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31.
Establishment of reliable methods of in vitro pollen germination and pollen preservation of Brassica rapa (syn. B. campestris) 总被引:4,自引:0,他引:4
A simple and reliable method for evaluating the viability of Brassica pollen was established in which the in vitro germination
rate of pollen was adopted as the index of the viability of pollen grains. Pollen grains were preincubated in an atmosphere
in which the relative humidity (RH) was fixed to 52% or 66% at 20 °C for 5 hours. They were cultured for 16 hours at 25 °C
in a liquid Kwack's medium (1964) supplemented with 20% sucrose, and the pH was adjusted to 8.0. They were then observed under
a microscope and the number of germinating and unchanged pollen grains were counted. The germination rate of pollen was improved
and stabilized by preincubation and the use of a high pH medium. More than 90% of the freshly harvested pollen grains of Brassica
rapa (syn. B. campestris) germinated constantly in these conditions Undehisced anthers were collected from flowers at anthesis
and dehydrated by incubation at 20 °C for 16–24 hours in an atmosphere where the RH was fixed to 15% or 32%. They were put
into a plastic vial and preserved in a freezer at -20 °C. The germination percentage of the preserved pollen was scored at
intervals during preservation. The germination rate of the pollen grains preserved at -20°C for 1 year was higher than 50%
and the pollen proved to be efficient for seed set. Most of the seeds germinated normally.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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35.
Yoko Fujii Takuma Aoki Hiroshi Takano Ryokichi Ishikawa Yoshito Wakao 《Journal of Veterinary Cardiology》2009,11(2):147-151
Three dogs presented for the evaluation of cardiac murmurs were diagnosed with aberrant arteriovenous shunts. All cases demonstrated the following findings: 1) relatively soft continuous murmur loudest at the left heart base resembling patent ductus arteriosus (PDA); 2) shunt flow signals in the pulmonary artery on echocardiography; and 3) no PDA on selective angiography, but evidence of anomalous shunting vessels from thoracic aorta to pulmonary vasculature. An aberrant arteriovenous shunt should be considered when a continuous murmur of relatively small intensity is heard. 相似文献
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37.
Tada Y Yano N Takahashi H Yuzawa K Ando H Kubo Y Nagasawa A Chin K Kawamata Y Sakai R Ohashi N Ogata A Nakae D 《Journal of toxicologic pathology》2010,23(1):39-47
A subchronic feeding study of l-serine (l-Ser) was conducted with groups of 10 male and 10 female Fischer 344 rats fed a powder diet containing 0, 0.06, 0.5, 1.5 or 5.0% concentrations of l-Ser for 90 days. There were no toxicologically significant, treatment-related changes with regards to body weight, food intake, water intake or urinalysis data. In several of the hematology, serum biochemistry and organ weight parameters, significant changes were observed between some of the treated groups and the controls. All these changes, however, were subtle and lacked any corresponding pathological findings. In addition, the increased or decreased values remained within the range of the historical control values. In fact, histopathological assessment revealed only sporadic and/or spontaneous lesions. In conclusion, the no-observed-adverse-effect-level (NOAEL) for l-Ser was, therefore, determined to be at least a dietary dose of 5.0% (2765.0 mg/kg body weight/day for males and 2905.1 mg/kg body weight/day for females) under the present experimental conditions. 相似文献
38.
Osawa T Miura M Yamagishi N Kim D Fukuda N Fukuoka H Sasaki J Goryo M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1253-1255
A six-month-old Japanese Black bull was found to have no left testis in the scrotum. A fist-sized mass was palpated per rectum. Two months later, hCG was injected and blood samples were collected before and after injection. No testosterone response to hCG was observed. On the cut surface of the excised mass, most of the mass was composed of homogeneous adipose-like tissue. The rest of the surface was composed of a well-circumscribed testicular parenchyma-like tissue (18 × 16 × 15 mm). Histology revealed diffusely distributed mature adipocytes and septa of fibrous connective tissue. Neither germ cells nor spermatozoa were observed in the seminiferous tubules. A diagnosis was made of fibrolipoma and aspermatogenesis of the left cryptorchid testis. 相似文献
39.
Doi Y Tamano S Kawabe M Sano M Imai N Nakashima H Furukawa F Hagiwara A Otsuka M Shirai T 《Journal of toxicologic pathology》2011,24(4):207-213
This study was conducted to determine the concordance of results for a pair of structural
isomers, 2-nitropropane (2-NP) and 1-nitropropane (1-NP), using the rat medium-term liver
carcinogenesis bioassay (Ito test) and previously published long-term carcinogenicity
tests. Male F344 rats were given a single intraperitoneal injection of DEN (200 mg/kg
b.w.) to initiate hepatocarcinogenesis. After 2 weeks, they received per os 0, 0.8, 4 or
20 mg/kg/day of 2-NP or 1-NP six times a week and were subjected to two-thirds partial
hepatectomy at week 3. Non-initiated groups receiving 0 or 20 mg/kg/day were also
included. The animals were sacrificed for quantitative analysis of GST-P-positive foci at
week 8. With the highest dose of 2-NP, significantly increased numbers and areas of
GST-P-positive foci were demonstrated as compared with the respective control but were not
noted with 1-NP. In the non-DEN-initiated groups, many small GST-P-positive foci of less
than 0.2 mm in diameter were also induced in the rats treated with 2-NP at 20 mg/kg/day
but were lacking with 1-NP. These results strongly support that 2-NP is a complete
hepatocarcinogen with a potent initiation activity, whereas 1-NP is not. 相似文献
40.
Devkota B Takahashi K Matsuzaki S Matsui M Miyamoto A Yamagishi N Osawa T Hashizume T Izaike Y Miyake Y 《The Journal of reproduction and development》2011,57(3):373-378
The present study investigated the basal levels and GnRH-induced responses of peripheral testosterone and estrogen in Holstein bulls with poor semen quality. On the basis of semen parameters, bulls (n=5) having poor semen quality were selected as experimental bulls, and good semen quality bulls (n=4) were used as control bulls. Both groups were treated intramuscularly once with GnRH (250 μg of fertirelin acetate). Blood samples were collected at -1 day (d), -30 min and 0 h (treatment) followed by every 30 min for 5 h and 1, 3 and 5 d post-GnRH treatment (PGT), and LH, testosterone and estradiol-17β (E(2)) concentrations were measured. The pretreatment concentrations were used as basal levels. The percentage increments based on the 0-h levels were calculated per bull for each sampling time until 5 h PGT, and differences were compared between the experimental and control groups. The PGT concentrations of testosterone and basal and PGT concentrations of E(2) were significantly lower in the experimental group. The testosterone increment in the experimental group was delayed and significantly lower from 1 to 5 h PGT than those in the control group. It can be suggested that bulls with poor semen quality have delayed and lower GnRH-induced testosterone response and may also have lower estrogen levels. 相似文献