Identification of the tetracycline resistance gene, tet(M), in Erysipelothrix rhusiopathiae

This is the first report to demonstrate the presence of tet(M) in naturally occurring isolates of tetracycline-resistant Erysipelothrix rbusiopathiae, which causes swine erysipelas. The tet(M) gene was isolated from E. rhusiopathiae strain KY5-42. The nucleotide and the deduced amino acid sequence were 99% identical to the tet(M) gene from Enterococcus faecalis. The gene was necessary and sufficient for the expression of tetracycline resistance in Escherichia coli. The presence of the tet(M) gene in the 114 tetracycline-resistant E. rhusiopathiae isolates from diseased pigs was detected by the polymerase chain reaction assay. The specific amplified DNA fragment was obtained from all 114 tetracycline-resistant strains. It was suggested that the tet(M) gene was widely present in the field isolates of E. rhusiopathiae resistant to tetracycline.     

罗氏沼虾高产高效养殖技术试验

在总面积为８８６７ｍ＾２的３口池塘中进行了罗氏沼虾高产高效养殖试验。全年放养２批虾苗共１１０万尾,平均放苗１２３尾／ｍ＾２;共收获罗氏沼虾７６４１ｋｇ,平均８６１７．５ｋｇ／ｈｍ＾２;共消耗饲料１６５００ｋｇ,饲料系数２．１６;总产值３１１６３０元,平均产值３５１４６２．４５元／ｈｍ＾２,总利润１６５３７３元,平均利润１８６５１０．９０元／ｈｍ＾２,利润率为１１３．０７％。本试验获得高产高效的结果证明了所采取的技术措施是可行的。     

CT AND MRI FEATURES OF CAROTID BODY PARAGANGLIOMAS IN 16 DOGS

Carotid body tumors (paragangliomas) arise from chemoreceptors located at the carotid bifurcation. In imaging studies, this neoplasm may be confused with other neck neoplasms such as thyroid carcinoma. The purpose of this retrospective, cross‐sectional study was to describe computed tomographic (CT) and magnetic resonance imaging (MRI) characteristics of confirmed carotid body tumors in a multi‐institutional sample of dogs. A total of 16 dogs met inclusion criteria (14 examined using CT and two with MRI). The most common reason for imaging was a palpable cervical mass or respiratory signs (i.e., dyspnea or increased respiratory noises). The most commonly affected breed was Boston terrier (n = 5). Dogs were predominantly male castrated (n = 10) and the median age was 9 years [range 3–14.5]. Most tumors appeared as a large mass centered at the carotid bifurcation, with poor margination in six dogs and discrete margins in ten dogs. Masses were iso‐ to hypoattenuating to adjacent muscles in CT images and hyperintense to muscles in T1‐ and T2‐weighted MRI. For both CT and MRI, masses typically showed strong and heterogeneous contrast enhancement. There was invasion into the adjacent structures in 9/16 dogs. In six of these nine dogs, the basilar portion of the skull was affected. The external carotid artery was entrapped in seven dogs. There was invasion into the internal jugular vein in three dogs, and into the external jugular, maxillary, and linguo‐facial veins in one dog. Imaging characteristics helped explain some clinical presentations such as breathing difficulties, Horner's syndrome, head tilt, or facial nerve paralysis.     

Anti‐tumour activity of oncolytic reovirus against canine histiocytic sarcoma cells

Canine histiocytic sarcoma is an aggressive, fatal neoplastic disease with a poor prognosis. Lomustine is generally accepted as the first‐line systemic therapy, although this compound does not provide complete regression. Therefore, research into a novel approach against canine histiocytic sarcoma is needed. However, anti‐tumour effects of oncolytic therapy using reovirus against histiocytic sarcoma are unknown. Here, we showed that reovirus has oncolytic activity in canine histiocytic sarcoma cell lines in vitro and in vivo. We found that reovirus can replicate and induce caspase‐dependent apoptosis in canine histiocytic sarcoma cell lines. A single intra‐tumoural injection of reovirus completely suppressed the growth of subcutaneously grafted tumours in NOD/SCID mice. Additionally, we demonstrated that susceptibility to reovirus‐induced cell death was attributable to the extent of expression of type I interferons induced by reovirus infection in vitro. In conclusion, oncolytic reovirus appears to be an effective treatment option for histiocytic sarcoma, and therefore warrants further investigation in early clinical trials.     

高湿粮食贮藏干燥机设计与试验

在对高湿粮食干燥状态参数和水分结合能变化特征理论分析的基础上,设计了一种高湿粮食贮藏干燥机,从理论和试验两方面证实了干燥系统客观能势的利用效果,图解了高湿粮食在贮藏干燥机内的温度及其含水率状态变化过程。试验结果显示,温度在30℃、相对湿度40%时,利用自然空气可将粮食的温度降至低于环境温度10℃、降水速率在0.76%/h以上。研究结果为粮食干燥工艺系统设计指明了高效节能的途径,为大型粮食集中干燥设施提供了高效节能的预干燥设备。     

宝塔区生态环境特征及其生态建设研究

根据实地考察和资料分析 ,应用区域生态环境规划理论对宝塔区生态环境状况进行了评价 ,划分出不同的生态环境质量等级。同时 ,根据社会、经济和环境指标 ,将宝塔区划分为 3个生态经济区、8个生态经济亚区 ,对各区的生态经济特征进行了评价 ,并提出生态建设方向。结合宝塔区生态示范区建设规划 ,以生态农业和生态环境建设为生态示范区主要建设领域 ,按照区域特点 ,提出了各领域的生态建设模式