Changes in the immunostaining intensities of follicle-stimulating hormone and luteinizing hormone during ovarian maturation in the female Japanese flounder

The role of gonadotropin (GTH) in the reproduction of the Japanese flounder, Paralichthys olivaceus, was studied by assessing the changes in the apparent activity of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the pituitary gland during gonadal maturation by immunohistochemical analyses. Corresponding changes in plasma levels of testosterone (T), estradiol-17β (E₂), and 17α,20β-dihydroxy-4–pregnen-3-one (DHP) were also studied. Reared fish at the early spawning to termination stages were sampled from May to August and wild fish at the previtellogenic to termination stages were caught at 3- to 4-week intervals between April and September offshore from the northern mainland of Japan by gill nets. The gonadosomatic index of the reared fish decreased from the early spawning stage to the termination stage, while that of the wild fish increased significantly from the previtellogenic stage to the early spawning stage and decreased thereafter. In the reared fish, the immunostaining intensities of FSH and LH were high during the spawning period, accompanied by high plasma levels of T, E₂, and DHP. In the wild fish, the immunostaining intensities of FSH and LH were low during the previtellogenic stage but increased during the maturing and spawning stages. These results indicate that both FSH and LH are likely associated with oocyte maturation in the Japanese flounder.     

VHSV IVb infection and autophagy modulation in the rainbow trout gill epithelial cell line RTgill-W1

Autophagy modulation influences the success of intracellular pathogens, and an understanding of the mechanisms involved might offer practical options to reduce the impact of infectious disease. Viral haemorrhagic septicaemia virus (VHSV) can cause high mortality and economic loss in some commercial fish species. VHSV IVb was used to infect a rainbow trout gill cell line, RTgill-W1, followed by the treatment of the cells with different autophagy-modulating reagents. LC3II protein using Western blot was significantly (p < .05) decreased for two days following VHSV infection, and immunofluorescence confirmed that LC3II-positive intracytoplasmic puncta were also decreased. Infection with VHSV resulted in significantly decreased expression of the autophagy-related (Atg) genes atg4, at12, atg13 and becn1 after one day using quantitative PCR. Both viral gene copy number and VHSV N protein were significantly decreased by treating the cells with autophagy-blocking (chloroquine) and autophagy-inhibiting reagents (deoxynivalenol and 3-methyladenine) after three days, while autophagy induction (restricted nutrition and rapamycin) had limited effect. Only treatment of RTgill-W1 with deoxynivalenol resulted in a significant increase in expression of type I interferon. Therefore, the suppression of autophagy initially occurs after VHSV IVb infection, but the modulation of autophagy can also inhibit VHSV IVb infection in RTgill-W1 after three days.     

Agrotourism and fast urbanisation: The double pressure of development on peri-urban agriculture in Hôi An,a small city of central Vietnam

Agrotourism in Vietnam has been identified as one of the strategies used to achieve green growth and countryside modernisation, and it is often included as part of the national and local agenda. In this paper, we examine agrotourism in a village in the periphery of Hội An city (an international tourism hub in central Vietnam) to question tourism's interaction with ongoing development processes. More specifically, we aim to understand the impact of fast peri-urbanisation on agrotourism and the impacts of agrotourism on people's daily lives, specifically when it comes to physical changes in their living environment, tensions in their social life and their concerns about the future. Our analysis is supported with data generated from interviews with farmers, local officials, tourism workers and tourists. We find that agrotourism products lacked authenticity and farming was not of great interest for tourists, yet the state's investment in the village tended to favour spaces and infrastructure that could attract more tourists and generate profit, to the detriment of cultural infrastructure. Land speculation and an unequal distribution of income were the main tensions in the village along with farmers' concerns about their rural heritage, income diversification and environmental quality. As such, agrotourism in the village has been driven by rapid urbanisation and mass tourism, creating a competition between a consumption activity and a productive activity. Those are important parameters that future policymakers need to take into consideration in order to sustain the city's food production and tourism.     

Effects of replacing fish meal with soya protein concentrate on growth,feed efficiency and digestibility in diets for snakehead,Channa striata

Soya bean meal‐based formulated feeds have recently become available for snakehead culture in Vietnam. This study was conducted to determine the appropriate replacement of fish meal (FM) protein by another soya product, soya protein concentrate (SPC), in snakehead (Channa striata) diets. Five iso‐nitrogenous (45% crude protein) and iso‐caloric (19 KJ g^?1) practical diets were formulated to replace 0% (control), 40%, 60%, 80% and 100% of protein FM by protein SPC (100% FM, 40% SPC, 60% SPC, 80% SPC and 100% SPC respectively). A digestibility experiment was also conducted with the same formulated diets with addition of 1% chromic oxide. Fish fed 100% FM and 40% SPC diets had significantly better growth and survival compared with other treatments. Feed intake, feed conversion ratio, protein efficiency ratio and net protein utilization, trypsin and chymotrypsin activities of experimental fish fed 100% FM and 40% SPC diets were significantly higher than those fed other diets. The apparent digestibility coefficient (ADC) of the diet and diet components, ADC_diet, ADC_protein and ADC_lipid, of fish fed diet 40% SPC and 100% FM treatment were significantly higher than those of other treatments. The cost/kg fish produced in diets 100% FM and 40% SPC was much lower compared with other treatments. Dietary inclusion levels of SPC in diet above 40% significantly affected fish survival, growth, digestibility and trypsin and chymotrypsin activities, although fish chemical composition was not greatly affected.     

Sensory and Physicochemical Properties of Smoked Catfish Sausages

The objective of this study was to determine the physiochemical characteristics and sensory quality of smoked, coarse-ground catfish sausages and catfish frankfurters by using 100% fillets, 75% fillets with 25% nuggets, and 50% fillets with 50% nuggets (the proportions of fillets and nuggets equaled 100% for each of the three formulations). Pork fat was added to each formulation to bring the final fat content to 20%. Lightness (L*) decreased (p < 0.05) as percentage of nuggets increased in frankfurter and coarse-ground sausages. Based on texture profile analysis results, coarse-ground sausages showed lower (p < 0.05) springiness, cohesiveness, hardness, gumminess, and chewiness than frankfurters. Attributes of aroma, flavor, and texture were not affected (p > 0.05) by catfish fillet percentage but were affected (p < 0.05) by sausage type. In addition, no differences existed (p > 0.05) in consumer acceptability among frankfurter samples, with all samples receiving average scores between “like moderately” and “like very much.” Results indicate that catfish fillets and nuggets could be used to produce catfish frankfurters with acceptable sensory properties.     

基因工程菌枯草芽孢杆菌GEB3产生的脂肽类抗生素及其生物活性研究

 利用MALDI TOF MS技术 ,鉴定了将lpaB3基因转入枯草芽孢杆菌Bacillussubtilis 168菌株所构建的工程菌GEB3产生的脂肽类抗生素种类。结果表明 ,GEB3仅产生表面活性素 (surfactin) 1种脂肽类抗生素。经LC MS分析 ,GEB3产生由 13、14和 15个碳原子的脂肪酸链构成的标准表面活性素变异体 (standardsurfactinisoforms)。生物活性检测表明 ,该工程菌产生的脂肽类抗生素表面活性素具有抑制小麦纹枯病菌和稻瘟病菌菌丝生长的作用     

Trace Metal Contamination During Grinding of Plant Samples

Inductively coupled plasma-mass spectroscopic (ICP-MS) analysis of leaves from 22 cabbage crops in the Sa P? and B?c Hà districts of Láo Cai Province, North-Western Vi?t Nam, revealed unexpectedly high concentrations of chromium (Cr) and nickel (Ni). The concentrations were strongly linearly related (r² = 0.94), indicating sample contamination during grinding through a stainless-steel hammer mill. We tested this hypothesis in two ways. First, brown rice ground through the same mill was contaminated not only by Cr and Ni, but also cobalt (Co), iron (Fe) and molybdenum (Mo). Second, scanning electron microscopy/energy dispersive analysis of x-rays (SEM/EDS) of the ground samples revealed small fragments with co-located Fe, Cr and Ni, consistent with stainless steel wear fragments. Other grinders may perform differently and we suggest that quality assurance protocols for trace metal analysis of plants should include testing for grinder wear metals. Lastly, brown rice appears to be convenient for investigating contamination of plant tissues during grinding.