布鲁菌S2疫苗株免疫牛与牛种、羊种布鲁菌自然感染牛ELISA鉴别诊断方法的建立

为建立区分猪种布鲁菌S2疫苗株接种奶牛与布鲁菌自然感染奶牛,BLAST比对分析羊种、牛种、猪种、犬种、沙林鼠种和绵羊种6种布鲁菌基因序列,发现repA—related基因是猪种布鲁菌与牛种及羊种布鲁菌的差异基因。设计引物PCR扩增获得repA-related基因片段,克隆并原核表达得到了布鲁菌repA—related融合蛋白,以repArelated蛋白建立间接EI．IsA检测方法。用repA—related蛋白间接ELISA检测猪种s2疫苗株接种动物血清为阳性,检测牛种和羊种布鲁菌自然感染动物血清为阴性。repA—related蛋白间接EusA能从试管凝聚实验（SAT）及常规ELIsA检测阳性的奶牛血清样本中,区分出s2疫苗接种牛与牛种布鲁菌感染牛。     

A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci-infected domestic poultry in Zhejiang Province,China

A cluster of Chlamydia psittaci (C. psittaci) cases was reported in Zhejiang Province, China, 2019. This study evaluates the extent of the outbreak and determines the source of infection. Real-time PCR and sequencing of the ompA gene of C. psittaci were performed to identify the cases, the domesticated poultry and close contacts. The index patient was a 76-year-old woman with chronic vertigo, and Case 2 was a 64-year-old female farmer with herpes zoster. Both women bought psittaci-infected chickens or ducks from the same mobile street vendor and raised them for 10 days and 23 days before fever onset. There were no direct contact between the two women. C. psittaci test was positive for the two patients, one sick chicken, three healthy ducks and the vendor's chicken cage. Phylogenetic analysis showed that all seven C. psittaci positive samples carried identical ompA genotype A of C. psittaci. Of all of the patients' 148 close contacts, none tested positive for C. psittaci, or developed acute respiratory symptoms. Both patients were discharged after a 4-week hospital stay. In conclusion, the source of this cluster was the poultry infected with C. psittaci, which occasionally cause infections in farmers, but inter-human transmission seems unlikely.     

牦牛睾丸附睾LDH同工酶表达模式的研究

利用比色法和聚丙烯酰胺凝胶电泳技术对６、１２、１８、２４月龄半血野牦牛,横交半血野牦牛和家牦牛睾丸、附睾组织ＬＤＨ同工酶活性及谱带表达进行测析。结果表明,睾丸、附睾ＬＤＨ在相同月龄其表达模式不同,６月龄睾丸ＬＤＨ有四条酶谱,活性百分率依次（ＬＤＨ１〉ＬＤＨ３〉ＬＤＨ２〉ＬＤＨ４）附睾只有三条酶谱（ＬＤＨ１〉ＬＤＨ２〉ＬＤＨ３）;到１２、１８月龄时,睾丸ＬＤＨ有五条酶谱（ＬＤＨ１〉ＬＤＨ３〉ＬＤＨ４     

合生素对肉鸭生产性能及胴体品质的影响

选用1日龄健康樱桃谷肉鸭新生雏144只,按体质量随机分为3组,每组设3个重复,每重复16只。对照组饲喂基础日粮,抗生素组添加5mg／kg黄霉素基础日粮,试验组1和组2在基础日粮中分别添加0．05％和0．10％合生素。结果表明,日粮中添加合生素对肉鸭日增质量、胸肌率和腿肌率有显著影响;对肉鸭的饲料转化率有极显著影响;对肉鸭的成活率、屠宰率和皮脂率无显著影响。与抗生素组相比,具有相似的作用。试验组1的日增质量比对照组提高8．56％,比试验组2提高5．54％,比抗生素组提高4．34％;试验组1的料肉比与对照组降低11．52％,比试验组2降低3．29％,比抗生素组降低3．24％。     

Pharmacokinetics of altrenogest in gilts

Altrenogest, a synthetic progestogen, is characterized by its estrus synchronization in mares, ewes, sows, and gilts. To investigate the pharmacokinetic profile and evaluate its accumulation in gilts, 18 oral doses of 20 mg altrenogest/gilt/day were given to eight healthy gilts at an interval of 24 hr. Plasma samples were collected, and altrenogest was determined by ultra‐high‐performance liquid chromatography with mass spectrometry. WinNonlin 6.4 software was used to calculate the pharmacokinetic parameters through noncompartmental model analysis. After the first administration (D 1), the pharmacokinetic parameters, including T_max, C_max, and the elimination half‐life (T_1/2λz), were similar to those observed after the final administration (D 18). However, the mean residence time at D 1 was significantly lower than D 18. As a whole, the mean steady‐state plasma concentration (C_ss), degree fluctuation (DF), accumulation factor (R_ac), and area under the plasma concentration–time curve in steady state (AUC_ss) were 22.69 ± 6.15 ng/ml, 270.64 ± 42.51%, 1.53 ± 0.23, and 544.63 ± 147.49 ng hr/ml, respectively. These results showed that after 18 consecutive days of oral administration of altrenogest, plasma concentrations of altrenogest had a certain degree of fluctuation, without significant accumulations.     

Efficacy of an injectable formulation of eprinomectin against Psoroptes cuniculi, the ear mange mite in rabbits

Thirty rabbits naturally infected with ear mange mite, Psoroptes cuniculi, were subcutaneously administrated with a single dose of eprinomectin at 100, 200 and 300 microg/kg body weight or vehicle on day 0. The extent of lesions was scored on day -6 (prior to treatment), day 0 (treated), days 7, 14, 21, 28 and 35, the ear scabs were collected simultaneously; mites in scabs were examined and counted. The results showed that a single dose of eprinomectin at 200 or 300 microg/kg body weight following subcutaneous administration was able to eliminate P. cuniculi infection in rabbits, and a dose of eprinomectin at 100 microg/kg could significantly reduce mites but was unable to eliminate P. cuniculi.     

N-羟乙酰神经氨酸IgY抗体的制备及鉴定

本试验旨在建立N-羟乙酰神经氨酸(Neu5Gc) IgY抗体的制备及鉴定方法。通过碳二亚胺法将Neu5Gc和载体蛋白进行偶联,制备Neu5Gc人工完全抗原,用制备的完全抗原免疫海兰褐蛋鸡制备Neu5Gc-IgY抗体,经ELISA检测分析鉴定抗体活性。经紫外光谱扫描、琼脂糖凝胶电泳法初步判断Neu5Gc人工完全抗原制备成功,获得的特异性Neu5Gc-IgY抗体经ELISA检测分析,首次免疫后第7天产生抗体,抗体效价呈动态变化,随着免疫次数的增多,血清效价和抗体效价逐步上升,至初次免疫后63 d达到高峰,效价达1:10 000,停止加强免疫后抗体效价可持续一个月左右,1号鸡产生的IgY抗体具有很好的抗原竞争活性,获得竞争回归方程y=30.28x+16.923,线性系数R²=0.9581。以上结果表明,本试验制备的Neu5Gc IgY抗体取得了理想效果,为红肉、牛奶及肿瘤组织中Neu5Gc的检测奠定了基础。     

色氨酸对蛋白质代谢的影响及调控机制

色氨酸作为畜禽的功能性氨基酸,除了作为底物合成蛋白质外,还具有调控蛋白质代谢的功能。本文就色氨酸对蛋白质代谢的影响及调控机制作一综述。     

基于空间Logistic的山西省火灾风险评价与火险区划

利用山西省2002-2012年MODIS卫星遥感数据,建立了描述火灾空间分布及其影响因子的空间Logistic风险模型,从省域空间尺度和较长时间尺度上,开展火险评价与火险区划研究。结果表明,通过空间采样构建的Logistic火灾风险模型拟合效果很好。在显著性水平为0.05的情况下,通过模型系数检验和Wald检验,相对运行特征值(ROC)为0.757。经图层运算得到火烧概率分布图,并将山西省分为无火险区、低火险区、中火险区、高火险区和极高火险区。对山西省火灾风险空间变化影响较为显著的因子为高程(GC)、土地利用类型(LT)、地表温度(LST)、归一化差值植被指数(NDVI)和全球植被湿度指数(GVMI),对火灾风险空间变化影响不显著的因子则仅有坡度(PD)和道路可达性(DL)。NDVI和LST对火灾影响在省内全局显著,而GC、LT和GVMI仅对省内部分地区的火灾影响显著。山西省按火灾影响因素可划分为7类区域,不同类型区域应确定不同的防火重点,制定差别化的防火策略。