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11.
Garcia-Barros M Paris F Cordon-Cardo C Lyden D Rafii S Haimovitz-Friedman A Fuks Z Kolesnick R 《Science (New York, N.Y.)》2003,300(5622):1155-1159
About 50% of cancer patients receive radiation therapy. Here we investigated the hypothesis that tumor response to radiation is determined not only by tumor cell phenotype but also by microvascular sensitivity. MCA/129 fibrosarcomas and B16F1 melanomas grown in apoptosis-resistant acid sphingomyelinase (asmase)-deficient or Bax-deficient mice displayed markedly reduced baseline microvascular endothelial apoptosis and grew 200 to 400% faster than tumors on wild-type microvasculature. Thus, endothelial apoptosis is a homeostatic factor regulating angiogenesis-dependent tumor growth. Moreover, these tumors exhibited reduced endothelial apoptosis upon irradiation and, unlike tumors in wild-type mice, they were resistant to single-dose radiation up to 20 grays (Gy). These studies indicate that microvascular damage regulates tumor cell response to radiation at the clinically relevant dose range. 相似文献
12.
Seed samples of 174 accessions of pumpkins, squash, and gourds of Cucurbita pepo were measured for length, width, and thickness. Mean seed length ranged from 8.8 to 23.3 mm, mean seed width from 5.0 to 12.5 mm and mean seed thickness from 1.2 to 3.8 mm. The seeds of the edible-fruited cultivar-groups of C. pepo subsp. pepo (Pumpkin, Vegetable Marrow, Cocozelle, Zucchini) were longer, wider, and thicker than those of their counterparts of C. pepo subsp. texana (Acorn, Crookneck, Scallop, Straightneck). The small-fruited, inedible gourds had the smallest seeds. Of the edible-fruited C. pepo subsp. pepo, the Pumpkin Group (round fruits) possessed the longest and flattest seeds whilst the Zucchini Group (long, uniformly cylindrical fruits) possessed the shortest and thickest seeds. The samples tested also differed greatly in mean values for relative dimensions. Length-to-width ratio ranged from 1.5 to 2.5, length-to-thickness ratio from 3.7 to 8.2, and width-to-thickness ratio from 2.0 to 4.9. The Pumpkin Group, followed by the Cocozelle Group (long, bulbous cylindrical fruits) and the Vegetable Marrow Group (short, tapered cylindrical fruits), had seeds of the highest length-to-width ratio. The seeds of the four edible-fruited groups of C. pepo subsp. pepo differed significantly from one another in their length-to-thickness and width-to-thickness ratios. The seeds of the Pumpkin Group and the wild Mexican gourd (C. pepo subsp. fraterna) differed greatly in their dimensions but were most similar to one another in their relative dimensions. In C. pepo, seed size is positively associated with fruit size and seed shape is inversely associated with fruit shape. 相似文献
13.
A. J. Wilson J. Paris A. G. Luckins F. K. Dar A. R. Gray 《Tropical animal health and production》1976,8(1):1-11
Summary Three groups of Boran cattle were maintained under different trypanocidal drug regimes for 29 months in an area of “medium”
tsetse challenge. The development of immunity to trypanosomiasis in the cattle was assessed on the basis of changes in trypanocidal
drug requirement, development of parasitaemia, ability to maintain normal blood values, growth and response to challenge after
the withdrawal of the drugs. Observations were also made on serum antibody content by immunofluorescence and on serum immunoglobulin
levels.
Cattle treated with Berenil on the development of clinical disease developed a partial immunity to trypanosomiasis after two
years. Cattle treated as a group with Berenil on the development of patent parasitaemia in any one animal of the group did
not develop immunity. Cattle treated as a group with Samorin using the same criterion for treatment developed a degree of
immunity to the disease.
It was concluded that group treatment with Samorin was the most suitable of the three regimes examined for the maintenance
of beef cattle in a tsetse infested area.
Resumen Se mantuvieron tres grupos de ganado Boran durante 29 meses bajo differentes regímenes terapéuticos en un área donde la concentración de moscas tsetse era mediana. Se llevó a cabo la evaluación del desarrollo de la inmunidad contra la tripanosomiasis teniendo como base los diferentes cambios en el requerimiento de drogas, desarrollo de parasitemia, abilidad para mantener los valores sanguíneos normales, crecimiento y respuestas a la descarga de parásitos después del retiro de la terápia. Se hicieron también observaciones del nivel de anticuerpos séricos por inmunofluorescencia y se determinó el nivel de inmunoglobulinas. Los animales tratados con Berenil cuando mostraban signos clínicos de la enfermedad, desarrollaron una inmunidad parcial a la tripanosomiasis después de dos a?os. Los animales tratados en grupo con Berenil cuando se detectó parasitemia en cualquier animal del grupo no desarrollaron inmunidad. El ganado tratado con Samorin en base de grupo siguiendo el mismo criterio para el tratamiento, desarrolló cierto grado de resistencia a la enfermedad. Se concluye que el tratamiento de grupo utilizando Samorin fue el mejor de los tres regímenes estudiados para el mantenimiento de ganado de carne en un área infestada de moscas tstse.
Résumé Trois groupes de bovins Boran ont été maintenus vingt neuf mois, sous différents traitements trypanocides, dans une zone “moyennement” infestée de tsé-tsé. Le développement de l'immunité à la trypanosomiase a été évalué à partir des changements dans les besoins en trypanocides, du développement de la parasitémie, de l'aptitude à maintenir les valuers normales du sang, de la croissance et de la réaction à l'infection après la suppression des médicaments. Des observations ont aussi été faites sur la présence d'anticorps sériques par immunofluorescence et sur les teneurs en immunoglobines sériques. Le bétail traité au Berenil à l'apparition de signes cliniques a été partiellement immunisé au bout de deux ans. Dans le groupe du bétail traité au Berenil à l'apparition d'une parasitémie évidente, aucun animal n'a présenté d'immunité. Le groupe de bovins traité au Samorin, selon les nêmes critères a été bien immunisé.相似文献
14.
R. Paris 《Plant foods for human nutrition (Dordrecht, Netherlands)》1967,14(4):376-376
Ohne Zusammenfassung 相似文献
15.
水库水质之时间数列分析研究 总被引:1,自引:0,他引:1
本研究将以德基水库中总磷资料计算 TSI(Trophic Status Index)优养指数,并利用相乘性分解方法(multiplicative decomposition method)进行德基水库水质优养化时间数列分析研究,用以了解德基水库水质之优养化变化情形。研究显示,德基水库季节变化(S)之水质优养化变动情形每年有一高峰期,从 4~10月,总长度达 7个月之久。此外,德基水库优养指数长期趋势(T)乃显示随时间(t)呈缓慢成长状态,其间的关系可表示为 T =51.4231+ 0.0605t,其中 t=1,2,3……,161。至于在循环变动(C)的研究中则发现,循环变动周期自早期的 2~3年,逐渐缩减至 1年左右。由此可知,德基水库水质优养化的情形有日益严重的趋势。 相似文献
16.
Reproductive resilience: a paradigm shift in understanding spawner‐recruit systems in exploited marine fish
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Susan Lowerre‐Barbieri Greg DeCelles Pierre Pepin Ignacio A Catalán Barbara Muhling Brad Erisman Steven X Cadrin Josep Alós Andres Ospina‐Alvarez Megan M Stachura Michael D Tringali Sarah Walters Burnsed Claire B Paris 《Fish and Fisheries》2017,18(2):285-312
A close relationship between adult abundance and stock productivity may not exist for many marine fish stocks, resulting in concern that the management goal of maximum sustainable yield is either inefficient or risky. Although reproductive success is tightly coupled with adult abundance and fecundity in many terrestrial animals, in exploited marine fish where and when fish spawn and consequent dispersal dynamics may have a greater impact. Here, we propose an eco‐evolutionary perspective, reproductive resilience, to understand connectivity and productivity in marine fish. Reproductive resilience is the capacity of a population to maintain the reproductive success needed to result in long‐term population stability despite disturbances. A stock's reproductive resilience is driven by the underlying traits in its spawner‐recruit system, selected for over evolutionary timescales, and the ecological context within which it is operating. Spawner‐recruit systems are species specific, have both density‐dependent and fitness feedback loops and are made up of fixed, behavioural and ecologically variable traits. They operate over multiple temporal, spatial and biological scales, with trait diversity affecting reproductive resilience at both the population and individual (i.e. portfolio) scales. Models of spawner‐recruit systems fall within three categories: (i) two‐dimensional models (i.e. spawner and recruit); (ii) process‐based biophysical dispersal models which integrate physical and environmental processes into understanding recruitment; and (iii) complex spatially explicit integrated life cycle models. We review these models and their underlying assumptions about reproductive success vs. our emerging mechanistic understanding. We conclude with practical guidelines for integrating reproductive resilience into assessments of population connectivity and stock productivity. 相似文献
17.
Laura Righetti Roberta Paris Claudio Ratti Matteo Calassanzio Chiara Onofri Davide Calzolari Wulf Menzel Dennis Knierim Gianmaria Magagnini Daniela Pacifico Gianpaolo Grassi 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(3):575-588
Interveinal chlorosis and leaf margin wrinkling are widespread symptoms of Cannabis sativa. They are traditionally attributed to the so-called hemp streak virus (HSV), but its existence has not been demonstrated yet. To our knowledge, no molecular investigation has so far been performed in order to identify the causal agent of this symptomatology, we therefore decided to use traditional and molecular virology techniques to better characterize symptoms and pursue the etiological agent. No pathogenic virus was found by using targeted PCR reactions and by RNA sequencing, whereas we were able to detect the Cannabis cryptic virus (CanCV) with both techniques. We, therefore, developed an RT-qPCR assay based on a CanCV-specific TaqMan probe and applied it to a wide range of symptomatic and symptomless plants, using a two-step (for quantification), or a one-step (for fast detection) protocol. Both symptoms and the virus were only shown to be transmitted vertically and did not pass via mechanical inoculation or grafting, though we could not find any cause-effect correlation between them. In fact, the virus was found in all the tested hemp samples, and its abundance varied greatly between different accessions and individuals, independently from the presence and severity of symptoms. The suggestion that hemp streak is caused by a virus is therefore questioned. Some abiotic stresses seem to play a role in triggering the symptoms but this aspect needs further investigation. For breeding purposes, a selection of parental plants based on the absence of symptoms proved to be efficient in containment of the disease. 相似文献
18.
Delaying weed adaptation to herbicide by environmental heterogeneity: a simulation approach 总被引:1,自引:0,他引:1
BACKGROUND: Environmental heterogeneity in space or time can drive the evolutionary trajectory of an adaptive trait. This concept could be of practical significance in pesticide resistance management that aims to delay the evolution of a resistance allele. Using a population genetics model, the dynamics of herbicide resistance in a weed species was simulated in a heterogeneous environment with alternation of two unrelated herbicides in time, in space or in both time and space. The level of the environmental heterogeneity (habitat grain) was simulated by a variation in the size of the herbicide-treated areas. RESULTS: The model confirms that several strategies based on habitat heterogeneity efficiently slow down and even prevent resistance evolution. For a recessive resistant trait in outcrossing species, a medium level of environmental heterogeneity (intermediate habitat grain) was found to be the best for delaying herbicide resistance, as previously observed for insecticide resistance management. In selfing species or for a dominant resistant trait in outcrossing species, a low level of environmental heterogeneity (coarse-grained habitat) was more efficient in delaying resistance evolution when heterogeneity in both space and time were considered. CONCLUSION: This model suggests that the choice of optimal tactics for delaying herbicide resistance by enhanced heterogeneity in space or time firstly depends on the interactions between the breeding system and the dominance of the resistance allele in the presence of herbicide, then on the value of the fitness cost and lastly on the dominance of this fitness cost. 相似文献
19.
G Antunes A Chaveiro P Santos A Marques HS Jin F Moreira da Silva 《Reproduction in domestic animals》2010,45(1):26-32
The correlation between apoptosis and early bovine embryonic loss is still not fully elucidated. In the present study, the relationship between the arrest of bovine embryos at the different stages of development and apoptosis was evaluated. We used embryos 7 days after in vitro maturation and fertilization, and morphologic and biochemical apoptotic analyses were performed by using a phase contrast microscope and by the terminal transferase dUTP nick end‐labelling respectively. For the statistic, the apoptotic cell ratio (ACR) was determined as the percentage of apoptotic cells per embryo. To evaluate the relation between ACR and fragmentation pattern, embryos were divided into five groups, groups I–V. To assess the relation between ACR and cytoplasmatic fragmentation, embryos were divided into three groups, according to the fragmentation percentage (<5%; 5–15% and >15%). Of the total 139 embryos included, 65 arrested at 2–8 cells; 14 arrested at 9–16 cells; 18 compacted morula and 42 were non‐arrested blastocysts. The average number of embryonic fragmentation at different stages of the development, 2–8 cells, 9–16 cells, compacted morula and blastocyst, was 16.0 ± 1.5, 28.7 ± 4.4, 4.4 ± 2.4 and 1 ± 0.3 respectively. The embryos at the stage of arrested 9–16 cells and compacted morula had higher ACR than those at the blastocyst stage, excluding the stage of 2–8 cells (the genome is not yet active). The correlation detected between embryonic development and ACR was 0.92 (p < 0.01). It was observed that embryos possessing high fragmentation showed the higher ACR value (r = 0.98, p < 0.05). Comparing the results between fragmentation percentage and ACR, it was observed that the embryos with higher percentage of fragmentation corresponded to higher ACR (r = 0.97, p < 0.01). These results clearly demonstrated that bovine embryonic arrest at different stages of development is correlated with the apoptotic mechanisms. 相似文献
20.
HY Jang YH Kim BW Kim IC Park HT Cheong JT Kim CK Park HS Kong HK Lee BK Yang 《Reproduction in domestic animals》2010,45(6):943-950
Melatonin, the major secretory product of the pineal gland, scavenges a variety of reactive oxygen and nitrogen species in vivo and in vitro, indicating that melatonin is a potent function as an antioxidant. The objective of this study was to investigate the effect of melatonin in the presence or absence of hydrogen peroxide (H2O2) on sperm characteristics (motility, viability, survival rate, membrane integrity, lipid peroxidation (LPO) and mitochondria activity) and also to examine the developmental rates to the blastocysts stage of porcine oocytes fertilized in vitro with semen treated with or without melatonin (100 nm ) in the presence or absence of H2O2 (250 μm ). The sperm were treated with melatonin in the presence or absence of H2O2 for 3, 6, 9 and 12 h at 37°C and then analysed for the sperm characteristics. The porcine embryos were produced by in vitro maturation and in vitro fertilization (IVM/IVF) using semen treated with or without melatonin (100 nm ) in the presence or absence of H2O2 (250 μm ) for 6 h. The semen characteristics, including motility, viability, survival rate, membrane integrity and mitochondria activity, were higher in the groups that were treated with melatonin in comparison to other groups, irrespective of incubation periods. Malondialdehyde levels in control, melatonin and melatonin + H2O2 groups were lower than H2O2 only group. A positive correlation was shown among motility, viability, survival rate and membrane integrity, but a negative correlation was observed between LPO and the other evaluation methods. The developmental rates to blastocysts of IVM/IVF porcine oocytes fertilized by semen treated with melatonin were significantly increased compared with any other groups, with the cell number of blastocysts shown to have a similar trend to the developmental rates. These results demonstrate that melatonin can improve the semen characteristics during in vitro storage and support the developmental ability of IVM/IVF embryos in pigs. 相似文献