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排序方式: 共有117条查询结果,搜索用时 15 毫秒
31.
Amara S Abdelmelek H Garrel C Guiraud P Douki T Ravanat JL Favier A Sakly M Ben Rhouma K 《The Journal of reproduction and development》2008,54(2):129-134
The aim of this study was to investigate the antioxidant role of zinc (Zn) in the Cd-exposed testes of Wistar rats. Subchronic exposure to Cd (CdCl(2), 40 mg/l, per os) for 30 days resulted in a significant reduction in growth rate (-11%) and relative weights of testes (-36%) and seminal vesicles (-80%). Treated rats displayed a decrease in testicular and plasma testosterone levels, respectively (-70%, P<0.05; -48%, P<0.05), epididymal sperm count (-22%, P<0.05), and spermatozoa motility (-35%, P<0.05). In contrast, Cd increased the malondialdehyde (+46%, P<0.05), metallothionein (+200%, P<0.05), and 8-oxodGuo concentrations (+71%, P<0.05) in the testis. In the gonad, Cd decreased the GPx (-30%, P<0.05), CAT (-32%, P<0.05), mitochondrial Mn-SOD (-34%, P<0.05), and cytosolic CuZn-SOD (-32%, P<0.05) activities. Zinc supplementation (ZnCl(2), 40 mg/l, per os) in the Cd-exposed rats restored the activities of GPx, CuZn-SOD, and Mn-SOD in the testes to the levels of the control group. Moreover, zinc administration was capable of reducing the elevated levels of malondialdehyde in the testis. Interestingly, zinc supplementation attenuated DNA oxidation induced by Cd in the gonad and restored the testosterone level and sperm count to the levels of the control group. Zinc administration minimized oxidative damage and reversed the impairment of spermatogenesis and testosterone production induced by Cd in the rat testis. 相似文献
32.
Éric Niqueux Jean-Paul Picault Michel Amelot Chantal Allée Josiane Lamandé Carole Guillemoto Isabelle Pierre Pascale Massin Guillaume Blot François-Xavier Briand Nicolas Rose Véronique Jestin 《Veterinary microbiology》2014
EU annual serosurveillance programs show that domestic duck flocks have the highest seroprevalence of H5 antibodies, demonstrating the circulation of notifiable avian influenza virus (AIV) according to OIE, likely low pathogenic (LP). Therefore, transmission characteristics of LPAIV within these flocks can help to understand virus circulation and possible risk of propagation. This study aimed at estimating transmission parameters of four H5 LPAIV (three field strains from French poultry and decoy ducks, and one clonal reverse-genetics strain derived from one of the former), using a SIR model to analyze data from experimental infections in SPF Muscovy ducks. The design was set up to accommodate rearing on wood shavings with a low density of 1.6 ducks/m2: 10 inoculated ducks were housed together with 15 contact-exposed ducks. Infection was monitored by RNA detection on oropharyngeal and cloacal swabs using real-time RT-PCR with a cutoff corresponding to 2–7 EID50. Depending on the strain, the basic reproduction number (R0) varied from 5.5 to 42.7, confirming LPAIV could easily be transmitted to susceptible Muscovy ducks. The lowest R0 estimate was obtained for a H5N3 field strain, due to lower values of transmission rate and duration of infectious period, whereas reverse-genetics derived H5N1 strain had the highest R0. Frequency and intensity of clinical signs were also variable between strains, but apparently not associated with longer infectious periods. Further comparisons of quantitative transmission parameters may help to identify relevant viral genetic markers for early detection of potentially more virulent strains during surveillance of LPAIV. 相似文献
33.
Newcastle disease, induced by a type 1 Avian Paramyxovirus (APMV-1), is one of the most serious poultry diseases. APMV-1 are divided into two classes based on genetic analysis: class II strains have been recovered from wild or domestic birds and include virulent and avirulent isolates whereas class I strains have been mainly isolated from wild birds and are avirulent. Within class I, a new proposed genotype has recently been reported. The only full genome strain of this group is presently characterised from the point of view of codon usage with reference to class I and class II specificities. Class-specific residues were identified on HN and F proteins that are the two major proteins involved in cell attachment and pathogenicity. Comparison of protein patterns and codon usage for this newly identified APMV-1 strain indicates it is similar to class I viruses but contains a few characteristics close to the viruses of class II. Transmission of viruses from this recently identified divergent group from wild birds to domestic birds could have a major impact on the domestic poultry industry. 相似文献
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35.
Linkages between forest dynamics and ecosystem processes are poorly understood and this limits our ability to adequately estimate future changes in forest ecosystems due to human-induced global change. In particular at the single tree level, our understanding of temporal and spatial changes of belowground properties during forest succession is limited. Thus, our aim was to test whether we find a spatial and temporal gradient in nutrient availability and an associated shift in microbial community structure with increasing distance and age of single trees. We found that inorganic nitrogen was less available below the crown of single trees, while soluble organic carbon (DOC) was much more abundant, in particular in the inner zone of influence, i.e. close to the stem. The fungal:bacterial PLFA ratio was greater while microbial biomass carbon (MicC) was lower below the tree crown, indicating a strong influence of trees on spatial patterns of microbial biomass and community structure. Moreover, the positive correlation between MicC and total extractable N, and the negative correlation between fungal:bacterial biomass and δ15N, suggested that the microbial biomass was N limited below the tree crown and as a consequence nutrient cycling was presumably decelerated compared to open conditions. We also found a temporal pattern of increasing surface soil C and N content with increasing tree age (up to 250 years), underlining the significant role of single trees in creating spatial and temporal heterogeneity in forests. 相似文献
36.
Invasive bacteria actively induce their own uptake by phagocytosis in normally nonphagocytic cells and then either establish a protected niche within which they survive and replicate, or disseminate from cell to cell by means of an actin-based motility process. The mechanisms underlying bacterial entry, phagosome maturation, and dissemination reveal common strategies as well as unique tactics evolved by individual species to establish infection. 相似文献
37.
Pascale Pracros F. Fleurat Lessard L. Conan 《Plant foods for human nutrition (Dordrecht, Netherlands)》1983,33(2-3):215-220
Biological assays on white rats and chickens, used to screen the nutritional value of plants proteins, are compared with those of yellow mealworm (Tenebrio molitor L.) and confused flour beetle (Tribolium confusum Duval). With the twenty diets tested, the classification by vertebrates and insect larvae was largely similar. 相似文献
38.
Quantitative kinetic analysis of hydrogen transfer reactions from dietary polyphenols to the DPPH radical 总被引:2,自引:0,他引:2
Diphenylpicrylhydrazyl (DPPH) is widely used for quickly assessing the ability of polyphenols to transfer labile H atoms to radicals, a likely mechanism of antioxidant protection. This popular test generally pays no attention to the kinetics of H atom transfer, which however could be even more important than the total H-atom-donating capacities (stoichiometry, EC50) typically evaluated. In the present work, a series of dietary polyphenols belonging to the most representative families (flavonols from onion, flavanol monomers and oligomers from barley, and caffeic acid and caffeoyl esters from artichoke and endive) are characterized not only by their total stoichiometries (n(tot)) but also by their rate constants of first H atom abstraction by DPPH (k(1)), deduced from the kinetic analysis of the decay of the DPPH visible band following addition of the antioxidant. The mildly reactive DPPH radical allows a good discrimation between polyphenols, as demonstrated by the relatively large ranges of k(1) (ca. 400-5000 M(-)(1) s(-)(1)) and n(tot) (ca. 1-5) values typically measured with antioxidants having a single polyphenolic nucleus. With antioxidants displaying more than one polyphenolic nucleus (procyanidin oligomers, dicaffeoyl esters), the kinetic analysis makes it possible to demonstrate significant differences in reactivity between the subunits (two distinct k(1) values whose ratio lies in the range 3-10) and nonadditive stoichiometries. 相似文献
39.
Some parameters involved in cell neutrality (inorganic solute, cation‐anion balance, proline and organic acid contents linked to oxidative respiration, and PEPCase activity) were determined in salinized plant leaves and leaf calli from two tomato species, the wilty mutant Lycopersicon esculentum cv flacca, and the wild relative L. peruvianum. Callus sensitivity was associated to membrane integrity and cell neutrality; while whole plant sensitivity was linked to nutritional unbalance and energy supply, both factors affecting the maintenance of the cell neutrality by means of ion translocation/compartimentalization. 相似文献
40.
Mathieu M Motte S Ray L Pensis A Jespers P Brimioulle S Laroute V Naeije R Mc Entee K 《American journal of veterinary research》2006,67(7):1236-1243
OBJECTIVE: To investigate the effects of preventive angiotensin converting enzyme inhibitor treatment with ramipril in dogs with progressively severe experimentally induced heart failure. ANIMALS: 20 dogs. PROCEDURES: Dogs were randomly allocated to receive no treatment (control) or ramipril (0.125 mg/kg, PO, daily) for 7 weeks. Physical examination, repetitive catheterization of the right side of the heart, and echocardiography were performed before the study (day 0) and weekly for 7 weeks. Renal plasma flow (RPF) as determined by para-aminohippuric acid clearance and glomerular filtration rate (GFR) as determined by creatinine and iohexol clearances were measured on day 0 and at weeks 4 and 7. RESULTS: Overpacing induced a progressive increase in right atrial pressure (RAP) and pulmonary artery pressure, occluded (PAPO), with a decrease in systemic arterial pressure. There were progressive alterations of echocardiographic indices of diastolic and systolic ventricular function. The RPF and GFR decreased before cardiac output decreased, and filtration fraction increased. The logarithm of the urinary sodium-to-potassium concentration ratio (log(10)[Na(+)/K(+)]) decreased. Significant effects of ramipril included a delay in clinical signs of heart failure, a late decrease in RAP and PAPO, and increases in the sodium excretion fraction and log(10)(Na(+)/K(+)). There was a satisfactory agreement between the creatinine and iohexol clearance measurements. Conclusions AND CLINICAL RELEVANCE: Results suggest that, in this rapid-evolving, dilated cardiomyopathy, activation of the renin-angiotensin system contributes to the pathophysiology of heart failure late in the disease and essentially by an activation of renal salt and water retention. 相似文献