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151.
Summary Two hundred clones were randomly selected in 1988 and in 1989 from a hybrid population ofSolanum phureja andSolanum stenotomum to determine the relationship between specific gravity (SG) and percent dry matter (%DM), and between tissue specific gravity
(TSG) and %DM. The objectives were: (1) to determine the dry matter prediction equation for a diploid potato population, (2)
to determine whether estimates of %DM and efficiency of selection for %DM could be improved by measuring TSG, and (3) to determine
the influence of year on the estimates. In 1988 the prediction equation for the relationship between %DM and SG was %DM=198.71
(SG-191.30,r=0.85. Ranges and means for the variables were: (1) %DM: 16.99–32.67, mean 25.16; (2) SG: 1.054–1.122, mean 1.089. In 1989
the relationship of %DM and SG was predicted as %DM=194.46(SG)-187.53,r=0.87; whereas the relationship of %DM and TSG was predicted as %DM=196.77(TSG)-192.91,r=0.87. Ranges and means for the variables were: (1) %DM: 10.68–34.57, mean 21.32; (2) SG: 1.030–1.122, mean 1.074; (3) TSG:
1.046–1.141, mean 1.089. Slopes were not significantly different between any of the prediction equations, but intercepts were
significantly different. Estimates of %DM were not improved by measuring TSG. Year did influence the relationship between
%DM and SG. 相似文献
152.
Little is known about the decomposition rates of shoot and root residues of perennial grasses. This knowledge is important to estimate the carbon sequestration potential of the grasses. An incubation experiment was carried out in a sandy clay loam with shoot and root residues of three native perennial grasses (Wallaby grass, Stipa sp. and Kangaroo grass) and the annual grass barley either separately or in mixtures of two residues. Respiration rate was measured over 18 days, and microbial C and available N were measured on days 0 and 18. Decomposition was lower for roots than for shoots and lower for residues of perennial grasses than for barley. Cumulative respiration was positively correlated with water-soluble C in the residues but not with residue C/N. In the mixtures, the measured cumulative respiration was higher than the expected value in five of the nine mixes usually where the differences in cumulative respiration between the individual residues were relatively small. Lower than expected cumulative respiration were found in two of the mixtures in which barley shoots (high cumulative respiration) were mixed with residues with low cumulative respiration. There was a negative correlation between the change in microbial biomass C concentration from day 0 to day 18 and cumulative respiration on day 18. In the amended soils, the available N concentration decreased from day 0 to day 18. It is concluded that the low decomposition rate of perennial grasses residues should favour C sequestration, but that mixing residues of similar decomposition rate may accelerate their decomposition. 相似文献
153.
154.
Phosphorus (P) can be added to soil as inorganic P or crop‐residue P, but little is known about how these two forms of P addition affect soil P pools and how their effect changes with the rate of P addition. A glasshouse experiment was conducted to assess the effect of inorganic P and P added as residues at different rates on (1) soil P pools at two time points: immediately after amendment and 42 d later, and (2) growth and P uptake by wheat at flowering (day 42). Three types of legume residues (faba bean young shoot, chickpea mature shoots with pods, and white lupin mature shoots without pods) were added to a loamy‐sand soil at a rate of 5 or 15 g residue kg–1. Inorganic P was added at four different rates (3, 10, 30, and 100 mg P kg–1) to give P‐addition rates corresponding to the total P added with the different residues at the two residue rates. Soil P pool concentrations (microbial P, resin‐P, NaHCO3‐P, NaOH‐P, HCl‐P, and residual P) and wheat growth and P uptake (shoot and root) were measured after 6 weeks. Compared to inorganic P addition, P added with residues led to a 10%–80% greater increase in shoot biomass at the two highest P‐addition rates. Wheat P uptake was positively correlated with resin‐P and microbial‐P concentrations in residue‐P‐amended soil, but with resin‐P and NaOH‐Pi concentrations in soil amended with inorganic P. The concentration of HCl‐P decreased by up to 30% from day 0 to day 42 in the residue treatments and that of residual P decreased by about 20% in all treatments during this period suggesting that these nonlabile P pools are quite dynamic and could serve as P source for plants. 相似文献
155.
Respiratory chlamydial infection based on experimental aerosol challenge of pigs with Chlamydia suis
Sachse K Grossmann E Berndt A Schütt C Henning K Theegarten D Anhenn O Reinhold P 《Comparative immunology, microbiology and infectious diseases》2004,27(1):7-23
An experimental study of aerogeneous challenge in pigs was conducted in order to reveal characteristic features of porcine respiratory chlamydiosis. Eight conventionally raised pigs were exposed to a pathogenic strain of Chlamydia (C.) suis, four controls were mock infected. Besides pathological changes, the acute-phase and humoral immune responses, as well as the dissemination and transmission of the challenge strain was monitored in the course of infection. The data from clinical investigations, LPS-binding protein assay, antibody ELISAs, confocal laser scanning and light microscopy, immunohistochemical staining and PCR provided extensive evidence of the pathogenic potential of C. suis for the porcine respiratory system. This model appears suitable for further pathophysiological and immunological investigations of chlamydial respiratory infections and can also be recommended for studies of Chlamydia-associated infections of the human lung. 相似文献
156.
Petra Ondrackova Lenka Leva Zdenka Kucerova Monika Vicenova Marketa Mensikova Martin Faldyna 《Veterinary research》2013,44(1):98
Monocytes play an essential role in the defense against bacterial pathogens. Bone marrow (BM) and peripheral blood (PB) monocytes in pigs consist of the main “steady-state” subpopulations: CD14hi/CD163-/SLA-DR- and CD14low/CD163+/SLA-DR+. During inflammation, the subpopulation of “inflammatory” monocytes expressing very high levels of CD163, but lacking the SLA-DR molecule (being CD14low/CD163+/SLA-DR-) appears in the BM and PB and replaces the CD14low/CD163+/SLA-DR+ subpopulation. However, current knowledge of monocyte migration into inflamed tissues in pigs is limited. The aim of the present study was to evaluate the distribution of “inflammatory” CD14low/CD163+/SLA-DR- monocytes during experimental inflammation induced by Actinobacillus pleuropneumoniae (APP) and a possible role for chemokines in attracting “inflammatory” CD14low/CD163+/SLA-DR- monocytes into the tissues. Monocyte subpopulations were detected by flow cytometry. Chemokines and chemokine receptors were detected by RT-qPCR. The “steady-state” monocytes were found in the BM, PB, spleen and lungs of control pigs. After APP-infection, “inflammatory” monocytes replaced the “steady-state” subpopulation in BM, PB, spleen and moreover, they appeared in an unaffected area, demarcation zone and necrotic area of the lungs and in tracheobronchial lymph nodes. They did not appear in mesenteric lymph nodes. Levels of mRNA for various chemokines with their appropriate receptors were found to be elevated in BM (CCL3-CCR1/CCR5, CCL8-CCR2/CCR5, CCL19-CCR7), necrotic area of the lungs (CCL3-CCR1, CCL5-CCR1/CCR3, CCL11-CCR3, CCL22/CCR4) and tracheobronchial lymph nodes (CCL3-CCR1) and therefore they could play a role in attracting monocytes into inflamed tissues. In conclusion, “inflammatory” monocytes appear in different lymphoid tissues and the lungs after APP infection in pigs. Various chemokines could drive this process. 相似文献
157.
Jolanta Klukowska‐Rötzler Ludovic Chervet Eliane J. Müller Petra Roosje Eliane Marti Jozef Janda 《Veterinary dermatology》2013,24(1):54-e14
Background – In humans, thymic stromal lymphopoietin (TSLP) plays a central role in the development of allergic inflammation, such as atopic dermatitis (AD), but it is unknown whether it is involved in the pathogenesis of canine AD (CAD). Hypothesis/Objectives – Our aim was to characterize canine TSLP and to assess its expression in CAD. Methods – Canine TSLP was identified based on sequence homology with human TSLP and the complementary DNA (cDNA) cloned by RT‐PCR. Real‐time quantitative RT‐PCR was established to assess the expression of canine TSLP in cultured canine keratinocytes and in skin biopsy specimens from lesional and nonlesional skin of 12 dogs with CAD and eight healthy control dogs. Results – Partial canine TSLP cDNA was cloned and characterized. It contained four exons that shared 70 and 73% nucleotide identity with human and equine TSLP, respectively, encoding the signal peptide and full‐length secreted protein. We found significantly increased TSLP expression in lesional and nonlesional skin of dogs with CAD compared with healthy control dogs (P < 0.05), whereas no difference was measured between lesional and nonlesional samples. In cultured primary canine keratinocytes, we found increased TSLP expression after stimulation with house dust mite allergen extract or Toll‐like receptor ligands lipopolysaccharide and poly I:C. Conclusions and clinical importance – Increased TSLP expression in the skin of dogs with CAD supports an involvement of TSLP in the pathogenesis of CAD similar to that in humans. Further studies should elucidate the function and therapeutic potential of TSLP in CAD. 相似文献
158.
Background – The management of atopic dermatitis (AD) in dogs relies mainly on the use of interventions to reduce pruritus and skin lesions. Objectives – To provide a critical analysis of recent clinical trials reporting the efficacy and safety of interventions for canine AD. Methods – Systematic review of randomized controlled trials (RCTs) published, presented or completed between 2008 and 2011, which enrolled dogs with AD. The search was done using electronic databases, reviewing published meeting abstracts and sending queries to professional email lists. Trials reporting the efficacy of interventions aimed at treating, preventing or reducing glucocorticoid usage in atopic dogs were selected. Results – Twenty‐one RCTs were included. We found further moderate‐quality evidence of efficacy and safety of oral glucocorticoids and ciclosporin for treatment of canine AD. There was additional moderate‐quality evidence of the efficacy of a topical glucocorticoid spray containing hydrocortisone aceponate. Low‐quality evidence was found for the efficacy and safety of injectable recombinant interferons, a budesonide leave‐on conditioner, a ciclosporin topical nano‐emulsion and oral fexofenadine. There is low‐quality evidence of efficacy of oral masitinib, with a need for monitoring for protein‐losing nephropathy. Finally, we uncovered low‐quality evidence of efficacy of a commercial diet as a glucocorticoid‐sparing intervention and of a glucocorticoid spray as a flare‐delaying measure. Very low‐quality evidence was found for the efficacy of other interventions. Conclusions and clinical importance – Topical or oral glucocorticoids and oral ciclosporin remain the interventions with highest evidence for efficacy and relative safety for treatment of canine AD. 相似文献
159.
Emma M. Johansson Petra M.A. Fransson Patrick A.W. van Hees 《Soil biology & biochemistry》2009,41(6):1111-1116
Despite its potential impact on soil carbon flow, few studies have attempted to quantify the effects of elevated carbon dioxide (CO2) on production of exudates by mycorrhizal plants. In this study we quantified low molecular weight (LMW) organic compounds exuded by non-mycorrhizal (NM) and ectomycorrhizal (ECM) plants in relation to exposure to elevated CO2. Scots pine seedlings, either colonized by one of eight different ECM fungi or non-mycorrhizal (NM), were exposed to either ambient (350 ppm) or elevated (700 ppm) concentrations of CO2. Exudation of LMW organic acids (LMWOAs), amino acids, dissolved monosaccharides and total dissolved organic carbon (DOC) was determined and exudation rates were calculated per g root and fungal dry mass. CO2 had a significant impact on exudation. Under elevated CO2, exudation of total LMWOAs increased by 120-160%, amino acids by 250%, dissolved monosaccharides by 130-270% and DOC by 180-220% compared to ambient CO2 treatment. Net CO2 assimilation rates increased significantly by 41-47% for seedlings exposed to elevated CO2. Exuded C calculated as a percentage of assimilated CO2 increased by 41-88% in the elevated CO2 treatment compared to ambient CO2 treatment. 相似文献
160.