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61.
Background
Invasive Australian populations of redlegged earth mite, Halotydeus destructor (Tucker), are evolving increasing organophosphate resistance. In addition to the canonical ace gene, the target gene of organophosphates, the H. destructor genome contains many radiated ace-like genes that vary in copy number and amino acid sequence. In this work, we characterise copy number and target-site mutation variation at the canonical ace and ace-like genes and test for potential associations with organophosphate insensitivity. This was achieved through comparisons of whole-genome pool-seq data from alive and dead mites following organophosphate exposure.Results
A combination of increased copy number and target-site mutations at the canonical ace was associated with organophosphate insensitivity in H. destructor. Resistant populations were segregating for G119S, A201S, F331Y at the canonical ace. A subset of populations also had copy numbers of canonical ace > 2, which potentially helps overexpress proteins carrying these target-site mutations. Haplotypes possessing different copy numbers and target-site mutations of the canonical ace gene may be under selection across H. destructor populations. We also detected some evidence that increases in copy number of radiated ace-like genes are associated with organophosphate insensitivity, which might suggest potential roles in sequestration or breakdown of organophosphates.Conclusion
Different combinations of target-site mutations and (or) copy number variation in the canonical ace and ace-like genes may provide non-convergent ways for H. destructor to respond to organophosphate selection. However, these changes may only play a partial role in organophosphate insensitivity, which appears to have a polygenic architecture. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献62.
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64.
Stephanie Scheemaeker Eva Vandermeulen Richard Ducatelle Lisa Stammeleer Nausikaa Devriendt Tom Roggeman Sylvie Daminet 《Veterinary and comparative oncology》2023,21(2):349-356
Currently, a histological diagnosis of highly vascularized canine (c) thyroid carcinoma (TC) is primarily obtained following excisional biopsy (EB) through thyroidectomy. Non-EBs are contraindicated in unresectable invasive cTCs due to their highly vascularized nature, which subsequently, lack histological diagnosis. We hypothesised ultrasound-guided core needle biopsy (UGCNB) to be a safe biopsy technique to obtain an accurate histological diagnosis in unresectable TCs. Nine client-owned dogs with suspected naturally occurring TC, presented for surgical excision, were included. First, a UGCNB was taken from the cervical tumour, followed by EB. Haemorrhage following UGCNB was evaluated preoperatively and once the tumour was surgically exposed by visual inspection and ultrasonography. Histological analysis, including cell organisation, tumour capsular and vascular invasion, and immunohistochemistry were performed and compared between both biopsy specimens (i.e., UGCNB and EB) of the same dog. Pre- and peroperative visual inspection revealed minor, localised haemorrhage, subsequent to the UGCNB, in 7/9 dogs. Histology of the EBs confirmed TC in 8/9 dogs and was inconclusive in 1/9 dogs. Histology of the UGCNBs revealed neoplastic thyroid tissue in 7/9 UGCNBs and was inconclusive in 1/9 UGCNBs. The remaining UGCNB contained no mass related tissue and was, therefore, excluded. Histological parameters (i.e., cell organisation, tumour capsular and vascular invasion) were not concordant between 6/8 included UGCNBs and their respective EB. Immunolabelling for thyroglobulin and calcitonin was concordant between all eight included UGCNBs and their respective EB. The remaining evaluated immunohistochemical markers (i.e., cyclooxygenase-2 [COX-2], P-glycoprotein and vascular endothelial growth factor [VEGF]) were concordant between the included UGCNBs and the EBs in 6/8 dogs. To conclude, UGCNBs can be safely obtained in suspected cTCs and enable a reliable diagnosis of the thyroid origin, thyroid cell origin and potential therapeutic markers such as COX-2, P-glycoprotein and VEGF. Subsequently, UGCNB enables clinicians to establish an individually tailored treatment plan in dogs with unresectable TC. 相似文献
65.
R. L. McCown B. H. Wall P. S. Carberry J. N. G. Hargreaves K. L. Green P. L. Poulton 《Irrigation Science》1990,11(3):189-195
Summary This paper reports on the design and performance of an irrigation system associated with a rain shelter. The shelter is used in the closed position as a platform for multiple spray lines. This system automatically supplies, at a specified time, water of specified depth and delivery rate, either uniformly or on a specified gradient across the sheltered area. Variations in crop water deficits and yields have conformed closely to imposed water gradients. 相似文献
66.
The standardized ASCE Penman–Monteith and FAO-56 equations were used to estimate reference evapotranspiration (ET0) using estimated and measured net radiation (Rn) and soil heat flux (G), based on hourly and daily meteorological data. The estimates were evaluated against lysimeter measurements. The results indicate that using measured or estimated values of Rn and G can have significant effect on the accuracy of the ET0 estimations, especially when calculations were made on an hourly basis. The FAO-56 version performed very well during the irrigation season on a daily basis. The use of measured Rn and G did not improve ET0 estimation on a daily basis, therefore, the use of estimated Rn and G appears to be dependable when calculations are based on 24-h weather data. When daily ET0 was calculated from hourly estimations, the results were different depending on the version used. The ASCE version was more accurate, especially when Rn and G were measured. Therefore, measurement of Rn and G may have potential to improve estimation only when daily ET0 is calculated from hourly estimations. The PM FAO-56 version was always a little less accurate than the ASCE version. For hourly calculations, using a constant surface resistance (as in FAO-56 version), the PM method underpredicted for high evaporative demand and vice versa. The ASCE version performed better than PM FAO-56 version when Rn and G were measured and estimated. Therefore, ASCE version tended to provide quite accurate values of hourly ET0, even using estimated values of Rn and G. As conclusion, the methods proposed by FAO-56 for estimating Rn and G tended to produce accurate estimates for daily and hourly ET0 under semiarid conditions and can be used with some degree of confidence for estimating ET0. In addition, results suggest that the ASCE standardized equation on an hourly basis improved the accuracy of ET0 estimation with respect to the FAO-56 version. 相似文献
67.
Moqanaki Ehsan M. Milleret Cyril Tourani Mahdieh Dupont Pierre Bischof Richard 《Landscape Ecology》2021,36(10):2879-2895
Landscape Ecology - Spatial capture-recapture (SCR) models are increasingly popular for analyzing wildlife monitoring data. SCR can account for spatial heterogeneity in detection that arises from... 相似文献
68.
Sachin Vyas Maurizio Bettiga Ulrika Rova Paul Christakopoulos Leonidas Matsakas Alok Patel 《Marine drugs》2022,20(3)
The marine microorganisms thraustochytrids have been explored for their potential in the production of various bioactive compounds, such as DHA, carotenoids, and squalene. Squalene is a secondary metabolite of the triterpenoid class and is known for its importance in various industrial applications. The bioinformatic analysis for squalene synthase (SQS) gene (the first key enzyme in the tri-terpenoid synthesis pathway), that is prevailing among thraustochytrids, is poorly investigated. In-silico studies combining sequence alignments and bioinformatic tools helped in the preliminary characterization of squalene synthases found in Aurantiochytrium limacinum. The sequence contained highly conserved regions for SQS found among different species indicated the enzyme had all the regions for its functionality. The signal peptide sequence and transmembrane regions were absent, indicating an important aspect of the subcellular localization. Secondary and 3-D models generated using appropriate templates demonstrated the similarities with SQS of the other species. The 3-D model also provided important insights into possible active, binding, phosphorylation, and glycosylation sites. 相似文献
69.
Rell L. Parker John Du Richard L. Shinn Adam G. Drury FangChi Hsu John L. Roberston Thomas E. Cecere Avril U. Arendse John H. Rossmeisl 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2022,36(2):694
BackgroundSeizures in the early postoperative period after intracranial surgery may affect outcome in dogs.ObjectivesTo determine the incidence of early postoperative seizures (EPS) in dogs with brain tumors, identify specific risk factors for EPS, and determine if EPS affects outcome.AnimalsEighty‐eight dogs that underwent 125 intracranial surgeries for diagnosis and treatment of rostrotentorial brain tumors.MethodsRetrospective cohort study. All patients with a diagnosis of rostrotentorial brain tumor from 2006 to 2020 were included. Early postoperative seizures were diagnosed by observation of seizure activity within 14 days of neurosurgery. Previously diagnosed structural epilepsy, perioperative anticonvulsant drug (ACD) use, magnetic resonance imaging (MRI), and tumor characteristics were evaluated. Outcome measures included neurologic and nonneurologic complications, duration of hospitalization, and survival to discharge.ResultsDogs with rostrotentorial brain tumors had EPS after 16/125 (12.8%) neurosurgical procedures (95% confidence interval [CI], 7%‐19%). Presence of previous structural epilepsy was not associated with EPS risk (P = 1). Perioperative ACD use also was not associated with EPS (P = .06). Dogs with EPS had longer hospitalization (P < .001), were more likely to have neurologic complications postsurgery (P = .01), and were less likely to survive to discharge (P = .01).Conclusions and Clinical ImportanceIt is difficult to predict which dogs are at risk of EPS because the presence of previous structural epilepsy and the use of perioperative ACDs was not associated with EPS. However, seizures in the early postoperative period are clinically important because affected dogs had prolonged hospitalization, more neurologic complications, and decreased short‐term survival. 相似文献
70.
过去人们常认为,由于刚出壳雏鸡的免疫系统尚未发育成熟,同时肠道内缺乏有益的微生物,因而处于高度的敏感期,此时接种沙门氏菌苗产生的保护作用很有限。然而,最新的研究表明,在这些敏感期接种疫苗,可诱发产生保护作用。 相似文献