Effect of Zataria multiflora essential oil on Alternaria alternata in vitro and in an assay on tomato fruits

Journal of Plant Diseases and Protection - The present study aimed to evaluate the effects of Zataria multiflora essential oil at different concentrations (0, 100, 200, 300 and 500 ppm) on the...     

Investigation of salinity impacts on germination and growth of two forest tree species at seedling stage

Soil salinity is becoming an increasingly serious constraint to plant growth in many parts of the world;this is particularly common in semi-arid and arid zones. This study was conducted to evaluate the...     

Low developmental competence and high tolerance to thermal stress of ovine oocytes in the warm compared with the cold season

Tropical Animal Health and Production - Heat stress can potentially affect most aspects of reproduction in mammals. To our knowledge, no studies have ever been conducted for evaluating the...     

Optimizing ex situ genetic resource collections for European livestock conservation

Ex situ collections offer the potential to reduce extinction risks, affording option to society in maintaining future breeding opportunities for productivity and heritage traits. However, how much should we be seeking to collect and conserve in gene banks, and where? We developed a mathematical model to optimize logistical decisions of breed conservation choices and to evaluate alternative scenarios for efficiently re‐allocating genetic materials currently stored in different European gene banks, allowing for cross‐country collections, cost and cryogenic capacity differentials. We show how alternative allocations for the breeds that are currently stored in 11 European gene banks could reduce overall conservation costs by around 20% by selecting cryogenic banks that have relatively lower combination of fixed and collection costs, and are geographically closer to collection regions. Our results show that centralizing collections in one gene bank would double the costs, relative to collective European collections approaches. We also calculate marginal costs of collections and show that increasing diversity within the gene banks implies in higher costs per conserved breed.     

Effects of irrigation strategies and soils on field grown potatoes: Yield and water productivity

Yield and water productivity of potatoes grown in 4.32 m² lysimeters were measured in coarse sand, loamy sand, and sandy loam and imposed to full (FI), deficit (DI), and partial root-zone drying (PRD) irrigation strategies. PRD and DI as water-saving irrigation treatments received 65% of FI after tuber bulking and lasted for 6 weeks until final harvest. Analysis across the soil textures showed that fresh yields were not significant between the irrigation treatments. However, the same analysis across the irrigation treatments revealed that the effect of soil texture was significant on the fresh yield and loamy sand produced significantly higher fresh yield than the other two soils, probably because of higher leaf area index, higher photosynthesis rates, and “stay-green” effect late in the growing season. More analysis showed that there was a significant interaction between the irrigation treatments and soil textures that the highest fresh yield was obtained under FI in loamy sand. Furthermore, analysis across the soil textures showed that water productivities, WP (kg ha⁻¹ fresh tuber yield mm⁻¹ ET) were not significantly different between the irrigation treatments. However, across the irrigation treatments, the soil textures were significantly different. This showed that the interaction between irrigation treatments and soil textures was significant that the highest significant WP was obtained under DI in sandy loam. While PRD and DI treatments increased WP by, respectively, 11 and 5% in coarse sand and 28 and 36% in sandy loam relative to FI, they decreased WP in loamy sand by 15 and 13%. The reduced WP in loamy sand was due to nearly 28% fresh tuber yield loss in PRD and DI relative to FI even though ET was reduced by 9 and 11% in these irrigation treatments. This study showed that different soils will affect water-saving irrigation strategies that are worth knowing for suitable agricultural water management. So, under non-limited water resources conditions, loamy sand produces the highest yield under full irrigation but water-saving irrigations (PRD and DI) are not recommended due to considerable loss (28%) in yield. However, under restricted water resources, it is recommended to apply water-saving irrigations in sandy loam and coarse sand to achieve the highest water productivity.     

Effect of apple peel extract as natural antioxidant on lipid and protein oxidation of rainbow trout (Oncorhynchus mykiss) mince

International Aquatic Research - The aim of this study was to use apple peel extract (APE) as antioxidant for inhibition of lipid and protein oxidation in rainbow trout (Oncorhynchus mykiss) mince...     

Utilization of Site-Specific Recombination in Biopharmaceutical Production

Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and process development, expression level is unpredictable and unstable because of the random location of integration in the genome. Site-specific recombination techniques are capable of producing stable and high producer clonal cells; therefore, they are gaining more importance in the biopharmaceutical production. Site-specific recombination methods increase the recombinant protein production by specifically inserting a vector at a locus with specific expression trait. The present review focused on the latest developments in site-specific recombination techniques, their specific features and comparisons.     

High performance fibers production process using Taguchi experimental design

In this research, results of an experimental interaction effect of operating parameters on tensile strength carbon fibers from a commercial PAN-based precursor are investigated. Ten parameters at two and four levels (L₃₂=2¹×4⁹) were investigated: stabilization temperature at first stage (STFIS), stabilization duration time at first stage (SDTFIS), stabilization temperature at second stage (STSS), stabilization duration time at second stage (SDTSS), stabilization temperature at third stage (STTS), stabilization duration time at third stage (SDTTS), stabilization temperature at fourth stage (STFOS), stabilization duration time at fourth stage (SDTFOS), carbonization temperature (CT), and carbonization duration time (CDT). In this study, Taguchi method was used initially to plan a minimum number of experiments. Statistical analysis, analysis of variance (ANOVA), was also employed to determine the relationship between experimental conditions and yield levels. ANOVA was applied to calculate sum of square, variance, ratio of factor variance to error variance and contribution percentage of each factor on response. The results show that increasing all of parameters improves tensile strength performance. The optimum levels of influential factors, determined for tensile strength are STFIS 200 °C, SDTFIS 120 min, STSS 225 °C, SDTSS 120 min, STTS 240 °C, SDTTS 120 min, STFOS 260 °C, SDTFOS 60 min, CT 1400 °C and CDT 10 min. The results showed that CT and ODTFIS are the most and the less effective factors on response, respectively.     

Modulatory function of NMDA glutamate receptor on MC<Subscript>3</Subscript>/MC<Subscript>4</Subscript> receptors agonist-induced hypophagia in neonatal meat-type chicken

Melanocortin 3 and 4 receptors (MC₃R and MC₄R) are known as the main receptors for melanocortin-induced hypophagia in mammalian and poultry. Also, central glutamatergic system has mediatory role on function of the melanocortin system in some brain areas. So, the aim of the current study was to determine the role of MC₃/MC₄ receptors agonist on food intake and its interaction with glutamatergic in 3-h food-deprived (FD₃) neonatal broilers. In experiment 1, chickens were intracerebroventricular (ICV) injected with control solution, MTII (MC₃/MC₄ receptors agonist; 2.45, 4.8 and 9.8 pmol). In experiment 2, control solution, SHU9119 (MC₃/MC₄ receptors antagonist; 0.5, 1 and 2 nmol) were ICV injected. In experiment 3, birds ICV injected with control solution, SHU9119 (0.5 nmol), MTII (9.8 pmol) and co-injection of the SHU9119 + MTII. Experiments 4–8 were similar to experiment 3, except birds injected with MK-801 (NMDA glutamate receptors antagonist, 15 nmol), CNQX (AMPA glutamate receptors antagonist; 390 nmol), AIDA (mGLUR₁ glutamate receptors antagonist; 2 nmol), LY341495 (mGLUR₂ glutamate receptors antagonist; 150 nmol) and UBP1112 (mGLUR₃ glutamate receptors antagonist; 2 nmol) instead of SHU9119. Then, cumulative food intake was recorded until 120 min after injection. According to the results, dose dependent hypophagia observed after ICV injection of the MTII (p < 0.05). ICV injection of SHU9119 significantly increased food intake in birds (p < 0.05). Co-injection of SHU9119 + MTII significantly inhibited MTII- induced hypophagia in neonatal chicks (p < 0.05). In addition, hypophagia- induced by MTII was significantly attenuated with co-injection of MTII + MK-801(p < 0.05). These results suggested MC₃ and MC₄ receptors have inhibitory role on food intake and this effect is probably mediated by NMDA glutamate receptors in neonatal chickens.     

Late defoliation and wheat yield: Little evidence of post-anthesis source limitation

The effect of source reduction on yield and protein content of bread wheat under well-watered and mild drought stress condition in a semi-arid climate was studied. Field experiments were conducted at the Tehran University research farm during 2003–2004 and 2004–2005 growing seasons. Mild drought stress was imposed when plants were at the second node stage by repeatedly withholding watering and re-irrigating when they showed symptoms of wilting or leaf rolling. Partial defoliations (all leaf lamina other than flag leaves were removed) were imposed at booting and anthesis; complete defoliation was imposed at anthesis (defoliation treatments were applied to all plants of each plot). Drought stress caused a significant increase in the remobilization of pre-anthesis reserves to the grain. Defoliation did not significantly affect remobilization. Grain yield and 1000-grain weight was reduced slightly by drought stress, but in most cases it was not significantly reduced by defoliation. Significant changes were not observed for grain protein content between defoliated and control plots. The results suggested that grain yield of the cultivar used under the condition tested is more controlled by sink than source strength.