Sparus aurata were cultured during an 8-month period in brackish water (salinity about 25 ppt) in an extensive culture system comprising eight earthen ponds, each with a water surface of 2.1 ha. Initial mean wet weight of fish in all ponds varied from 13.6 ± 1.9 to 19.2 ± 2.6 g/fish. The eight ponds were randomly allocated one of four experimental treatments (two ponds/treatment). In the first treatment, ponds were fertilized monthly with 100 kg urea and 50 kg triple super phosphate. The other treatments (2–4) were fed a locally produced tilapia pellet feed containing 25% crude protein made using different processes. Fish in the second treatment were fed tilapia feed pelleted by compressing machine, whereas in treatments 3 and 4 the pellets were produced by extruder machine (Wenger). Pellets in treatment 3 were floating and in the fourth were semi-sinking. Fish were fed pellets twice daily at 6% of their biomass. The mean final body weight for each treatment respectively was 104.6, 118.9, 156.8 and 158 g. Specific growth rate (SGR) of 0.8, 0.79, 0.99 and 0.95%/day, were obtained in ponds using only inorganic fertilizer, compressed sinking pellets, extruded floating pellets and extruded semi-sinking pellets, respectively. Feed conversion ratios (FCR) for treatments with the extruded tilapia pellets were 2.2 and 2.6 g feed/g gain, which were significantly (P < 0.05) better than treatments with compressed pellets (3.2 g feed/g gain). Production/ha/year were 1389, 1358, 945 and 682 kg fish for the groups fed with extruded floating, extruded semi-sinking, compressed and natural food, respectively. Under the present experimental circumstances, Sparus aurata fed extruded floating tilapia pellets (25% crude protein and 2,600 kcal/g), showed the best productive performance. 相似文献
A traditional Type 3 Basmati rice cultivar grown in India is tall and lodges even under low nitrogen fertilizer dose. In addition
to lodging, it is highly susceptible to several diseases and pests including bacterial blight (BB). BB resistance genes (Xa21 and xa13) and a semidwarfing gene (sd-1) were pyramided in Type 3 Basmati from a rice cultivar PR106-P2 using marker-assisted selection (MAS). Foreground selection
for BB resistance genes, Xa21 and xa13 and reduced plant height gene, sd-1 was carried on the basis of linked molecular markers pTA248, RG136 and ‘h’, respectively. The BC2F3 progenies with both the BB resistance genes were highly resistant with lower lesion length than either of the genes individually.
Background profiling of the selected 16 BC2F3 progenies was done using 95 anchored SSR and 12 ISSR markers. Among the selected 16 BC2F3 progenies, 38-5-2 and 38-5-36 closely clustered along with the recipient parent Type 3 Basmati showing above 85% genetic
similarity with the same. Further selection was continued till F5 generation for higher recovery for Type 3 Basmati characteristics. The desirable alleles of intermediate amylose content
(wx) and aroma (fgr) loci of Type 3 Basmati were also tracked using the linked SSR markers. The BC2F5 pyramid lines T3-4, T3-5, T3-6 and T3-7 homozygous for the three target genes Xa21, xa13 and sd-1 from the donor parent with wx and fgr alleles of Type 3 Basmati had excellent cooking quality and strong aroma. 相似文献
Four commercial cultivars of potatoes were maintained under normal storage conditions at 44 F for 34 weeks. Except for a final 10 week interval tubers were withdrawn at 6 week intervals. After slicing, a portion of the slices was immediately analyzed for total glycoalkaloid content. The remaining slices were aged for four days in the dark at room temperature, then similarly analyzed. The total glycoalkaloid content of the aged slices increased dramatically on aging. This increase on aging of slices reached a maximum early in storage then decreased gradually over the storage period. In determining the individual glycoalkaloids, α-solanine and α-chaconine both increased in these slices, but the greatest increase was in the former. Appearing solely in the aged slices of the Kennebec variety, α-and β-solamarine appeared early in the storage period and gradually decreased over the storage period. Analyses of the unaged slices indicated that the glycoalkaloid content and composition of the potato tubers was little affected by storage. Aging of potato sprouts did not change their glycoalkaloid content. 相似文献
In this study some bioactive properties of extracts of wild pear (Pyrus elaegnifolia), hawthorn (Crataegus spp.), oriental hackberry (Celtis tournefortii), oleaster (Elaeagnus angustifolia), Japanese crabapple (Malus floribunda), rosehip (Rosa canina), pedunculate oak (Quercus robur), service tree (Sorbus domestica) and firethorn (Pyracantha coccinea) wildly growing in Turkey were investigated. The fruit extracts were analysed in terms of total phenolic content (TPC), antioxidant activities (DPPH and phosphomolybdenum methods) and antimicrobial activity. In the results, TPC, IC50 value of DPPH and IC50 value of phosphomolybdenum for pedunculate oak were 454.35?mg GAE/g d.w., 340.52?mg AAE/g d.w. and 96.52%, respectively. Service tree and firethorn exhibited the lowest TPC (4.11?mg GAE/g d.w.) and IC50 value of DPPH (53.32?mg AAE/g d.w.), respectively. Moreover, antimicrobial activity of oleaster was 2.46%. The hackberry fruit extract had no inhibitory effect against the tested microorganisms whereas pedunculate oak extract (with 10%) had the highest inhibitory effect against Aeromonas hydrophila. Additionally, all the fruit extracts had no antimicrobial activity against the Saccharomyces cerevisiae. In conclusion, wild edible fruit extracts can be used as bioactive material due to their antioxidant and antimicrobial activity in food industry and human nutrition.
European Journal of Plant Pathology - Chili anthracnose, caused by Colletotrichum species is a major disease of chili leading to severe economic loss worldwide. Here, two new species-specific... 相似文献
A method has been developed for the quantitative analysis of total glycoalkaloids (TGA) in potato tubers. The method consists of TGA extraction by suitable solvent mixtures followed by hydrolysis of the glycosides and extraction of the aglycones. The aglycones are then quantitated by nonaqueous titration. The advantages of this method over those previously described are the inclusion of glycoalkaloids that are not measured by other methods, and the simplicity, safety, and rapid nature of the procedure. This method has been applied to the TGA analysis of potato tubers subjected to a variety of storage and treatment conditions. 相似文献
Zusammenfassung Es wurden Labortests zur Klärung der Frage durchgeführt, wieweit der räuberische NematodeDiplogaster sp. in der Lage ist, die Dichte der Larven der zwei wurzelparasitären NematodenMeloidogyne javanica undTylenchulus semipenetrans zu reduzieren. Eine weitere Versuchsserie verwendete mit sterilem Boden gefüllte Blumentöpfe.Die Ergebnisse zeigen, daß der Raub-Nematode beide parasitischen Nematodenarten,M. javanica an Tomaten- undT. semipenetrans an Orangen-Wurzeln stark reduzierte. Dabei erwies sich die Wirkung unabhängig von der Beuteart aber abhängig von der Beutedichte.
Studies on the potential use of the predator, Diplogaster sp. (Nematodes, Diplogasteroidea) on certain root-parasitic Nematodes
A laboratory test was conducted in glass petri dishes to assess the efficacy ofDiplogaster sp. on the predation of the second stage larvae ofMeloidogyne javanica andTylenchulus semipenetrans nematodes at 25°C and 65% R. H. in the incubator. In addition, an applied experiment in pots was carried out to explore the role of the predator on the two parasitic nematode genera.
Diplogaster sp. exteriminated large numbers of prey ofM. javanica andT. semipenetrans larvae 7 days after the beginning of the experiment. The rate of predation on parasitic nematodes in laboratory test was independent on the prey species but dependent on the prey density. The parasitic nematodes,M. javanica andT. semipenetrans, populations in the roots of tomato and sour orange, respectively, were significantly reduced by the addition of the predatory nematode,Diplogaster sp., in the pot experiment.
The molluscicidal activity of Thuricide used as bacterial insecticide,Bacillus thuringiensis, againstBiomphalaria alexandrina andBulunus truncatus was tested.The obtained results indicate that Thuricide possess a mollus-cicidal activity against both snails species.B. truncatus was more sensitive to the action of Thuricide. However the slope function of the LC50 forB. alexandrina was significantly higher than that forB. truncatus. The mortality rate of exposedB. alexandrina increased by increasing the exposure time.Exposure ofB. alexandrina to low concentration of Thuricide caused a significant decrease in both ovipository activity and size of egg masses and reduced the percentage of egg hatchability.
Zusammenfassung Es wurde die molluscizide Wirkung von Thuricide (Bac. thuringiensis) gegen die SchneckenBiomphalaris alexandrina undBulunus truncatus getestet. Die Ergebnisse zeigen, daß Thuricide für beide Schnecken molluscizid war.B. truncatus erwies sich als empfindlicher, doch war die Neigungs-Funktion des LC50-Wertes fürB. alexandrina signifikant höher als fürB. truncatus. Die Mortalitätsrate vonB. alexandrina erhöhte sich mit zunehmender Expositionszeit. Die Exposition vonB. alexandrina gegenüber geringen Konzentrationen von Thuricide verursachte eine signifikante Verringerung sowohl der Eiablage-Aktivität als auch der Größe der Eigelege sowie des Schlüpfprozents der Eier.
Zusammenfassung Baumwolle, Weizen, Mais, Sojabohne, Zwiebel, Kichererbse und Sesam wurden hinsichtlich ihrer Anfälligkeit gegen die WurzelnematodenMeloidogyne javanica undM. incognita incrita untersucht. Sesam und Mais waren gegen beide Nematodenarten resistent. Baumwolle wurde vonM. javanica nicht befallen. Dagegen wurden Zwiebel und Sojabohne von diesem Nematoden stark geschädigt. Die statistische Analyse zeigte signifikante Befallsunterschiede zwischen den Wirtspflanzen undMeloidogyne-Arten.
Susceptibility of certain field crops to some Nematoda infection, Meloidogyne javanica (Treub) and M. incognita acrita (Kofoid & White) chitwood
Varietal susceptibility of certain field crops were tested. Sesame and maize were resistant toM. javanica andM. incognita acrita infection. Cotton was resistant toM. javanica only. Onion and soybean were highly infected withM. javanica in comparison with the other tested hosts. Soybean also was highly infected withM. incognita acrita. Wheat showed the presence of the lowest of nematode population of the two species on its roots. Severity of galls was paralleled to the mentioned nematode populations.Statistical analysis of the data indicates that there were significant differences between the tested hosts. Also, the interaction between nematode species and hosts was significant.