Plague bacteria target immune cells during infection

The plague is caused by the bacterium Yersinia pestis. Plague bacteria are thought to inject effector Yop proteins into host cells via the type III pathway. The identity of the host cells targeted for injection during plague infection is unknown. We found, using Yop beta-lactamase hybrids and fluorescent staining of live cells from plague-infected animals, that Y. pestis selected immune cells for injection. In vivo, dendritic cells, macrophages, and neutrophils were injected most frequently, whereas B and T lymphocytes were rarely selected. Thus, it appears that Y. pestis disables these cell populations to annihilate host immune responses during plague.     

On-farm impacts of zero tillage wheat in South Asia's rice–wheat systems

The recent slow down in productivity growth in the irrigated areas of the Indo-Gangetic Plains of South Asia has led to a quest for resource-conserving technologies that can reduce production costs, save water and improve production. Findings from farm surveys are used to evaluate the on-farm impacts of zero tillage (ZT) wheat in the rice–wheat systems of India's Haryana state and Pakistan's Punjab province. ZT-induced effects primarily apply to the establishment and production costs of the wheat crop. Both study sites confirmed significant ZT-induced resource-saving effects in farmers’ fields in terms of diesel, tractor time and cost savings for wheat cultivation. Water savings are, however, less pronounced than expected from on-farm trial data. It was only in Haryana, India that there were significant ZT-induced water savings in addition to significant yield enhancement. The higher yield and water savings in Haryana, India result in significantly higher water productivity indicators for ZT wheat. In both sites, there are limited implications for the overall wheat crop management, the subsequent rice crop and the rice–wheat system as a whole. The combination of a significant “yield effect” and “cost-saving effect” makes adoption worthwhile and provide a much needed boost to the returns to wheat cultivation in Haryana, India. In Punjab, Pakistan, ZT is primarily a cost-saving technology for wheat cultivation. The prime driver for ZT adoption is not water savings or natural resource conservation but monetary gain in both sites. Water savings are only a potential added benefit.     

The effect of three anaesthetic protocols on the stress response in cane toads (Rhinella marina)

ObjectiveThree anaesthetics (MS222, clove oil and a mixture of ketamine/diazepam) were administered to cane toads to determine their effect on the hypothalamic-pituitary-adrenal (HPA) axis. Time to induction and recovery and any adverse events were also evaluated.Study designProspective randomized experimental trial.AnimalsThirty adult male cane toads (Rhinella marina) with body mass ranging between 130 and 250 g were captured from the field.MethodsThree groups of 10 toads were anaesthetized with ketamine (200 mg kg^?1) and diazepam (0.2 mg kg^?1) by intramuscular injection, MS222 (3 g L^?1) or clove-oil (0.3 mL L^?1) both by immersion. Blood samples were collected to determine plasma corticosterone concentrations. Induction and recovery time were recorded in each treatment. After full recovery animals were euthanized and a complete post-mortem examination was performed.ResultsSignificant differences were found in the activation of the HPA axis and in the times of induction and recovery between treatments (p < 0.001). Animals anaesthetized with clove-oil had the highest levels of corticosterone in plasma (42.5 ± 21.6 ng mL^?1). No differences were found between ketamine/diazepam (15.0 ± 13.3 ng mL^?1) and MS222 (22.0 ± 13.6 ng mL^?1) groups. The mean ± SD induction (minutes) and recovery (hours) times respectively were; ketamine/diazepam 66.5 ± 11 and 8 ± 3, clove oil 39 ± 12 and 7.6 ± 3, and MS222 42.5 ± 11 and 1.5 ± 0.5. Clove oil exposure had 30% mortality. Death followed a period of respiratory distress with changes consistent with non-cardiogenic oedema observed at post-mortem examination.Conclusions and Clinical relevanceBased on shorter induction and recovery times and minimal activation of HPA, MS222 is the anaesthetic of choice in cane toads. If it is not possible to use immersion methods of anaesthesia, ketamine/diazepam can be used but induction and recovery times are prolonged. Clove oil had unacceptable mortality in this study and should be used with extreme caution.