A number of the members of the Noctuidae (subfamily Plusiinae) are polyphagous insects that infest a variety of economically important crops world-wide. These hosts include but are not limited to cotton, vegetable and field crops and also such specialty crops as mint. Many of these crops require multiple applications of chemical pesticides to protect them from various loopers and other economic pests. One proposed alternative to the use of chemical pesticides to protect these crops is the development of insect-specific viruses of the family Baculoviridae. Hundreds of these viruses of the genus Nucleopolyhedrovirus have been isolated, many from economically important Lepidoptera. The advent of the discovery that some of these viruses may have a broad host range increased the interest in the development of them as microbial agents throughout the world. The broad host range (over 30 species in some cases) provided the possibility to control not only one species, but a complex of lepidopterous pests infesting specific or multiple crops. From the 1960s to the present, extensive basic and applied research has been conducted on the multi-nucleocapsid (MNPV) forms within which a broad host range appears to be characteristic. Of these, the virus isolated from the alfalfa looper, Autographa californica (Speyer) (AcMNPV) has been the most studied, particularly as related to microbial control. Two of the viruses isolated from loopers have been registered in the USA. Although efficacy has been demonstrated for loopers, there is a paucity of data on the other important species that may simultaneously infest looper hosts. Because of their relatively slow action, the viruses will probably be used in integrated programs with chemical pesticides applied during critical periods of high population pressure. Further research needs to be conducted to establish efficacy for other hosts, improved production methods either in vivo or in vitro, increased field persistence as related to timing and frequency of applications, and finally the utility of genetically engineered MNPVs as microbial pesticides. The potential of these organisms has been demonstrated and new developments are being made to increase the overall efficiency and economics of their use. These improvements can only increase interest in use of the baculoviruses in pest management systems for loopers and other pests. 相似文献
The effects of increasing levels of dietaryphospholipids (PL) on the reproductive performance,egg and larval quality, and lipid composition offemales of the freshwater prawn Macrobrachiumrosenbergii were investigated. Three isolipidic dietscontaining similar amounts of highly unsaturated fattyacids but varying levels of PL (0.8, 2.4 and 4.6%)were fed during 180 days to three groups of eightfemales originating from Thai ponds. No significantdifferences were observed for fecundity, egg size andhatchability, starved larvae size, and size, survivaland tolerance to stress of 8 day-old larvae.Similarly, no major differences in the lipidcomposition of the midgut gland, ovaries and muscletissue of females could be detected. Results indicatethe lack of need of dietary supplementation of PL forM. rosenbergii broodstock, as previouslyreported for earlier life stages. It is suggested thatthe basal level of 0.8% dietary PL was sufficient tomeet the dietary demands of the prawn broodstock. ThePL requirements of M. rosenbergii broodstock, ifany, may be satisfied in commercial feeds through theinclusion of ingredients containing some phospholipidsendogenously. 相似文献
A segregating population of doubled-haploid lines issued from the cross between the wheat (Triticum aestivum L. em. Thell) cultivars Courtot, resistant to several isolates of powdery mildew (Blumeria graminis DC. f. sp. tritici Em. Marchal), and Chinese Spring (susceptible) was used to map Mlar, a gene carried by Courtot and conferring resistance to this pathogen. The assignation of Mlar using monosomic lines of Courtot was confirmed by the mapping analysis. Mlar was located on the short arm of the chromosome 1A, in the vicinity of the locus XGli-A5 coding for storage proteins. This result was in accordance with those demonstrating that Mlar was an allele of the Pm3 locus (Pm3g), a gene also involved in the resistance to powdery mildew.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
Summary Two nonparametric statistical techniques are presented which allow the user to evaluate the effect of a treatment on an n-dimensional
set of variables associated with forest products. The multi-response permutation procedures (MRPP) are a broad category of
permutation techniques based on a variety of distance functions. Earlier work on this subject has demonstrated that when the
distance function is selected appropriately, one-dimensional MRPP represent a rational alternative to traditional comparative
statistical analysis techniques, such as the Student's t-test (t-test). In the work presented herein, MRPP are broadened to
include analyses on the effect of a treatment on data were containing multiple variables which may be correlated. These tests
are a MRPP based on Euclidean distance (MRPP-E) and a MRPP motivated by Hotelling's T2 test (MRPP-H) which can account for the variance-covariance structure of the data. Four hundred eighteen data points representing
the moduli of elasticity (MOE) and rupture (MOR) of eight foot-long (244 cm), nominal 2 × 4 inch2 (51 × 102 mm2), No. 2 grade, Douglas-fir, dimension lumber from seven growing regions in the western United States were selected for use
in this study. Data were analyzed using one- and two-dimensional, classical, parametric techniques (i.e. t-test and Hotelling's
T2 test) and the more intuitive and nonparametric MRPP in similar dimensions. The results indicated that the MOE demonstrated
some degree of sensitivity to the growth region, while the MOR proved to be insensitive. Also, considerable differences in
inference drawn regarding the presence of statistically significant differences between data sets existed as a function of
the analytical test method used. The unique structure of the data encountered in this study showed that MRPP-E was insufficiently
sensitive to the variance-covariance structure of the data. Visual examination of the data suggested that MRPP-H is more appropriate
for the present data than MRPP-E.
Received 7 October 1998 相似文献
There is a lack of knowledge of the arthropod communities in both wild hazelnut and cultivated hybrid hazelnut ecosystems in the Midwestern United States. Our goal was to characterize the composition of these arthropod communities in hazelnut plantings. We surveyed six experimental plantings of hazelnuts in Wisconsin and two in Minnesota during growing seasons, from May 2017 to August 2021. We used four methods to survey the arthropod community of these plantings: ad libitum survey of specimen observations and collection, dissection of hazelnut buds and nuts, cone traps placed on the ground, and beat sheet sampling of plants. We registered 116 different morphospecies of five classes and 83 families. Arthropods with known feeding habits were grouped into four guilds: 59 herbivores, 36 carnivores (predators, parasites, and parasitoids), 4 decomposers (detritivores and scavengers), and 4 omnivores. While we registered 12 herbivorous species that could potentially cause damage to hazelnuts, we directly observed nine of them feeding upon or damaging plant parts: Phytoptus avellanae s.l. Nalepa (Eriophyoidea: Phytoptidae), Curculio obtusus Blanchard and Strophosoma melanogrammum Förster (Coleoptera: Curculionidae), Popillia japonica Newman (Coleoptera: Scarabeidae), Parthenolecanium sp. (Hemiptera: Coccidae), Euschistus servus euschistoides Say (Hemiptera: Pentatomidae), aphids (Hemiptera: Aphididae), Acleris sp., and Choristoneura rosaceana Harris (Lepidoptera: Tortricidae). We also registered eight species of parasites or parasitoids that could offer biocontrol services in the cropping system. These findings provide a foundation for future targeted studies on potential pests and beneficial arthropods, as well as ecological interactions within the hybrid hazelnut ecosystem in the Midwest. 相似文献
The aim of this preliminary study was to evaluate the influence of different feeding regimes on the quality of reproduction in pikeperch. Three diets were tested: forage fish (FF), a commercial dry feed (DD) and a mix of both (FD). The diets were given to fish throughout a complete reproductive cycle. During the spawning season, couples were injected with human chorionic gonadotropin (hCG) and let to spawn on nests. Proportion of running males, spawning and hatching success and larval quality (weight, length, body protein, total lipid, fatty acid and lipid class compositions and resistances to osmotic shock and starvation stress tests at hatching) were evaluated. The proportion of running male was lower in the DD group than in the FF and FD groups (54% for DD against 76–89% for FF and FD). In addition, 25%, 62.5% and 75% of injected couples gave spawning that hatched in DD, FF and FD groups respectively. Larval quality parameters were not significantly different between treatments. The results indicate that overall quality of reproduction was higher in FF and FD treatments than in DD. It suggests that the dry feed used was not totally adequate for pikeperch reproduction. Relations between breeder reproductive performances and the feed compositions are discussed. 相似文献
Five years of field, laboratory, and numerical modelling studies demonstrated ecosystem‐level mechanisms influencing the mortality of juvenile pink salmon and Pacific herring. Both species are prey for other fishes, seabirds, and marine mammals in Prince William Sound. We identified critical time‐space linkages between the juvenile stages of pink salmon and herring rearing in shallow‐water nursery areas and seasonally varying ocean state, the availability of appropriate zooplankton forage, and the kinds and numbers of predators. These relationships defined unique habitat dependencies for juveniles whose survivals were strongly linked to growth rates, energy reserves, and seasonal trophic sheltering from predators. We found that juvenile herring were subject to substantial starvation losses during a winter period of plankton diminishment, and that predation on juvenile pink salmon was closely linked to the availability of alternative prey for fish and bird predators. Our collaborative study further revealed that juvenile pink salmon and age‐0 herring exploit very different portions of the annual production cycle. Juvenile pink salmon targeted the cool‐water, early spring plankton bloom dominated by diatoms and large calanoid copepods, whereas young‐of‐the‐year juvenile herring were dependent on warmer conditions occurring later in the postbloom summer and fall when zooplankton was composed of smaller calanoids and a diversity of other taxa. The synopsis of our studies presented in this volume speaks to contemporary issues facing investigators of fish ecosystems, including juvenile fishes, and offers new insight into problems of bottom‐up and top‐down control. In aggregate, our results point to the importance of seeking mechanistic rather than correlative understandings of complex natural systems. 相似文献
We evaluated the annual process of oocyte and ovarian development of the planktivorous Amazonian catfish, the mapará (Hypophthalmus marginatus), aiming to support captive reproduction for domestication. A total of 149 females were captured from the Tocantins River at the Tucuruí Dam (3°49'55"S, 49°39'9"W) in 13 sampling events. For each individual, we evaluated ovary histology and plasma steroid concentrations. Two annual reproductive cycles, with similar characteristics, including a long period of rest, a short vitellogenic stage and a well‐defined spawning season from November to March, were described. A 17β‐estradiol (E2) rise and a 17α,20β‐dihydroxy‐4‐pregnen‐3‐one (DHP) peak were associated with vitellogenesis and spawning season, respectively, in the first cycle but not in the second reproductive cycle. In conclusion, the studied species presents a reproductive cycle similar to that of other migratory total spawners; however, unusually for this group of fish, there were three (but not two) batches of vitellogenic oocytes in “in maturation” and “mature” stage ovaries, pointing to the possibility of more than one spawn during the spawning season. 相似文献
Chloride cell morphology and density in the gill and opercular membrane of coho salmon (Oncorhynehus kisutch) were examined during the 1982 and 1983 smolt-seasons for structural correlates to smoltification-associated changes in hypoosmoregulatory ability and plasma hormone levels. Although not synchronous in the two years, plasma thyroxine levels displayed two peaks each year. In 1982, changes in gill Na+,K+-ATPase activity were preceded by elevations in plasma thyroxine. In 1983, the increase in enzyme activity corresponded with the April increases in plasma thyroxine and prolactin. Seawater (SW) acclimation experiments showed discrete increases in SW tolerance which coincided with changes in gill enzyme activity. Although these data suggest a relationship between SW tolerance and plasma thyroxine levels, the patterns in the two years were different. A significant peak in plasma prolactin levels in April suggests possible involvement in the parr-smolt transformation.
Chloride cells were studied in the opercular membrane, a chloride cell-containing epithelium lining the branchial side of the operculum, and the gill epithelium. Opercular membrane chloride cells (1982), marked with a mitochondrion-specific fluorescent stain, increased nearly two-fold in late May, concurrent with the second increase in gill Na+,K+-ATPase activity and SW tolerance. Gill morphology was examined (1983) with scanning and transmission electron microscopy and light microscopy. Initially, the gill filament surface appeared rough but became smoother during smoltification and rough again toward the end of smoltification. Two mitochondrion-rich (chloride) cell types were present in the gill epithelium during smoltification. The electron-lucent type I cell contained large, circular mitochondria while the electron-dense type II cell contained thin, elongate mitochondria. Ultrastructurally, many type I cells appeared disrupted which may indicate cellular degeneration or a special response of type I cells to fixation. However, it was unusual to find either intact or disrupted type I cells after March. Chloride cell density (type I plus type II) remained constant during smoltification. The inability to distinguish between these cell types with the light microscope may have obscured changes in their individual densities. The data from both the opercular membrane and the gill epithelium and their relationship with gill Na+,K+-ATPase activity suggest that the development of SW tolerance during smoltification is partially due to changes in chloride cell enzyme activity and density. The presence of two mitochondrion-rich cell types is discussed with regard to their possible role in the hypoosmoregulatory changes which occur during smoltification. 相似文献