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101.
Kang-Seuk Choi Eun-Kyoung Lee Woo-Jin Jeon Jun-Hun Kwon 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(3):205-211
Newcastle disease (ND) caused by virulent Newcastle disease virus (NDV) is a highly contagious viral disease of poultry. Virulent NDVs characteristically have a multibasic amino acid sequence (virulence motif) such as 112RRQKRF117 at the cleavage site of the precusor fusion (F0) protein. The antigenic and immunogenic characteristics of the virulence motif 112RRQKRF117 in the F0 protein of virulent NDVs were investigated. Epitope mapping analysis revealed that a RRQKRF-specific monoclonal antibody 4G2 recognized the KRF section of the motif. A synthetic peptide bearing the RRQKRF motif reacted strongly with sera from virulent NDV (with RRQKRF motif)-infected chickens. These sera also showed reactivity to peptides bearing other virulence motifs (112KRQKRF117, 112RRQRRF117 and 112RRRKRF117) but not an avirulence motif (112GRQGRL117) by ELISA. The synthetic bearing RRQKRF motif reacted with 60% to 91% of sera taken from surviving chickens on ND outbreak farms but not with sera from vaccinated birds, even though most of the sera had antibody to NDV due to vaccination. This indicates that the virulence motif has the potential to differentiate virulent NDV infected birds from vaccinated birds. 相似文献
102.
Effect of hydrogen peroxide exposures on mucous cells and lysozymes of gill tissues of olive flounder Paralichthys olivaeceus
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We investigated the effects of hydrogen peroxide on mucous cells and lysozymes in gill tissue of olive flounder. Morphological analysis revealed significant changes in the number of mucous cells after exposure to 300 and 500 mg L ? 1 hydrogen peroxide; however, there was no detectable difference in the 100 mg L ? 1 group. Lysozyme activities declined gradually in the gill mucus, but in contrast activities in serum increased significantly following the completion of treatment. The hepatic c‐ and g‐type lysozyme levels showed significant responses, indicating that the liver is a major lysozyme producer in response to stress condition; however, in the head kidney, there were no significant changes in the g‐type lysozyme level in all the groups, while the only detectable difference with the c‐type gene in the 300 mg L ? 1. While the treatments showed no significant changes compared with the control for either branchial lysozyme, there were significant differences in c‐type lysozyme between the 3rd treatment and 5 day. Taken together, our data indicate that hydrogen peroxide treatment may lead to modulation of the innate immune response of olive flounder; therefore, the application of hydrogen peroxide in a field system will require more attention to dose and timing effects. 相似文献
103.
Hong SY Kim JY Ahn HY Shin JH Kwon O 《Journal of agricultural and food chemistry》2012,60(12):3086-3091
Nitric oxide (NO) produced by endothelial nitric oxide synthase (eNOS) is a fundamental regulator of systemic blood pressure. Ginsenosides from Panax ginseng have been investigated in vitro for the molecular and biochemical mechanisms by which they stimulate NO release in vascular endothelial cells; however, little research has been done to confirm the physiological relevance of these in vitro studies. To address this research gap, the effects of a P. ginseng extract rich in ginsenosides from protopanaxatriol on spontaneously hypertensive rats (SHRs) was examined. Ginseng extract administration stimulated nongenomic Akt-mediated eNOS activation, enhanced NO production, improved vessel wall thickening, and alleviated hypertension in SHRs, confirming the physiological relevance of previous in vitro studies with ginsenosides. 相似文献
104.
105.
106.
Joonseok Koh Sang-Mo Kang Soo-Jin Kim Min-Kyung Cha Yoon-Jung Kwon 《Fibers and Polymers》2006,7(2):180-185
A pineapple protease, bromelain, was used to improve the dyeing properties of protein fibers such as wool and silk. The optimal
condition for the activity of the pineapple protease was about 60 °C at pH 7. The wool and silk were treated with the protease
extracted from a pineapple and the K/S values of the dyed wool and silk were measured using a spectrophotometer in order to
compare the dye uptake. The protease treatment enhanced the dyeing properties of protein fibers without severe changes in
mechanical properties. The surface appearances of protease-treated fibers were observed by microscopy. 相似文献
107.
Youn?Hee?Jeon Won?Young?Jeong Jung?Woo?Park Seung?Kook?AnEmail author 《Fibers and Polymers》2003,4(4):151-155
The abrasion behavior of three kinds of warp knitted fabrics, which are normally used for upper sole of footwear, was evaluated.
We measured the changes of mechanical and structural properties of each sample as abrasion cycle increased. Each sample showed
similar trends in compression and surface properties but there were significant differences in abrasion rate among the samples.
The mechanical properties showed remarkable differences with directions. The frictional coefficient (MIU) of fabric surface
increased at the beginning of abrasion and decreased as abrasion cycles increased. The weight and thickness of the fabric
linearly decreased with abrasion cycles. The surface roughness (SMD) and the compressional resilience (RC) decreased as abrasion
cycles increased while compressional energy (WC) increased. 相似文献
108.
The self-assembly of asymmetric ABC triblock copolymers in the ordered structure is investigated using an isothermal-isobaric
molecular dynamics simulation. Unlike symmetric ABC triblock copolymers, more fascinating morphologies are observed in asymmetric
ones because of a larger difference of incompatibility between the components. Various modes of self-assembly in assymmetric
ABC triblock copolymers are also observed depending on the block composition. When the composition of block A is changed from
0.125 to 0.25 at the samef
B
=0.25, the morphological transition from the “cylinder in cylinder” to “cylinders at cylinder” structure is observed in the
simulation. In the case of ABC triblocks withf
B
=0.5, a lamellar-type structure is changed to a cylinder-type structure with increasing the length of block A. When the mid-block
length increases further tof
B
=0.625, the “spheres on cylinder” structure is observed in both the A10B50C20 and A20B50C10 triblocks. From these results, the phase diagram of ABC triblock copolymers can be constructed.
Dedicated to Professor Won-Jei Cho on the occasion of his retirement. 相似文献
109.
Brainard BM Epstein KL LoBato D Kwon S Papich MG Moore JN 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2011,25(1):116-122
Background: Critically ill horses are susceptible to thrombotic disease, which might be related to increased platelet reactivity and activation. Objectives: To compare the effect of oral clopidogrel and aspirin (ASA) on equine platelet function. Animals: Six healthy adult horses. Methods: Horses received clopidogrel (2 mg/kg PO q24h) or ASA (5 mg/kg PO q24h) for 5 days in a prospective randomized cross‐over design. Platelet aggregation responses to adenosine diphosphate (ADP) and collagen via optical aggregometry, and platelet secretion of serotonin (5HT) and production of thromboxane B2 (TXB2) by ELISA were evaluated. In horses receiving clopidogrel, high‐performance liquid chromatography analysis for clopidogrel and its carboxylic‐acid metabolite SR 26334 was performed. Results: SR 26334 was identified in all clopidogrel‐treated horses, although the parent compound was not detected. Clopidogrel resulted in decreases in ADP‐induced platelet aggregation persisting for 120 hours after the final dose. ADP‐induced platelet aggregation decreased from a baseline of 70.2 ± 14.7% to a minimum of 15.9 ± 7.7% 24 hours after the final dose (P < .001). Collagen‐induced aggregation decreased from a baseline of 93 ± 9.5% to a minimum of 70.8 ± 16.9% 48 hours after the final dose (P < .001). ASA did not decrease platelet aggregation with either agonist. ASA decreased serum TXB2 from a baseline value of 1310 ± 1045 to 128 ± 64 pg/mL within 24 hours (P < .01). Conclusions and Clinical Importance: Clopidogrel effectively decreases ADP‐induced platelet aggregation in horses, and could have therapeutic applications for equine diseases associated with platelet activation. 相似文献
110.
Hyon MK Kwon E Choi HJ Kang BC 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(6):739-745
There is a need for a new liver fibrosis model of immunodeficient mice to study the effects of cell therapy on liver disease because there are not many animal models available to study the effects of cell therapy. In this study, we induced liver fibrosis using dimethylnitrosamine (DMN) in NOD/SCID mice to create an animal model for liver disease. DMN (5 mg/kg, i.p.) was injected intraperitoneally for three consecutive days per week for 6 or 8 weeks, and the mice were sacrificed at weeks 0, 4 and 8 after the last DMN injection. The 6-week DMN-treated group gradually recovered from serum biochemical changes, histopathological toxic effects and lesions in the liver at weeks 4 and 8 after the last DMN injection. However, the progression of liver fibrosis and toxic levels were maintained in the 8-week DMN-treated group at week 4 after the last DMN injection. The increases in iron and extracellular matrix (collagen) in the DMN-treated group were confirmed by Prussian blue (PB) and Masson's trichrome (MT) staining, respectively. Additionally, activation of hepatic stellate cells was observed by alpha smooth muscle actin (α-SMA) immunostaining and western blot. In conclusion, treatment of NOD/SCID mice with 5 mg/kg of DMN for 8 weeks can be used to induce an appropriate animal model of disease for liver fibrosis. This model may be useful for evaluation of the efficacy and safety of cell therapies such as human mesenchymal stem cell therapy. 相似文献