全文获取类型
收费全文 | 132555篇 |
免费 | 7272篇 |
国内免费 | 93篇 |
专业分类
林业 | 5381篇 |
农学 | 4276篇 |
基础科学 | 831篇 |
17047篇 | |
综合类 | 21484篇 |
农作物 | 5399篇 |
水产渔业 | 6702篇 |
畜牧兽医 | 68011篇 |
园艺 | 1802篇 |
植物保护 | 8987篇 |
出版年
2020年 | 1179篇 |
2019年 | 1376篇 |
2018年 | 2034篇 |
2017年 | 2333篇 |
2016年 | 2096篇 |
2015年 | 1744篇 |
2014年 | 2229篇 |
2013年 | 5297篇 |
2012年 | 4119篇 |
2011年 | 5101篇 |
2010年 | 3247篇 |
2009年 | 3392篇 |
2008年 | 4978篇 |
2007年 | 4674篇 |
2006年 | 4359篇 |
2005年 | 4089篇 |
2004年 | 3850篇 |
2003年 | 3980篇 |
2002年 | 3549篇 |
2001年 | 4152篇 |
2000年 | 4255篇 |
1999年 | 3373篇 |
1998年 | 1474篇 |
1997年 | 1388篇 |
1996年 | 1288篇 |
1995年 | 1529篇 |
1994年 | 1360篇 |
1993年 | 1232篇 |
1992年 | 2584篇 |
1991年 | 2700篇 |
1990年 | 2591篇 |
1989年 | 2611篇 |
1988年 | 2369篇 |
1987年 | 2349篇 |
1986年 | 2428篇 |
1985年 | 2341篇 |
1984年 | 1883篇 |
1983年 | 1677篇 |
1982年 | 1149篇 |
1979年 | 1748篇 |
1978年 | 1397篇 |
1977年 | 1151篇 |
1976年 | 1212篇 |
1975年 | 1293篇 |
1974年 | 1500篇 |
1973年 | 1514篇 |
1972年 | 1405篇 |
1971年 | 1304篇 |
1970年 | 1251篇 |
1969年 | 1283篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
961.
A. de VRIES J. C. BREARLEY & P. M. TAYLOR 《Journal of veterinary pharmacology and therapeutics》2009,32(4):353-358
Volatile agent-induced hypotension may contribute to anaesthetic-related morbidity and mortality in horses. Dobutamine is commonly used to support arterial blood pressure (ABP) but little is known about its cardiovascular effects under clinical conditions. The aim of this clinical study was to elucidate the relationship between cardiovascular function and dobutamine infusion in isoflurane-anaesthetized horses. Forty-four horses anaesthetized for a variety of surgical procedures were studied. Premedication with acepromazine, methadone and detomidine was followed by induction of anaesthesia with ketamine and midazolam. Anaesthesia was maintained with isoflurane vaporized in oxygen. Routine anaesthetic monitoring was applied and cardiac output was measured by lithium dilution. Dobutamine was infused to maintain mean ABP above 70 mmHg. The relationship between dobutamine infusion rate, heart rate (HR), ABP and cardiac index was investigated immediately prior to ( T 0 ) and 15 min ( T 1 ) after dobutamine infusion started, followed at 30 min intervals ( T 2 , etc.). Arterial blood pressure increased significantly after dobutamine infusion started, HR and cardiac index increased significantly only with dobutamine infusion in combination with surgical stimulus. Although isoflurane decreases blood pressure mainly by vasodilation, dobutamine is an effective treatment for hypotension under clinical conditions in isoflurane-anaesthetized horses. The effect of dobutamine is not directly proportional to dose and surgical stimulus probably contributes to the cardiovascular improvement. 相似文献
962.
K.A. Hart G.L. Heusner N.A. Norton M.H. Barton 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(2):344-351
Background: Hypothalamic-pituitary-adrenal (HPA) axis function is dynamic in the neonatal foal. The paired low dose/high dose cosyntropin (ACTH) stimulation test allows comprehensive HPA axis assessment, but has not been evaluated in neonatal foals.
Hypothesis: Foal age will significantly affect cortisol responses to a paired 10 and 100 μg dose cosyntropin stimulation test in healthy neonatal foals.
Animals: Twenty healthy neonatal foals.
Methods: HPA axis function was assessed in 12 foals at birth and at 12–24, 36–48 hours, and 5–7 days of age. At each age, basal cortisol and ACTH concentrations were measured and cortisol responses to 10 and 100 μg cosyntropin were assessed with a paired ACTH stimulation test protocol. Eight additional 36–48-hour-old foals received saline instead of 10 μg cosyntropin in the same-paired ACTH stimulation test design.
Results: At birth, foals had significantly higher basal cortisol and ACTH concentrations and higher basal ACTH : cortisol ratios compared with foals in all other age groups. A significant cortisol response to both the 10 and 100 μg doses of cosyntropin was observed in all foals. The magnitude of the cortisol response to both doses of cosyntropin was significantly different across age groups, with the most marked responses in younger foals. There was no effect of the paired ACTH stimulation test design itself on cortisol responses.
Conclusions and Clinical Importance: A paired 10 and 100 μg cosyntropin stimulation test can be used to evaluate HPA axis function in neonatal foals. Consideration of foal age is important in interpretation of HPA axis assessment. 相似文献
Hypothesis: Foal age will significantly affect cortisol responses to a paired 10 and 100 μg dose cosyntropin stimulation test in healthy neonatal foals.
Animals: Twenty healthy neonatal foals.
Methods: HPA axis function was assessed in 12 foals at birth and at 12–24, 36–48 hours, and 5–7 days of age. At each age, basal cortisol and ACTH concentrations were measured and cortisol responses to 10 and 100 μg cosyntropin were assessed with a paired ACTH stimulation test protocol. Eight additional 36–48-hour-old foals received saline instead of 10 μg cosyntropin in the same-paired ACTH stimulation test design.
Results: At birth, foals had significantly higher basal cortisol and ACTH concentrations and higher basal ACTH : cortisol ratios compared with foals in all other age groups. A significant cortisol response to both the 10 and 100 μg doses of cosyntropin was observed in all foals. The magnitude of the cortisol response to both doses of cosyntropin was significantly different across age groups, with the most marked responses in younger foals. There was no effect of the paired ACTH stimulation test design itself on cortisol responses.
Conclusions and Clinical Importance: A paired 10 and 100 μg cosyntropin stimulation test can be used to evaluate HPA axis function in neonatal foals. Consideration of foal age is important in interpretation of HPA axis assessment. 相似文献
963.
In the Upper Zambezi and Okavango ecoregions, Brycinus lateralis, Hepsetus cuvieri, Schilbe intermedius and Serranochromis macrocephalus are important in subsistence fisheries, while S. intermedius and S. macrocephalus are often caught in commercial catches. Despite their importance, there is little information on their age and growth and age validation. Growth zone deposition rate in otoliths of B. lateralis, H. cuvieri, S. intermedius and S. macrocephalus was validated as annual using edge analysis in this study. Annual deposition of growth zones was corroborated by a fluorochrome marking experiment. Both methods demonstrated that one growth zone was deposited annually. Edge analysis demonstrated that the highest proportion of opaque zones was detected between August and December, most likely as a result of slow growth during lower temperature water during the preceding winter. With growth zone deposition validated as annual, future research should focus on determining the growth, maturity and mortality rates of these species, parameters which are important for their management. 相似文献
964.
965.
966.
Pharmacokinetics of gamithromycin after intravenous and subcutaneous administration in pigs 总被引:1,自引:0,他引:1
H. Wyns E. MeyerE. Plessers A. WatteynS. De Baere P. De BackerS. Croubels 《Research in veterinary science》2014
The aim of this study was to investigate the pharmacokinetic properties of gamithromycin in pigs after an intravenous (i.v.) or subcutaneous (s.c.) bolus injection of 6 mg/kg body weight. The plasma concentrations of gamithromycin were determined using a validated high-performance liquid chromatography–tandem mass spectrometry method, and the pharmacokinetics were noncompartmentally analysed. 相似文献
967.
H. Petznek M. Kleiter A. Tichy A. Fuchs-Baumgartinger C. Hohenadl 《Research in veterinary science》2014
Therapy of cats suffering from feline injection site sarcomas (FISS) is still a challenging problem, as the recurrence rate after surgery is up to 70%. Four FISS-derived primary tumour cell lines and corresponding xenograft tumour mouse models were established to evaluate the efficacy of a concomitant chemo-/radiation therapy with doxorubicin. In vitro, strongly depending upon the timing of administration, pre-treatment with 0.25 µmol doxorubicin resulted in a significant enhancement of radiation-induced (3.5 Gy) tumour cell death. This result was confirmed in vivo, where only the combined chemo-/radiation therapy resulted in a significant reduction in tumour growth compared to the respective mono-therapies with either doxorubicin or radiation. These results support the use of the concomitant chemo-/radiation therapy for adjuvant treatment of FISS, particularly in advanced or recurrent disease where surgery alone is no longer feasible. 相似文献
968.
J.J. Fourie A. Joubert M. Labuschagné F. Beugnet 《Comparative immunology, microbiology and infectious diseases》2014
A 28-day study was conducted to assess the dynamic of blood feeding by Rhipicephalus sanguineus ticks on dogs treated or not with a novel topical combination of fipronil, amitraz and (S)-methoprene. Dogs were infested weekly through exposure to ticks in crates for 4 h. Ticks were then counted in the crates at 2 h and 4 h post dog exposure. Ticks were also counted and removed from the dogs at 2 h, 4 h, 6 h, 12 h and 24 h post tick exposure. The inhibition of blood feeding was assessed by both tick quantification and designing and performing a quantitative PCR (qPCR) to detect the canine hydroxymethylbilane synthase (HMBS) gene in ticks. The percentage of repellency sensu lato based on the ticks collected in crates at 2 h varied from 4.7% at day 28 to 48.3% at day 7. The immediate mortality rate of the ticks expelled at 2 h varied from 1.5% at day 21 to 31.7% at day 7. The efficacy calculation showed that the acaricidal combination started to kill ticks in as little as 2 h. The average efficacy reached 90.0% at 12 h post crate challenges and 100% at 24 h post exposure in crates. The inclusion of an internal amplification control was used to ensure that no significant template-derived PCR inhibition (≤6.2%) affected the overall results. The reduction of blood feeding was significant at 4 h (>80.0%) and >99.0% at 24 h post tick exposure in the crate. The high repellency rate and the lethal efficacy of CERTIFECT® resulted in significantly fewer live attached ticks, consequently reducing blood intake and fluid exchanges. 相似文献
969.
A.M.B. Veldhuis I.M.G.A. Santman-Berends J.M. Gethmann M.H. Mars L. van Wuyckhuise P. Vellema M. Holsteg D. Höreth-Böntgen F.J. Conraths G. van Schaik 《Preventive veterinary medicine》2014
Schmallenberg virus (SBV), a novel orthobunyavirus that rapidly spread throughout north-western Europe in 2011, caused congenital malformations in lambs and goat kids (Van den Brom et al., 2012) and newborn calves (Hoffmann et al., 2012). The impact of the SBV epidemic seemed limited however, in terms of the number of affected herds with malformed offspring (European Food Safety Authority, 2012b). Nevertheless, little is known with regard to the overall within-herd impact of SBV infection. The objective of the current study was to quantify the impact of the 2011 SBV epidemic on the productivity of dairy cattle in the Netherlands and the district of Kleve, Germany. 相似文献
970.