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81.
Abstract The present review summarizes the available data on the concentration of trace elements (TEs) in soils and their availability to plants with a view to reflect the quality and safety of food and fodder crops. Most soils in western Balkan countries are not contaminated. However, soils around industrial centers and historical mining sites do create concern for food and feed safety. Also high concentrations of TEs are related to their geochemical original. For example, ultrabasic rocks and serpentinites in western Serbia and western Bosnia are very rich in chromium, nickel, magnesium, iron (Fe), and cobalt, and cover an area of 5200 km2. High TE concentrations caused by anthropogenic activities are also reported. In vineyard soils in Croatia, concentrations of cadmium, copper (Cu), and zinc (Zn) were much higher than their background concentrations. On the other hand, TE deficiency in plants is also prevalent in the regions. For example, Zn and Fe deficiencies in eastern parts of Croatia and northern parts of Serbia, Cu deficiency in pasture and sheep's blood at Nisici Plateau of Bosnia and Herzegovina, and selenium (Se) deficiency almost in the whole region have been observed. Therefore, information on TEs' behavior and soil factors affecting their mobility and availability is highly needed in order to separate the areas of contamination and then propose agrotechnical measures to protect the entry of TEs into the food chain. Research is also required to assess the influence of agronomic management on TE supply to plants and for achieving a better utilization of essential TEs. Concentrations of Se in wheat in Serbia are so low that if people were fed exclusively with wheat, their daily requirements for Se would not be met. There is also a need for full implementation of new food safety regulations in the Balkan countries in accordance with the legislations of the European Union. 相似文献
82.
M. Horvatić M. Grüner B. Vajić 《Plant foods for human nutrition (Dordrecht, Netherlands)》1977,27(2):177-185
Changes in the contents of main aminoacids have been watched during the period of wheat ripening. The aminoacids have been determined by microbiological methods. The results obtained have been calculated according to the statistics used in British Pharmacopeia. Reliability range has been settled for each results separately. Determination fault amounts to ±4,6% on an average. The total aminoacids have been calculated to 16% nitrogen. According to analysis results the aminoacids can be classified in four groups, i.e.:
- Aminoacids which share in total proteins remain constant during wheat ripening: Threonine, Valine, Isoleucine.
- Aminoacids which share in total proteins decrease during wheat ripening: Lysine.
- Aminoacids which share in total proteins increase during wheat ripening: Methionine, Arginine, Tryptophane.
- Aminoacids whose contents show no regularity during the wheat ripening: Leucine, Histidine, Phenylalanine.
83.
Ivermectin is one of the most commonly used drugs in pharmacotherapy of parasitic diseases in domestic and wild animals caused
by parasitic nematodes and arthropods. However, ivermectin and other avermectins very often produce side-effects in hosts.
The most dominant clinical symptom of ivermectin toxicity in domestic and wild animals is CNS depression. In nematodes, the
target site of ivermectin’s action is glutamate-gated chloride-channel receptor and GABA receptor. The depressive effect of
ivermectin in mammals might include more than one mechanism; therefore, the anticonvulsive effect of ivermectin against convulsions
caused by lidocaine and strychnine was evaluated. Ivermectin antagonized lidocaine- and strychnine-induced convulsions in
rats, although these have different mechanisms. In the present study, the anticonvulsive ED50 of ivermectin for lidocaine-induced convulsions was 2.44 mg/kg (95% CL 1.67 to 3.57 mg/kg), whereas for convulsions induced
by strychnine it was higher at 4.25 mg/kg (95% CL 2.32 to 3.78 mg/kg). At the same time, both anticonvulsive doses are significantly
lower then the observed LD50 of ivermectin (18.20 mg/kg). Furthermore, flumazenil (0.1 and 0.2 mg/kg), an antagonist of benzodiazepine receptors, antagonizes
just one part of these anticonvulsive effects of ivermectin. Our results show the significant anticonvulsive properties of
ivermectin and support the findings that ivermectin in the CNS of mammals produces multiple inhibitory effects, probably through
participation in the function of GABA-sensitive and GABA-insensitive chloride channels. 相似文献
84.
Gill Reaction to Pollutants from the Tamiš River in Three Freshwater Fish Species,Esox lucius L. 1758, Sander lucioperca (L. 1758) and Silurus glanis L. 1758: A Comparative Study 下载免费PDF全文
J. Lujić M. Matavulj V. Poleksić B. Rašković Z. Marinović D. Kostić B. Miljanović 《Anatomia, histologia, embryologia》2015,44(2):128-137
The study evaluated the effects of waterborne pollutants from the Tami? River on gill histology and possible differences in gill reaction patterns between three freshwater fish species, pike Esox lucius L. 1758, pike‐perch Sander lucioperca (L. 1758) and wels catfish Silurus glanis L. 1758 from the Tami? River. Gills from analysed fish species showed moderate to intense histopathological alterations. The most frequent progressive alteration was hyperplasia of epithelium, whereas the most frequent regressive alteration was epithelial lifting. Circulatory disturbances were most often manifested in the form of hyperaemia. During comparative analysis, differences in gill indices, reaction and alteration indices, as well as in gill and filament prevalence between analysed species, were observed. Although all analysed fish species did show both progressive and regressive alterations, there was a significant difference in the level of expression of these reaction patterns. Gill index obtained for pike clearly stands out as the lowest. Wels catfish showed the highest progressive reaction index, significantly higher in comparison with the other two species (P < 0.05), while pike‐perch showed the highest regressive reaction index, also significantly higher in comparison with the other species (P < 0.001). These results may implicate species‐specific gill reactions and thus present a useful tool for better understanding toxic mechanisms of various pollutants. 相似文献
85.
86.
With the intention of using the pig as a large animal model in haematopoietic research, a clonal assay in methylcellulose was developed and the optimal conditions for raising erythroid progenitors from adult pig bone marrow (BM) and peripheral blood (PB) have been established. Progenitor cells were stimulated to proliferate and differentiate in vitro by growth factors containing leucocyte condition medium (LCM), and with recombinant human erythropoietin (rhEpo). The number of PB BFU-E (burst forming units - erythroid) directly depended on the concentration of LCM, but BM BFU-E were not dependent on LCM. Both CFU-E (colony forming units - erythroid) and BFU-E were rhEpo dependent. Despite relatively high but expected individual variations, the mean number of colonies, as well as the functional characteristics of progenitor cells investigated, were similar to those of miniature pigs and some other mammals. 相似文献
87.
Terzić S Jemersić L Lojkić M Madić J Grom J Toplak I Sver L Valpotić I 《Veterinary research communications》2003,27(4):329-339
Ten pigs, aged 85 days, were vaccinated with a subunit vaccine containing 32 g of classical swine fever virus glycoprotein E2 (gp E2) (group 1), and a further 10 pigs were vaccinated with a C strain vaccine (104±0.15 TCID50/ml), produced by amplification in minipig kidney (MPK) cell culture (group 2). Nine non-vaccinated pigs served as a control group (group 3). Serum samples were collected before (day 0) and at 4, 10, 21 and 28 days after vaccination and were analysed by two commercially available enzyme immunoassays and by a neutralizing peroxidase-linked assay (NPLA). At the same times, peripheral blood was taken for determining the total leukocyte count and the body temperature was taken daily. Antibodies were not detected in serum samples collected before vaccination (day 0), and no side-effects that could be connected with vaccination were observed during the trial. Ten days after vaccination 6/10 pigs vaccinated with the subunit vaccine were seropositive. On days 21 and 28, the ratios of serologically positive to vaccinated pigs were 9/10 and 10/10, respectively. Four of the ten pigs that were vaccinated with the C strain vaccine were positive on day 21 and 9/10 on day 28. However, the results of the NPLA showed that only 4/10 pigs had an antibody titre >1:32 at the end of the trial in both the vaccinated groups, even though the subunit vaccine initiated an earlier and higher level of neutralizing antibodies than the vaccine produced from the C strain. Challenge was performed 28 days after vaccination on four randomly selected pigs from both vaccinated groups. The pigs survived the challenge without showing any clinical signs of classical swine fever (CSF), while two nonvaccinated control pigs died on the 10th and 12th days after infection. 相似文献
88.
R. Bernaś A. Burzyński P. Dębowski A. Poćwierz‐Kotus R. Wenne 《Fisheries Management and Ecology》2014,21(5):398-409
Sea trout, Salmo trutta L., populations in the Slupia River basin have been affected by mass stocking with smolts and fry. This work is focused on a small‐scale differentiation in sea trout populations from one basin with a strong emphasis on the relationship between wild and hatchery origin fish. A total of 216 sea trout were genotyped at 10 microsatellite loci. Global FST obtained by amova was low at 0.0165. Pairwise FST were significant for all tests except wild and stocked adults. The highest pairwise difference was found between the hatchery sample and Kwacza (FST = 0.038). Analysis of the genetic structure revealed micro‐geographical differentiation with four subpopulations. The quality of the artificial spawning was found not to be adequate with a high risk of adverse effects to the whole population. All future stocking actions in the basin should consider the existing population structures. 相似文献
89.
Nikolić A Dimitrijević S Katić-Radivojević S Klun I Bobrć B Djurković-Djaković O 《Acta veterinaria Hungarica》2008,56(3):335-340
To identify areas of risk for canine-related zoonoses in Serbia, the aim of this study was to provide baseline knowledge about intestinal parasites in 151 dogs (65 household pets, 75 stray and 11 military working dogs) from Belgrade. The following parasites, with their respective prevalences, were detected: Giardia duodenalis (14.6%), Ancylostomatidae (24.5%), Toxocara canis (30.5%), Trichuris vulpis (47.0%) and Taenia-type helminths (6.6%). Of all examined dogs, 75.5% (114/151) were found to harbour at least one parasite species. Of these, mixed infections with up to four species per dog occurred in 44.7% (51/114). Infections with all detected species were significantly higher (p < 0.05) in military working (100%) and stray dogs (93.3%) versus household pets (50.8%). Among all parasites, agents with zoonotic potential including Giardia, Ancylostomatidae and Toxocara were detected in 58.3% (88/151) of all examined dogs with a significant difference (p < 0.05) among the subgroups (100%, 62.7% and 46.2% for military working dogs, stray dogs and household pets, respectively). The high prevalence of zoonotic parasites registered in the dog population from a highly urban area in south-eastern Europe indicates a potential risk to human health. Thus, veterinarians should play an important role in helping to prevent or minimise zoonotic transmission. 相似文献
90.
Petrujkić B Samanc H Adamović M Stojić V Petrujkić T Grdović S Sefer D Marković R 《The Japanese journal of veterinary research》2010,58(3-4):171-177
This trial was designed in order to evaluate the incidence of subacute rumen acidosis (SARA) during early lactation and to investigate the possibilities for its prevention by use of a buffering mineral mixture. On the beginning of the trial it was found that the pH value of rumen fluid in 4 animals was lower than normal (pH < 6.0) and that 20% of animals have had SARA. The control and the experimental group of cows were fed the same meal with exception of concentrated feed which in the experimental group contained the mineral mix with buffering activity in amount of 1%. Continuous addition of buffering mineral mixture in the amount of 1% in concentrated feed for early lactation cows successfully prevents SARA formation and leads to increased milk production, as well as increased milk fat and protein content. 相似文献