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71.
The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding. Hunong 5 has high commercial value due to its high polysaccharide and triterpene content. This is the first report of using a DNA pooling method to develop a stable sequence characterized amplified region (SCAR) marker for rapid identification of the G. lucidum Hunong 5 cultivar. The SCAR marker was developed by first generating and sequencing a distinctive inter simple sequence repeat (ISSR) fragment (882 bp) from G. lucidum Hunong 5 cultivar. A stable SCAR primer pair GLH5F/GLH5R were obtained to identify the cultivar and the SCAR marker is a DNA fragment of 773 bp. 相似文献
72.
Wind‐induced stock variation of the neon flying squid (Ommastrephes bartramii) winter–spring cohort in the subtropical North Pacific Ocean
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Haruka Nishikawa Takahiro Toyoda Shuhei Masuda Yoichi Ishikawa Yuji Sasaki Hiromichi Igarashi Mitsuo Sakai Masaki Seito Toshiyuki Awaji 《Fisheries Oceanography》2015,24(3):229-241
Our examination of the neon flying squid (Ommastrephes bartramii) winter–spring cohort catch per unit effort (CPUE, an index of stock) revealed significant positive correlations with the interannual variations of observed chlorophyll‐a (Chl‐a) concentration and autumn–winter mixed layer depth (MLD) in the winter–spring feeding grounds of paralarvae and juveniles (130–170°E, 20–27°N). These correlations suggest the importance of integrated bottom‐up effects by the autumn–winter MLD for the neon flying squid stocks. However, the influence of autumn–winter MLD interannual variation in the forage availability for paralarvae and juveniles, i.e., particulate organic matter and zooplankton, has still been unclear. In this study, we use the lower trophic ecosystem model NEMURO, which uses the physical environmental data from the ocean reanalysis dataset obtained by the four‐dimensional variational (4DVAR) data assimilation method. The model‐based investigation enables us to clarify how the autumn–winter MLD controls the particulate organic matter and zooplankton abundance in the feeding grounds. Further, our investigation of the autumn–winter MLD interannual variation demonstrates that the stronger autumn wind in the feeding grounds develops a deeper mixed layer. Therefore, the deep mixed layer entrains nutrient‐rich water and enhances photosynthesis, which results in good feeding conditions for paralarvae and juveniles. Our results underline that the wind system interannual variation has critical roles on the winter–spring cohort of the neon flying squid stock. 相似文献
73.
Kumiko Hayashi Kunihiko Konno Kyoko Adachi Takeshi Nawamura Yoshikazu Shizuri Yuji Okita Ikuo Kimura 《Fisheries Science》2009,75(3):797-803
Suppressive effects of neocarrabiose 4-O-sulfate on the thermal and freeze denaturation of myosin subfragment-1 (S-1) and on myofibrils were investigated. The compound
strongly suppressed the thermal denaturation of S-1. Its suppressive effect was greater than that of sorbitol and similar
to that of maltose. However, it tended to accelerate the denaturation of myofibrils, suggesting a loss of protection by F-actin
upon its addition. The compound suppressed the freeze denaturation of myofibrils and S-1. The effect was similar to that of
sorbitol or maltose, and completely different from that of Na-sulfate. The compound solubilized myofibrils at concentrations
similar to KCl. Therefore, it was concluded that neocarrabiose 4-O-sulfate behaved as an ionic salt in the thermal treatment process, whereas it behaved as a sugar in the frozen storage process. 相似文献
74.
75.
Yoichi INOUE Tetsushi ONO Keiichi HISAEDA Yutaka YAMADA Akihisa HATA Takako Shimokawa MIYAMA Kenichi SHIBANO Emi OHZAWA Hitoshi KITAGAWA Eri IWATA 《Journal of Equine Science》2022,33(2):27
The objective of this study is to analyze the relationships between the age and blood test results or body sizes in Noma horses by using the results of periodical health examination. Out of 45 hematological or physical items examined, statistically significant, but loose correlations were observed in 14 items. Red blood cell count, activities of aspartate aminotransferase, alkaline phosphatase, and creatinine kinase, concentrations of calcium and inorganic phosphorus decreased with aging. Conversely, mean corpuscular volume, mean corpuscular hemoglobin, lipase activity, γ-globulin and chloride concentrations, body height, chest circumference and cannon bone circumference increased with aging. The changes in a few items seemed unique to Noma horse. However, most age-related changes found in this study might be considered as a common trend in horse breeds rather than distinctive characteristic in Noma horse. 相似文献
76.
Methane uptake and nitrous oxide emission in Japanese forest soils and their relationship to soil and vegetation types 总被引:1,自引:1,他引:1
Tomoaki MORISHITA Tadashi SAKATA Masamichi TAKAHASHI Shigehiro ISHIZUKA Takeo MIZOGUCHI Yoshiyuki INAGAKI Kazuhiko TERAZAWA Satoshi SAWATA Masanori IGARASHI Hiroshi YASUDA Yasuhiro KOYAMA Yoshihito SUZUKI Nobuyuki TOYOTA Masamichi MURO Masaru KINJO Hirokazu YAMAMOTO Daitaro ASHIYA Yoichi KANAZAWA Tetsu HASHIMOTO Hidetaka UMATA 《Soil Science and Plant Nutrition》2007,53(5):678-691
77.
Masakatsu NOHARA Keiichi HISAEDA Tetsushi ONO Yoichi INOUE Kouji OGAWA Akihisa HATA Kenichi SIBANO Hajime NAGAHATA Noboru FUJITANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(7):964
There has been an increase in temperature and the incidence of extreme weather events, such as heat wave, due to global warming, which has promoted the incidence of livestock diseases. Therefore, it is important to examine the effect of changes in environmental parameters on livestock performance. The aim of this study was to examine the relationship between ambient environmental conditions in livestock pen and the physiological parameters of Holstein dairy cows. The results showed that there was a decrease in the red blood cell counts, hemoglobin concentrations, and mean corpuscular hemoglobin concentration of the cows with increasing pen temperature, wet bulb globe temperature (WBGT), and temperature humidity index (THI). Additionally, high daily variation in temperature caused a decrease in the serum albumin levels of the cows. Moreover, the lowest serum calcium, inorganic phosphorus, and magnesium concentrations were observed in November, and were negatively correlated with the 24-hr temperature, WBGT, and THI range of the pen prior to sampling. Multiple regression analysis showed a positive correlation between serum cortisol concentration and 24-hr WBGT range of the pen prior to samplings and packed cell volume. However, serum cortisol and total protein concentrations were negatively correlated. Overall, the findings of the study suggest that large variation in temperature induced stress in the cows, which could be overcome by increased water consumption and improved protein digestion and absorption by the animals, and the addition of minerals, such as calcium to the diet. 相似文献
78.
79.
Molecular sexing of Japanese cormorants used for traditional fishing on the Nagara River in Gifu City 总被引:2,自引:0,他引:2
Miho INOUE-MURAYAMA Yuko UEDA Tetsuji YAMASHITA Chizuko NISHIDA-UMEHARA Yoichi MATSUDA Toshiaki MASEGI Shin'ichi ITO 《Animal Science Journal》2002,73(5):417-420
The Japanese cormorants used in traditional fishing in Japan are wild derived and their sex cannot be determined from their appearance. Applicability of molecular sex determination based on the size difference between CHD1Z and CHD1W introns was confirmed in male and female Japanese cormorants whose sexes had been ascertained by pathological autopsy. All of 21 birds of unknown sex reared by a cormorant fishing master were identified as males. The molecular sexing method will provide valuable information on sex differences of wild Japanese cormorants, including tameness, trainability, behavior and fishing capability, as well as for future trials involving artificial reproduction. 相似文献
80.
Mikiko FUKUDA Mayuko INOUE Daisuke MURAMATSU Hitoshi MIYACHI Yoichi SHINKAI 《The Journal of reproduction and development》2016,62(1):121-125
Production of knockout mice using targeted embryonic stem cells (ESCs) is a powerful approach for
investigating the function of specific genes in vivo. Although the protocol for gene
targeting via homologous recombination (HR) in ESCs is already well established, the targeting efficiency
varies at different target loci and is sometimes too low. It is known that knockdown of the Bloom syndrome
gene, BLM, enhances HR-mediated gene targeting efficiencies in various cell lines. However,
it has not yet been investigated whether this approach in ESCs is applicable for successful knockout mouse
production. Therefore, we attempted to answer this question. Consistent with previous reports,
Blm knockdown enhanced gene targeting efficiencies for three gene loci that we examined by
2.3–4.1-fold. Furthermore, the targeted ESC clones generated good chimeras and were successful in germline
transmission. These data suggest that Blm knockdown provides a general benefit for efficient
ESC-based and HR-mediated knockout mouse production. 相似文献