全文获取类型
收费全文 | 4439篇 |
免费 | 267篇 |
国内免费 | 10篇 |
专业分类
林业 | 213篇 |
农学 | 239篇 |
基础科学 | 48篇 |
738篇 | |
综合类 | 333篇 |
农作物 | 189篇 |
水产渔业 | 208篇 |
畜牧兽医 | 2199篇 |
园艺 | 110篇 |
植物保护 | 439篇 |
出版年
2021年 | 40篇 |
2020年 | 78篇 |
2019年 | 61篇 |
2018年 | 84篇 |
2017年 | 100篇 |
2016年 | 81篇 |
2015年 | 62篇 |
2014年 | 83篇 |
2013年 | 196篇 |
2012年 | 169篇 |
2011年 | 223篇 |
2010年 | 180篇 |
2009年 | 166篇 |
2008年 | 205篇 |
2007年 | 174篇 |
2006年 | 170篇 |
2005年 | 163篇 |
2004年 | 145篇 |
2003年 | 147篇 |
2002年 | 166篇 |
2001年 | 131篇 |
2000年 | 117篇 |
1999年 | 107篇 |
1998年 | 52篇 |
1997年 | 34篇 |
1996年 | 40篇 |
1995年 | 37篇 |
1994年 | 46篇 |
1993年 | 55篇 |
1992年 | 83篇 |
1991年 | 74篇 |
1990年 | 79篇 |
1989年 | 72篇 |
1988年 | 43篇 |
1987年 | 60篇 |
1986年 | 47篇 |
1985年 | 56篇 |
1984年 | 61篇 |
1983年 | 51篇 |
1982年 | 53篇 |
1980年 | 33篇 |
1979年 | 39篇 |
1978年 | 45篇 |
1977年 | 44篇 |
1976年 | 43篇 |
1975年 | 34篇 |
1974年 | 41篇 |
1972年 | 34篇 |
1971年 | 34篇 |
1969年 | 42篇 |
排序方式: 共有4716条查询结果,搜索用时 15 毫秒
91.
R. Barba-Gonzalez K.-B. Lim M. S. Ramanna R. G. F. Visser J. M. Van Tuyl 《Euphytica》2005,143(1-2):67-73
Cytological modes of the origin of 2n gametes were investigated in six different genotypes of F1 hybrids between Oriental and Asiatic (OA) lilies (Lilium, 2n = 2x = 24). Chromosome pairing between the parental genomes was very low, the average frequency range from 0.3 to 1.2 bivalents per cell among the genotypes. Within a genotype the frequency of bivalents varied from 0 to 6 in some cases. The normally occurring haploid pollen grains were totally sterile. In contrast, in different genotypes, variable percentages of 2n pollen were found and shown to be fertile as estimated from pollen germination. A cytological analysis of Metaphase I and subsequent stages of meiosis using genomic in situ hybridization (GISH) revealed that there was intergenomic recombination between the alien genomes. Following Metaphase I stage, three different types of abnormal cytological events led to the formation of 2n pollen: (i) Post-Metaphase I division (PMI), (ii) Post-Metaphase II division (PMII) and (iii) Asymmetric Cytokinesis of the pollen mother cell followed by chromosome division. All three cytological events led to first division restitution (FDR) gametes. Based on in vitro pollen germination it was proved for two genotypes that 2n pollen was viable only during the first day of anthesis. It was possible to use 2n pollen successfully for backcrossing. Implications of 2n pollen for intergenomic recombination in BC1 progenies are discussed. 相似文献
92.
Influence of fertilization on acrylamide formation during frying of potatoes harvested in 2003 总被引:3,自引:0,他引:3
De Wilde T De Meulenaer B Mestdagh F Govaert Y Vandeburie S Ooghe W Fraselle S Demeulemeester K Van Peteghem C Calus A Degroodt JM Verhé R 《Journal of agricultural and food chemistry》2006,54(2):404-408
The quality of the potato has been found to vary, when grown under different agricultural and environmental conditions, such as the level of fertilization. Consequently these factors may influence the acrylamide formation during the preparation of French fries. These assumptions were studied on three varieties: Bintje, Ramos, and Saturna from the harvest of 2003. Decreasing N fertilization caused increases in the reducing sugar concentration from 60% up to 100% on DM for all varieties studied. Due to a high correlation between the reducing sugar content and the generation of acrylamide during frying, this resulted in a parallel increase in the acrylamide concentration of the French fries. Thus by lowering the amount of N fertilizer, an increase of 30-65% of the acrylamide generation during frying could be observed. It seems of extreme importance to find an appropriate balance between the level of N fertilizer in order to diminish acrylamide formation but on the other hand to obtain an acceptable tuber and to consider the environmental impact. All results reported should be seen in the perspective of the warm growing season of 2003. 相似文献
93.
Timbermont L Lanckriet A Gholamiandehkordi AR Pasmans F Martel A Haesebrouck F Ducatelle R Van Immerseel F 《Comparative immunology, microbiology and infectious diseases》2009,32(6):503-512
Since the ban on growth-promoting antibiotics in animal feed in the European Union, necrotic enteritis has become a major cause of mortality in broiler chickens. Despite the importance of the disease, the pathogenesis is still not completely understood. In the current study, Clostridium perfringens strains isolated from healthy flocks and isolates from outbreaks of necrotic enteritis were evaluated for the ability to cause gut necrosis in an intestinal loop model in laying hens and in an experimental infection model in broilers. High, intermediate and low alpha toxin producing strains were chosen from each isolation source. Only the isolates from field outbreaks induced necrotic gut lesions, independent of the amount of alpha toxin produced in vitro. It was also shown that alpha toxin producing isolates from calf hemorrhagic enteritis cases were not able to induce necrotic enteritis in poultry. These results suggest the presence of host specific virulence factors in C. perfringens strains, isolated from chickens with intestinal necrotic enteritis lesions. 相似文献
94.
Elena Russold Tamas D Ambrisko Johannes P Schramel Ulrike Auer Rene Van Den Hoven Yves P Moens 《Veterinary anaesthesia and analgesia》2013,40(1):48-54
ObjectiveTo compare tidal volume estimations obtained from Respiratory Ultrasonic Plethysmography (RUP) with simultaneous spirometric measurements in anaesthetized, mechanically ventilated horses.Study designProspective randomized experimental study.AnimalsFive experimental horses.MethodsFive horses were anaesthetized twice (1 week apart) in random order in lateral and in dorsal recumbency. Nine ventilation modes (treatments) were scheduled in random order (each lasting 4 minutes) applying combinations of different tidal volumes (8, 10, 12 mL kg?1) and positive end-expiratory pressures (PEEP) (0, 10, 20 cm H2O). Baseline ventilation mode (tidal volume = 15 mL kg?1, PEEP = 0 cm H2O) was applied for 4 minutes between all treatments. Spirometry and RUP data were downloaded to personal computers. Linear regression analyses (RUP versus spirometric tidal volume) were performed using different subsets of data. Additonally RUP was calibrated against spirometry using a regression equation for all RUP signal values (thoracic, abdominal and combined) with all data collectively and also by an individually determined best regression equation (highest R2) for each experiment (horse versus recumbency) separately. Agreement between methods was assessed with Bland-Altman analyses.ResultsThe highest correlation of RUP and spirometric tidal volume (R2 = 0.81) was found with the combined RUP signal in horses in lateral recumbency and ventilated without PEEP. The bias ± 2 SD was 0 ± 2.66 L when RUP was calibrated for collective data, but decreased to 0 ± 0.87 L when RUP was calibrated with individual data.Conclusions and clinical relevanceA possible use of RUP for tidal volume measurement during IPPV needs individual calibration to obtain limits of agreement within ± 20%. 相似文献
95.
Parker JE Timm KI Smith BB Van Saun RJ Winters KM Sukon P Snow CM 《American journal of veterinary research》2002,63(7):948-953
OBJECTIVE: To evaluate temporal changes in bone mineral density associated with seasonal variation in serum vitamin D, calcium, and phosphorus concentrations in alpacas. ANIMALS: 5 healthy mature neutered male alpacas. PROCEDURE: Metacarpal bone mineral density was measured at 4 times during a year. Each time alpacas were weighed, blood was collected for determination of serum calcium, phosphorus, and vitamin D concentrations, and samples of feed were analyzed for nutrient content. Vitamin D status was determined by use of an assay that measured serum 25-hydroxycalciferol concentration. Effects of changes in serum vitamin D, calcium, and phosphorus concentration and body weight with season on bone mineral density were determined. RESULTS: Bone mineral density, body weight, and serum vitamin D and phosphorus concentrations varied with season. Bone mineral density, serum vitamin D concentration, and body weight also varied among individual alpacas. Serum vitamin D concentration was lower in January than the previous October and increased from May to the following September. The decrease in bone mineral density lagged behind the decrease in serum vitamin D concentration and was lower in May, compared with the previous October. Body weight was lower in May than the previous October or following September. Solar radiation was highest in July and lowest in December. CONCLUSIONS AND CLINICAL RELEVANCE: Seasonal changes in bone mineral density are associated with changes in serum vitamin D concentrations in alpacas. Changes in bone mineral density associated with a decline in serum vitamin D concentration may predispose some alpacas to developing fractures minimal trauma. 相似文献
96.
Jadwiga W. Płodowska Peter H. J. Jongebloed Pieter A. C. M. Van De Sanden Paul C. Struik 《Potato Research》1989,32(3):255-266
Summary Single-stemmed potato plants, cv. Bintje, were grown on a nutrient solution under controlled conditions. They were exposed
to a short drought stress, or remained untreated. Short-term changes in tuber volume and in specific leaf weight were measured
non-destructively.
Tubers from control plants increased rapidly in volume during the night and did not grow, or even shrivelled during the day.
Tubers of stressed plants did not show a uniform response; some tubers markedly declined in volume during the day. The patterns
of volume increase of stressed plants became erratic after the stress, mainly because some plants never fully recovered.
Patterns of diurnal fluctuation in tuber volume were similar to diurnal changes in specific leaf weight; tubers within the
same stressed plant sometimes reacted differently. In these cases the effects on the diurnal fluctuation partly reflected
the long-term effects, reported in an earlier paper. 相似文献
97.
Summary Based on visual inspection, discrimination between common scab (Streptomyces spp.) and powdery scab (Spongospora subterranea) can be difficult. Inspections are performed on unwashed samples, incidentally supported by microscopic examination.
During 1994–1996 surveys were performed in The Netherlands on tubers with symptoms resembling common scab. Under microscopic
assessment nearly all samples showed the presence of structures resembling cystosori (sporeballs) ofS. subterranea. At that time confirmation using alternative techniques was not possible.
In 2003 research was undertaken to clarify the situation with respect to scab on potato tubers in The Netherlands. One hundred
and eighteen scab samples were extensively tested forS. subterranea. All samples were digitally photographed, microscopically examinated and tested with real-time PCR and DAS-ELISA. Use of
these modern methods resulted in a clear picture of symptoms that can be attributed toS. subterranea. A lot of scab samples with structures resembling cystosori could not be confirmed as contaminated withS. subterranea. 相似文献
98.
The use of Contans (Coniothyrium minitans) in Belgian commercial lettuce greenhouses has so far not always fulfilled the expectations of growers. With the aim of improving the efficacy of control of lettuce drop (Sclerotinia sclerotiorum), Contans applied alone and Contans combined with Radix (Trichoderma asperellum and Trichoderma gamsii), Kraft pine lignin and Rovral was compared in three successive lettuce crops in a naturally infested greenhouse. At harvest of each crop, the effect on disease incidence, and the effect on viability and fungal infection of sclerotia buried prior to each crop was evaluated. Contans applied alone negatively affected the sclerotial viability, but did not reduce lettuce drop symptoms. The combination of Contans with Kraft pine lignin, on the other hand, reduced sclerotial viability and lettuce drop compared with the untreated control in the last crop. Furthermore, when Contans was combined with Kraft pine lignin, a higher number of infected sclerotia was observed compared with Contans applied alone. When Radix was added to the combination, there was no extra benefit for the control of lettuce drop. The combination of one Rovral application and Contans resulted in a significant control of lettuce drop together with a negative effect on sclerotial survival. Two Rovral applications, by contrast, reduced the ability of C. minitans to parasitize and kill the sclerotia. To conclude, integration of Contans with a reduced Rovral application and with Kraft pine lignin has potential to improve the control of lettuce drop in commercial greenhouses. 相似文献
99.
Summary From 1985 to 1987 field experiments were caried out to test the influence of different pre-planting storage treatments on
yield and size distribution of potato crops. The storage treatments included 4, 8 and 12°C in the light and in the dark, for
different periods before planting. Subjecting the seed tubers to light at low temperatures induced crops to produce a larger
proportion of smaller sized tubers while storing seed tubers at higher temperatures in the dark led to an increase of tubers
>55 mm. These observations were confirmed in five field experiments with two cultivars at two sites and in three growing seasons. 相似文献
100.
Tran Van Minh Nguyen Van Uyen Peter Vander Zaag 《American Journal of Potato Research》1990,67(11):779-787
Potato production from rooted apical cuttings or from small tubers (l–20g) produced in high density beds has great potential in Asia. This is particularly important in non-traditional warmer environments. This research was conducted to assess the feasibility of producing many small tubers in high density beds. A second objective compared tubers from beds with transplanted rooted cuttings and traditional seed tubers for growth and yield in the field. Tuberlet production in beds exceeded 2000/m2 with a density of 1000 plants/m2 under optimal growing conditions while under high temperatures only about 1.25 tuberlets/plant were produced. In field experiments, transplanted cuttings yielded 20 t/ha in the mid and high elevation areas, only slightly less than tuberlets of 5–10g while at the hot site, yields were generally only 8 to 10 t/ha for both materials. Larger healthy seed tubers produced significantly higher yields at the low and high elevation sites. Tuberlets of 1–5g were also able to give yields of 20 t/ha in the mid-elevation site. These results indicate that cuttings and small tuberlets are two ways of growing potatoes which need to be further evaluated for socioeconomic assessment by farmers. 相似文献