Pathotypes of <Emphasis Type="Italic">Plasmodiophora brassicae</Emphasis> causing damage to oilseed rape in the Czech Republic and Poland

Winter oilseed rape (Brassica napus) is an important crop in the Czech Republic and Poland. Clubroot disease caused by the pathogen Plasmodiophora brassicae is a serious and still-growing problem for oilseed rape growers in both countries. The aim of this study was to evaluate the pathotype composition of P. brassicae populations from the Czech Republic and Poland, according to the three evaluation systems, and to determine soil inoculum loads for representative fields via traditional end-point PCR as well as quantitative PCR analysis. There were considerable differences between the populations of P. brassicae from both countries, and the number of pathotypes varied depending on the evaluation system and the threshold used to distinguish susceptible vs. resistant plant reactions. This is the first study comparing the effect of different thresholds. Using an index of disease (ID) of 25 % to distinguish susceptible vs. resistant reactions, there was a total of seven pathotypes identified based on the differentials of Williams, five with the system of Somé et al., and 18 with the European Clubroot Differential (ECD) set. However, based on a threshold of 50 %, there were nine pathotypes according to the evaluation system by Williams, four based on the differentials of Somé et al., and 15 with the ECD set. Changing of the thresholds led to the reclassification of some pathotypes. Several pathotypes were common in both countries. High amounts of pathogen DNA were found in many of the field soils analysed by quantitative PCR. There was a weak correlation between soil pH and infestation of P. brassicae for the Polish soils.     

Population genetic structure of Mycosphaerella graminicola and Quinone Outside Inhibitor (QoI) resistance in the Czech Republic

Damage caused by the wheat pathogen Mycosphaerella graminicola increased rapidly during the last two decades in the Czech Republic. We collected isolates from naturally infected fields in seven wheat-growing locations and analysed these using eight microsatellite markers. All markers were highly polymorphic. We found a high degree of genetic diversity and low clonality within all sampled Czech populations. We identified 158 unique multilocus haplotypes among 184 isolates. Field populations showed weak genetic structure but we detected more differentiation between climatic regions within the Czech Republic. We compared the Czech field populations to populations from the United Kingdom, Germany and Switzerland and found a marked differentiation between Czech populations and Western European populations. We hypothesize that decades of different agricultural practices, including the use of different wheat cultivars, may explain this genetic differentiation. We detected a rapid increase in QoI fungicide resistance during the sampling period from 2005 to 2011, coinciding with the widespread application of this class of fungicides in the Czech Republic. M. graminicola populations in the Czech Republic underwent a rapid adaptive evolution from sensitivity to resistance similar to what was described earlier in Western Europe.     

Landscape composition is the major driver of the taxonomic and functional diversity of tropical frugivorous birds

Landscape Ecology - Anthropogenic land use and cover changes impact biodiversity worldwide. However, ecological groups are differently affected by landscape composition and configuration....     

The importance of landscape context for songbirds on migration: body mass gain is related to habitat cover

Landscape context influences many aspects of songbird ecology during the breeding season. The importance of landscape context at stopover sites for migrating songbirds, however, has received less attention. In particular, landscape context may affect the availability and quality of food for refueling during stopovers, which is critical for successful migration. We evaluated the influence of woody habitat cover in the surroundings of stopover sites at several spatial extents on the hourly changes of body mass in two species of European-African forest-dwelling songbird migrants (Willow Warbler, Phylloscopus trochilus, and the Eurasian Redstart, Phoenicurus phoenicurus). Data were sampled by standardized methods from a network of ringing stations throughout Europe during the falls of 1994–1996. In both species, hourly body mass gain calculated for first captures increased with woody habitat cover. We found a similar logarithmic relationship for both species, although for Willow Warblers mass gain was more strongly related to the habitat cover within 5 km, in contrast to 3 km for Redstarts. For Willow Warblers, where sufficient data are available for each year, the relationship is consistent over the years. The shape of the relationship suggests existence of a threshold of landscape suitability for refueling at stopover sites: in sites with less than 10% of woody habitat cover, birds tend to lose body mass or to gain mass at a lower rate. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Relative effects of management and environmental conditions on performance and survival of populations of a terrestrial orchid, Dactylorhiza majalis

For population viability analysis of endangered orchid populations, it is crucial to disentangle the effects of weather and management from intrinsic orchid dynamics. When doing this, typically several months’ average temperatures and/or sums of precipitations are compared with some characteristics of plant performance. Here we tested, whether short averaging intervals (1-2 weeks) are more closely correlated with orchid performance. We used 5 years of data from five Dactylorhiza majalis populations, and have shown that the improvement of prediction by shortening the interval over which the temperatures are averaged or precipitation summed, even if detectable, is only weak and not significant. This, however, may be due to low weather variability during the study. Regarding the second aspect, the effect of management (presence or absence of mowing), we have found that leaf area of D. majalis at the regularly mown site was larger than that at the sites which were mown only once in 2 years, but we did not detect a significant effect of the absence of mowing on the incidence of flowering. Mowing can affect orchid performance in two ways: by reduced shading of orchids and by reducing competition with other species. Therefore, we have determined the co-occurring species associated with presence or absence of mowing and found that shading significantly affected the length of the flower stalk, the ratio of leaf width to leaf length at the end of the season, but did not affect seed weight and probability of flowering the next year. We conclude that the most appropriate management for D. majalis is mowing at least once a year, ideally at the end of June/beginning of July, after its fruiting.     

Genetic structure of the Amur tiger (Panthera tigris altaica) population: Are tigers in Sikhote‐Alin and southwest Primorye truly isolated?

We used molecular genetic analyses to noninvasively identify individual Amur tigers and define subpopulations of tigers in the Russian Far East. We identified 63 individuals after genotyping 256 feces, 7 hair and 11 blood samples collected within southern, central and northern Sikhote‐Alin, as well as Southwest Primorye. Analysis of nuclear DNA at 9 microsatellite loci demonstrated greater genetic similarity between animals from southern and northern Sikhote‐Alin (some 500 km apart) than between animals from Ussuriskii State Nature Reserve and Southwest Primorye (less than 10 km apart at their nearest point), suggesting that a true barrier exists preventing movements of tigers between Southwest Primorye and the southern Sikhote‐Alin Mountains.     

Adaptation of wheat rusts to the wheat cultivars in former Czechoslovakia

Summary In former Czechoslovakia virulence of rusts attacking wheat was studied since the sixties. Since the same time genes for resistance in the registered cultivars were identified. The role of Berberis and Thalictrum as alternate hosts for stem rust and leaf rust, respectively, was investigated as well. Determined changes of virulence in rust populations could only partially be ascribed to changes of resistance genes in the grown cultivars. Unnecessary genes for virulence had no negative effect on the fitness of the pathogen. All tested samples of aeciospores from barberries attacked rye, not wheat. None of Thalictrum species occurring in the Czech and Slovak Republics was found to host wheat leaf rust. However, the sexual stage of wheat stem rust and wheat leaf rust could be induced on Berberis vulgaris and Thalictrum speciosissimum, respectively. General epidemiological conclusions are drawn from the results and experience of the last 35 years.     

Disease resistance in the genusAegilops L. — stem rust,leaf rust,stripe rust,and powdery mildew

Summary Resistance of the GaterslebenAegilops collection was studied in relation to the following wheat diseases: stem rust(Puccinia graminis), leaf rust(Puccinia recondita), stripe rust(Puccinia striiformis) and powdery mildew(Erysiphe graminis). Numerous sources of complete, combined or single resistance have been detected in 487 accessions of 21Aegilops species. Potential donors of the complete resistance have been found in diploidAe. speltoides, Ae. longissima ssp.sharonensis, Ae. mark-grafii and tetraploidAe. geniculata. Methods of a transfer of resistance genes from particularAegilops species to the common wheat,Triticum aestivum, are discussed.

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Krankheitsresistenz in der GattungAegilops L. — Schwarzrost, Braunrost, Gelbrost und Mehltau

Zusammenfassung Die GaterslebenerAegilops-Kollektion wurde auf Resistenz gegen folgende Weizen-Krankheiten untersucht: Schwarzrost(Puccinia graminis), Braunrost(Puccinia recondita), Gelbrost(Puccinia striiformis) und Mehltau(Erysiphe graminis). Zahlreiche Quellen für Resistenz gegenüber einzelnen Krankheiten, kombinierte Resistenz und auch vollständige Resistenz gegenüber allen Erregern konnten unter den 487 Sortimentsnummern aus 21Aegilops-Arten gefunden werden. Potentielle Donoren mit vollständiger Resistenz wurden bei den diploiden SippenAe. speltoides, Ae. longissima ssp.sharonensis undAe. markgrafii sowie bei der tetraploidenAe. geniculata nachgewiesen. Methoden zur Übertragung der Resistenzgene von den einzelnenAegilops-Arten auf den Weizen,Triticum aestivum, werden diskutiert.

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Aegilops L. —

Aegilops :Puccinia graminis, P. recondita, P. striiformis, Erysiphe graminis. , , 487 21 Aegilops. , Ae. speltoides, Ae. longissima ssp.sharonensis, Ae. markgrafii Ae. geniculata. Aegilops Triticum aestivum.

     

Late Mousterian persistence near the Arctic Circle

Palaeolithic sites in Russian high latitudes have been considered as Upper Palaeolithic and thus representing an Arctic expansion of modern humans. Here we show that at Byzovaya, in the western foothills of the Polar Urals, the technological structure of the lithic assemblage makes it directly comparable with Mousterian Middle Palaeolithic industries that so far have been exclusively attributed to the Neandertal populations in Europe. Radiocarbon and optical-stimulated luminescence dates on bones and sand grains indicate that the site was occupied during a short period around 28,500 carbon-14 years before the present (about 31,000 to 34,000 calendar years ago), at the time when only Upper Palaeolithic cultures occupied lower latitudes of Eurasia. Byzovaya may thus represent a late northern refuge for Neandertals, about 1000 km north of earlier known Mousterian sites.