Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised intercaruncular areas （IC）. In order to better understand aglandular areas called caruncular （C）, and intensely glandular the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins （DEPs） （P 〈 0.05）, functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci-infected domestic poultry in Zhejiang Province,China

A cluster of Chlamydia psittaci (C. psittaci) cases was reported in Zhejiang Province, China, 2019. This study evaluates the extent of the outbreak and determines the source of infection. Real-time PCR and sequencing of the ompA gene of C. psittaci were performed to identify the cases, the domesticated poultry and close contacts. The index patient was a 76-year-old woman with chronic vertigo, and Case 2 was a 64-year-old female farmer with herpes zoster. Both women bought psittaci-infected chickens or ducks from the same mobile street vendor and raised them for 10 days and 23 days before fever onset. There were no direct contact between the two women. C. psittaci test was positive for the two patients, one sick chicken, three healthy ducks and the vendor's chicken cage. Phylogenetic analysis showed that all seven C. psittaci positive samples carried identical ompA genotype A of C. psittaci. Of all of the patients' 148 close contacts, none tested positive for C. psittaci, or developed acute respiratory symptoms. Both patients were discharged after a 4-week hospital stay. In conclusion, the source of this cluster was the poultry infected with C. psittaci, which occasionally cause infections in farmers, but inter-human transmission seems unlikely.     

Spatiotemporal heterogeneity of PPARγ expression in porcine uteroplacenta for regulating of placental angiogenesis through VEGF-mediated signalling

Non-infectious prenatal mortality severely affects the porcine industry, with pathological placentation as a likely key reason. Previous studies have demonstrated that peroxisome proliferator-activated receptor gamma (PPARγ) deficiency causes defects in the uteroplacental vasculature and induces embryonic losses in mice. However, its role in porcine placental angiogenesis remains unclear. In the present study, PPARγ expression was investigated in porcine uteroplacental tissues at gestational day (GD) 25, GD40 and GD70 via quantitative polymerase chain reaction (qPCR), Western blot and immunohistochemistry (IHC). Moreover, the roles of PPARγ in porcine placental angiogenesis were investigated using a cell model of porcine umbilical vein endothelial cells (PUVECs) to conduct proliferation, migration and tube formation assays in vitro and a mouse xenograft model to assess capillary formation in vivo. The results showed that PPARγ was mainly located in the glandular epithelium, trophoblast, amniotic chorion epithelium and vascular endothelium, as indicated by the higher expression levels at GD25 and GD40 than at GD70 in endometrium and by higher expression levels at GD40 and GD70 than at GD25 in placenta. Moreover, PPARγ expression was significantly downregulated in placenta with dead foetus. In PUVECs, knocking out PPARγ significantly inhibited proliferation, migration and tube formation in vitro and inhibited capillary formation in mouse xenografts in vivo by blocking S-phase, promoting apoptosis and downregulating the angiogenic factors of VEGF and its receptors. Overall, the spatiotemporal heterogeneity of PPARγ expression in porcine uteroplacental tissue suggests its vital role in endometrial remodelling and placental angiogenesis, and PPARγ regulates placental angiogenesis through VEGF-mediated signalling.     

影响畜产品安全性的因素及其对策

安全畜产品,概括地说也就是“无疫病、无残留、无污染”的畜产品,在符合无公害畜产品产地环境评价要求的前提下,有毒、有害物质含量在国家法律、法规及有关强制标准规定的安全允许范围内。从养殖到餐桌实行全程质量监控,做到无重大动物疫病、药物残留不超标、对人类生活环境不造成影响。1影响畜产品安全性的因素分析1.1动物的饲养管理方面我国动物的饲养,大多数为农民分散饲养,规模化、集约化程度低,即使是肉类食品出口企业,也多采用“公司 农户”的养殖方式。虽然公司为确保产品质量,对农户实行统一管理,如统一供种、统一采购饲料、统一防…     

实验动物质量检测要求及方法概述

简述了有关实验动物质量检测方法的种类,重点介绍了寄生虫和微生物(细菌、病毒、真菌)检测的操作方法。     

兽药预混剂产品的均一性浅析

产品质量是企业的生命,是企业赖以生存和发展的基础。在国际上,兽药质量概念的内涵已不局限于“检验合格”,即已从“符合性质量”,转化为“适用性质量”。适用性质量的含义为“不使消费者承担安全、质量和疗效的风险”。兽药应是安全高效的,首先应把安全放在第一位。而药品的安全性和药效与其均一性密切相关。由于兽药剂型及给药方式的多样性,对兽药均一性要求比人药更高。预混剂产品的均一性是确保产品质量的重要因素,在预混剂生产过程中,总混工序直接影响产品的均一性,因此,对预混剂总混过程进行分析,对预混剂生产工艺过程中可能影响产品均一性的各种因素进行探讨是非常必要的。     

孕牛血清中早孕因子的分离纯化

采用DEAE－Sepharose(DEAE-琼脂糖凝胶）、CM－Sepharose(CM-琼脂糖凝胶）等离子交换层析、抗孕牛全血清IgG-Sepharose 4B亲和层析等技术，分离纯化妊娠4－5个月健康孕牛全血清中的早孕因子（EPF），用玫瑰花环抑制实验对各阶段产物EPF活性进行检测。结果表明，经层析过柱后的CM－ⅡA提取物有EPF活性。经SDS－PAGE银染显色证实它含有分子量为21、67、80KD的蛋白，实验结果支持21KD的多肽为主要EPF活性物成分。同时实验表明了亲和层析对进一步纯化EPF是可行的。     

<Emphasis Type="Italic">Echinococcus granulosus</Emphasis>: Immunoprotection accompanyied by humoral and cytokine response against secondary hydatidosis in mice immunized with r<Emphasis Type="Italic">Eg.</Emphasis>myophilin

Objects:  

This study aimed to investigate the immunoprotection of the recombinant Eg.myophilin (rEg.myophilin) and tentatively analyze mechanism of this protection.     

High‐level exogenous trans10, cis12 conjugated linoleic acid plays an anti‐lipogenesis role in bovine mammary epithelial cells

Primary bovine mammary epithelial cells (BMECs) were treated by 0, 37.5, 75, 112.5, 150 μmol/L trans10, cis12 conjugated linoleic acid (CLA) to evaluate the effects of different level trans10, cis12 CLA on lipogenesis in BMEC. Addition of 75–150 μmol/L trans10, cis12 CLA reduced significantly the triacylglycerol (TAG) content (P < 0.05), but did not have inhibiting action on cell proliferation (P > 0.05). Treatment with 150 μmol/L trans10, cis12 CLA for 48 h resulted in a 17.1% reduction (P < 0.0001) of medium chain fatty acids (MCFA, C14 < C < C16), a 26.5% reduction (P < 0.0001) of unsaturated fatty acids (UFA) and a corresponding reduction of the mRNA abundance of acetyl coenzyme A (acetylCoA) carboxylase (ACC) (P = 0.046), fatty acid synthase (FAS) (P = 0.017) and stearoylCoA desaturase1 (SCD1) (P = 0.002). Another finding was that trans10, cis12 CLA elevated expression of diacylglycerol acyltransferase2 (DGAT2) (P = 0.020) and long chain acylCoA synthetases (ACSL) (P = 0.032). In conclusion, higher trans10, cis12 CLA, not low trans10, cis12 CLA, inhibited milk fat synthesis and changed fatty acid composition by regulating the expression of FAS, ACC, SCD1, DGAT2 and ACSL.     

禽流感(H9)疫苗免疫间隔期探讨

通过在不同日龄对海兰褐蛋鸡进行禽流感(H9)灭活疫苗的免疫,探讨禽流感灭活疫苗的免疫间隔期。结果表明,开产以后不管防疫2次、3次、4次禽流感灭活疫苗,抗体水平均在6log2以上,且鸡的日龄越大,免疫次数越少,免疫抗体持续期越长。因此,建议从育成到鸡群淘汰的饲养全程中,四次时机恰当、搭配合理的禽流感(H9)疫苗免疫基本上能使得95%以上产蛋期抗体维持于7log2以上。