农杆菌介导FT基因转化嘎拉苹果的研究

用农杆菌介导法对苹果品种嘎拉转化FT基因进行了研究,建立了苹果离体叶片高效再生体系,在含BA 5mg／L＋IAA 0．5mg／L的MS培养基上,以离体叶片远轴面（叶背面）向上,经过14d的暗培养获得再生不定芽和再生植株,再生率达97％。在转基因再生体系中,以头孢噻肟钠500mg／L和卡那霉素5mg／L,作为脱菌培养和选择培养的条件,经过感染携带拟南芥FT基因的农杆菌,获得了一批转FT基因的植株,经PCR检测,目的基因已经整合到苹果基因组中。     

东北春小麦及部分外引小麦高分子量谷蛋白亚基组成分析

利用SDS-PAGE法分析了72份东北春小麦品种(系)和外引小麦品种HMW-GS组成及分布,结果表明:在Glu-A1位点上的3种变异类型null,1和2*亚基中,东北春小麦1和2*亚基出现的频率最高,均为36.1%,外引品种中null出现的频率最高,为41.7%;在Glu-B1位点上,检测到的6种变异类型(7,6 8,7 8,7 9,14 15,17 18)中7 8亚基类型的出现频率最高,东北春小麦为66.7%,外引品种为44.4%;在Glu-D1位点上的3种变异类型(2 12,2 10,5 10)中5 10出现频率最高,东北春小麦为55.6%,而外引品种高达72.2%。所有参试材料中共出现19种组合类型,其中出现频率最高的是1,7 9,5 10和null,7 8,2 12,均为19.4%。根据Payne的品质评分标准对部分材料进行评定,发现达到10分的材料出现了23份。这些材料可能成为东北春麦区小麦品质改良的宝贵资源。     

凡纳滨对虾不同生长阶段肠道可培养细菌耐药性研究

在凡纳滨对虾(Litopenaeus vannamei)养殖过程中多使用微生态制剂来调节水质,为避免破坏池塘菌群结构,很少使用抗生素.为了解凡纳滨对虾肠道细菌耐药性与不同生长阶段的关系,本研究选取江苏地区4种主要养殖模式凡纳滨对虾成虾和虾苗作为研究对象,利用K-B纸片法和qRT-PCR技术,研究对虾样本肠道可培养细菌对...     

基于模糊控制的温室环境调控系统

以STC12C5410AD系列单片机为核心,采用模糊自整定PID算法,配置以控制电路、环境参数采集电路、键盘显示电路等外围设备,实现对温室环境中温度、湿度、CO_2浓度等参数的实时监测与调控。该系统响应速度快,超调量小,控制精度满足温室控制需要。     

蟋蟀粉替代鱼粉对黄颡鱼幼鱼生长性能、肌肉氨基酸组成和血清生化指标的影响

为研究蟋蟀(Gryllus bimaculatus)粉替代鱼粉对黄颡鱼(Pelteobagrus fulvidraco)幼鱼生长性能、肌肉成分和血清生化指标的影响,以黄颡鱼幼鱼[(2.0±0.13) g]为研究对象,分别使用蟋蟀粉替代0 (对照组)、15%、30%、45%和60%的鱼粉配制成5组等氮等能的实验饲料,分别记为T0、T15、T30、T45和T60组。实验幼鱼在室内循环水系统进行为期10周的养殖实验。结果显示,随着蟋蟀粉替代量的增加,黄颡鱼幼鱼生长呈先增加后降低的趋势;T30组的终末均重(FBW)、增重率(WGR)和特定生长率(SGR)最高,且均显著高于T0组;T30组的饲料系数(FCR)显著低于T0和T15组;T30的肝体比(HSI)显著高于T0和T15,而与T45和T60组无显著差异。与T0组相比,各替代组的脏体比(VSI)、摄食量(FI)、肥满度(CF)和成活率(SR)均无显著差异。黄颡鱼幼鱼肌肉必需氨基酸(EAA)含量中,T60组肌肉中精氨酸(Arg)和缬氨酸(Val)含量显著高于T0组;且蟋蟀粉替代不同比例的鱼粉对黄颡鱼幼鱼肌肉中的总呈味氨基酸(TFAA)含量均未产生显著影响。T30、T45和T60组血清中的葡萄糖(GLU)含量显著高于T0组;相反,其总胆固醇(TCHO)含量显著低于T0组。在本研究条件下,蟋蟀粉替代不同比例的鱼粉不影响黄颡鱼幼鱼的生长性能和肌肉氨基酸含量,且能增加血清GLU和降低TCHO含量,以30%的蟋蟀粉替代鱼粉比例能生长最佳,且优于对照组。本研究对揭示蟋蟀粉在黄颡鱼饲料中替代鱼粉应用的可行性、为今后昆虫蛋白源在水产饲料中的开发提供科学依据。     

Genome-wide association and linkage mapping strategies reveal genetic loci and candidate genes of phosphorus utilization in soybean

Insufficient available phosphorus in soil has become an important limiting factor for the improvement of yield and quality in soybean. The mining of QTLs and candidate genes controlling soybean phosphorus utilization related traits is a necessary strategy to solve this problem. In this study, 11 phosphorus utilization related traits of a natural population of 281 typical soybean germplasms and a recombinant inbred line(RIL) population of 270 lines were evaluated under different phosphorus condit...     

基于美景度评价法和语义分析法的丽江古城桥梁景观美学评价

以丽江古城桥梁景观为研究对象,选取30个桥梁景观单元进行景观美学评价。通过景观评价得出直观视觉与感性形态的综合影响因素,提出相应优化建议,提升丽江古城桥梁景观的美学质量,为丽江古城桥梁旅游路线开发与建设提供科学参考与借鉴。综合运用美景度评价法与语义分析法,构建桥梁景观评价体系,得出影响桥梁景观公众审美偏好的主要因素为植被覆盖率、视野开阔度、环境共生性、桥梁个性度、生态因子、认知因子;根据各桥梁景观单元综合评价得分高低,分析评价者对各景观单元喜好度,提升整体景观美学质量。     

Dissecting the genetic basis of maize deep-sowing tolerance by combining association mapping and gene expression analysis

Deep-sowing is an important method for avoiding drought stress in crop species,including maize.Identifying candidate genes is the groundwork for investigating the molecular mechanism underlying maize deep-sowing tolerance.This study evaluated four traits(mesocotyl length at 10 and 20 cm planting depths and seedling emergence rate on days 6 and 12) related to deep-sowing tolerance using a large maize population containing 386 inbred lines genotyped with 0.5 million high-quality single nucleotide ...     

Change of synapsins in hippocampal formation during spacial learning and memory in rats

AIM and METHODS: The immunohistochemical method was used to study the on synapsin express in hippocampal formation during spatial learning and memory in rats.RESULTS: (1) In control group there are diffuse brown granules without any obvious granular product in hippocampal formation and in model group there are synapsin products in the hippocampal formation ,especially in the subregion CA3 , CA4 and the dentate gyrus after water maze trained for one week. After two weeks water maze trained the hippocampal formation appear the same distribution of the product granules with darker staining. (2) The light dendity of synapsin products between the model and the control groups showed significant differences, that in the model group was highter than that in the control group (P＜0.05). The light dendity of synapsin products increased with the training time from the first week to the second week in the model group. And there is no increase with the training time from the second week to the third week . The light dendity is higher in the CA3, CA4 subregion and the dentate gyrus than the CA1 and CA2 subregion (P＜0.05).CONCLUSIONS: (1) As mentioned above, it is believed that the new synapes are formed in the hippocampal formation of the rat by spacial learning and memory training. (2) The CA3, CA4 subregion and the dentate gyrus are correlated with the spacial learning and memory.     

大白菜甲、乙型核不育系的转育

根 据复等 位基因遗 传假说, 利用已知 基因型 的甲、乙型 雄性 不育“两 用系”,经 过４ 个世代的 杂交选 育,获得了 新甲型“两用系”,临时保持 系和稳 定遗传的 雄性不 育系。