Lentil Response to Nitrogen Application and Rhizobia Inoculation

Lentils (Lens culinaris L.) are an important component of the dryland farming systems in the western USA. Optimum nitrogen (N) management can enhance yield and quality of lentils. We conducted a field (at two locations, one with previous history of lentil and the other one without lentil history) and a greenhouse study to evaluate response of lentil to the application of rhizobium inoculant and starter N (control, 22 kg N ha^?1 in the form of urea [U], 22 kg N ha^?1 in the form of slow-release or environmentally safe nitrogen [ESN], and 22 kg N ha^?1 U + 22 kg N ha^?1 ESN). In both, the field and the laboratory studies, lentil yield did not respond positively to the experimental treatments. Lentil average yield was 1216 and 1420 kg ha^?1 at the field condition. In this rain-fed system, lentil yield was mainly limited by moisture availability, and the application of an external N did not contribute to the yield enhancement. Both of these treatments, however, increased protein content. Compared to the control, the application of rhizobium plus U and ESN enhanced protein content by about 34% (from 23.1 to 30.9%). The application of U+ESN also considerably increased postharvest residual nitrate (NO₃)-N in the soil, which can be easily leached and creates environmental pollution. Briefly, the application of U+ESN increases lentil protein content, but more efforts are needed to optimize N management in lentils in order to reduce the environmental concerns in the shallow soil.     

Climate change and agriculture: Impacts and adaptive responses in Iran

The impacts of climate change on agriculture are still shadowed with uncertainty. However, climate change is expected to adversely affect Iran's agricultural practices through changes in precipitation, temperature and carbon dioxide fertilization. Therefore, adaptation of this sector to the increasing weather events is imperative. This study is aimed to document the likely impacts of climate change on Iran's agriculture and the current adaptation efforts made by government and farmers. The review of literature shows that changes in rainfall and water endowments will have significant impacts on crop yield, crops' water requirements and income and welfare of farm families. The extent of the changes in yield depends on the crop type, assumptions related to the CO₂ fertilization effect, climate scenarios and adaptation abilities. On adaptation, the government's efforts have been distinguished in the improving agricultural productivity and irrigation development based on current technology, developing new technologies and policy reforms. Farmers' adaptive responses have also been identified. Some conclusions and recommendations are offered to increase the adaptive capacity of farmers and reduce negative impacts of climate change.     

Clinical evaluation of intranasal benzodiazepines, alpha-agonists and their antagonists in canaries

OBJECTIVE: To evaluate the effects of intranasal benzodiazepines (midazolam and diazepam), alpha(2)-agonists (xylazine and detomidine) and their antagonists (flumazenil and yohimbine) in canaries. STUDY DESIGN: Prospective randomized study. ANIMALS: Twenty-six healthy adult domesticated canaries of both sexes, weighing 18.3 +/- 1.0 g. METHODS: In Study 1 an attempt was made to determine the dose of each drug that allowed treated canaries to be laid in dorsal recumbency for at least 5 minutes, i.e. its effective dose. This involved the evaluation of various doses, during which equal volumes of the tested drug were administered slowly into each nostril. In study 2 the onset of action, duration and quality of sedation induced by each drug at its effective dose were evaluated. The efficacy of flumazenil and yohimbine in antagonizing the effects of the sedative drugs was also studied. RESULTS: In study 1 administration of 25 microL per nostril diazepam (5 mg mL(-1) solution) or midazolam (5 mg mL(-1) solution) to each bird caused adequate sedation within 1-2 minutes; birds did not move when placed in dorsal recumbency. After administration of 12 microL per nostril of either xylazine (20 mg mL(-1)) or detomidine (10 mg mL(-1)), birds seemed heavily sedated and assumed sternal recumbency but could not be placed in dorsal recumbency. Higher doses of xylazine (0.5 mg per nostril) or detomidine (0.25 mg per nostril) prolonged sedation but did not produce dorsal recumbency. In study 2 in all treatment groups, onset of action was rapid. Duration of dorsal recumbency was significantly longer (p < 0.05) with diazepam (38.4 +/- 10.5 minutes) than midazolam (17.1 +/- 2.2 minutes). Intranasal flumazenil (2.5 microg per nostril) significantly reduced recumbency time. Duration of sedation was longer with alpha(2)-agonists compared with benzodiazepines. Detomidine had the longest duration of effect (257.5 +/- 1.5 minutes) and midazolam the shortest (36.9 +/- 2.4 minutes). Nasally administered flumazenil significantly reduced the duration of sedation with diazepam and midazolam while yohimbine (120 microg per nostril) effectively antagonized the effects of xylazine and detomidine. CONCLUSION: Intranasal benzodiazepines produce rapid and effective sedation in canaries. Intranasal alpha(2) agonists produce sedation but not sustained recumbency. Specific antagonists are also effective when used by this route. Clinical relevance Intranasal sedative drug administration is an acceptable alternative method of drug delivery in canaries.     

Vulnerability and Adaptation of Livestock Producers to Climate Variability and Change

Rangeland-based animal husbandry has been frequently exposed to the vagaries of weather. While the current rates of climate variability are unprecedented in arid and semiarid regions, climate change is expected to put further pressure on rangelands with medium- and poor-quality forages and increase the vulnerability of households who mainly depend on livestock production. Therefore, it is imperative to ensure that livestock producers increase their resilience to climate variability or change. However, few field-based studies have focused on simultaneous investigation of both vulnerability and adaptation of livestock keepers to climate-related risks. To fill this gap, a field-based research study was conducted in southwest Iran. A survey of 274 herder families, selected using a cluster sampling technique, revealed low, medium and high levels of vulnerability, which were principally distinguished by various degrees of exposure, sensitivity, and adaptive capacity. Further, this study identified the main adaptation strategies employed by the herders of this region (i.e., purchasing fodder; rotational grazing; raising a mixed-herd, on-farm occupation; and postharvest grazing). Results indicated that most herder families applied traditional adaptation strategies in response to climate variability. Findings revealed that herders had to reduce their livestock due to low adaptability. Loan term, purchasing insurance, level of exposure, income, experience, response efficacy, and knowledge were the major determinants of the herders’ adaptation decisions. To increase the resilience of livestock producers against climate change, restructuring traditional livestock production systems, producing participatory knowledge and information for sustainable management of rangelands, and designing or redesigning effective adaptation strategies are required.     

The Role of Uniconazole in Improving Physiological and Biochemical Attributes of Bean (Phaseolus vulgaris L.) Subjected to Drought Stress

Journal of Crop Science and Biotechnology - Adverse environmental conditions such as drought are among the major factors limiting the growth and productivity of land plants. Plant growth regulators...     

Interactive Effect of Fluorescent Pseudomonads Rhizobacteria and Zn on the Growth,Chemical Composition,and Water Relations of Pistachio (Pistacia vera L.) Seedlings under NaCl Stress

The role of fluorescent pseudomonads isolates (pf₀, pf₁, pf₂, and pf₃) and zinc (Zn) (0 and 5 mg kg^?1 soil) interactions in alleviating salinity in pistachio seedlings were investigated. The experiment was conducted following completely randomized design with three replicates. The results revealed that salinity decreased the growth parameters, while application of PGPR (plant growth-promoting rhizobacteria) and Zn increased these parameters. Application of PGPR significantly enhanced phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), Zn, Ca/sodium (Na), and K/Na contents, while decreased the accumulation of Na and chloride (Cl). Application of PGPR and Zn increased the phenolic compounds, sucrose, membrane stability index (MSI), and relative water content (RWC) but reduced the malondialdehyde (MDA) amount. A positive correlation was observed between Zn concentrations with seedling dry weights, phenolic compounds, MSI, and RWC contents. These results indicated that the combined application of PGPR and Zn could be a simple treatment for growth and establishment of pistachio seedlings under Zn deficiency and soil salinity.     

Evaluation of Anticancer and Cytotoxic Effects of Genistein on PC3 Prostate Cell Line under Three-Dimensional Culture Medium

Background:Prostate cancer is a major cause of disease and mortality among men. GNT is an isoflavone found naturally in legumes. Isoflavones, a subset of phytoestrogens, are structurally similar to mammalian estrogens. This study aimed to evaluate the anticancer and cytotoxic effects of GNT on PC3 cell line under 3D culture medium. Methods:The 3D culture was created by encapsulating the PC3 cells in alginate hydrogel. MTT assay, neutral red uptake, comet assay, and cytochrome C assay were used to study the anticancer and cytotoxic effects of GNT at 120, 240, and 480 μM concentrations. Also, NO, catalase, and GSH levels were determined to evaluate the effect of GNT on the cellular stress. The culture medium was used as the negative control.Results:GNT reduced the production of cellular NO and increased the production of catalase and glutathione, confirming the results of the NO test. Evaluation of the toxicity effect of GNT at the concentrations of 120, 240, and 480 μM using comet assay showed that this chemical agent induces apoptosis in PC3 cells in a dose-dependent manner. As the level of cytochrome C in PC3 cells treated with different concentrations of GNT was not significantly different from that of the control, GNT could induce apoptosis in PC3 cells through the non-mitochondrial pathway. Conclusion:The findings of this study disclose that the anticancer effect of GNT on PC3 cells under 3D culture conditions could increase the effectiveness of treatment. Also, the cell survival rate is dependent on GNT concentration. Key Words: Apoptosis, Genistein, Three-dimensional cell culture