3－4d就能准确建立品种的特征谱带样板，为该项技术的规范化应用和进一步研究奠定了基础。该方法具有操作简便、快速、准确、经济、可批量检测的特点，适合在实际中应用。