Ascorbic Acid Enhances Growth and Yield of Sweet Peppers (Capsicum annum) by Mitigating Salinity Stress

Salinity is a crucial problem which has affected crop productivity globally. Ascorbic acid is considered helpful against abiotic stresses due to its powerful antioxidant potential. In the pot experiment, salinity stress (0, 35, 70, and 105?mM) was applied to sweet peppers in split doses after 20 days of transplantation. To mitigate the adverse effects of salinity, ascorbic acid (0, 0.40, 0.80, and 1.20?mM) was applied as foliar spray after a 6-day interval during vegetative growth. Sweet pepper plants sprayed with distilled water (control) recorded maximum plant height (cm), leaf area (cm²), number of branches, stem diameter (mm), number of fruit plant^?1, fruit diameter (cm), yield plant^?1 (g), and chlorophyll content (mg 100?g^?1), while the maximum polyphenol oxidase (PPO) activity (unit mg protein^?1 min^?1) and ascorbate peroxidase (APX) activity (unit mg protein^?1 min^?1) were recorded in plants treated with 70?mM NaCl application. Salinity stress beyond 70?mM significantly reduced all the studied parameters. An ascorbic acid concentration of 1.20?mM significantly mitigated the negative effects of salt stress and recorded maximum plant height (cm), number of leaves plant^?1, leaf area (cm²), number of branches plant^?1, stem diameter (mm), number of fruit plant^?1, fruit diameter (cm), yield plant^?1 (g), chlorophyll content (mg 100?g^?1), PPO activity (unit mg protein^?1 min^?1), and APX activity (unit mg protein^?1 min^?1). Hence, a 1.20?mM concentration of foliar ascorbic acid could be used in saline conditions up to 70?mM of sodium chloride (NaCl) for better growth, productivity, and enzymatic activity of sweet peppers.

     

Efficacy of various concentrations of synthetic hormones on the induced breeding of Channa marulius (Sole)

A study was conducted to determine the artificial breeding response, embryonic and larval development of giant snakehead, Channa marulius, with the application of various dosages of stimulatory hormones: Suprefact® (LHRH) agonist and Ovaprim® (GnRH + dopamine inhibitor). First and second hormonal dosages of Suprefact® (diluted) and Ovaprim® (0.3, 0.4, and 0.5 ml for male and 0.8, 0.9, and 1.0 ml for females per kilogram body weight) were used. There were three treatments T₁, T₂, and T₃ and each treatment had three replicates. Male fish were treated with T₁ (0.3), T₂ (0.4), T₃ (0.5) ml kg⁻¹ of body weight while female fish with T₁ (0.8), T₂ (0.9), T₃ (1.0) ml kg⁻¹ of body weight. The results showed that fish stimulated with T₃ (1.0) obtained better fecundity rate (2951.7) followed by T₂ (0.4) (2678.3), while the lowest fecundity (466.7) was recorded in T₁. The numerically higher values of gonado-somatic index (males and females) and fertilization rate appeared in T₂. The highest survival rate (97.1%) was found in T₃ followed by T₂ (97.0%), while the lowest (32.2%) in T₁. In conclusion, the application of Suprefact® and Ovaprim® at 0.4 and 0.5 ml kg⁻¹ body weight for male and 0.9 and 1.0 ml kg⁻¹ for female fish successfully stimulated snakehead fish induced breeding.     

In ovo oral vaccination with cmpylobacter jejuni establishes early development of intestinal immunity in chickens

1. Chick embryos were orally immunised at day 16 of incubation by injection of heat‐killed Campylobacter jejuni organisms into the amniotic fluid. The response to vaccination was observed at 5 d after hatching or, in some birds which received a postnatal oral booster vaccination, at 7 d after hatching, and the response was observed at 14 d of age.

2. The titres of antibody in serum, bile and intestinal scrapings, the distribution of immunoglobulin‐containing cells in the spleen, duodenum and ileum and the expression on peripheral blood leukocytes (PBL) of the T cell surface markers CD3, CD4 and CD8 were determined.

3. Whereas low titres of anti‐flagellin antibody were detected in serum, bile and intestinal scrapings of unimmunised birds, high titres were observed in immunised birds.

4. An increase in antibody of all isotypes was detectable in serum but the elevation in IgA antibody in intestinal scrapings and bile was particularly striking. This response was reflected in a dramatic increase in immunoglobulin‐containing cells, detected by fluorescent histology, particularly diose associated with IgA and IgM isotypes in the spleen and intestine of immunised birds.

5. Secondary oral boosting after hatching resulted in a depression in serum anti‐flagellin antibody in immunised birds compared to pre‐boosting titres (although still significandy higher than in non‐immunised controls) but an increase in IgA antibody in intestinal scrapings and bile. The number of immunoglobulin‐containing cells was also increased after boosting.

6. Neither immunisation regimen caused a significant change in the numbers of circulating CD3, CD4 or CD8 T cells.

7. These results indicate that in ovo oral immunisation with C. jejuni antigens stimulates the precocious development of immunity in chicks.     

Banteng and Bali cattle in Indonesia: status and forecasts

Bali cattle still represents 27% of the total cattle population in Indonesia, and it is considered the pillar breed for small farmers. Moreover, it is a breed of evolutionary importance regarding its direct ancestry from Banteng. However, there is a need for the establishment of a rational system for the evaluation of breeding soundness for indigenous Bali bulls to be used as sires for artificial insemination breeding programmes. Moreover, there is a need for cryobanking of well-identified genetic resources pertaining their use in evolutionary research and application as essential germplasm in breeding programmes.     

Mycelium of arbuscular mycorrhizal fungi increases soil water repellency and is sufficient to maintain water-stable soil aggregates

Using an in vitro bioreactor system in which the arbuscular mycorrhizal (AM) fungus Glomus intraradices was grown in a soil devoid of detectable living microbes, we could show that the mycelium of this fungus contributed to the maintenance of water-stable soil aggregates and increased soil water repellency, as measured by water drop penetration time. This is to our knowledge the first demonstration of a causal link between AM fungal growth and water repellency of soil aggregates. Our results also place AM fungal contributions to soil aggregation on a firm mechanistic footing by showing that hyphae are sufficient to produce effects, in the absence of other soil biota, which have always been included in previous studies.     

Green nano-emulsion intervention for water-soluble glyphosate isopropylamine (IPA) formulations in controlling Eleusine indica (E. indica)

This article describes the development of environmentally friendly nano-emulsion system for water-soluble herbicide application. Pseudoternary phase diagrams were established in the emulsion system of fatty acid methyl esters (FAMEs)/alkylpolyglucosides (APG) and/or 3-(3-hydroxypropyl)-heptamethyltrisiloxane (organosilicone)/water encompassed with 41% (w/w) glyphosate isopropylamine (IPA) as herbicide active. Pre-formulations were selected from isotropic (L) region in the phase diagrams and their emulsion system characteristics were determined. The microemulsion systems were chosen and then dispersed into water using low-energy stirring method (200 rpm for 5 min). Oil-in-water (O/W) nano-emulsions were formed with particle sizes of diameter less than 200 nm. The nano-emulsion systems showed significantly lower surface tension than a commercial formulation (Roundup®). In the biological application study, treatments of nano-emulsion formulations and Roundup® were applied on narrow-leaved weed Eleusine indica. Multiple doses of glyphosate IPA of the treatments were applied for the construction of dose-response curves for determination of effective dose (ED₅₀). The nano-emulsion formulation showed lower ED₅₀ was 0.40 kg a.e./ha in controlling the weed than Roundup® was 0.48 kg a.e./ha. This finding suggested that the possibility of using nano-emulsion system to increase penetration and uptake of glyphosate IPA.     

Genotypic, phenotypic, biochemical, physiological and pathogenicity-based categorisation of Acanthamoeba strains

The genus Acanthamoeba includes more than 20 morphological species, but classification is problematical. Recently, the discovery of substantial interstrain differences in ribosomal DNA (rDNA) sequences has prompted questions about the relatedness of strains of the same species. In this study, therefore, we have investigated relationships between two isolates of A. polyphaga, CCAP 1501/3c and ATCC 30871, using morphological, biochemical, physiological, molecular and cytotoxicity assays. We observed that A. polyphaga ATCC 30871 exhibited up to six arms in endocyst while A. polyphaga CCAP 1501/3c exhibited a maximum of 5 arms thus indicating their position in group 2 and 3, respectively. Acanthamoeba polyphaga ATCC 30871 exhibited growth at 37 degrees C and growth on 1M mannitol plates while A. polyphaga CCAP 1501/3c did not. In addition, both isolates exhibited differences in isoenzyme banding patterns and rDNA restriction fragment polymorphisms. More importantly, A. polyphaga ATCC 30871 produced cytotoxicity on corneal epithelial cells while A. polyphaga CCAP 1501/3c had no effects, suggesting differences in pathogenicity. Thus, all the results provide evidence for significant differences between the strains and further provided the basis for reclassification of the isolates. Implications of these results in the clinical diagnosis of pathogenic Acanthamoeba are discussed.     

Effects of strains, strain crosses and environments on additive genetic and phenotypic variances in Drosophila melanogaster

SUMMARY: The stability of phenotypic, additive genetic and environmental variances of thorax length of Drosophila melanogaster in pure and synthetic strains was examined in two different environments. Two pure strains from different geographic locations (Melbourne and Townsville) were used, together with three synthetic populations formed from them. The existence of differences in thorax length between the Melbourne and Townsville populations, genotype by environment interaction, and heterosis in crosses between these populations indicate that they are genetically different. Thus geographic separation can cause differences in mean thorax length of flies from different populations. Both the difference in selection histories between the two localities and drift could lead to these differences. Up to the thirty fifth generation there was no evidence of any reduction in the difference between the Melbourne and Townsville populations, in either laboratory environment. The genetic differentiation of strains therefore may be maintained over many generations under new environmental conditions. The fluctuation over generations of heterosis of thorax length is possibly caused by the fluctuation of the rate of loss of favourable epistatic interaction in crossbred genotypes in combination with natural selection effects. V(p) was significantly higher in poor than in the good environment. This higher V(p) in the poor environment is most likly due to higher non additive genetic variance. V(p) was also significantly influenced by strain. In general, V(p) values of synthetic strains were higher than those of pure strains in both environments. Finally, the additive and environmental variances of thorax length were relatively stable across strains, generations and environments. ZUSAMMENFASSUNG: Wirkung von Herkünften, Kreuzungen und Umwelten auf additiv-genetische und ph?notypische Varianzen in Drosophila melanogaster Die Stabilit?t ph?notypischer, additiv-genetischer und umweltbedingter Varianzen der Thoraxl?nge von Drosophila melanogaster in reinen und synthetischen Herkünften wurde in zwei verschiedenen Umwelten überprüft. Zwei reine Herkünfte von verschiedenen Gegenden (Melboune und Townsville) wurden zusammen mit drei zwischen ihnen gebildeten synthetischen Populationen untersucht. Unterschiede in Thoraxl?nge zwischen Melbourne- und Townsvilleherkünften, Genotypumweltinteraktionen und Heterosis in Kreuzungen zwischen diesen Populationen zeigen, da? sie sich genetisch unterscheiden. Die geographische Trennung kann also Unterschiede in der mittleren Thoraxl?nge zur Folge haben, wobei unterschiedliche Selektionsgeschichte in beiden Gegenden und Drift dies verursachen k?nnen. Bis zur 35. Generation gab es in keinem Labormilieu einen Hinweis auf eine Reduktion der Unterschiede zwischen den beiden Populationen. Die genetische Differenz der Herkünfte erh?lt sich daher auch unter neuen Umweltverh?ltnissen über viele Generationen. Die Schwankung in Heterosis für Thoraxl?nge ist m?glicherweise durch Schwankungen in der Verlustrate günstiger epistatischer Interaktionswirkungen in Kreuzungsgenotypen zusammen mit natürlichen Selektionswirkungen verursacht. V(p) war durch Umweltbedingungen signifikant beeinflu?t und h?her in schlechtem als in gutem Milieu. Der hohe Wert in schlechtem Milieu ist wahrscheinlich auf nicht-additiv-genetische Varianz zurückzuführen. V(p) wurde auch signifikant durch Herkunft beeinflu?t und Werte in synthetischen Linien waren h?her Linien in beiden Milieus. Additive und umweltbedingte Varianzen waren über Linie, Generationen und Umwelt relativ stabil.