Nitrogen isotope fractionation during nitrogen transport from ectomycorrhizal fungi,Suillus granulatus,to the host plant,Pinus densiflora

The ¹⁵N natural abundance in Pinus densiflora Sieb. et Zucco that had been inoculated and not inoculated with ectomycorrhizal fungi (Suillus granulatus (L.:Fr.) O. Kuntze) was compared. The inoculated pine needles showed a lower δ¹⁵N value, while the uninoculated ones showed a higher δ¹⁵N value. Higher δ¹⁵N values in the mycelial mat of the ectomycorrhizal fungi compared to those of the inoculated pine needles were also observed. These facts indicate that nitrogen isotope fractionation occurred during the nitrogen transport from mycorrhizal fungi to the host plants.     

Effect of monosilicic acid on hydrolytic polymerization of Fe(III) and structure hydrolytic products

To analyze the mechanism of retarded hydrolytic polymerization of Fe(III) and crystallization of the hydrolytic products in the presence of H₄SiO₄, we measured the changes in the monomeric Fe(III) concentration as a function of pH, H₄SiO₄ concentration, and aging time, and characterized the hydrolytic reaction products by a combination of methods. The monomeric Fe(III) concentration in solutions with higher H₄SiO₄ concentrations was significantly higher than that in an Si-free solution but the differences gradually narrowed with aging time. Chemical equilibrium calculation showed that the concentration of the FeH₃SiO₄ ²⁺ complex was not high enough to account for the reduced rate of hydrolytic polymerization. Electron microscopy and infrared spectroscopy showed that the hydrolytic products were ferrihydrite or Si-containing ferrihydrite and their particle size decreased with increasing H₄SiO₄ concentration. On the basis of these observations, we concluded that the interfering effect of H₄SiO₄ on the hydrolytic polymerization involved two mechanisms: 1) the coexistence of H₄SiO₄ delayed the kinetics of the hydrolytic polymerization of Fe(III) to a certain extent due to the formation of an FeH₃SiO₄ ²⁺ complex, and 2) H₄SiO₄ retarded the growth of Fe(III) hydroxides by binding onto the active sites of the hydrolytic products.     

Demerium exchange of hydroxyl groups in allophane

The nature of amorphous silicates, allophane,ö The name allophane is used in this paper in a dual sense; the one stands for a group of amorphous or nearly amorphous aluminum silicates associated with minor amounts of other metals, and the other in some restricted sense for amorphous silicates other than imogolite. The latter term, imogolite, is used for convenience to denote a component that shows broad x-ray diffractions at 14 (strong), 7.6 (medium) and 5.6 (weak) Å, gives an endotherm at 430°C, and appears as thread-like particles of diameter 100 to 200 Å in electronmicrographs (21). as a main clay constituent determines many of the physical and chemical properties of volcanic ash soils. Recent developments in the study of allophane together with other amorphous inorganic materials in relation to their nature and occurrence in soils have been reviewed by MITCHELL et al. (12).     

Factors Influencing the Determination of Microbial Biomass-Nitrogen in Submerged Soil

In a previous paper (1), a modification of the chloroform fumigation method (2) was proposed for estimating microbial biomass-nitrogen (N) in submerged soil. It was found that a large part of the living bacteria in the submerged soil was killed within about 12 h of the fumigation and was actively mineralized by subsequent anaerobic incubation.

In this paper, the following factors which influence the estimated amount of microbial biomass-N were examined: a) inoculation to fumigated soil, b) duration of anaerobic incubation, c) storage and sieving pretreatments of soil, and d) effect of fumigation on soil organic matter.     

Comparison of Salmonella enterica serovar Abortusequi isolates of equine origin by pulsed-field gel electrophoresis and fluorescent amplified-fragment length polymorphism fingerprinting

Equine paratyphoid is caused by Salmonella enterica serovar Abortusequi, and manifests mainly as abortion in the mare. We compared S. Abortusequi strains isolated in Japan and other countries using pulsed-field gel electrophoresis (PFGE) and fluorescent amplified-fragment length polymorphism (FAFLP) analysis. PFGE analysis of S. Abortusequi strains gave 21-27 fragments ranging in size from 33 to 602kb. Although two PFGE profiles were observed among the 20 S. Abortusequi isolates in Japan, the restriction fragments originating from the chromosome were common between the two profiles. The similarity index of the two profiles was 90.9%, while those between Japanese and five other S. Abortusequi strains were 29.8-37.5%. On the other hand, FAFLP analysis of S. Abortusequi strains generated 64-67 amplified fragments ranging in size from 100 to 400bp. One polymorphic fragment was observed among the 20 S. Abortusequi isolates in Japan. These data indicate the close relation of this agent in Japan. S. Abortusequi strains sharing a common ancestry might have been conserved in Japan.     

Pulmonary histiocytic sarcoma in a rabbit

An approximately 8‐year‐old male castrated Dutch rabbit was evaluated for a 6‐day history of respiratory signs, which began as sneezing and progressed to tachypnea with anorexia. On physical examination, tachypnea and pale mucous membranes were noted. Thoracic radiographs revealed a soft tissue pulmonary mass, fine‐needle aspirates of which confirmed a neoplasia with malignant features suspicious for a histiocytic sarcoma. The rabbit was discharged and due to a rapidly deteriorating condition, the owner declined chemotherapy with Lomustine and elected euthanasia of the rabbit. The affected lung was submitted for histopathology. Histologic sections of the lung were characterized by clusters of histiocytic cells and multinucleated giant cells with occasional invasion of blood and lymphatic vessels. The histologic diagnosis was histiocytic sarcoma. To the authors' knowledge, this is the first case report of histiocytic sarcoma in a rabbit. Based on the clinical and radiologic findings in this case, histiocytic sarcoma should be included in the list of differentials for rabbits presenting with respiratory signs and evidence of a pulmonary mass.     

Potential predictive biomarkers of obesity in Burmese cats

Australian Burmese cats are predisposed to diabetes mellitus and, compared to other breeds, have delayed triglyceride clearance that may result in subtle changes within cells and tissues that trigger specific alterations in gene expression within peripheral blood leucocytes (PBLs). Expression of genes involved in energy metabolism (glucose-6-phosphate dehydrogenase and malate dehydrogenase), lipogenesis (ATP citrate lyase [ACL], fatty acid synthase [FAS] and sterol regulatory binding protein-1c [SREBP-1c]), and insulin signalling (insulin receptor substrates 1 and 2, and phosphatidylinositol-3 kinase), as well as cholesterol lipoprotein subfraction profiling were carried out on PBLs from lean Burmese cats and compared with similar profiles of age and gender matched lean and obese Australian domestic shorthaired cats (DSHs) in an attempt to identify possible biomarkers for assessing obesity.For the majority of the genes examined, the lean Burmese cats demonstrated similar PBL gene expression patterns as age and gender matched obese Australian DSH cats. Lean Burmese had increased expression of ACL and FAS, but not SREBP-1c, a main upstream regulator of lipid synthesis, suggesting possible aberrations in lipogenesis. Moreover, lean Burmese displayed a 3- to 4-fold increase in the very low density cholesterol fraction percentage, which was double that for obese DSH cats, indicating an increased degree of lipid dysregulation especially in relation to triglycerides. The findings suggest that Burmese cats may have a particular propensity for dysregulation in lipid metabolism.     

Occurrence and distribution of settling and newly settled spotted halibut Verasper variegatus and Japanese flounder Paralichthys olivaceus in shallow nursery grounds around Shimabara Peninsula, western Japan

The occurrence and distribution of settling and settled spotted halibut Verasper variegatus were compared with those of Japanese flounder Paralichthys olivaceus in shallow habitats around Shimabara Peninsula, western Japan, to elucidate the settlement and recruitment characteristics of spotted halibut, a rare pleuronectid flatfish in Japan. Push-net sampling conducted at nine sites in March and April 2003 collected spotted halibut from four nurseries located at eastern areas of Shimabara Peninsula in Ariake Bay; Japanese flounder were sampled from eight nurseries. Comparison of microhabitat distributions in a tidal-flat nursery during February?CApril in 2003?C2007 revealed that metamorphosing larvae and juveniles of spotted halibut, which showed the drastic body coloration (black to brown) and behavioral changes (frequent swimming to settlement), were distributed mainly in the lower edge of the intertidal area, whereas Japanese flounder inhabited both intertidal and subtidal areas. Metamorphosing larvae of spotted halibut (12.6?C16.5?mm standard length; n?=?24) were collected in March when the highest densities were observed, whereas metamorphosing larvae of Japanese flounder (10.0?C15.9?mm, n?=?55) were observed during March?CApril. Our results suggest that spotted halibut has a narrower time/space window for recruitment to the specific nurseries compared with Japanese flounder.     

Glucosinolate metabolism,functionality and breeding for the improvement of Brassicaceae vegetables

Unique secondary metabolites, glucosinolates (S-glucopyranosyl thiohydroximates), are naturally occurring S-linked glucosides found mainly in Brassicaceae plants. They are enzymatically hydrolyzed to produce sulfate ions, D-glucose, and characteristic degradation products such as isothiocyanates. The functions of glucosinolates in the plants remain unclear, but isothiocyanates possessing a pungent or irritating taste and odor might be associated with plant defense from microbes. Isothiocyanates have been studied extensively in experimental in vitro and in vivo carcinogenesis models for their cancer chemopreventive properties. The beneficial isothiocyanates, glucosinolates that are functional for supporting human health, have received attention from many scientists studying plant breeding, plant physiology, plant genetics, and food functionality. This review presents a summary of recent topics related with glucosinolates in the Brassica family, along with a summary of the chemicals, metabolism, and genes of glucosinolates in Brassicaceae. The bioavailabilities of isothiocyanates from certain functional glucosinolates and the importance of breeding will be described with emphasis on glucosinolates.